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Ock, Sangmi,Lee, Wang Soo,Kim, Hyun Min,Park, Kyu-Sang,Kim, Young-Kook,Kook, Hyun,Park, Woo Jin,Lee, Tae Jin,Abel, E.D.,Kim, Jaetaek Elsevier 2018 Biochimica et biophysica acta Vol.1864 No.4
<P><B>Abstract</B></P> <P>While deletion of <I>Akt1</I> results in a smaller heart size and <I>Akt2</I> <SUP>−/−</SUP> mice are mildly insulin resistant, <I>Akt1</I> <SUP>−/−</SUP>/<I>Akt2</I> <SUP>−/−</SUP> mice exhibit perinatal lethality, indicating a large degree of functional overlap between the isoforms of the serine/threonine kinase Akt. The present study aimed to determine the cooperative contribution of Akt1 and Akt2 on the structure and contractile function of adult hearts. To generate an inducible, cardiomyocyte-restricted Akt2 knockout (KO) model, <I>Akt2</I> <SUP>flox/flox</SUP> mice were crossed with tamoxifen-inducible MerCreMer transgenic (MCM) mice and germline <I>Akt1</I> <SUP>−/−</SUP> mice to generate the following genotypes:<I>Akt1</I> <SUP>+/+</SUP>; <I>Akt2</I> <SUP>flox/flox</SUP> (WT), <I>Akt2</I> <SUP>flox/flox</SUP>; α-MHC-MCM (<I>iAkt2</I> KO), <I>Akt1</I> <SUP>−/−</SUP>, and <I>Akt1</I> <SUP>−/−</SUP>; <I>Akt2</I> <SUP>flox/flox</SUP>; α-MHC-MCM mice (<I>Akt1</I> <SUP>−/−</SUP>/<I>iAkt2</I> KO). At 28 days after the first tamoxifen injection, <I>Akt1</I> <SUP>−/−</SUP>/<I>iAkt2</I> KO mice developed contractile dysfunction paralleling increased atrial and brain natriuretic peptide (ANP and BNP) levels, and repressed mitochondrial gene expression. Neither cardiac fibrosis nor apoptosis were detected in <I>Akt1</I> <SUP>−/−</SUP>/<I>iAkt2</I> KO hearts. To explore potential molecular mechanisms for contractile dysfunction, we investigated myocardial microstructure before the onset of heart failure. At 3 days after the first tamoxifen injection, <I>Akt1</I> <SUP>−/−</SUP>/<I>iAkt2</I> KO hearts showed decreased expression of connexin43 (Cx43) and connexin-interacting protein zonula occludens-1 (ZO-1). Furthermore, Akt1/2 silencing significantly decreased both Cx43 and ZO-1 expression in cultured neonatal rat cardiomyocytes in concert with reduced beating frequency. Akt1 and Akt2 are required to maintain cardiac contraction. Loss of Akt signaling disrupts gap junction protein, which might precipitate early contractile dysfunction prior to heart failure in the absence of myocardial remodeling, such as hypertrophy, fibrosis, or cell death.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cardiac <I>Akt1</I> <SUP>-/-</SUP>/<I>iAkt2</I> KO in mice reduces cardiac size and induces contractile dysfunction without myocardial remodeling. </LI> <LI> <I>Akt1</I> <SUP>-/-</SUP>/<I>iAkt2</I> KO hearts revealed decreased expression of Cx43 and connexin-interacting protein ZO-1. </LI> <LI> Akt isoforms may play important roles to maintain cardiac contractile function through gap junction protein stability. </LI> </UL> </P>
Studies on the Reduction of a Turkey Immunoglobulin G.
Park, Ock Jin,Choi, Tae Kyung 충남대학교 의과대학 지역사회의학연구소 1980 충남의대잡지 Vol.7 No.2
환원 및 알킬화한 칠면조 Immunoglobulin G는 gel filtration한 결과 두 개의 heavy chain(gamma chain)과 두 개의 light chain을 가진 tetramer로 나타났다. Sephadex G-200를 이용한 분자랑의 측정 결과, IgG 분자는 1.6 x 10 exp(6), heavy chain 6.9 x 10 exp(5), 그리고 light chain은 2.2 x 10 exp(5) 이었다.
Park, Kyeong Hun,Lee, Eun Suk,Jin, Yong Ik,Myung, Kyung Sun,Park, Hong Woo,Park, Chun Geon,Kong, Won Sik,Kim, Young Ock The Korean Society of Mushroom Science 2016 한국버섯학회지 Vol.14 No.4
A recent study reported that Pleurotus ostreatus has the potential to be used as a ${\beta}-glucan-based$ cream for supportive complementary therapy of atopic dermatitis. KH054 is a new herbal prescription consisting of P. ostreatus and Panax ginseng. The effects of atopic dermatitis-induced materials on the expression of cytokine genes in human monocytes (THP-1, EoL- 1) have been examined. Some reports demonstrated that P. ginseng augments the activity of natural killer cells, which plays an important role in innate immunity against infection and tumor development. Monocyte chemotactic protein 1 (MCP-1), interleukin (IL)-6, and IL-8 have important roles in mediating the infiltration of various cells into the skin of atopic dermatitis and psoriasis. The present study investigated whether KH054 on induced IL-6, IL-8, and MCP-1 secretion by house dust mite (Dermatophagoides pteronissinus) in THP-1 (human acute monocytic leukemia) and EoL-1(Human eosinophilic leukemia) cell. D. pteronissinus functions in the pathogenesis of allergic diseases, including atopic dermatitis and asthma. The inhibitory effect of KH054 on the induction of IL-6, IL-8, and MCP-1 secretion by D. pteronissinus extract in THP-1 and EoL-1 cells was examined. KH054 potently suppressed the elevated production of IL-6 and IL-8 induced by D. pteronissinus treatment in THP-1 and EoL-1 cells. Based on the present results, KH054 may be useful for developing functional foods to treat atopic dermatitis.