두경부 편평세포암에서 미세혈관밀도와 경부림프절전이에 대한 연구

류준선 동국대학교 경주대학 2000 東國論集 Vol.19 No.-

Lymph node metastasis is believed to be the single most important prognostic factor in the head and neck squamous cell cancer. To identify potential biological parameters for predicting cervical lymph node metastasis, we evaluated the relationship between cervical nodal status and several parameters, such as microvessel density, p53, Ki67, and DNA ploidy, and compared with conventional clinical parameters including histologic grade and TNM staging of the tumors. This study group included 26 specimens of the primary site in patients who were diagnosed as squamous cell cancers of the head and neck. Recurred cases after chemotherapy or radiotherapy were excluded from this investigation. Analysis was performed by immunohistochemstry and DNA flowcytometry at the almost same section of the primary sites. To quantify the angiogenesis of the primary site, the immunohistochemical staining with CD-31 monoclonal antibody was performed. Microvessel density was determined by counting of CD-31 positively stained vascular endothelial cells under the magnification of x 200 field by two investigators, at an average of the largest numbers after 3 times of digitizing. Immunohistochemical staining with Ki67 and p53 were also done to evaluate the cellular proriferation of tumors and the overexpression of mutated tumor suppresor gene. DNA flowcytometry was performed to evaluate the ploidy and proliferation index. These results were compared and analyzed with clinical parameters. Any parameters failed to show significant relation to nodal status in the head and neck cancers. However, the microvessel density of the laryngeal cancers showed statistical significancy with cervical nodal metastasis. (p=0.045) Therefore, the microvessel density may have a relationship to lymph nodal metastasis in head and neck squamous cell cancer and might be used as an additional prognostic factor for planning treatment.

Neisseria elongata subsp. nitroreducens 에 의한 감염성 심내막염 1 예

원선영,정두련,주광로,이미숙,조용균,김은옥,김양수,우준희,배직현,류지소 대한화학요법학회 1998 대한화학요법학회지 Vol.16 No.2

Neisseria elongata subsp. nitroreducens는 Neisseria elongata의 세 가지 아종 중에서 인체에 병원균으로 작용하는 세균으로서 주로 심내막염을 일으키며 드물기는 하지만 그 증례 수가 점차 증 가하고 있으며 국내에서는 아직 보고된 바가 없었다. 저자들은 이전에 심질환의 병력이 없던 36세의 남자에서 치과치료 후 발생한 N. elongata subsp. nitroreducens에 의한 심내막염을 경험하여 문헌고찰과 함께 보고하는 바이다. N. elongata subsp. nitroreducens에 의한 심내막염은 경과 중 판막 파괴를 많이 초래하여 판막치환술이 필요했던 경우가 많았던 것의로 보고되고 있으나 본 증례에서는 4주간의 항균제 치료만으로 합병증 없이 호전되었다. Neisseria elongata subsp. nitroreducens differs from the other subspecies of N. elongata in additional reduction of nitrate without gas formation. Like most Neisseria, species except Neisseria meningitidis and Neisseria gonorrheae, N. elongata subsp. nitroreducens is usually classified in the group of non-pathogenin Nisseria. However, N. elongata subsp. nitroreducens, usual commensal of nasopharynx, can cause serious infections, usually endocarditis. To date, infective endocarditis with N. elongata subsp. nitroreducens has not been reported in Korea. We report a case of 36 year-old Korean male with Neisseria elongata subsp. nitroreducens endocarditis who was treated with antimicrobial agents without complication.

복귀돌연변이시험을 이용한 이온화에너지 처리 우유 및 아이스크림의 유전독성학적 평가

유지숙, 한선경, 신명수, 구본철, 최지현, 이주운, 조철훈, 함준상, 이완규 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.1

본문참고

장기 이식 환자에 있어서의 부비동염의 치료

동헌종,류준선 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.2

帶狀疱疹으로 入院한 患者 1例에 對한 症例報告

정현아,류현신,이준성,노석선 대전대학교 한방병원 2001 惠和醫學 Vol.10 No.1

From December 17th, 2001 to January 5th, 2002, the clinical study was carried out I case of patient with herpes zoster, who has been hospitalised in the Dept. of E.N.T medicine. College of Oriental Medicine, Taejon University, Taejon, Korea. The result were like below: 1. Herpes zoster virus is developed by depression of immune reaction reactivate on dorsal root ganglion. Specialy herpes zoster occured on lumbar is called Junoh-whadan in oriental medicine. 2. Herpes zoster' pathogenetic is clasifiedclasified JunGiNaSang(情志內傷), BiSilGunUn(脾失健運), QiHulUngChe(氣血凝滯). 3. Step of herpes zoster' symptom is erythema, papula, vesicle, pustule, scab. 4. Herpes zoster' pathogenetic was clasified JunGiNaSang(情志內傷) is used for Ong-Dam-Sa-Gan-Tang(龍膽瀉肝湯), incipient symptom was used venesection, UhBangJa(牛蒡子) dressing, exposure of Infra-red.

분할골절단술후 고정방법에 따른 전단강도 비교

송종운,박영준,유선열 전남대학교 치의학연구소 2001 구강과학 Vol.13 No.3

Purpose : the purpose of the present study was to compare the shear strength of the three techniques for the fixation of simulated sagittal split ramus osteotomies in terms of their ability resist vertical loads. Materials and Methods : Fifteen bovine ribs of similar size and shape were subjected to uniform osteotomies resembling a sagittal split ramus osteotomy. They were divided into three groups as wire, screw and plate group. The osteotomitized fragments were fixed with 18 gauge stainless steel wire, three bicortical screws, and with a 4-hole miniplate and monocortical screws in the wire, secrw and plate group respectively. The fixed bovine rib specimens were secured in a jig, subjected to vertical loads, and evaluated for mean shear strength at failure and for mean weight resisted by the wire, screw, and miniplate. Results : The mean weight resisted by the wire, screw and miniplate was (1.01±0.40g/㎣), (8.87±3.95g/㎣) and (6.93±2.82g/㎣) respectively. The mean weight resistances in the screw and miniplate group were significantly higher than those of the wire group (p<0.05). But no significant differences existed between the screw and plate group (p>0.05). Conclusion : These results suggest that the both screw and miniplate fixation have higher mean weight resistances than wire fixation and both methods would be much better for rigid internal fixation than wire fixation after sagittal split ramus osteotomy.

다양한 rhBMP-2 코팅 임플란트 표면에서의 인간 간엽 줄기세포의 부착, 증식, 분화반응 분석

이인구,한인호,황선욱,류재준 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

연구 목적: 본 실험은 거친 표면으로 유의성 있는 줄기세포반응을 나타냈던 4 가지의 티타늄 표면 위에 rhBMP-2를 코팅했을 때 어떤 유의한 줄기세포반응(세포부착, 증식, 분화)이 나타나는지 비교 분석함으로 rhBMP-2 코팅을 위한 가장 적절한 표면을 평가하기 위해 시행되었다. 연구 재료 및 방법: 대조군인 기계절삭표면(machined surface)과 실험군인 양극산화(anodized), RBM, SLA 표면에 rhBMP-2를 코팅한 후 코팅하지 않은 표면과 같이 8가지 표면 위에 인간줄기세포를 배양하였다. 배양 후 24시간 후SEM을 통해 줄기세포의 부착을 평가하였고 배양 3, 7, 14일후MTT와 ALP 검사를 통해 줄기세포의 증식과 분화반응을 평가하였다. 그리고 배양 7일후RT-PCR 검사를 통해 Type I collagen, osteocalcin, osteopontin의 유전자 발현의 변화를 평가하였다. 결과:SEM 평가에서 4가지 rhBMP-2 표면이 코팅하지 않은 표면에 비해 세포부착 면적이 넓고 긴밀하며 세포돌기가 더 많이 관찰되었다. 양극산화 rhBMP-2코팅표면에서 가장 두드러지게 관찰되었다. MTT 검사에서 크게 의미 있는 차이는 나타나지 않았다. ALP검사에서 양극산화 rhBMP-2코팅 표면은 대조군과 비교해서 (3, 14일) 또 RBM rhBMP-2 코팅 표면과 비교해서 (14일) 유의성 있는 ALP 활성도의 증가를 나타내었다(P<.05). RT-PCR 검사에서 osteocalcin과 osteopontin의 유전자 발현은 양극산화 rhBMP-2코팅 표면에서 높게 나타났다. 결론: 양극산화 rhBMP-2코팅표면이 줄기세포의 부착과 분화실험에서 대조군표면과 rhBMP-2를 코팅한 기계절삭표면이나 RBM 표면에 비해 유의성 있는 증가를 나타냈다(P<.05). PURPOSE. In this paper we tried to evaluate the most appropriate surface for rhBMP-2 coating among 4 rough titanium surfaces. MATERIALS AND METHODS. We used machined surface as a control group and anodized, RBM and SLA surfaces as test groups. We coated rhBMP-2 on the 4 surfaces and with uncoated surfaces for each case, we cultured human mesenchymal stem cells on all 8 surfaces. 24 hours after we measured the stem cell’attachment with SEM, and on 3rd, 7th, and 14th days, we checked the cell proliferation and differentiation by using MTT and ALP activity assay. And on the 7th day after the culture, we performed RT-PCR assay to determine whether the expression levels of Type I collagen, osteocalcin, osteopontin were changed. RESULTS. We observed with SEM that 4 rhBMP-2 coated surfaces exhibited wider and tighter cell attachment and more cell process spreading than uncoated surfaces. The anodized rhBMP-2 surface caused robustest effects. In MTT assay we could not find any meaningful difference. In ALP assay there was a significant increase (P<.05) in the ALP activity of anodized rhBMP-2 coated surface compared with that of the control (3rd and 14th days) and with that of the RBM rhBMP-2 coated surface (14th day). In RT-PCR assay there was increased expressions in the anodized rhBMP-2 coated surface for osteocalcin, and osteopontin. CONCLUSION. We found that the anodized rhBMP-2 coated surface were most prominent stem cell attachment and differentiation in compared to control and Machined rhBMP-2 coated, RBM rhBMP-2 coated surface.

알레르기성 비염 진단에서의 MAST CLA 결과

박윤근,류준선,윤창배,김기식,하경임,고병원 東國大學校 2000 東國論叢 Vol.39 No.-

Multiple allergosorbent test chemiluminescent assay (MAST CLA) is a simple method for in vitro measurement of allergen-specifie IgE antibodies. Total 125 patients was evaluated with Korean inhalant panel in diagnosis of allergic rhinitis. Overall positive rate was 53.6% and high incidence of allergen is house dust mite, house dust, cow's milk, mugwort, rye and brich. The results was slightly different from the results of large cities and the problem is absent item of domestic common trees. MAST CLA needs further improvement to be used as a primary screening test for allergy in Korea.

정상교합자와 골격성 Ⅲ급 부정교합자의 경추골성숙도에 관한 연구

양규호,최남기,최봉선,이영준,류선열,김선미 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.1

정상교합자와 골격성 Ⅲ급 부정교합자간의 성장 정도에 차이가 있는지를 평가하기 위해 8-12세 여자를 대상으로 하여 진단 석고 모형과 측모두부방사선사진을 기준으로 정상교합자 172명 , 골격성 Ⅲ급 부정교합자 191명 총 363명을 분류하여 연구를 시행하였다. 경추의 하연의 만곡도와 수직·수평 길이의 비를 측정해서 연령이 증가함에 따른 변화를 분석하여 다음과 같은 결론을 얻었다. 1. 연령이 증가함에 따라 제2~6경추의 하연의 만곡도는 정상교합자와 골격성 Ⅲ급 부정교합자 모두에서 일정하게 증가하였다. 2. 연령이 증가함에 따라 제3~6경추의 수직·수평 길이의 비도 정상교합자와 골격성 Ⅲ급 부정교합자 모두에서 일정하게 증가하였다. 3. 제2~6경추의 하연의 만곡도와 제3~6경추의 수직·수평 길이의 비에서 정상교합자와 골격성 Ⅲ급 부정교합자간의 경추골성숙도 차이를 조사한 결과 통계적으로 유의한 차이를 보이지 않았다. 이상의 결과에서 정상교합자와 골격성 Ⅲ급 부정교합자간의 경추골성숙도를 비교했을 때 유의한 차이를 보이지 않았으므로 성장에 차이가 없음을 시사하였다. This study was to evaluate and compare differences of the cervical vertebral skeletal maturity of normal occlusion and skeletal Class Ⅲ malocclusion. Normal occlusion (172 girls) and skeletal Class Ⅲ malocclusion(191 girls) were classified according to diagnosis stone model and lateral cephalogram of Korean girls aging from 8 to 12 years. The concavity of inferior border, vertico-horizontal ratio of cervical vertebrae were observed and measured according to age. Differences of the cervical vertebral skeletal maturity were evaluated. The results were as follows : 1. The concavity of inferior border of the 2nd to 6th vertebrae of normal occlusion and skeletal Class Ⅲ had uniformly increased with age. 2. The vertico-horizontal ratio of the 3rd to 6th vertebrae of girls with normal occlusion and skeletal Class Ⅲ had uniformly increased with age. 3. There was no significant difference in cervical vertebral skeletal maturity between normal occlusion and skeletal Class Ⅲ malocclusion in the concavity of inferior border of the 2nd to 6th vertebrae and in the vertico-horizontal ratio of the 3rd to 6th vertebrae. The results in the study indicate that there is no significant difference of cervical vertebral skeletal maturity between girls with normal occlusion and skeletal Class Ⅲ malocclusion.

악골에 발생한 악성 섬유성조직구종 2례

정종철,최홍란,오희균,박준아,류선열,이종호,김영운,정숭룡 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.3

Malignant fibrous histiocytoma rarely occurs in the jaws. Although the histogenesis of this tumor remains controversal, it is best regarded as a primitive and pleomorphic nature reflected by collagen production and occasional phagocytosis. It is common for metastatic foci to appear in lung and regional lymph node. There are variable treatments such as radiotherapy, surgical excision or combination, therapy of surgical excision, chemotherapy and radiotherapy. With the review of literatures, the authors report the clinical study of two cases of malignant fibrous histiocytoma of the jaw.