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차미선,손홍주,이건,고명선,이상준,이종근 부산대학교 환경문제연구소 1996 環境硏究報 Vol.14 No.1
Microorganisms producing bioemulsifier were isolated from the sea water in Pusan costal aera. The isolated strain which had the highest emulsification activity and stability was identified as the genus Acinetobacter from the results of morphological, cultural and biochemical tests and named Acinetobacter sp. EL-C6 for convinience. The compositions of optimum medium for emulsification of crude oil by Acinetobacter sp. EL-C6 were crude oil 2%, NH_4NO_3 0.2%, K_2HPO_4 0.01%, MgSO_4·7H_2O 1%, CaCl_2·2H_2O 0.1%, NaCl 3% and the initial pH 7.5. The cultivation for emulsification of crude oil was carried out in 500m1 shaking flask containing 100m1 of the optimum medium at 30℃. The highest emulsification was observed after 5 days. The utilization on the various hydrocarbon of the Acinetobacter sp. EL-C6 was showed that the utilization of n-alkane compounds were better than that of aromatic compounds. Among the petroleum compounds, by Acinetobacter sp. EL-06.
김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4
Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.
Biodegradable shape-memory polymers using polycaprolactone and isosorbide based polyurethane blends
Joo, Yoon-Suk,Cha, Jae-Ryung,Gong, Myoung-Seon Elsevier 2018 Materials Science and Engineering C Vol.91 No.-
<P><B>Abstract</B></P> <P>Thermally responsive shape-memory polymers have received widespread attention in the biomedical field. In this study, biocompatible and biodegradable polyurethane (PU) and polycaprolactone (PCL) were blended to obtain shape-memory properties. Highly crystalline PCL was used as a hard segment, and PU synthesized from isosorbide, which is non-toxic and chemically and thermally stable, was used as a soft segment. The obtained PU/PCL blends containing the 30%, 50%, and 70% PU by weight were investigated for their thermal properties, mechanical properties, and shape-memory behavior. The 30%PU/PCL polymer has the best shape-memory characteristics and can be knotted by itself in the hot water bath, indicating that it can be applied in smart suture applications. The degradation test performed at 37 °C in phosphate buffered solution showed a mass loss of 2–4% for the obtained PU/PCL blends after 6 weeks. Finally, MC3T3-E1 cells cultured on PU/PCL blends showed high biocompatibility due to high adhesion and proliferation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We synthesized bio-based isosorbide-containing biodegradable PU. </LI> <LI> Thermally responsive shape-memory polymer was successfully prepared. </LI> <LI> MC3T3-E1 cells cultured on PU/PCL blends showed high biocompatibility. </LI> <LI> PU/PCL blends have potential for smart suture applications. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
한국 연근해 갯장어 , Muraenesox cinereus (FORSKAL) 의 자원관리에 관한 연구 - 5 . 자원평가 및 관리방안
손명호,강용주,장창익,박차수 한국수산자원학회 1998 한국수산자원학회지 Vol.1 No.1
Based on surplus production models using fishery data for the last 20 years, a stock assessment was conducted for the sharp-toothed eel in Korean waters. The maximum sustainable yields(MSY) from the Schaefer and Fox models were estimated to be 7,100 metric tons(mt) and 6,600 mt, respectively. An yield-per-recruit analysis showed that current yield per recruit of about 290g with F=0.255/year, where the age at first capture (tc) is 1.7 year, was lower than the maximum possible yield per recruit of 325g. Fixing tc at the current level and increased fishing intensity(F), yielded a decrease in the predicted yield - per-recruit from 290g to about 180g. However, estimated yield-per-recruit increased to 325g by increasing tc from the current age (1.7 year) to age four with F fixed at the current level. This age at first capture corresponded to the optimal length which was obtained from the F0.1 method. Yield-per-recruit and spawning biomass-per-recruit were estimated under various harvest strategies based on Fmax, F0.1, F 35% and F 40%. And also the acceptable biological catch (ABC) was estimated to be about 1,470mt. Finally, the strategies for the rational management and stock rebuilding of sharp-toothed eel were discussed and suggested, based on these evaluated values.