범게, Orithyia sinica 유생의 생존과 성장에 미치는 암모니아와 아질산의 영향

구자근,김종만,장차환,지정훈,강주찬 한국어병학회 2004 한국어병학회지 Vol.17 No.1

범게, Orithyia sinica 유생을 대상으로 암모니아와 아질산에 대한 생존 및 성장에 관한 실험을 실시하였다. 암모니아 노출 실험구에서 zoea기 유생은 노출 20일후, 대조구가 80%의 생존율을 나타내었지만 10㎎/L 이상의 실험구에서는 37-45%의 생존율을 나타내었고, megalopa기 유생에서도 유사한 경향을 나타내었다. 암모니아가 범게 유생의 성장에 미치는 영향을 조사한 결과, zoea기 유생은 20㎎/L 이상의 농도에서 megaloparl 유생은 50㎎/L 이상의 농도에서 유의한 차이를 나타내었다. (P<0.05). 아질산 노출 실험에서는 zoea기 유생과 meglaopa기 유생에서 농도와 노출기간에 비례하여 생존율이 감소하였으며, 특히 150㎎/L 이상의 아질산 농도에 노출된 zoea기 유생가 megalopa기 유생은 대조구와 비교하여 유의한 성장 지연을 나타내었다(P<0.05). This study investigates the influence of waterborne ammonia and nitrite on the zoea and megalopa stage larvae of tiger crab, Orithyiu sinicu under laboratory condition, focusing on the effects on survival and growth as deleterious responses of toxicant. Survival rate of zoea stage larvae exposed to control levels, and to 5, 10,20 and 50 ㎎/L total ammonia-N, using a continuous flow system for 20 days was 80, 77, 45, 40 and 37%, respectively. Growth rate of zoea stage larvae exposed to 20 and 50 ㎎/L total ammonia was significantly lower than in controls after 20 days (P< 0.05). Survival rate and growth rate of megalopa stage larvae exposed to ammonia also decreased at greater than 10 and 50 ㎎/L, respectively. In the nitrite exposure experiment with zoea and megalopa stage larvae of tiger crab, survival rate was decreased in a concentration and exposure period-dependent way. The growth rate of zoea and megalopa stage larvae of tiger crab exposed to nitrite decreased at greater than 150 ㎎/L nitrite concentration.

고추장 향미생산 우수 균주 선발 및 동정

구민선,김영수,오훈일,김종규 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

순창의 찹쌀고추장, 사천 밀고추장, 보은 보리고추장으로부터 분리된 숙성 시기별로 400여개 균주를 채취하여 고추장 발효용 배지에 각 균을 접종하여 30℃에서 75일간 배양 후 맛과 향에 대해 3차에 걸쳐 훈련된 관능요원들이 관능검사를 실시하고, 동시에 preparative GC를 이용하여 sniffing test를 하여 최종적으로 전통 고추장의 향과 맛에 가장 유사한 풍미를 생산하는 세균 1종과 효모 1종을 각각 선발하였다. 동정을 하기 위하여 형태학적, 생리학적 실험을 한 결과 최종 선발된 세균은 Bacillus licheniformis로, 효모는 Saccaromyces dairensis로 동정되어졌다. 특히 세균은 혐기상태에서도 성장이 가능했으며, 고추장의 주원료인 전분질 분해능이 우수했다. Microorganisms were collected to isolate the microbes producing Kochujang flavor from 3 kinds of traditional Kochujang. The strain B7 and Y20 which scored the highest sensory evaluation value among 400 microorganisms were finally selected. Also, they showed the most similar flavor pattern to the traditional kochujang's by comparison of GC chromatogram after sniffing test. As the result of the result of the morphological and physiological experiments, the bacterial strain B7 could be identified Bacillus licheniformis and the yeast strain Y20 Saccaromyces dairensis. Especially, the bacterial strain B7 could grow in anaerobic condition and was able to hydrolysis the starch that was the major component in Kochujang.

최단경로를 이용한 단순체인의 가시성에 관한 연구

구화본,엄종훈,박세명 인제대학교 1992 仁濟論叢 Vol.8 No.2

계산 기하학에서 가시성에 관한 문제는 컴퓨터 그래픽스, 로보틱스, 화상처리, CAO/CAM, 최단 경로 문제, 영역분리등의 분야에 음용이 되고 있다. 본 논문에서는 단순 다각형과 그 다각형의 단순 체인이 주어질때, 그 체인에 대해 가시성 여부를 결정하고 비가시적일때 가시성 다각형을 구하는 방법을 연구하였다. 기본 개념은 전처리 단계에서 주어진 다각형을 0(n)시간에 삼각화하고, 체인의 양끝점에서 다른 모든 절점으로 가는 최단 경로를 계산한다음, 체인의 가시성 다각형을 선형시간에 구하는 알고리즘을 제시하고 있다. In this paper, given a simple polygon and a simple chain ch(Vs,Vt), the notion of the chain visibility polygon of a simple polygon P is introduced, and an optimal algorithm for finding the chain visibility polygon in a simple polygon is presented. In the preprocessing step, the simple polygon is triangulated, and computed shortest path from Vs and Vt to all other vertices in linear time. Then, condensed dual tree composed of triangles of triangulation is traversed and visible areas from the chain is computed and merged in linear time.

비화농성 삼출성 복수를 동반하는 질환에서 복막 침생검의 진단적 의의

정종훈,서용태,오덕환,채종구,정춘해,조건국 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

Percutaneous needle biopsies of peritoneum with Abrams needle and Cope needle were done un 64 patients with non-purulent exudative ascites, who was admitted to the department of internal medicine, Chosun University hospital, from January 1978 to Deccmber 1983. The diagnostic significance and safety of the needle biopsy was estimated. The results were as follows. 1. Adequate peritoneal tissue for pathological diagnosis was obtained in 53 cases(82.8%) among 64 cases. (Abrams needle 84.8%, Cope needle 80.6%) 2. Definitive diagnosis was obtained in 31 cases among 53 cases (58.6%). (Tuberculosis 23 cases, malignancy 8 cases). 3. As compared final diagnosis with biopsy result, the rate of final diagnosis corresponding with biopsy diagnosis was 58.8% in tuberculosis and 26.3%, in malignancy, but the rate of biopsy diagnosis corresponding with final diagnosis was 100% in both tuberculosis and malignancy. 4. Cases of chronic non-specific inflammation which were diagnosed by biopsy were 13cases(24.5%)among 53 cases, and among these cases, tuberculosis was 69.2% and malignancy 30.8% on final diagnosis and among 53 cases, histologically normal was 9 cases(16.9%) of which 33.3% was tuberculosis and 66.7% was malignancy on final diagnosis. 5. Of the malignant cases on final diagnosis, Positive cytologic examination of ascitic fluid was 16.7% 6. Primary losions of cancerous peritonitis were stomach cancer (38.9%) hepatocellular cancer(22.2%) colon cancer (11.1%) ovarian cancer (11.1%) Pancreatic cancer (5.6%). 7. Complication of percutaneous peritoneal biopsy was local swelling with leakage of ascitic fluid and the accident rate were 36.4,% in Abraham needle and 3.2% in Cope needle.

반복적 스트레스가 흰쥐의 Opioid성 신경전달에 미치는 영향

사공정규,이광헌,김진성,구본훈,이종범,하정희 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연구목적: 반복적인 스트레스에 노출된 실험동물에서 opioid성 신경전달의 변화양상을 검색하고 내인성 opioid peptide의 변화양상을 알아보기 위하여 본 연구를 시행하였다. 방 법: 본 연구에서는 웅성 흰쥐에게 하루 2시간, 2주간의 반복적인 부동화 스트레스를 가한 후 뇌의 각 부위에서 opioid 수용체의 mu-, delta- 및 kappa- 아형의 최대결합력 및 친화력의 변화 양상을 관찰하였다. 결 과: 반복적 부동화 스트레스 노출군에서는 대조군에 비하여 delta-아형 수용체의 수가 시상하부(hypo-thalamus) 및 선조체(striatum)에서 증가하였다. 본 연구결과 관찰된 선조체에서의 delta-아형 수용체의 상향조절과 관련지어 delta-아형 수용체의 내인성 효현제인 enkephalin의 뇌조직 내에서의 활성도를 검색하였을 때, 반복적 스트레스 노출군에서는 대조군에 비하여 그 함량이 감소하였다. 이러한 뇌조직내 enkephalin 활성도 감소 현상은 수용체 변화와 상응하게 시상하부 및 선조체에서 관찰되었으며, 이러한 수용체의 조절 현상은 내인성 수용체 효현제인 enkephalin의 활성도의 감소에 따른 생체의 적응 현상으로 해석되었다. 결 론: 이상의 결과로부터 반복적인 스트레스는 opioid 수용체 delta-아형을 상향조절하였으며, opioid 수용체 delta-아형의 내인성 효현제의 활성도를 감소시킴을 관찰하였다. Objectives : Research into emotional or behavioral stress typically focuses upon the hypothalamic-pituitary-adrenal axis. It is well established that the hypothalamic-pituitary-adrenocortical axis is subject to inhibitory control by opioids in a variety of animal species including pigs. Exposure to acute stress induces the upregulation of opioid receptors and the release of endogenous peptides which mediate the stress-induced analgesia. There is some literature substantiating that repeated stress can lead to changes in opioidergic neurotransmission. However, the changes are highly variable. This study was designed to observe the modulatory effect of repeated immobilization stress on opioidergic neurotransmission. Methods : Male Sprague-Dawley rats weighing 150-200g were forced to suffer immobilization stress for 2 hours on each of 14 successive days. Then we examined the maximum binding capacity and affinity of each opioid subtypes(mu, delta, kappa). Results : Repeated immobilization stress increased the binding of [³H]DPDPE on the delta-subtype opioid receptor in the striatum and hypothalamus. Saturation experiments followed by scatchard analyses of the results showed an increase in the density of delta-subtype opioid receptors, but the affinity of the delta-subtype opioid receptor remained unchanged. Repeated immobilization stress reduced enkephalin activity of striatum and hypothalamus. Conclusions : From these results, it could be concluded that repeated immobilization stress up-regulated the delta-subtype opioid receptors and reduced the activity of enkephalin, an endogenous ligand for the delta-subtype opioid receptor.

주의력결핍 과잉행동장애 아동의 지적수준에 따른 정신병리와 실행기능의 차이

천은진,서완석,이종범,김진성,구본훈,송창진,성형모,배준용,배대석 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.4

To investigate psychopathoIogy and executive functions of attention deficit hyperactivity disorder (ADHD) children according to intelligence level, this study included 197 ADHD children who visited the outpatient department of neuropsychiatry of YeungNam University Medical Center, from July 2000 to June 2002. The children were divided into groups based on their intelligence levels. They were compared by the Personality Inventory for Children (PIC), Conncers' Continuous Performence Test (CPT), and Wisconsin Card Sorting Test (WCST). There were significant differences in PIC, on the subscales of verbal development, socialization and autism. In the CPT, there was no significant difference. In the WCST, there were significant differences in the total number of errors, the number of perseverative errors, the number of completed categories and the number of trials needed to complete the first category. Considering these results, the intelligence level of ADHD children is related to their disabilities and behavioral symptoms.Executive functions such as abstract thinking, categorization, working memory and flexibility had significant relationship to the intelligence levels of ADHD children. Therefore, the intelligence level of children with ADHD influences the higher executive functions of regulating attention and information processing rather than attentional functions and capacity alone.

위암종과 Epstein-Barr 바이러스와의 연관성에 대한 연구

심광용,김호영,김효열,백순구,권상옥,조미연,이창훈,이종인 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Objectives: Epstein-Barr virus (EBV) has been known to be linked to a spectrum of neoplastic conditions, including nasopharyngeal carcinoma, Burkitt's lymphoma, peripheral T-cell lymphoma and Hodgkin's disease. This study aims to investigate the association of EBV with gastric carcinoma in Korea. Methods: Fifty-three cases of gastric adenocarcinoma were studied for evidence of EBV infection by EBV-encoded small RNA (EBER) in situ hybridization (ISH) on the paraffin sections and amplifying the EBV genome encoding envelop glycoprotein (gp220) with polymerase chain reaction (PCR) in fresh gastric cancer specimens. Results: EBER was detected in 7 (13.2%) of 53 gastric carcinomas and all cases were male. In 6 (85.7%) of 7 EBER-positive cases, the tumors were located in the upper and middle part of the stomach. EBER ISH study showed strong positivity in all the tumor cells, but negativity in surrouning lymphocytes, stromal cells and normal gastric mucosa. DNA PCR was positive in 23 (43.4%) of 53 gastric carcinomas, including all of 7 EBER-positive cases. Conclusion: We could observe some association of EBV with gastric carcinoma, and our findings about the charateristics of EBV-positive gastric cancers involving sex, site and histological type are similar to those in Japan. However, EBV-positive rate of gastric cancer is higher than in Japan and lower than in Western countries. Further studies to elucidate oncogenic mechanism of EBV in gastric cancer should be performed.

랫드에서 법제유황과 비법제유황의 독성비교

김근수, 정유리, 이남진, 홍성희, 유종훈, 정재황, 강종구 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

Electronic structures of one-dimensional metal–molecule hybrid chains studied using scanning tunneling microscopy and density functional theory

Chung, Kyung-Hoon,Koo, Bon-Gil,Kim, Howon,Yoon, Jong Keon,Kim, Ji-Hoon,Kwon, Young-Kyun,Kahng, Se-Jong The Royal Society of Chemistry 2012 Physical chemistry chemical physics Vol.14 No.20

<P>The electronic structures of self-assembled hybrid chains comprising Ag atoms and organic molecules were studied using scanning tunneling microscopy (STM) and spectroscopy (STS) in parallel with density functional theory (DFT). Hybrid chains were prepared by catalytic breaking of Br–C bonds in 4,4″-dibromo-<I>p</I>-terphenyl molecules, followed by spontaneous formation of Ag–C bonds on Ag(111). An atomic model was proposed for the observed hybrid chain structures. Four electronic states were resolved using STS measurements, and strong energy dependence was observed in STM images. These results were explained using first-principles calculations based on DFT.</P> <P>Graphic Abstract</P><P>The electronic structures of metal–organic hybrid chains prepared <I>via</I> spontaneous reactions catalyzed by Ag(111) were studied using scanning tunneling microscopy/spectroscopy (STM/S) and density functional theory. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cp23295b'> </P>

섬수 ( Bufonis Venenum ) 추출물의 약리작용

김영춘(Young Hoon Kim),정성학(Seong Hak Jeong),김종호(Jong Ho Kim),최재묵(Jae Mook Choi),지준환(Joon Hwan Ji),강재구(Jae Koo Kang),박종구(Jong Koo Park),김제학(Je Hak Kim),조희재(Hi Jae Cho) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.1

N/A Bufonis Venenum is a toad venom and its main components are bufadienolides, namely resibufogenin, bufalin and cinobufagin. The desensitizing effect of Bufonis Venenum is useful for the treatment of the premature ejaculation in Chinese medicine. But, minor components of Bufonis Venenum cause problems such as topical burning, pain, and erectile dysfunction. To clarify and eliminate the components responsible for these side effects, we prepared two extracts of Bufonis Venenum with either 70% ethanol or ethylacetate and tested their pharmacological effects. The extract of Bufonis Venenum with 70% ethanol produced pain response in rat hind paw, and exhibited contraction of rabbit corpus cavernosal muscle in vitro. On the other hand, the ethylacetate extract did not cause pain and smooth muscle contraction. The desensitizing effect of the ethylacetate extract was similar to that of the 70% ethanol extract. In conclusion, these results show that the extract of Bufonis Venenum with ethylacetate does not have the components causing side effects and deserve further study for therapeutic potential in premature ejaculation in men.