Self-Assembling Peptides and Their Application in the Treatment of Diseases

Lee,,Trinh,,Yoo,,Shin,,Lee,,Kim,,Hwang,,Lim,,Ryou, MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.23

<P>Self-assembling peptides are biomedical materials with unique structures that are formed in response to various environmental conditions. Governed by their physicochemical characteristics, the peptides can form a variety of structures with greater reactivity than conventional non-biological materials. The structural divergence of self-assembling peptides allows for various functional possibilities; when assembled, they can be used as scaffolds for cell and tissue regeneration, and vehicles for drug delivery, conferring controlled release, stability, and targeting, and avoiding side effects of drugs. These peptides can also be used as drugs themselves. In this review, we describe the basic structure and characteristics of self-assembling peptides and the various factors that affect the formation of peptide-based structures. We also summarize the applications of self-assembling peptides in the treatment of various diseases, including cancer. Furthermore, the in-cell self-assembly of peptides, termed reverse self-assembly, is discussed as a novel paradigm for self-assembling peptide-based nanovehicles and nanomedicines.</P>

조혈모세포이식 환자에서 발생한 Cytomegalovirus 질환의 특징 : 일개 대학변원에서 최근 10년간의 경험

최수미,이동건,박선희,김시현,김유진,민창기,김희제,이석,최정현,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2009 감염과 화학요법 Vol.41 No.1

Background : Studies on cytomegalovirus (CMV) diseases in Korean hematopoietic stem cell transplant (HSCT) recipients are lacking and do not reflect the recent trends of advances and changes. Therefore, we tried to analyze the clinical features of CMV diseases in HSCT recipients over the past 10 years at a tertiary university hospital in Korea. Methods : Retrospective review of medical records was done for all adult HSCT patients who received transplant at the Catholic HSCT Center from January 1998 to January 2008. Results : Forty-four cases (2.2%) of CMV diseases were identified. CMV pneumonia was diagnosed in 17 patients, retinitis in 16 patients, enterocolitis in 7 patients, esophagitis 1 patient, gastritis in 1 patient, duodenitis in 1 patient, and hepatitis in 1 patient. The median onset of symptom was 90 days after transplantation. Late CMV diseases accounted for 47.7%. CMV related death varied from 0 to 58.8% according to the involved organ. CMV retinitis was diagnosed relatively later in the course of transplantation mostly in patients who had chronic graft versus host disease (GVHD). On the contrary, CMV enterocolitis mainly occurred in patients who suffered from acute GVHD. The overall concurrent CMV reactivation was documented to be 63.6%: the concurrent CMV reactivation was observed only in 37.5% of patients with retinitis. Conclusions : We observed some differences in the pattern of CMV disease manifestation according to the involved organ and reconfirmed the fact that CMV pneumonia is the most common and fatal disease in HSCT recipients. Additionally, CMV retinitis was not uncommon in HSCT recipients. Since specific marker does not exist in predicting retinitis, regular ocular examination should be done thoroughly, especially in patients with chronic GVHD.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

악성 림프종 환자에서 발생한 다형홍반

여운철,조광현,이유신,허대석,이승철 ( Un Cheol Yeo,Kwang Hyun Cho,Yoo Shin Lee,Dae Seog Heo,Seung Chul Lee ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Erythema Multiforme A ssociated with Malignant Lymphoma Un Cheol Yeo, M.D., Kwang Hyun Cho, M.D., Yoo Shin Lee, M.D., Dae Seog Heo*, M.D., Seoung Chul Lee**, M.D Department of Dermatology and Internal Medicine*, College of Medicine, Seoul National University, Seoul, Korea, Department of Dermatology**, College of Medicine, Inha University, Kyung Gi Do, Korea We report a case of erythema multiforme associated with malignant lymphoma. Fifty-six-year-old male patient visited our clinic complaining generalized skin lesions which were compatible with erythema multiforme clinically and histopathologically. The skin lesions were treatment-resistant, recurrent and persistent for 3 years. In consideration of old age, atypical features of erythema multiforme and no other demonstrable etiology of erythema multiforme, we studied for internal malignancy. Malignant lymphoma at the duodenum was revealed by UGI series, abdominal CT, and endoscopic biopsy. After chemotherapy, the tumor mass and active skin lesions were cleared simultaneously. Erythema multiforme may be regarded as one of the paraneoplastic syndromes.

효율적인 유비쿼터스 정보 공동활용을 위한 국가적 USN 정보자원 관리체계 구축 정책에 관한 연구

이재호,안철현,김유정,신상철,이재용 한국정보사회진흥원 2006 정보화정책 Vol.13 No.2

정보화 사회를 지나 유비쿼터스 사회로 발전하기 위한 핵심 기술 중 하나인 USN은 최근 기상, 군사 분야 등을 중심으로 현장∙실증 시험이 활발히 추진되고 있으며, u-City 같은 실제 사업에 적용하는 방안도 적극 검토되고 있다. 하지만, 현재까지 실증시험 결과 USN간 연계를 위한 식별체계, 프로파일, 통신프로토콜, 데이터 형식 등이 상이하여 향후 상호 연동을 위한 추가 비용이 크게 증가할 것으로 예상되고, 이는 기존 정보화 사회에서 행정 정보 공동활용과 유사한‘선구축-후통합’에 따른 문제점을 답습할 가능성이 높아 보인다. 따라서 본 논문에서는 USN 정보공동활용을 위한 USN 기술표준 및 정보 관리 체계를 수립하여 전국적 USN 정보자원을 효율적으로 관리하기 위한 정책 방안과 이를 실현하기 위한 USIS(Ubiquitous Sensor Information System)1)를 함께 제안하였다.

韓國産 나비의 吸蜜植物에 關한 硏究

申裕恒,李光源 慶熙大學校 1988 論文集 Vol.17 No.-

This research covered the survey on the nectar plants attracted by a total of 79 species of butterflies inhabited in South Korea including Mt. Soˇlak, Mt. Myoˇngii, Mt. Choˇnma, Kangchon, Kwangnuˇng, Namhansansoˇng, Is. Hong, Is. Ulluˇng, etc. for 6 years from 1983 through 1988. The results obtained were as follows: 1. A total of 48 classes of 105 species of nectar plants on which 79 species(41%) of butterflies fed among 193 species inhabited in South Korea were observed. 2. Of the nectar plants in which butterflies fed, the plants of Compositae were rated the highest at 14% of the nectar plants. The second were the plants of Rosaceae(13%), the third were the plants of Cruciferae(11%). 3. In examining the preferences of the butterflies to the colors of nectar plants, it was found that white color was preferred by 43% of the butterflies in the highest rate, followed by blue color os 20% of the butterflies, yellow color by 10.5% of the butterflies and the red color by 8% of the butterflies at the lowest rate. 4. Staphylea bumalda and Rubus idaeus var. microphyllus were the shrubs on which the butterflies most frequently coming to feed. And Erigeron annuus and Cirsium japonicum var. ussuriense were the weeds on which the butterflies were most frequently coming to feed. 5. Pieris rapae showed the widest range of diversity to nectar plants by feeding on 18 species nectar plants. The second was Mellicta athalia which fed on 17 species of nectar plants. The the third was Artephaneura alcinous which fed on 16 species of nectar plants.

Poly(ρ-phenylenesulfide)와 Poly(ρ-phenylene)으로부터 제조된 탄소의 리튬 이온 2차전지 anode 재료로서의 전기화학적 특성

유덕영,이주성,박수길,변지형,류신환,정윤이 한양대학교 에너지·환경기술연구소 1998 에너지·環境技術論文集 Vol.4 No.-

Poly(p-phenylenesulfide)와 poly(p-phenylene)으로부터 제조된 탄소를 리튬이온 2차전지용 음극재료로 사용하여 전기화학적특성을 연구하였다. 이들 고분자들을 질소 분위기하에서 승온속도 2℃/min로 1000℃까지 상승시킨 후, 1시간 동안 탄화시켜 탄소를 제조하였다. Poly(p-phenylene)으로부터 제조된 탄소가 보다 더 규칙적인 구조를 가지기 때문에 Poly(p-phenylenesulfide)으로부터 제조된 탄소보다 더 많은 용량과 충방전 효율을 나타내었다. 수산화리튬과 염화주석(Ⅱ)을 첨가하여 용량의 증가와 충방전 효율의 증가를 가져올 수 있었다. 이중에서 poly(p-phenylene)에 염화주석(Ⅱ)을 첨가시켜 제조된 탄소가 가장 큰 충방전 용량과 충방전 효율의 향상을 가져왔다. Carbon inaterials manufactured from poly(p-phenylene sulfide) and poly(p-phenylene), were studied on electrochemical characteristics as anode materials for lithium ion secondary battery. These polymer precursors were heat treated for 1hr at 1000℃ with the rate 2℃/min under nitrogen atmosphere. Carbon manufactured from poly(p-phenylene) showed higher capacity and coulomb efficiency of charge/discharge than carbon from poly(p-phenylene sulfide) because the former has better ordered structure. Carbon manufactured from polymer precursors adding stannous chloride or lithium hydroxide showed higher capacity and better efficiency of charge/discharge. Also, carbon manufactured by adding stannous chloride to poly(p-phenylene) showed the highest capacity and efficiency of charge/discharge.

Cis-Dichlorodiammineplatinum이 Mouse 간장의 인산염 분해효소활성에 미치는 영향

유신철,이동준,정호삼,이규식 漢陽大學校環境科學硏究所 1987 環境科學論文集 Vol.8 No.-

cis-Dichlorodiammineplatium(Ⅱ)(cis-DDP)은 최근에 개발된 효과적인 종양화합 요법제인 금속화합물로서 그 작용기작은 아직 불명하나 bifunctional alkylating agent와 유사한 작용 기전을 갖는 것으로 알려져 있다. cis-DDP는 체내에서 가수분해되어 DNA와 반응함을 핵산의 합성을 억제하여 암세포 뿐만 아니라 정상세포에도 영향을 미쳐 출혈성장염, 임파조직과 골수의 저세포증, 신손상, 단백뇨 및 혈뇨등의 부작용을 유발한다. 이에 저자는 cis-DDP가 mouse 간장에서 세포독성으로 작용하여 간실질세포의 변성을 일으키리라 사료되어 간실질세포내의 acid phosphatase, alkaline phosphstase 및 aden-osine triphosphatase의 변동을 조직화학적으로 관찰하였다. 실험동물은 DDY계의 웅성(체중 20g)mouse를 사용하였으며 체중 kg당 6mg의 cis-DDP를 투여한 후, 6시간, 12시간, 24시간 및 48시간 경과후에 희생하여 간조직을 적출하고 10% formalin용액에 고정하고 cryostat로 16㎛두께로 동결절편을 만들었다. 조직화학적인 연구를 위하여 acid phosphatase와 alkaline phosphatase의 활성은 Gomori 방법으로 그리고 adenosine triphosphatase의 활성은 Wachstein-Meisel 방법으로 이들 효소활성을 관찰하여 다음과 같은 결과를 얻었다. 1. Acid phosphatase 활성은 간소엽주변대에서 cis-DDP투여 6시간 경과군의 mouse에서는 약한 양성반응을 12시간 경과군의 mouse에서는 중등도의 양성반응을 48시간 경과군의 mouse에서는 약한 양성반응을 나타내었다. 2. Alkaline phosphatase 활성은 간소엽 주변대에서 cis-DDP투여 12시간, 24시간, 및 48시간 경과군의 mouse에서 중등도의 양성반응을 나타내었다. 3. Adenosine erihosphatase 활성은 간소엽 중심대에서 cis-DDP투여 6시간 경과군의 mouse에서 미약한 양성반응을 나타내었고, 12시간 경과군의 mouse에서는 중등도의 양성반응을 나타내었고, 24시간 및 48시간 경과군의 mouse에서는 각각 중등도 및 강한 양성반응을 나타내었다. 이상의 소견을 종합한바 cis-DDP의 투여로 mouse의 간장 손상을 일으켜 acid phosphstase, alkaline phospatase 및 adenosine triphosphatase 활성은 일시적으로 저하되었다가 회복되는 것으로 사료된다. cis-Dichlorodiammineplatinnm(Ⅱ) (cis-DDP), known as cisplatin, is a metallic compound that has recently been developed as an effective cancer chemotherapeutic agent. The precise mechanism of action of cis-DDP is still unknown. Similarities between the mechanism of action of this drug and bifunctional alkylating agents have been suggested. Upon entering the cell, the platinum compounds will become hydrolyzed, and then they can react with DNA. They act on tumor cells as well as normal cells. Side effects included severe hemorrhagic enterocolitis, severe hypocellularity of the bone marrow and lymphoid tissues, renal lesions, proteinuria and (microscopic henaturia). In this experiment, the author studied the effect of cis-DDP on the mouse liver, histochemically observing the changes in the activities of acid phosphatase, alkaline phosphatase, and adenosine triphosphatase. Male mice (the weight 20g), DDY strain, were used as experimental animals. The experimental animals were sacrificed at 6,12,24 and 48 hours after administration of 6mg of cis-DDP per kilogram of body weight. The specimens obtained from the liver were fixed in 10% formalin solution and sliced at 16㎛ thickness in a frozen cryostat. The activities of acid and alkaline phosphatases were observed by the Gomori's method and the activity of adenosine triphosphatase was observed by the Wachstein Meisel's method for histochemical study. The results are as follows. 1. The acid phosphatase activity was weakly positive at 6 hours and moderately positive at 12 hours and weakly positive at 48 hours after administration in the peripheral zone of the hepatic lobule. 2. The alkaline phosphatase activity was decreased to moderately positive at 12,24 and 48 hours after administration in the peripheral zone of the hepatic lobule. 3. The adenosine triphosphatase activity was trace positive at 6 hours after administration in the central zone of the hepatic lobule and moderately positive at 12 hours after admnistration in the central and peripheral zone of the hepatic lobule. It is suggested that cis-DDP causes changes in activities of acid phosphatase, alkaline phosphatase and adenosine triphophsatase in the liver, due probably to the cytotoxic effect in the parenchymal cells of the liver in mice.

주정중독 환자와 정상음주자에 대한 감정상태와 자기 지각, 가정환경, 사회적 지지에 관한 비교 연구

이정식,한광수,이유상,한일우,신용구 용인정신의학연구소 1997 용인정신의학보 Vol.4 No.1