http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jang, Ji-Yeon,Kim, Ha-Neui,Kim, Yu-Ri,Hong, Jin-Woo,Choi, Young-Whan,Choi, Yung-Hyun,Shin, Hwa-Kyoung,Choi, Byung-Tae D.A. Spandidos 2012 International journal of molecular medicine Vol.30 No.6
<P>We explored the neuroprotective effects of a hexane extract from Uncaria sinensis (HEUS) against glutamate-induced toxicity focusing on its anti-apoptotic mechanism in primary cultured cortical neurons. Pretreatment with HEUS resulted in significantly reduced glutamate-induced toxicity in a dose-dependent manner with a decrease in the release of lactate dehydrogenase. Morphological assay and flow cytometry were performed for determination of the type of cell death; according to the results, treatment with HEUS resulted in a significant reduction of glutamate-induced apoptotic cell death. We examined the anti-apoptotic mechanism of HEUS; treatment with HEUS resulted in markedly decreased expression of death receptor (DR)4, which was induced by glutamate stimulation. In contrast, treatment with HEUS resulted in significantly enhanced levels of expression of glutamate-attenuated XIAP and Bcl-2, as well as marked blockade of glutamate-induced Bid cleavage, which inhibits both extrinsic and intrinsic apoptosis pathways. In addition, pretreatment with HEUS resulted in almost complete blockade of glutamate-induced activation of caspases-8, -9 and -3, as well as cleavage of poly (ADP-ribose) polymerase. These findings suggest that the neuroprotective effects of HEUS against glutamate-induced toxicity occur via inhibition of DR4 and expression of anti-apoptotic proteins XIAP and Bcl-2 resulting in effective abrogation of the activation of caspase cascades and promotion of cell survival.</P>
김장영 ( Jang Yeong Kim ),조우리 ( Woo Ri Cho ),이진주 ( Jin Ju Lee ),( Sharav Sainzaya ),이재영 ( Jai-young Lee ) 한국폐기물자원순환학회(구 한국폐기물학회) 2020 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2020 No.-
우리나라의 원두 소비량은 약 15만 톤 수준으로 세계 6위 규모로, 2018년에는 2015년에 비해 약 21% 상승하였으며 1인당 커피소비량은 지속적으로 증가하고 있다. 그러나 커피 제조과정에서 원두의 0.2%만 사용되고99.8%가 찌꺼기로 남게 되어 대부분 폐기처분이 되는 실정으로 효과적인 처리 방안이 시급하다. 일반적으로 커피찌꺼기는 수분함량이 높고 표면이 다공성으로 높은 흡착능력을 띄는 특성을 가지고 있다. 열수가압탄화반응(Hydrothermal Carbonization, HTC)은 산소가 제한된 밀폐된 환경에서 180-300℃의 온도와 고압을 이용하여 탄화시키는 방법이며 열분해법과 달리 비교적 낮은 온도에서 진행하고 원시료 자체의 수분이 증발되어 생기는 수증기 압력을 이용하기 때문에 건조의 전처리가 필요하지 않은 비용편익적인 장점이 있다. 또한 HTC로 생산되는 biochar는 원시료와 비교 시 비표면적, 탄소함량, 발열량, 에너지 밀도가 증가하기 때문에 HTC를 이용하여 커피찌꺼기를 효과적으로 재활용하기가 적합하다고 판단된다. 따라서, 본 연구에서는 HTC를 이용하여 커피찌꺼기를 탄화시켜 biochar를 생성하는 공정을 적용하였다. 추가적으로 biochar에 화학적 활성화 과정을 통해 비표면적과 기공 등을 증가시켜 물질의 흡착력을 증가시키고 흡착속도를 개선시켜 오염물질 제거율을 높임으로써 고효율의 활성화물을 생산하였다. 이에 본 연구에서는 중금속 혼합 용액을 제조 후, Column Test를 진행하여 액상 오염물질의 흡착 성능과 암모니아 가스로 가스 흡착 Batch Test를 진행하여 대기오염물질의 흡착 성능을 평가해보고자 한다.
MiR-23b-3p Suppresses Migration, Invasion and EMT by Downregulating CD44 in Hepatocellular Carcinoma
( Na Ri Park ),( Jung Hoon Cha ),( Sung Woo Cho ),( Pil Soo Sung ),( Jeong Won Jang ),( Jong Young Choi ),( Seung Kew Yoon ),( Si Hyun Bae ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Cancer stem cells (CSC) play a key role in cancer invasion and metastasis. Among CSCs, CD44 has known as important modulators of epithelial-mesenchymal transition (EMT) together with transforming growth factor beta1 (TGF-ß1). EMT is epithelial cells lose their polarity and acquire mesenchymal cell migratory characteristics. miRNA could lead to either EMT through the regulation of various transcription factors. This study aimed to investigate the role of miR-23b-3p regulating the EMT, migration and invasion as well as CD44 expression in HCC cell lines. Methods: We induced EMT by TGF-ß1 treatment or inhibited EMT by TGF-ß1 inhibitors. Also, miR-23b-3p mimic and inhibitor were transfected into HCC cell lines. The expression of EMT-related mRNA and protein were detected by quantitative real-time PCR and western blot. Also, EMT characteristics analyzed with cell migration and invasion. Results: FACS analysis showed high expression of CD44 in two HCC cell lines with different levels of TGF-ß1 expression. TGF-ß1-negative SNU-354 cells were treated with TGF-ß1 to induce the EMT and TGF-ß1-positive SNU-368 cells were treated with TGF-ß1 inhibitor to induce the MET. The expression of miR-23b-3p was down-regulated during the EMT and up-regulated during the MET. The Inhibition of miR-23b-3p in SNU-354 cells promoted EMT, cell migration and invasion. In contrast, overexpression of miR-23b-3p in SNU-368 cells suppressed EMT, cell migration and invasion. In addition, TGF-ß1 stimulation after miR-23b-3p overexpression induced neither the mesenchymal phenotype nor cell migration. Also, CD44 is a target of miR-23b-3p. CD44 expression was increased in miR- 23b-3p inhibitor cells, whereas miR-23b-3p overexpression cells reduced expression of the CD44 in HCC cells. Conclusions: Overexpression of miR-23b-3p suppressed EMT, cell migration and invasion by targeting CD44. The results suggest that miR-23b-3p may serve as specific biomarkers and therapeutic targets for HCC.
The Optimal Management of Pleural Effusion in Liver Abscess?
( Se Ri Ryu ),( Jae Young Jang ),( Tom Ryu ),( Soung Won Jeong ),( Youngyun Cho ),( Jeeyeon Kim ),( Jeong-ju Yoo ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Young Deok 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: We investigated the characteristics and clinical features of pleural effusion in patients with liver abscess. Methods: This is a retrospective study conducted in a single center to evaluate the characteristics and management of pleural effusion in patients with liver abscess hospitalized between January 2006 and February 2018 at Soonchunhyang University Seoul Hospital. A total of 526 patients were collected. Of these, 156 patients diagnosed as eosinophilic abscess or later cholangiocarcinoma or liver metastasis were excluded from the analysis. Finally, the clinical, radiological, and laboratory findings were analyzed in total of 370 patients in liver abscess. Results: Of the patients with liver abscess, 110 (29.7%) patients had pleural effusion. Among of these, 56.4% occurred in right, 5.5% in left and 35.5% in both. When pleural effusion was present, age (older age, P=0.046), liver abscess size (43.01 vs. 59.48mm, P=0.000) and location (right superior segment, P=0.000) were significantly different from those without. Most of patients (96 patients) were treated with only antibiotics and 14 patients underwent intervention with antibiotics for pleural effusion. Of the 13 patients who were able to perform fluid analysis, 11 (84.6%) were exudate and 2 (15.4%) were transudate. Duration of pleural effusion showed no significant difference between the groups that performed intervention and those who were treated with antibiotics only (17.79 vs. 12.35 days, P= 0.112). Conclusions: The pleural effusion was more frequent in older patients, larger size and right superior segment. There was no statistically significant difference in duration of pleural effusion between intervention and non-intervention group. Therefore, treatment of pleural effusion is thought to be sufficient for antibiotics without special intervention in patients with liver abscess.