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배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성
홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.
Applying Artificial Neural Network to Fault Diagnosis of Analogue Circuit
Yang, ZhEng Rong,Musgrave, G. 대한전자공학회 1993 ICVC : International Conference on VLSI and CAD Vol.3 No.1
In this paper, we have applied artificial neural networks to fault diagnosis of analogue circuits. The experiments presented have proved that the artificial neural network can be used both for catastrophic faults and parametric faults. We have applied three artificial neural network, models to the fault diagnosis of DC, frequency, parametric, and steady state response. Two important techniques are introduced for the selection of artificial neural network models. These two techniques are: pattern distinction and quantity factor graph.
Zheng Liu,Jianjun Chen,Lei Cheng,Huabin Li,Shixi Liu,Hongfei Lou,Jianbo Shi,Ying Sun,Dehui Wang,Chengshuo Wang,Xiangdong Wang,Yongxiang Wei,Weiping Wen,Pingchang Yang,Qintai Yang,Gehua Zhang,Yuan Zhan 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.2
The current document is based on a consensus reached by a panel of experts from the Chinese Society of Allergy and the Chinese Society of Otorhinolaryngology-Head and Neck Surgery, Rhinology Group. Chronic rhinosinusitis (CRS) affects approximately 8% of Chinese adults. The inflammatory and remodeling mechanisms of CRS in the Chinese population differ from those observed in the populations of European descent. Recently, precision medicine has been used to treat inflammation by targeting key biomarkers that are involved in the process. However, there are no CRS guidelines or a consensus available from China that can be shared with the international academia. The guidelines presented in this paper cover the epidemiology, economic burden, genetics and epigenetics, mechanisms, phenotypes and endotypes, diagnosis and differential diagnosis, management, and the current status of CRS in China. These guidelines—with a focus on China—will improve the abilities of clinical and medical staff during the treatment of CRS. Additionally, they will help international agencies in improving the verification of CRS endotypes, mapping of eosinophilic shifts, the identification of suitable biomarkers for endotyping, and predicting responses to therapies. In conclusion, these guidelines will help select therapies, such as pharmacotherapy, surgical approaches and innovative biotherapeutics, which are tailored to each of the individual CRS endotypes.
Yang Zheng,Xiaotao Gao,Changdong Sheng 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.4
The present work aimed at combustion optimization of a 1,000MW tower-type ultra-supercritical boiler co-firing a lean coal with bituminous coals for reducing NOX emission particularly at low load operations. Historic operation data were systematically analyzed to investigate the characteristics of NOX emission. Through comparing between lean coal co-firing and sole bituminous coal firing, it was confirmed that, besides the big difference in quality and combustion characteristics of the lean coal from the bituminous coals, the excess air ratio in main combustion zone had a significant effect on NOX emission. Keeping the ratio at properly lower level achieved lower NOX emissions at low load operations. Based on the analyses, in-situ tests successfully brought NOX emissions of co-firing down close to 300mg/m3 at the load of 700MW, demonstrating the effectiveness of combustion optimization for controlling NOX emissions at partial load operation of co-firing.
Time-oriented Perception of Environmental design
Zheng Shu Yang,Li Yan 국민대학교 동양문화디자인연구소 2010 Journal of Oriental Culture&Design Vol.2 No.1
Time-oriented Perception of Environmental design is based on the foundation of time and space. Following then key perception of environmental esthetics, design puts emphasis on the time-oriented experience. Because of the unique understanding of time and space in Eastem culture, Chinese traditiona1 architecture is the manifestation of this perception.
The effect of SiO2 on the performance of inorganic sludge-based structural concretes
Zheng Xun Yang,Na Ra Ha,이종국,Min Sik Jang,황규홍 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.3
As a relatively new material, geopolymer concrete offers benefits as a construction material for sustainable development. It utilizes waste materials such as recycled concrete sludge, fly ash, etc. It has a very low rate of green house gas emission when compared to ordinary Portland cement. In this study, the components of geopolymer are concrete sludge, metakaolin and water glass. NaOH was used as an alkaline activator. To improve the mechanical properties, silica fume was added as a bonding matrix from 0%-10% to replace part of the concrete sludge, and the specimens were cured in air, then their mechanical properties such as compressive strength and bending strength were measured and their microstructures were investigated.
Friendship Influence on Mobile Behavior of Location Based Social Network Users
Yang Song,Zheng Hu,Xiaoming Leng,Hui Tian,Kun Yang,Xin Ke 한국통신학회 2015 Journal of communications and networks Vol.17 No.2
In mobile computing research area, it is highly desirable to understand the characteristics of user movement so that the user friendly location aware services could be rendered effectively. Location based social networks (LBSNs) have flourished recently and are of great potential for movement behavior exploration and datadriven application design. While there have been some efforts on user check-in movement behavior in LBSNs, they lack comprehensive analysis of social influence on them. To this end, the socialspatial influence and social-temporal influence are analyzed synthetically in this paper based on the related information exposed in LBSNs. The check-in movement behaviors of users are found to be affected by their social friendships both from spatial and temporal dimensions. Furthermore, a probabilistic model of user mobile behavior is proposed, incorporating the comprehensive social influence model with extent personal preference model. The experimental results validate that our proposed model can improve prediction accuracy compared to the state-of-the-art social historical model considering temporal information (SHM+T), which mainly studies the temporal cyclic patterns and uses them to model user mobility, while being with affordable complexity.
Zheng Chen,Gama Yang,Tingzhen Mu,Maohua Yang,Nadia A. Samak,Sumit Peh,Yunpu Jia,Xuemi Hao,Xuhao Zhao,Jianmin Xing 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-
The highly efficient performance of H2S absorption is the crucial indicator for haloalkaliphilic biologicaldesulfurization (HBDS) because it immediately concerns the H2S removal efficiency and pH change ofalkaline solutions. Therefore, we investigated the effect of operating parameters on the H2S absorption’sperformance under haloalkaline conditions. The gas–liquid ratio and packing height significantly improveH2S removal efficiency, from 80% to 90% and 66% to 99%, respectively. The absorption temperature had atrivial impact on the H2S removal efficiency, and the maximum value appeared at 45 C. Additionally, alloperating parameters caused pH changes that varied in the acceptable range (0.1 to 0.5) during theabsorption process. A rate-based model was successfully developed to predict the haloalkaliphilic H2Sabsorption process accurately. Moreover, this model could be implemented to effectively evaluate theHBDS system’s stability and provide reliable theoretical guidance for the industrial HBDS process toensure good process stability.
Zheng, Tao,Zhang, Tian-biao,Wang, Chao-liang,Zhang, Wei-xing,Jia, Dong-hui,Yang, Fan,Sun, Yang-yang,Ding, Xiao-ju,Wang, Rui Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6
Icariside II (ICA II) is used in erectile dysfunction treatment. Adipose tissue-derived stem cells (ADSCs) are efficient at improving erectile function. This study aimed to explore the action mechanism of ADSCs in improving erectile function. ADSCs were isolated from the adipose tissues of rats. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay. The expressions of mRNA and protein were determined separately through qRT-PCR and western blot. The endogenous expressions of related genes were regulated using recombinant plasmids and cell transfection. A Dual-Luciferase Reporter Assay was performed to determine the interaction between miR-34a and STAT3. Rat models with bilateral cavernous nerve injuries (BCNIs) were used to assess erectile function through the detection of mean arterial pressure (MAP) and intracavernosal pressure (ICP). ICA II promoted ADSCs' proliferation and differentiation to Schwann cells (SCs) through the inhibition of miR-34a. Suppressed miR-34a promoted the differentiation of ADSCs to SCs by upregulating STAT3. ICA II promoted the differentiation of ADSCs to SCs through the miR-34a/STAT3 pathway. The combination of ICA II and ADSCs preserved the erectile function of the BCNI model rats. ADSCs treated with ICA II markedly preserved the erectile function of the BCNI model rats, which was reversed through miR-34a overexpression. ICA II promotes the differentiation of ADSCs to SCs through the miR34a/STAT3 pathway, contributing to erectile function preservation after the occurrence of a cavernous nerve injury.