H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

Modified 9Cr-1Mo 강의 항온변태처리에 따른 미세조직 및 경도변화

유정훈,주성욱,허성강,신기삼,이수,정연길 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-

Evolution of microstructure and its relation with hardness in the modified 9%Cr-1%Mo steel which was isothermally heat-treated has been investigated in various heat-treatment temperatures and time of duration. For microstructural and crystallographic analyses, optical microscope, scanning and transmission electron microscopes, energy dispersive spectrometer, and X-ray diffractometer were used. Vickers microhardness tester were used for the measurement of hardness. Precipitates of the complex M23C6, M(C,N) formed at grain boundaries and interiors of grains had led to the secondary hardening. Normalized specimen and all of the isothermally heat-treated ones showed no evidence of retained austenite.

6kW 풍력발전기의 출력곡선 측정

유능수,남윤수,이정완,조주석 강원대학교 산업기술연구소 2005 産業技術硏究 Vol.25 No.B

The power performance monitoring system for a small class of wind turbine is established. The wind turbine power performance characteristics are determined by measured power curve and the estimated annual energy production (AEP). The measured power curve is determined by collecting simultaneous measurements of wind speed and power output at the test site under varying wind conditions. In order to determine the power performance characteristics of the wind turbine accurately, the data are of sufficient quantity and quality shall be corrected according to defined criteria. In this study, the 6kW wind turbine made by Germany Inventus GmbH is examined.

9-12%Cr강의 첨가원소 및 열처리에 따른 미세조직거동과 경도변화

주성욱,유정훈,신기삼,허성강,이재현,석진익,김병훈,김정태 국립7개대학공동논문집간행위원회 2004 공업기술연구 Vol.4 No.-

9-12%Cr steel are getting increasing attention for expanded application as high temperature materials applicable for gas turbine compressors, steam turbine rotors, blade and casing. For efficient and extensive application of 9-12%Cr steel, the data in addition to the improvement of mechanical properties on the correlation of microstructural evolution and mechanical properties are inevitable and thus the investigation in this study is performed for the determination for the specimens heat-treated at 600, 650 and 700℃ for 1000, 3000 and 5000hrs. The microstructure of as-received specimen was tempered martensite, with a high dislocation density and secondary phases. Upon high temperature heat-treatment, the martensite lath got larger and dislocation density decreased resulting the reduction of the strength. In addition, sharp growth of secondary phases was observed in the specimen heat-treated at 700℃ for 5000hrs. A specimen aged at 600℃ and 650℃ Laves phase was observed. The hardness showed close relationship with the secondary phase particle and dislocation density.

수도권 일부 프랑스 레스토랑의 운영 실태 조사

고유정,이희정,주나미 숙명여자대학교 건강·생활과학연구소 2009 生活科學硏究誌 Vol.- No.26

This study is to find the revitalizing method from a survey of overall operation and characteristics of French restaurant in Seoul and Kyeonggido. Sixty-men and women of 20s took part from appointed 60 restaurants in Seoul and Kyeonggido. The statistical analysis conducts by SPSSWIN 12.0 program and the significance level is p<.05, p<.Ol, p<.OO1. The result of operation status of French restaurant is as below. Form of start-up company is popular and the period ofoperation is within 2-5 years. Mostly, restaurant is operated personally. Most of restaurants are located in residential street and apartment complex. 50-100 are most popular in weekdays and weekend customers are more than 100 people in average. So it shows meaningful differences. Most of restaurants carry on business more than 30 days without holidays and open at noon and evening in a day. Monthly rent is popular for form of possession and owners give a weight of passion and interest for applicant when hiring employees and regard management expenses as most important labor cost. Many restaurant get food staff from appointed supplier by competition. Purchase ratio of food staff compared with sales is 25%-30% mostly. 41.7% of people said the future prospect of restaurant is just ''Normal''. Most important thing of menu composition is structure and composition, particularly representative menu and gustatory quality respectively. The important thing is "Taste", sanitation, facilities, menu development, education of employee, price and etc respectively for operation of French restaurant. Sales promotions to attract more customers and relationship with around store are analyzed as less important things. The biggest difficulty is the speedy rising of prime cost for operation of frenches and restaurant, manpower shortage, decreasing customer in this sequence. The difficult for increase offoreign food service and customer complain, decrease of customers is not a big problem in operation of restaurant. This study shows differences from other studies by grasping characteristics of restaurant unlike consideration of simple French dishes and also it is incalculable value to know the overall particulars, importance and difficulties by detailed survey.

파활동경사를 고려한 소파블록 중량산정

유동훈(Yoo Dong Hoon),윤종주(Yoon Jong Joo),정평교(Jung Pyung Kyo),장문엽(Jang Moon Yup) 대한토목학회 2009 대한토목학회 학술대회 Vol.2009 No.10

인공 해수분위기에서 저탄소강의 부식특성에 미치는 합금원소 Cr, Co 및 W의 효과

심재주,유승재,최윤석,김정구,유장용 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.12

The aqueous corrosion characteristics of 1% Cr-steel alloyed with small amounts of Co and W, in synthetic seawater was studied by using immersion weight-loss tests, electrochemical corrosion tests (potentiodynamic test and electrochemical impedance spectroscopy (EIS) measurements) and analytical techniques. 1% Cr steels containing Co or W from 0.2 to 0.4 wt.% showed higher corrosion resistance than the 1% Cr steel in the immersion and potentiodynamic tests. EIS measurements showed that the Nyquist plot presented one time constant. Furthermore, the Co- and W-bearing steels present higher Rp values than the 1% Cr steel through all the test period. The better corrosion resistance of the Co-and W-bearing steels is attributed to protectiveness of the surface layer. The corrosion products were examined using electron probe microanalysis (EPMA) and X-ray photoelectron spectroscopy (XPS). The results of EPMA indicated that Cr was concentrated in the inner region of the rust layer, while Co and W were distributed all over the rust layer. XPS results showed that Co existed as a trivalent oxide in the rust layer and W in the rust appeared in the form of a WO₄ compound.

국가정신보건정책안의 우선 순위에 관한 전문가 설문조사

신영전,남정현,유원섭,이선영,유주영 漢陽大學校 精神健康硏究 2000 精神健康硏究 Vol.19 No.-

남제주 화력발전소의 증설과 저감대대책의 영향 분석

이화운,김유근,정우식,오은주,임헌호,노순아,반수진,최현정,김민선,김헌숙 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

In the view of the results investigated the effect of air pollutants that is emitted from a new generator facilities, the total estimated concentration which is the summation of a contributional concentration and observational concentration is much less than that of atmospheric environment criterion for the air pollutants. So we can predict that the operation of the new generator facilities with mitigation equipment don't seriously affect around environment. At the same time, we compared the contributional concentration of before mitigation equipment with that of after it to examine the decreasing amount due to mitigation equipment. The result showed that the concentration of SO2, NO2 and PM-10 is reduced to 25%, 30% and 26% of original value, respectively.

의무고용제도 재설계 방안

김종진,장창엽,유완식,김언아,이정주,류정진,강필수,최종철,남용현,김윤삼 한국장애인고용촉진공단 고용개발원 2008 연구개발 Vol.- No.2

현행 우리나라 의무고용제도는 장애인구의 급격한 증가, 장애인차별금지 및 권리구제 등에 관한 법률의 도입 등 외부환경변화에 신축적으로 대처하지 못하고, 경증장애인 중심으로 의무고용제도가 운영됨으로써 중증장애인의 고용배제가 우려되고 있다. 본 보고서는 이러한 문제점을 해결하기 위해 의무고용제도의 일자리 확대와 고용증가를 통해 중증장애인의 고용기회를 확대할 수 있는 정책방안을 제시하기 위한 것이다. 본 보고서는 (1)의무고용 사업체의 의무고용 산정인원에서 자립장애인 제외 (2)현행 2% 의무고용률을 3%로 상향 조정 (3)사업체가 고용하는 중증장애인에 대한 더블카운트(2배) 가산제도 도입 (4)의무고용률 미달 사업주에 대한 부담금 차등징수의 4가지 정책방안, 제도 합리화 차원에서 장려금 지급방식의 개선과 사업주의 장애인에 대한 인식개선 방안을 제시하였다. 정책방안에 대한 영향분석 결과 의무고용 적용대상자에서 비장애인과 고용상 차별되지 않는 자립장애인(6등급)을 의무고용 산정인원에서 제외하는 경우 의무고용 사업체의 고용인원은 23,296명이 감소해 고용률은 1.35%에서 0.88%로 감소하였고, 의무고용 사업체의 장애인 일자리 수는 미고용인원의 증가로 15,152개가 증가하였다. 그리고 기금은 부담금이 631억원 증가하고, 장려금이 352억원 감소해 983억원의 수지개선 효과가 발생하는 것으로 나타났다. 의무고용 사업주의 의무고용률 3% 인상 방안은 의무고용률 상향 조정에 따른 미고용인원의 증가로 장애인의 일자리 수는 43,101개가 증가하고, 기금은 부담금이 1,709억원 증가하는 반면, 장려금은 293억원이 감소해 2,002억원의 수지개선 효과가 발생하는 것으로 나타났다. 의무고용 사업주가 고용하는 중증장애인을 2인으로 더블카운트 하는 중증장애인 가산제도의 도입은 의무고용 사업체의 장애인근로자가 67,261명에서 79,685명(1,2등급만 포함시 75,685명)으로 증가하여 고용률은 1.35%에서 1.60%(1.2등급만 포함시 1.52%)로 증가하고, 의무고용률 미달 사업체의 미고용 인원이 감소하여 2,745개의 일자리가 감소하였다. 그리고 기금은 부담금이 118억원 감소하는 반면, 장려금은 418억원이 증가해 536억원의 수지악화가 발생하는 것으로 나타났다. 의무고용률 미달 사업체의 고용유인을 위해 고용률 1.0%미만, 1.0-1.5%, 1.6-2.0% 미만에 대해 각각 20%, 10%, 5%의 할증률을 순차적으로 가중하여 부과하는 부담금 차등징수 방안(3차년까지의 효과임)은 1.0% 미만 사업주는 86만원, 1.0-1.5% 사업주는 64만원, 1.6-2.0% 미만 사업주는 58만원의 부담기초액을 부과할 때 고용률은 1.35%에서 1.53%로 증가(1.6-2.0% 미만 사업주는 의무고용률 2%를 달성)하고, 미고용인원의 감소로 장애인의 일자리는 8,836개가 감소하는 것으로 나타났다. 그리고 기금은 부담금이 58억원 감소하여 수지는 악화되었다. 이상의 네 가지 방안은 모두 장애인의 고용증대를 가져온다는 측면에서는 유효하지만, 장애인의 일자리 확대를 통한 중증장애인의 고용을 증대한다는 정책목표를 달성하는 데에는 한계점을 안고 있기 때문에 어느 한 정책만을 선택하기보다는 정책효과를 극대화하기 위해 정책결합(policy mix)을 이용하는 것이 바람직함을 제시해주었다. 즉, 의무고용 적용대상자 조정방안/의무고용률 상향조정 방안과 중증장애인 가산제도/부담금 차등징수 방안을 조합 또는 모두 동시에 선택하는 경우 의무고용 사업체의 장애인 일자리 수의 확대와 중증장애인 고용을 동시에 달성하고, 기금의 수지균형도 달성 가능한 것으로 나타났다. The mandatory employment quota system of Korea does not reflect the changing external factors such as the growing population of people with disabilities(PWDs) and the enactment of the Anti-discrimination Law for People with Disabilities. The current employment quota system focuses on people with mild disabilities thus excluding people with severe disabilities from employment. The purpose of this report is to propose a plan in order to increase employment opportunity for the severely disabled through the reform of the employment quota system. This report analyzes the following : (1) exclude grade 6 disability from mandatory employment quota (2) raise the employment quota from 2% to 3% (3) implement the double counting for employers that employ the severely disabled (4) differentiate the amount of levy collected from corporations that are unable to meet the quota. The aforementioned plans are useful in that they increase employment for people with disabilities. However, there are limitation to reaching the policy objectives by increasing jobs for people with severe disabilities. Therefore, to maximize the effects we should consider policy mix of these four policies. In other words, by mixing the policies of adjusting the employment quota target/raising the quota ratio and implementing the double count/differentiating the amount of levy, we can achieve the goal of increasing jobs for the severely disabled and maintain equilibrium between incomings and outcomings.