곰솔(Pinus thunbergii )에서 소나무재선충병 예방을 위한 나무주사용 약제선발

이상명(Sang Myeong Lee),김동수(Dong Soo Kim),이상길(Sang Gil Lee1),박남창(Nam Chang Park),이동운(Dong Woon Lee) 한국농약과학회 2009 농약과학회지 Vol.13 No.4

분기사각 덕트 내의 유동 특성에 관한 실험적 연구

이덕구,이행남,박길문 朝鮮大學校 機械技術硏究所 2001 機械技術硏究 Vol.4 No.1

The flow characteristics in dividing regions of rectangular duct system are precise, therefore their classification is very important not only in industry but also in hydrodynamics. By now, many studies of flow characteristics in dividing regions have been performed, but flow characteristics by visualizations in dividing regions have not been studied. The present Particle image velocimetry exhibits instant velocity distributions, kinetic energy distributions and vorticity, etc of the total flow fields due to the development of the accurate visualization with optical laser and optical equipment. Also, Particle image velocimetry is accurate with the flows characteristics of the dividing region as continuous analysis is done using input equipment. This study analyzes average velocity vector field, average kinetic energy and x-axis stress distribution of dividing regions with flow characteristics for visualization of Particle image velocimetry measurements in a dividing rectangular duct.

야생 등줄쥐 (Apodemus agrarius coreae) 간뇌의 Dopamine성 신경세포

정영길,이남섭,민성기,김무강 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.6

강제치환 거동특성에 관한 원심모형실험

이종호,박병수,정길수,유남재 강원대학교 산업기술연구소 2003 産業技術硏究 Vol.23 No.A

This thesis is results of centrifuge model experiments to investigate the behavior of replacement method in dredged and reclaimed ground For experimental works. centrifuge model tests were carried out to investigate the behavior of replacement method in soft clay ground. Basic soil property). tests were performed to find mechanical properties of clay soil sampled from the southern coast of Korea which was used for ground material in the centrifuge model tests Reconstituted clay ground of model was prepared by applying preconsolidation pressure in 1g condition with specially built model container. Centrifuge model tests were carried out under the artificially accelerated gravitational level of 50g Replacing material of leads having a certain degree of angularity was used and placed until the settlement of embankment of replacing material was reached to the equilibrium state. Vertical displacement of replacing material was monitored during tests. Depth and shape of replacement. especially the slope of penetrated replacing material and water contents of clay ground were measured after finishing tests Model tests of investigating the stability of embankment after backfilling were also performed to simulate the behavior of the dike treated with replacement and backfilled with sandy material. As a result of centrifuge model test. the behavior of replacement, the mechanism of the replacing material being penetrated into clay ground and depth of replacement were evaluated

등줄쥐 연수의 Tyrosine Hydroxylase, Dopamine-β-Hydroxylase 및 Phenylethanolamine-N-Methyltransferase 면역반응신경세포

정영길,이남섭,민성기 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

pV47-2 다좌위탐식자를 이용한 인체 게놈에서 다형성 유전좌위의 분리

남용석,이혜린,한길로,황적준 大韓法醫學會 1997 대한법의학회지 Vol.21 No.2

Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from λ Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)??-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)??, but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.

경사진 사석층의 지지력에 관한 원심모형실험 및 수치해석

이명욱,박병수,정길수,유남재 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.B

This paper is an experimental and numerical research works about the effects of the bearing capacity of sloped rubble mound on the density of rubble mound and the position of footing. Centrifuge model tests were performed to investigate the bearing capacity of rubble mound by changing the density of rubble mound and the location of loading in forms of strip loading to simulate the caisson. Materials of rubble mound used in the model tests were crushed rocks having similar value of uniformity coefficient to the value in field. Two different relative densities of 80% and 90% were prepared during tests. The dimens ions of centrifuge model were trapezoidal shape of model mound having the bottom width of mound, 30cm and height of mound, 10cm. Gravity level applied during the centrifuge test was 50G. Surcharge loading in the forms of strip loading was applied on the top of the sloped model mound. Tests were carried out by changing the position of loadi ng. The rigid model footing was located in the center of top of the model rubble mound and the edge of model footing was at the crest of mound. Test results were analyzed by using the limit equilibrium methods proposed by Meyer hof(1957) and Bowles(1982) and the numerical approach with FLAC being available commercially software. For the numerical estimations with FLAC, the rubble mound was simulated with the constitutive relationship of Mohr-Coulomb elasto-plastic model.

바나듐 산화막을 활용한 습도센서의 제조에 관한 연구

이승철,최복길,김남철 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

Vanadium oxides have been widely used in a variety of technological applications such electrochromic devices as infrared detectors and are expected as a material suitable for gas sensing applications. Thin films of Vanadium oxide (VOx) have been deposited by r.f magnetron sputtering under different oxygen partial pressure ratios(0%, 10%) and substrate temperatures(27℃, 400℃). Humidity-sensitive properties of resistive sensors having interdigitated electrode structure are characterized. Our sensors show good response to humidity over 20%RH to 80%RH Vanadium oxide films deposited with 0%O2 partial pressure at 400℃ exhibit greater sensitivity to humidity change than others.

90˚ 분기덕트 내의 유속 및 압력분포에 관한 연구

이행남,박길문,이종구 朝鮮大學校 機械技術硏究所 2001 機械技術硏究 Vol.4 No.2

The velocity and pressure distributions in a bifurcated duct are investigated experiment. Physical properties such as mean velocity vectors, mean vorticity and total pressure distributions are obtained for three different Reynolds numbers(578, 620, 688) using PIV measurements and CFD analysis. Also, two different dividing duct(90°) was selected for experimental study. The results of this study would be useful to the engineer in designing the flow systems for heating, ventilation, air conditioning and wastewater purification plants.

방사선 조사를 받은 흰쥐 신경뇌하수체 관문의 미세구조 : 관문의 새로운 개념설정 Establishment of a New Concept on the Barrier

이헌용,안의태,양남길,고정식,박경호 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

신경뇌하수체는 뇌의 일부지만 뇌실둘레기관(circumventricular organ)으로서 전형적인 혈액-뇌관문이 없으므로 혈관 안팎의 물질이동이 자유로운 신경내분비기관이다. 이 실험에서는 심한 방사선 조사시에 혈액-신경뇌하수체관문의 형태적 대응을-연구함으로써, 관문의 특성을 분석했으며, 관문의 구성을 구체적으로 설정하여 이를 개념화 할 것을 제안하였다. 체중 200-250g의 숫흰쥐를 sodium thiopenthal 로 마취시킨 후 Mitsubishi선형가속기로 방사선 조사를 시켰다. 조사조건은 거리 80cm, 조사구역 30 X 30cm, 조사깊이 1.2cm, 조사속도 분당 200 rads로 하여 실험군에 따라 3,000 rads 또는 6,000 rads가 되도록 했다. 조사후 6시간, 2일, 6일후에 각군의 동물을 도살 하여 신경뇌하수체를 떼어냈다. 떼어낸 조직은 1% glutaradehyde- 1% paraformaldehyde액에 1차 고정하고, 1% osmium tetroxide액에 2차 고정하였고, araldine혼합액에 포매된 조직은 절편을 만들어 uranyl acetate와 lead citrate액으로 염색해서 전자현미경으로 관찰하였다. 주로 모세혈관주위공간에서 관문구조를 중심으로 관찰한 결과 3,000 rads 조사군과 6,000 rads조사군 모두가 비슷하게 심한 변화를 보였고, 변화의 정도가 2일군에서 가장 심했으며 6일군에서는 약간 안정되는 모습이었으나 변화의 양상은 모든 실험군을 통해서 비슷하였다. 가장 두드러진 변화는 모세혈관주위공간에서 나타났는데 공간의 확장, 큰포식세포의 증가와 활성화, 신경뇌하수체세포속에 들어 있던 신경종말들의 이탈, 먼지 같은 물질의 증가 등을 들 수 있었다. 특히 큰 포식세포의 변화는 매우 특징적이어서 넓은 세포질관(판상족, lamellipoda)을 형성하여 모세혈관과 신경뇌하수체세포사이를 차단시킬 뿐아니라 왕성한 포식작용으로 공간내의 축삭종말들을 용해시켰다. 큰포식세포의 세포질안에는 과립형질내세망들이 매우 발달하였고, 세포질 영역이 매우 넓어진 것으로 볼 때, 이들이 기동타격대로서 활발히 움직이는 매우 효율적인 관문의 구성성분으로 생각되었다. 특히 신경뇌하수체는 전형적인 혈액-뇌관문이 없으므로 신경내분비기능의 수행에는 유리하나 면역활성물질이나 독성물질등 뇌에 해로운 물질의 왕래를 차단하여 제거하거나, 과다하게 분비된 신경호르몬을 조절하는 일이 중요하다고 볼 때, 큰포식세포가 이같은 역할에 적합한 구성성분이라는 결론을 얻었다. 이 실험에서는 효율성이 높은 관문으로서 혈액-신경뇌하수체관문이 구성성분을 차례로 기재하고 이를 개념화 할 것을 제안한다. 모세혈관과 축삭종말사이에 형성되어 있는 혈액-신경뇌하수체관문이 구성을 차례로 표기하면 다음과 같다 (그림 9,10). 1. 유창모세혈관 내피 2. 모세혈관 내피의 기저판 3. 혈관주위세포와 기저판("이차방어선", 한정된 범위내에서 움직일 수 있다). 4. 큰포식세포, 필요에 따라 판상족(lamellipoda)을 넓게 뻗는다.("삼차방어선", 모세혈관 주위공간 속을 이동해 다니는 "기동타격대") 5. 신경뇌하수체세포의 기저판 6. 신경뇌하수체세포의 세포질돌기(호르몬분비 조절) To study the morphological characteristics of the blood-neurohypophysis barrier system in a severely altered situation, the heads of rats were exposed to heavy X-irradiation. Rats weighing 200-250 g each were anesthetized with sodium thiopenthal, and placed on the table of Mitsubishi linear accelerator ML-4MV. Only heads of rats were placed within the exposure area of 30 cm X 30 cm. Irradiation was processed at the distance of 80 cm, with the speed of 200 rads/min in the radiation depth of 1.2cm. Total doses were 3,000 rads or 6,000 rads according to the animals of the different experimental groups. Rats were sacrificed on 6 hours, 2 days or 6 days following radiations. Tissue blocks of neurohypophyses were fixed in the 1% glutaraldehyde-1% paraformaldehyde solution, and they were refixed in the 1% osmium tetroxide solution. Ultrathin sections were contrasted with uranyl acetate and lead citrate solutions. Electron micrographs exhibited dramatic changes within the perivascular space of neurohypopysis. The perivascular space was greatly enlarged, and it contained many macrophages, floating axonal endings and plentiful flocculent materials. The enlargement was largest in the 2 day-group animals. Many macrophages are activated, and it showed tremendous cytoplasmic lamellipoda. Wide plate of macrophagic cytoplasm usually engulfed the floating axon terminals, and are located between the cappillary and the pituicytes. The situation gave the impression that macrophages prevent, filter and/or retrieve the excessive materials transported between the capillary and the axonal endings. Since the perivascular microenvironment of neurohypophysis is more vulnerable as compared with those brain areas equipped with blood-brain barrier, the functional barrier system by macrophages in the neurohypophysis should have important role. The concept of blood-neurohypophyseal barrier with the following components is proposed. The components of the barrier system from the blood pool to the hormonal pool are 1. Endothelium of fenestrated capillary 2. BAsal lamina of endothelium 3. Pericyte("second line of defense". mobile in a limited area) 4. Macrophage, extending its wide cytoplasmic plate(the lamelipoda) in need("third line of defense". freely movable in the perivascular space, "active surveillance system") 5. Basal lamina of pituicyte 6. Cytoplasmic processes of pituicyte