Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

Escherichia coli 패혈증 환자에 합병된 대칭적 하지 말단 괴사증 1예

남해성,유진홍,권순석,민준기,조현선,박민경,심병주,남유정,이지인,김진수,길욱현,조근종,신완식 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

We have encountered a rare case of symmetrical peripheral gangrene complicating Escherichia coli sepsis in a 47-years-old male. He was successfully treated with antibiotics, anticoagulants, and vasodilator. To our knowledge, this is the first report on symmetrical peripheral gangrene complicating E. coli sepsis in Korea.

A Study on Electrical Characteristics of Outdoor Termination with Viscosity of Insulating Oil

Jin‑Wook Choe,Seok‑Hyun Nam,Jin‑Gyu Kim 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.3

Outdoor terminations connecting overhead transmission lines and power cables comprise a stress relief cone, which is made of silicone rubber, and insulating oil in a composite or porcelain insulator. The lower the insulating oil viscosity, the greater is its ease of injection. However, the swelling and the degree of change in the electrical properties of the silicone rubber increase with a decrease in the insulating oil viscosity. Therefore, the efect of the expected change in the electrical proper‑ ties of the silicone rubber on the outdoor termination quality should be examined before choosing the insulating oil. In this study, specimens made of insulating and semiconducting silicone rubber were made to swell by impregnating them with three types of insulating oils with diferent viscosities. The AC breakdown strength of the swollen insulating specimens and the volume resistivity and relative permittivity of the semiconducting specimens were measured. The Weibull statistical analysis showed that the lower the insulating oil viscosity, the lower was the AC breakdown strength in overlapping con‑ fdence intervals. The volume resistivity and relative permittivity of the semiconducting specimens changed considerably with a change in the viscosity of the insulating oil. In particular, the volume resistivity of the specimen impregnated with the lowest viscosity insulating oil was 1.57×106 Ω m, indicating loss of semiconducting properties. Furthermore, the efect of changes in the electrical characteristics of the specimens on the outdoor termination quality was examined by performing an electric feld analysis. Compared with the other oils, impregnation with the lowest viscosity insulating oil resulted in the electric feld strength at the edge of the outer semiconducting layer increasing by 15.7%.

Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

외과적 노출술을 이용한 매복된 하악 제 1 대구치의 자발적 맹출 유도

김은정,김난진,조호진,김현정,김영진,남순현 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.4

하악 제 1대구치 매복의 발생 빈도는 전체 인구의 0.01%로 드물게 나타난다. 매복의 원인으로는 맹출 공간의 부족, 과잉치, 치성 종양이나 낭과 같은 맹출로상의 장애물, 유전적 요인, 내분비 질환, 외상 등이 있다. 하악 제 1대구치의 매복으로 인해 하안면 고경의 감소, 낭의 형성, 치관 주위염, 인접치의 치근 흡수, 부정 교합 등의 부작용이 생길 수 있으며, 치료 방법으로는 외과적 노출술, 교정적 견인, 외과적 재위치술, 발거 등이 있다. 다음의 두 증례는 하악 제 1대구치의 미맹출을 주소로 내원한 환아에게 외과적 노출술을 시행하여 원심 경사 매복된 하악 제 1대구치의 자발적 맹출을 유도한 증례들이다. Impaction of mandibular first molar is relatively rare and its overall frequency has been reported to be 0.01%. The etiology of impaction are lack of eruption space, physical obstacles such as supernumerary teeth, odontomas or odontogenic tumors, hereditary factors, functional disturbances of endocrine glands and traumas. Impaction of mandibular first molar can result in a short lower facial height, formation of a follicular cyst, pericoronal inflammation, resorption of the roots of neighboring teeth and malocclusion. The treatment options available for impacted teeth include surgical exposure, orthodontic forced eruption, surgical repositioning and surgical removal of unerupted molar. This report presents two cases of distally tilted and impacted mandibular first molars which were treated by surgical exposure. In these cases, we could observe spontaneous eruption of the impacted mandibular first molars after surgical exposure.

급성 관동맥증후군 환자의 신기능 이상과 주요 심장사건과의 관계

이상재,윤경호,최준호,송미진,김연경,박은미,김현정,이은미,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

EFFECTS OF LIDOCAINE ON SOMATOSENSORY EVOKED POTENTIALS IN RAT VIBRISSA/BARREL CORTEX

남순현,최병주,김현정,이만기,김영진,이혜숙 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

본 실험은 삼차신경 자극으로 발생되는 체성 감각 유발 전위에 대한 국소마취제의 효과를 관찰하였다. 나트륨 통로차단을 통하여 약리작용을 나타내는 것으로 알려져 있는 리도카인를 뇌 피질에 국소 투여한 후 삼차신경의 체성 감각유발 전위의 강도및 지연시간을 측정하였다. 케타민으로 마취된 흰쥐의 대측성 구레나룻 자극후 뇌의 체성 감각영역으로부터 기록되는 유발전위를 분석한 결과, 리도카인을 뇌 피질에 국소 투여시 유발전위의 강도 및 지연시간의 감소가 나타났으며, 필드 전위의 형태는 이상성(양극성 및 음극성) 혹은 삼상성(양극성, 음극성 및 양극성)의 파형으로 나타났다. 필드 전위의 발생 부위는 뇌 피질의 중대뇌동맥의 상행지 상방영역이었다. 본 실험에서 나타난 초기 전위변동은 피질판 상층에 존재하는 신경세포의 탈분극 과정에 의하여 생성되고 후기 전위 변동은 피질판 상층에 존재하는 신경세포의 탈분극 과정에 의하여 생성되고 후기의 전위 변동은 동일 영역의 하층 신경세포에서 과분극 혹은 재분극이 발생한 결과라고 유추된다. 따라서 삼차신경계의 체성 감각 영역에서는 피질 상층및 하층의 과립성 피라미드 세포의 순차적인 활성화에 의하여 기본적인 신경 회로망이 형성되어 있으며 생리적 자극으로 유발되는 필드 전위는 이러한 신경망를 통하여 발생될 것으로 사료된다.

악안면 성장양상에 따른 하악이부 형태에 관한 연구

남현진,유영규 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.5

교정치료에 있어서 악안면 성장의 양상은 진단과 치료계획 수립에 있어서 매우 유용한 자료이며 교합의 달성 및 안모의 형태, 발육에 많은 영향을 미친다. 따라서 성장유형의 분류에 대한 많은 연구가 있었으며 성장예측을 위한 많은 시도가 있었다. 이에 본 연구에서는 성인 120명을 대상으로 치료전 측모두부계측 방사선사진을 이용하여 전안면고경에 대한 후안면고경의 비를 측정하여 56%-62%는 시계방향 성장군(36명), 65%-80%는 반시계방향 성장군(43명)으로, 그리고 62%-65%는 정상군(41명)으로 분류하고 이에 따른 하악이부의 형태와 돌출정도 평가를 시행한 결과 다음과 같은 결과를 얻었다. 1. 남녀 하악이부 형태비교에 있어서는 남자가 여자보다 이부의 높이와 돌출정도가 크게 나타났다. 2. 시계방향 성장군이 반시계방향 성장군에 비해 하악이부의 Height, H/D ratio, Actual length는 크게 나타났고, Depth, Angle, Effective length, E/A ratio는 작은 값을 나타냈다. 3. 악안면 성장양상에 있어 이부의 돌출정도가 작을수록 시계방향성장 경향을 나타내며, 돌출정도가 클수록 반시계방향성장 경향을 나타냈다. Craniofacial growth pattern is an important diagnostic data in the course of orthodontic diagnosis and treatment planning ; it also has great influence in the establishment of occlusion as well as shaping and development of face. There have been many studies to classify different craniofacial growth patterns and attempts to predict growth patterns. This study aimed to correlate craniofacial growth pattern and symphysis morphology. 120 adult patients with age from 19 to 39 (mean age :231) were chosen as subjects ; using lateral cephalometric films their anterior to posterior facial height ratios were calculated. They were divided into 3 groups - clockwise growth pattern with 56%-62%(36subjects), counter-clockwise growth pattern group with 65%-80%(43subjects) and normal growth pattern group with 62%-65%(41subjects). Symphysis morphology and prominence evaluation in each subject were studied and the following conclusions were drawn : 1. In comparison of symphysis morphology between the sex groups, men showed large symphysis height and prominence. 2. Concerning the symphysis morphology, the clockwise growth pattern group showed larger height, H/D ratio and actual length but smaller depth, angle, effective length and E/A ratio compared to the counter - clockwise growth pattern group. 3. Those with smaller prominance of symphysis showed clockwise growth tendency and those with larger prominance showed counter-clockwise growth tendency.

여천공단지역의 유해 대기 오염물질에 대한 확률론적 위해도 분석

남병현,윤미정,이진홍 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

The study centers on a probabilistic risk assessment for the inhalation of volatile organic compounds(VOCs) and toxic metals in Yeochon industrial complex. From the assessment, the 50th∼95th percentile cancer risks by VOCs for men and women are estimated to be ??∼??, ??∼??, respectively. Those percentile risks are higher than a de minimis risk of even ??. About 4% and 1% of the 95th and 50th percentile risks for both men and women come from the only human carcinogen, benzene, while about 85% and 96% come from a probable human carcinogen, 1,3-butadiene. The 50th∼95th percentile cancer risks by toxic metals for men and women are estimated to be ??∼ ??, ??∼??, respectively. Even 95th percentile risk is lower than a de minimis risk of 10-4. About 5% and 1% of the 95th and 50th percentile risks by VOCs and toxic metals for both men and women are due to heavy metals, respectively. A comparison of the probabilistic risk estimates with point risk estimate shows that the probabilistic analysis can provide a more complete assessment of risk and more useful information to risk manager and the public. However, uncertainty remains and improved Korean probabilistic density functions(PDF) are needed for factors such as airborne concentrations, inhalation rate, exposure frequencies, and cancer potency factors.

Familial hypophosphatemia 환아의 증례보고 : A CASE REPORT

남순현,조윤정,김은정,김현정,김영진 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

가족성 저인산혈증(famiial hypophosphatemia)은 근위 세뇨관의 인산 재흡수 기전의 결핍으로 혈청 칼슘 농도는 정상이나 혈청 인산 농도가 낮아져 저인산혈증을 나타내는 가장 흔한 유전성 구루병이다. 혈청 인산이 낮음에도 불구하고, 소변으로의 인산 배출이 증가되어 있고 혈청 알칼리성 인산 분해 효소의 상승이 현저하다. 주로 반성 우성 유전이며 보통 남아에서 여아보다 임상 증상이 심하다. 이러한 가족성 저인산혈증 환자의 전신적 소견으로는 앞이마의 돌출, 사각형 머리, 작은 키, 휜 다리가 나타나며, 치과적 소견으로 치조백선과 치낭의 비박, 얇은 법랑질, 확장된 치수강과 근관, 치근단 농양과 치주 농양의 높은 발생률, 원인불명의 치근단 희박화 골염이 나타난다. 본 증례는 유치 충치 치료를 위해 내원한 환아가 임상적으로 작은 키와 휜 다리, 방사선적으로 많이 확장된 치수강과 근관이 관찰되어 소아과에 의뢰한 바 가족성 저인산혈증으로 진단받아 그 임상적 소견과 치과적 치료에 대해 보고하는 바이다. Familial hypophosphatemia is the most common hereditary rickets which occur hypophosphatemia as the calcium concentration in the blood serum is normal but the phosphate concentration in the blood serum decreases by dysfunction of renaltubular phosphorus reabsorption. In spite of the low concentration of phosphate in the blood serum discharge of phosphate by urine and alkaline phosphatase increases remarkably. It is a sex-linked and normally male show severe clinical symptoms than female. This kind of familial hypophosphatemia patients show frontal bossing. square head, short of status for general finding, and for dental finding, thinning of limina dura and dental follicle, thin and hypoplastic enamel, enlarged pulp chamber and canal, high occurance rate of periapical and periodontal abscess and unknown cause of rarefying osteitis. This case is to report about the clinical finging and dental treatment of a child patient, who came to the hospital for treatment of deciduous teeth caries but was refered to pediatrics because the child showed clinically short of status, bow-leg and radiographically enlarged pulp chamber and canal, there as diagnosed as familial hypophosphatemia.