장내의 탄수화물과 지방 흡수 억제를 통한 체지방 및 비만 개선 효과에 관한 연구

정은희,윤승원,이홍석,윤유식,유경미,황인경 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.1

In a previous study, a dietary supplement was developed in our lab using natural herbal extracts against digest enzyme activity in GI tract for weight control. This natural herbal extracts could regulate absorption of glucose and lipid by the inhibition of digest enzyme activity. In this study, we screened the natural herbs that inhibit glucoamylase activity and developed an water extract of cinnamon. The cinnamon extract delayed and decreased the increment of carbohydrate degradation through the inhibition of glucoamylase activity in vitro. Fifty volunteers were subjected to the intake of the herbal extracts by taking twice a day for 60 days. As a result, the treated subjects lost 3kg of body weight and 3.5kg of body fat mass after the treatment. Furthermore, the body mass index and waist size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of cinnamon and natural herbal extract improves the obesity by the decrement of body weight and body fat mass.

노인 낮병원의 심리사회적 치료 프로그램이 노인의 신경인지기능과 우울 증상에 미치는 효과

유계준,송정은,오병훈,안석균,이홍식,오희철,구은형,황혜숙,이은철 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

노인에게 있어서 신경인지기능의 저하와 우울 증상은 가장 흔한 장애이며 신체적, 정신적인 면에 많은 영향을 미치고 있다. 또한 이러한 장애중 비임상군에서 심리사회적 치료개입의 효과가 보고되고 있다. 이러한 측면에서 지역사회에서 노인에게 제공되는 심리사회적 치료개입의 중요성이 높아지고 있다. 이전의 연구에서는 대상이 특정 집단이거나, 비교군이 없거나, 치료효과에 대한 추적 관찰이 이루어지지 않는 등의 제한점이 있었으며, 현재 국내의 노인 낮병원은 소수에 불과하고 이러한 낮병원의 치료효과에 대한 연구는 거의 없는 실정이다. 따라서 본 연구는 지역사회 정신보건센터에서 운영하는 노인 낮병원에 참가하는 노인들을 대상으로 낮병원의 심리사회적 프로그램이 노인들의 신경인지기능과 우울 증상에 미치는 효과와 이에 대한 유지 효과의 유무를 알아보고자 하였다. 경기도 광주군 정신보건센터의 노인 낮병원에 참여한 노인을 치료군으로, 치료를 거부하거나 치료 중 탈락한 노인을 비교군으로 하여 10주간의 낮병원 치료전후와 치료종결 후 10주에 신경인지기능은 한국형 간이 정신상태검사(이하 MMSE-K)로, 우울 증상은 단축형노인우울척도(이하 SGDS)로 평가하여 두 군을 비교하였고 치료군의 치료 직후와 치료종결 후 10주를 비교하였다. 연구결과 노인 낮병원의 심리사회적 치료가 시간에 따른 SGDS점수 변화에 통계학적으로 유의한 영향을 미쳤으며, MMSE-K점수 변화에는 통계학적으로 유의한 영향을 미치지 않았다. 또한 10주 추시가 가능했던 치료군 중 약 43%가 10주 후 SGDS가 증가하여 호전되었던 우울증상의 악화를 보였다. 본 연구 결과 노인 낮병원의 심리사회적 치료 프로그램 개입은 노인에게 있어서 우울 증상을 호전시키며 신경인지 기능에는 직접적인 영향을 미치지 않는 것을 알 수 있었다. 또한 상당수에서 심리사회적 치료 개입으로 인한 우울 증상의 호전이 유지되지 않음을 알 수 있었다. 이는 노인우울증상의 치료에 낮병원의 심리사회적 치료 프로그램이 효과적일 수 있음과 치료 후 이를 유지하기 위한 부가적인 치료개입의 필요성을 시사한다. Cognitive impairment and depression are the most common symptoms affecting the elderly on physical and mental states. Studies have shown that these symptoms in mild cases are improved by psychosocial intervention, emphasizing the importance of therapeutic intervention provided in the community. The purpose of this study was to investigate whether the psychosocial program at a geriatric day care service has any therapeutic effect on the cognitive function and depressive symptoms in the elderly and whether such effect can be maintained. The experimental group was composed of 37 elderly patients attending the day care service at the mental health center located in Kwangju county, Kyonggi province. The control group was composed of 22 elderly patients who either refused intervention or who dropped out of the program. Cognitive function was assessed with the Korean version of the Mini-Mental State Exam(MMSE-K), and depressive symptoms were assessed with the Short-form Geriatric Depression Scale(SGDS). The experimental group and the control group were compared on the score of on each test, which was administered before and after the program and 10 weeks after discontinuation of program. In the experimental group, the scores acquired immediately after the program and at 10 weeks of follow-up were also compared. The results showed that psychosocial intervention at a geriatric day care service was significantly associated with the change in SGDS scores dependent on time but not significantly associated with the change in MMSE-K scores. In the experimental group whose follow-up assessment was possible, it was further shown that 43% of patients had increased SGDS scores 10 weeks after the program ended, indicating that depressive symptoms had worsened. This study suggests that psychosocial therapeutic intervention in the geriatric day care service improve depressive symptoms but not cognitive functions in the elderly. In addition, for a considerable percentage of subjects in the experimental group, the improvements in depressive symptoms were not sustained after the intervention was withdrawn. These findings proposes a need of strengthening therapeutic intervention to maintain such effect.

水稻의 適正 用水量 算定에 關한 硏究 : 葉水面 蒸發量을 中心으로 For Amount of Evapotranspiration

黃龍鎭,宋錫銀,鄭鳳守,朴魯奭,李裕根,李永日,黃光性 진주산업대학교 1976 論文集 Vol.14 No.-

1. 試驗기간의 氣象現況은 Table 1과 같다. 2. 벼의 엽면증발량은 活着後 점차 增加하여 第8기에 Peak로 되며 이때의 1日 엽면 증발량은 5.16mm/day로 되었다가 점차 減少現象을 보인다. 3. 엽면증발량은 第8期에 全 葉面증발량의 17.5%에 達한다. 4 벼의 증산비는 282이였다. 5. 株間수면증발량은 Table 3과 같으며 葉面증발량과는 負(一)의 相關關係가 있음을 보였다. 6. 증발계증발량과 엽수면증발량과의 比는 1.43이였다. 7. 증발계증발량과 葉面증발량과의 비는 0.89이였다. 8. Blaney- Criddle method에 依한 k値는 Table 5와 같다. 9. 증산强度는 총계 1021로, 증발산强度는 총계 1141.8로 나타났다. The resuts of the study on consumption use of irrigated water in pabdy field during the growing stage of gaddy rice are summarized as follow. 1. Amount of transpiration of paddy rice increases gradually after trasplantation and reaches peak on 8th growing period and amount of traspiration reached on 5.16mm/day, then it decreases gradual1y after that. 2. Trnspirstion reached l7. 5% of amount of transpiration at 8th growing period. 3. Transdiration ratio of paddy rice approximate1y 262. 4. As we know from Fig. 2 correlation between the amouut of everporation from water surface in paddy field and amount of trans piration shows high negative. 5. Ratio of between evaportion and evapotranspiration is 1.43. 6. Ratio of between evaportion and transpiration is 0.89. 7. The k value of Blaney-Cliddle methed shows Tab1e 6. 8. Transpion and evaoptranspiration intennsities is each other 1021 and 1141.8.本 試驗은 엽수면증발량 株間수면증발량을 實測하였으며 이 試驗에 여러 가지 未備한 点이 있는 것으로 思料되나 以上의 試驗結果를 要約하면 다음과 같다.

Intestinal lymphangiectasia in a Yorkshire terrier dog: clinical outcome, clinicopathological and histopathological findings

Duna Hwang,Chul Park,Byeong-Teck Kang,Jong-Hyun Yoo3,Soon-Wuk Jeong,Jung-Hyang Sur,박희명 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.3

A 7-year-old, 3.16 kg intact male Yorkshire terrier had the history of abdominal distension, diarrhea, andweight loss. On the basis of history takings, physical examination, laboratory tests, radiography, ultrasonography,exploratory laparotomy, and histopathological examination, the dog was diagnosed definitely as intestinallymphangiectasia. In this case, signs and results are consistent with those of other reports, but some clinicopathologicalfindings such as hypocalcemia and hypocholesterolemia are not. This means that the findings were not completelyin accord with the typical ones of intestinal lymphangiectasia in this dog. Prednisolone was prescribed to treat. However,it was not effective sufficiently. Thus, azathioprine was added to the regimen used in the first trial, and it was quiteefficient in inducing remission in intestinal lymphangiectasia. The clinical signs were improved to the combined therapy. This case report demonstrates that the combined therapy for intestinal lymphangiectasia can be used as an alternativeto only glucocorticoid therapy.

대형경유차 저공해기술 적용에 따른 나노입자 배출특성

임철수,류정호,엄명도,황진우,김예은 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.2

Diesel engines which emit a lot of PM and NOx have been known as a main air polluter. Especially, diesel particulate matters (DPM) including black smoke are hazardous air pollutants to human health and environment. The nations retaining advanced engine technologies have reinforced emission regulations. To meet these regulations diesel engine manufacturers have developed low-emission diesel engines, aftertreatment equipments, alternative fuel technologies and so on. In this study, particle number concentrations characteristics according to particle size and engine driving conditions were analyzed when these low-emission technologies were applied. There was a tendency of increasing particle number concentrations from heavy-duty diesel engines with increasing engine rpm and load rate. In the cases of CDPF (Catalytic Diesel Particulate Filter), CNG (Compressed Natural Gas) engine and ULSD (Ultra Low Sulfur Diesel) more than 99% of particle number concentration were removed.

Effect of Chlorella Diet Supplementation on Blood and Urine Cadmium Levels in Cadmium Poisoned Rats

김용호,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.3

This study was conducted to investigate the influence of chlorella diet supplementation. Blood accumulation and urine excretion levels were measured after the Sprague-Dawley (SD) rats were fed on a chlorella diet supplementation mixed with 40 ppm of CdCl₂. Four groups tested for blood accumulation and urine excretion levels. All four groups fed on a basic diet with a cadmium mixture. The diet for the first group contained only basic diet and the cadmium added to the drinking water. The diet for the three other groups contained cadmium to the drinking water, and 1%, 5% and 10% of chlorella added to the basic diet. A concentration of cadmium for the first group showed a 3.2±0.4 ㎍/l blood accumulatior. level and 41.5±32.9 ㎍/l urinary excretion level, and the second group, which was fed on the basic diet with 1% of chlorella added and cadmium to the drinking water showed a 1.5±0.6 ㎍/l blood level and only 14.1±1.6 ㎍/l urinary excretion level. The other two groups, which were fed on 5% and 10% of chlorella concentration and cadmium to the drinking water did not exhibit any notable effects greater than the group fed on 1% concentration of chlorella. The results suggest that the blood accumulation and urinary excretion of Cadmium are influenced by the chlorella diet supplementation from the concentration of 1% of the basic diet.

PDMS와 유리를 이용한 제한효 반응용 마이크로바이오칩 개발

허영선,염혜정,조철호,안유민,황승용 한양대학교 이학기술연구소 2005 이학기술연구지 Vol.8 No.-

최근 생화학 심험 분야에서 초미세 구조를 이용한 소형화 분야가 두드러지게 나타나고 있다. 소형화의 가증 큰 이점은 적은 양의 시료가 사용되기 때문에 실험 비용이 줄어들 뿐 아니라 실험 시간이 단축 된다는 것이다. 게다가 하나의 칩으로 바이오 실험의 모든 단계가 수행되는 랩 온어 칩의 통합을 위한 가능성을 나타내어 준다, 우리는 제한 효소 반응 실험에 응용하기 위해 새로운 마이크로바이오칩을 개발하였다, 이 마이크로 바이오칩은 PDMS와 유리를 사용해 제작하였고 총 실험 반응 양의 10㎕이다, 마이크로바이오칩을 이용한 제한효소 실험은 일반적으로 행해지는 실험 방법에 비해 반응 시간과 시료 양을 단축하는데 성공적이었다. In recent years, significant advances emerged in the area of miniaturzation of biochemical experiment using micro fabricated structures The key sdvantages of the miniaturization would be reduces assay costs due to the low consumpation of expensive reagent and increased experiment speed The additional benefits can be intergrated into Lab-on -a chip in which all the steps necessary in bio assays is performed in a single chip We have developed a novel Microbiochip for th application of Restriction Enzyme cut reaction The Microbiochip with reaction volum of 10 ㎕was fabricated using PDMS/glass The Restriction Enzyme cut reaciton using the microbiochip was successfully done with less time sample compared to the conventioal way

부산지역 미세먼지에 대한 건강 취약성 평가

이원정,황미경,김유근,Lee, Won-Jung,Hwang, Mi-Kyoung,Kim, Yoo-Keun 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.5

Objectives: This study seeks to evaluate the vulnerability assessment of the human health sector for $PM_{10}$, which is reflected in the regional characteristics and related disease mortality rates for $PM_{10}$ in Busan over the period of 2006-2010. Methods: According to the vulnerability concept suggested by the Intergovernmental Panel on Climate Change (IPCC), vulnerability to $PM_{10}$ is comprised of the categories of exposure, sensitivity, and adaptive capacity. The indexes of the exposure and sensitivity categories indicate positive effects, while the adaptive capacity index indicates a negative effect on vulnerability to $PM_{10}$. Variables of each category were standardized by the rescaling method, and each regional relative vulnerability was computed through the vulnerability index calculation formula. Results: The regions with a high exposure index are Jung-Gu (transportation region) and Saha-Gu (industrial region). Major factors determining the exposure index are the $PM_{10}$ concentration, days of $PM_{10}{\geq}50$, ${\mu}g/m^3$, and $PM_{10}$ emissions. The regions that show a high sensitivity index are urban and rural regions; these commonly have a high mortality rate for related disease and vulnerable populations. The regions that have a high adaptive capacity index are Jung-Gu, Gangseo-Gu, and Busanjin-Gu, all of which have a high level of economic/welfare/health care factors. The high-vulnerability synthesis of the exposure, sensitivity, and adaptive capacity indexes show that Dong-Gu and Seo-Gu have a risk for $PM_{10}$ potential effects and a low adaptive capacity. Conclusions: This study presents the vulnerability index to $PM_{10}$ through a relative comparison using quantitative evaluation to draw regional priorities. Therefore, it provides basic data to reflect environmental health influences in favor of an adaptive policy limiting damage to human health caused by vulnerability to $PM_{10}$.

주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.

국내 조혈모세포이식 환자에서 Human Cytomegalovirus gB 유전형의 분포와 질환과의 연관성 : 예비 보고

최수미,김진희,이동건,박선희,최정현,유진홍,박철민,이종욱,민우성,황응수,신완식,김춘추 대한감염학회 2007 감염과 화학요법 Vol.39 No.2

목적 : 사람 거대세포바이러스(Human cytomegalovirus, 이하 HCMV) glycoprotein B (gB)는 UL55 유전자에 의해 부호화되는 당단백으로, UL55 유전자의 염기서열변화에 따라 4가지 유전형으로 나뉜다. 본 연구에서는 국내 조혈모세포이식 환자들에서 HCMV gB 유전형의 분포와 그 특성을 알아보고, gB 유전형에 따라 특정 HCMV 질환 발생과 연관이 있는지 그 임상적 의미를 분석해 보고자 하였다. 재료 및 방법 : 동종 조혈모세포이식 환자 52명의 혈액검체 94개에 대해, Chou 등이 제시한 방법에 따라, 먼저 UL55 유전자 부위를 nested PCR로 증폭한 후, RsaI과HinfI으로 Restriction fragment length polymorphism(RFLP) 분석을 시행하였다. 결과 : gB type 1은 73.1% (38/52), gB type 2는 13.5%(7/52), gB type 3는 1.9% (1/52), gB type 1과 type 2에 의한 혼합감염은 9.6% (5/52)로 나타났다. gB type 4는 관찰되지 않았다. 1명(1.9%)에서 gB 유전형을 결정할 수 없었는데, RFLP 패턴으로 보아 Trincado 등이 제시한 gB type 7에 해당하는 것으로 생각되었고, 이 새로운 아형에 대해서는 현재 염기서열 분석 중이다. 52명 중 5명(9.6%)에서 HCMV 질환이 발생하였고, 3명에서 HCMV 폐렴, 1명에서 망막염과 위장관염, 나머지 1명에서 망막염이 발생하였다. 5명 중 HCMV 질환과 관련하여 사망한 예는 없었고, 감염된 HCMV는 모두 gB type 1이었다. HCMV gB유전형과 HCMV 질환 발생 사이에 유의한 연관성은 없었고, 단일 주에 의한 감염과 혼합감염에 따른 HCMV 질환발생 사이에도 유의한 연관성은 관찰되지 않았다. 자료 분석 중 gB type 2에 감염되어 있던 환자에서 gB type 1에 재감염 되면서 발열, 간효소 수치 상승 및 pp65 HCMV 항원혈증이 나타난 예가 있었다. 결론 : 연구결과 gB type 1이 아주 우세하고, gB type 4는 검출되지 않았으며, 혼합감염의 빈도가 비교적 낮은 분포를 보였다. 이는 외국의 보고와는 다른 국내 조혈모세포이식 환자에서의 독톡한 감염 양상으로 추정된다. 본 연구에서 HCMV gB 유전형과 질환 발생과의 연관성을 밝힐 수는 없었으나, HCMV 유전형에 대한 연구는 바이러스 감염의 발병기전이나 전파 경로 및 양식과 같은 역학적 연구에 중요한 자료가 될 것이다. 현재 더 많은 수의 조혈모세포이식 환자를 대상으로 연구가 진행 중에 있으며, 앞으로 다른 질환군의 환자나 건강한 잠복 감염자에서의 추가 연구가 필요할 것으로 생각된다. Background : Human cytomegalovirus (HCMV) glycoprotein B (gB) is the major envelope glycoprotein, encoded by the UL55 gene. Based on sequence variation in the UL55 gene, HCMV can be classified into four gB genotypes. Previous studies have suggested an association between HCMV gB genotypes and clinical outcome in the immunocompromised hosts. The goal of this study was to determine the distribution of HCMV gB genotypes and the effect of gB genotype in the developement of HCMV diseases in hematopoietic stem cell transplant (HSCT) recipients in Korea. Materials and Methods : DNA was extracted from 94 blood specimen of 52 allogeneic HSCT recipients with HCMV infection. HCMV gB genotype was determined using polymerase chain reaction to amplify a region of UL55, followed by restriction fragment length polymorphism (RFLP) analysis based on RsaI and HinfI digestion. Results : The distribution of gB types were as follows: gB1, 73.1% (38/52) of patients; gB2, 13.5% (7/52); gB3, 1.9% (1/52) and mixed infection (gB1 and gB2), 9.6% (5/52). While gB4 was not detected, a new genotype (described as gB7 by Trincado et al, 2000) was identified on the basis of their RFLP pattern. During average 708 days’ follow up period, HCMV diseases developed in 5 patients. All of them had gB1 genotype. There was no statistically significant association between the incidence of HCMV diseases and the gB genotypes. Re-infection with gB1 strain was detected in one patient who had been previously infected with gB2. This episode was associated with fever, elevated liver enzyme and positive antigenemia. Conclusion : HCMV gB1 was the dominant genotype and no gB4 was detected in allogeneic HSCT recipients in Korea, which is an unique pattern compared with the previous reports. Although we can not find significant association between the HCMV diseases and the gB genotypes, genotyping of HCMV will serve in the study of pathogenesis and transmission of this virus in transplant patients. Further study is underway with large study population.