Surface Enhanced Laser Desorption/Ionization Time of Flight Mass Spectrometry Approach to Peptide Based Biomarker Discovery in Five Cereals: Sorghum, Foxtail millet, Hog millet, Adlay, and Corn

Jun Young Park,Yun Jung Nam,Young Ho Lee,Su Min Hwang,A Ram Kim,Tae Seok Ko,Tae Wan Kim,Sun-Hee Woo,Tae Wook Jung,Sei Joon Park 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Cereal seeds, sorghum, foxtail millet, hog millet, adlay, and corn are traditionally used as health assistant as well as energy supplying food in Korea. While beneficial phytochemicals to human have revealed in cereals, the information on peptides from cereals is far less accumulated than major reserve protein. Here, we analyzed peptide profiles using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF MS) in cereal seeds for construction of peptide information and attempted to develop peptide biomarkers for cereal identification. To optimize the analysis condition of SELDI-TOF MS, the effect of dilution factor on binding affinity to protein chips was tested using CM10 and Q10 arrays. Peptide clusters were significantly different at the level of 0.01 p-value. Peak spectra were the most stable in 1:50 of dilution factor in both chip arrays. Numbers of detected peak of 5 cereal seeds were 131 in CM10 and 74 in Q10 array. Each cereal was grouped as a cluster and well discriminated into different cluster in the level of 0.01 p-value. Numbers of potentially identified peptide biomarkers are 11, 13, 9, 5 and 12 in sorghum, foxtail millet, hog millet, adlay and corn, respectively. This study demonstrates that each cereal seed have own distinguishable specific peptides although their function are not identified yet in this study. In addition, the proteomic profiling using SELDI-TOF MS techniques could be a useful and powerful tool to discover peptide biomarker for discrimination and assess crop species, especially under 20 kDa.

습관성 유산환자에 있어서 임파구 면역요법의 임상적 연구

정환욱,오기석,전종영,강인수,최수경,황정혜,손일표,이종표,윤재범 대한산부인과학회 1993 Obstetrics & Gynecology Science Vol.36 No.11

저자들은 1990년 3월부터 1992년 12월까지 2년 10개월 동안 제일 병원 산부인과 불임크리닉에 습관성 유산을 주소로 방문한 환자 47예를 대상으로 임파구면역요법후 다음과 같은 결과를 얻었다. 1. 대상 환자는 원인 불명의 습관성 유산 환자가 35예이었으며, 조직 적합성 항원검사(HLA typing) 및 차단항체 검사상 동종면역학적인 원인에 의한 습관성 유산 환자가 12예였다. 이중 일차성 및 이차성 습관성 유산 환자가 각각 44예와 3예였다. 평균 연령은 32.1세, 평균 자연유산 횟수는 3.5 회였다. 2. 임파구 면역요법후 임상적인 결과는, 대상 환자 총 47예중 31예에서 임신이 되었고, 27예에서 임신이 성공적으로 유지되었으며, 4예에서는 다시 유산되었다. 3. 면역요법후 임신 성공률을 보면, 임신된 31예중 27예에서 임신이 성공적으로 유지되어 87%였 다. 4. 면역요법후 임신까지의 기간을 보면, 평균 6.7주였으며 8주이내에 임신된 경우가 16예로 가장 많았고, 8주 이후 임신된 경우가 8예 있었으며, 임신중에 면역요법을 실시한 경우도 7예 있었다. 5. 면역요법후 다시 유산된 원인을 보면, 원인을 알수 업는 경우, 임신 20주에 조기 양막 파수와 쌍태아 임신으로 유산된 경우 유산아의 염색체 검사상 47,XX,+7인 경우 및 차단항체의 부족이 유 산의 원인으로 생각되는 경우가 각각 1예씩 있었다. 6. 면역요법후 분만된 21예에서 분만시 임신주수는 평균 39.4주였으며, 출생시 신생아의 체중은 평 균 3,315gm이었다. 면역요법후 태아 측의 합병증으로 생길 수 있는 조산 및 자궁내 태아 발육지연 은 각각 1예(4.8%)와 2예(9.5%)있었다. 결론적으로 면역요법 대상 환자를 정할 때는 습관성 유산의 다른 원인에 대한 기본적인 검사를 철 저히 해야 하고(Table 1), 원인 불명이거, 조직 적합성 항원검사, 혼합 임파구 배양검사 및 임파구 독성 항체검사에서, 동종면역학적인 원인에 의한 것으로 진단된 습관성 유산 환자의 경우 임파구 면역요법은 효과적으로 안전한 것으로 생각된다. 47 patients with unexplained (n=35) or alloimmunollogic (n=12) recurrent abortion were studied for the evaluation of the clinical results after lymphocyte immunization The results were as follows; 1. The mean age of these patients was 32.1 years okd and the mean number of spontaneous abortion was3.5 2. Among 47 immunized women 31 became pregnant, 27 had successful pregnancies, and four had repeated abortions 3. Thus, the seccess rate after lymphocyte immunization was 87%(27/31) 4. The mean interval from immunization to pregnancy was 6.7 weeks, 16 became pregnant before eight weeks, eight had after eight weeks, and seven were immunized in early pregnancy 5. The outcomes of immunotherapy in 21 delivered women were such that the mean gestational weeks was 39.4 and the mean birth weight of neconates was 3,315gm The fetal complications of immunotherapy in this study were one preterm delivery(4.8%)and two intrauterine growth retardation(9.55) In conclusion, our study shows that immunization with paternal cells remains a safe and effective treatment for the majority of women with unexhlained or alloimmunolohic recurrent spontaneous abortion, resulting in developmentally nromal babies with normal birth weight.

질식초음파를 이용한 자궁벽을 통한 태반 융모막 융모 채취법 ; 경질적 경근적 융모막 융모 채취법

정환욱,오기석,강인수,최수경,황정혜,이종표,김재영,전종용 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.1

1991년 1월 1일부터 1992년 6월 30일까지 본원에 내원한 환자중 산전유전질환의 진단이 필요한 산모 87예를 대상으로, 질식초음파 감시하에 천자침으로 자궁벽을 통하여 융모막 융모 생검으 실시한 결과 다음과 같은 성적을 얻었다. 1. 대상 87예의 적응증증 모성연령증가가 50예(57.7%)로 가장 많았으며, 평균 연령은 35세였고, 평균 임신주수는 10주였다. 2. 천자침의 삽입횟수는 평균 1.2회였고, 3회이상 삽입하지는 않았다. 3. 융모막 융모 채취성공율은 98.8%로, 총대상 87예중 86예에서 채취가 가능하였고, 80명에서 1회 천자로 채취가 가능하여 91.9%의 성공율을 보였다. 4. 채취된 융모양은 5-20 mg 으로 평균 14 mg이었고, 대상 모두에서 검사하기에 충분한 양이었다. 5. 염색체이상 발생빈도는 8%로 7예에서 염색체 이상을 보였다. 6. 합병증으로는 융모막하혈종이 13.8%로 발생하였으나, 대부분 1주후 소실되었고, 기타 질출혈 2회, 자연유산 1예였으나 감염 및 양수과소증 등의 다른 합병증은 없었다. 결론적으로 질식초음파를 이용한 융모막 융모채취법은 감염의 위험성이 적고 실시하기에 용이하며 안전하므로 기존의 고식적인 자궁경부를 통하거나 복벽을 통과하는 방법보다 유용한 방법으로 사료된다. Data are presented on 87 patients undergoing chorionic villus sampling procedures by new sampling technique: Transvaginal transmural catheter aspiration method undertransvaginal ultrasound guided at Cheil General Hospital from January, 1991 to June 1992. Eighty seven pregnant women underwent Transvaginal-Transmural Chorionic villus sampling(TM-CVS) between 8 and 10 weeks of gestation. Sampling was usually carried out at ninth or tenth weeks of gestation (56 cases, 64.3%). Transmural chorionic villus sampling had an overall success rate of 98.8%, with only one insertion of the guide needle required for 91.9% of patients. Analysis of the 87 samples revealed seven anomalities *8%). The spontaneous abortion rate for these method was 1.1% (1.87). Of 87 attemped cases, subchorionic hematoma occured in 12 cases (13.9%) and vaginal bleeding in 2 cases. These results demonstrate that the transvaginal transmural procedure can be rapidly and effectively incorporated by and operator already experienced with transcervical and transabdominal chorionic villus sampling.

블랙아이스 제거를 위한 탄소나노튜브 기반 도로 코팅제 개발 및 성능평가

이승준 ( Lee Seung-jun ),정유진 ( Jung Yu-jin ),장승환 ( Jang Sung-hw An ) 한국구조물진단유지관리공학회 2021 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.25 No.2

블랙아이스는 겨울철 도로의 검은 아스팔트 표면에 코팅한 것처럼 얇은 얼음 막이 생기는 현상이다. 이로 인한 사고의 위험성이 대두되고 있으며, 이는 겨울철 운전자뿐만 아니라 구조물에 까지 피해가 발생하며 최근 구조물 유지관리에 위협적인 요인으로 인식되고 있다. 기존의 도로 결빙 제거 방식으로 염화나트륨을 이용하지만 이는 구조물 및 차량 부식으로 인해 또 다른 문제를 야기하고 있다. 본 연구에서는 기존 도로 코팅제를 탄소나노튜브를 이용하여 전도성 물질로 성능을 변화시켜 줄히팅을 이용한 코팅 자체 발열 기능을 가진 다기능성 도로 코팅제를 개발하였다.

Functional MR Imaging of the Alar Ligament : Morphologic Changes during Axial Rotation in Asymptomatic Adults 1

KH , Choi,YH , Choi,MK, Yoon,HW, Jung,SJ , Oh,BY, Jun,HJ , KIM 대한자기공명기술학회 2000 대한자기공명기술학회지 Vol.10 No.-

Impacting behavior of bulk metallic glass powder at an abnormally high strain rate during kinetic spraying

Yoon, Sanghoon,Lee, Changhee,Choi, Hanshin,Kim, Hwí,jun,Bae, Jungchan Elsevier 2007 Materials science & engineering. properties, micro Vol.449 No.-

<P><B>Abstract</B></P><P>Impacting behavior and deposition formation of Ni–Ti–Zr–Si–Sn bulk metallic glass during kinetic spraying were investigated in this study. Bulk metallic glass particle deposition was primarily dependent on the impacting particle velocity. Secondly, the impacting particle temperature could transfer the deposition mode from fracture-induced local melting to severe viscous flow, especially for the splat on previously deposited splat. It is suggested that the deposition behavior of bulk metallic glass particles is determined by the competition between fracture and deformation. Fracture and deformation are controlled by adiabatic shear instability at the faying interface during impacting, with causal factors including temperature-dependent superplasticity of the bulk metallic glass and an abnormally high strain rate of the individual particle impact during kinetic spraying.</P>

연구노트 : 3T3-L1 지방세포내 ROS 생성에 대한 천년초 열수 및 에탄올 추출물의 항산화 효과

윤보라 ( Bo Ra Yoon ),이영준 ( Young Jun Lee ),김선구 ( Sun Gu Kim ),장중영 ( Jung Young Jang ),이효구 ( Hyo Ku Lee ),이성갑 ( Seong Kap Rhee ),홍희도 ( Hee Do Hong ),최현선 ( Hyeon Son Choi ),이부용 ( Bo Yong Lee ),이옥환 ( Ok Hw 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2012 한국식품저장유통학회지 Vol.19 No.3

Recently, NADPH oxidase 4 (NOX4)-mediated generation of intracellular reactive oxygen species (ROS) was proposed to accelerate adipogenesis of 3T3-L1 cell. We have previously shown that Cheonnyuncho (Opuntia humifusa) extract significantly inhibited adipocyte differentiation via downregulation of PPARγ (peroxisome proliferator-activated receptor gamma) gene expression. In this study, we focused on the molecular mechanism(s) of NOX4, G6PDH (glucose-6-phosphate dehydrogenase) and antioxidant enzymes in anti-oxidative activities of 3T3-L1 adipocytes. Our results indicate that Cheonnyuncho extracts markedly inhibits ROS production during adipogenesis in 3T3-L1 cells. Cheonnyuncho extracts suppressed the mRNA expression of the pro-oxidant enzyme such as NOX4 and the NADPH-producing G6PDH enzyme. In addition, treatment with Cheonnyuncho extract was found to upregulate mRNA levels of antioxidant enzymes such as Mn-SOD (manganese-superoxide dismutase), Cu/Zn-SOD (copper/zinc-SOD), glutathione peroxidase (GPx), glutathion reductase (GR), and catalase, all of which are important for endogenous antioxidant responses. These data suggest that Cheonnyuncho extract may be effective in preventing the rise of oxidative stress during adipocyte differentiation through mechanism(s) that involves direct down regulation of NOX4 and G6PDH gene expression or via upregulation of endogenous antioxidant responses.

Validation of the Risk Prediction Scores for Hepatocellular Carcinoma in Patients with Chronic Hepatitis B Treated with Entecavir or Tenofovir

( Jin Won Chang ),( Jae Seung Lee ),( Hye Won Lee ),( Beom Kyung Kim ),( Jun Yong Park ),( Do Young Kim ),( Sang Hoon Ahn ),( Yeon Seok Seo ),( Han Ah Lee ),( Mi Na Kim ),( Yu Rim Lee ),( Seong Gyu Hw 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Several prediction scores are available for the early detection of hepatocellular carcinoma (HCC). We validated the predictive accuracy of the AASL, RESCUE-B, PAGE-B, and modified PAGE-B (mPAGE-B) scores in patients with chronic hepatitis B (CHB) treated with entecavir (ETV) or tenofovir disoproxil fumarate (TDF). Methods: Between 2007 and 2014, 3,171 patients were recruited (1,645 with ETV and 1,517 with TDF). The predictive accuracy of each prediction score was assessed. Results: The mean age of the study population (1,977 men and 1,194 women) was 48.8 years. Liver cirrhosis was noted in 1,040 (32.8%) patients. During follow-up (median, 58.2 months), 280 (8.8%) patients developed HCC and were significantly older; were more likely to be male; had significantly higher proportions of liver cirrhosis, hypertension, and diabetes; and had significantly higher values of the four risk scores than those who did not develop HCC (all P<0.05). Older age (hazard ratio [HR]=1.048), male sex (HR=2.142), liver cirrhosis (HR=3.144), and prolonged prothrombin time (HR=2.589) were independently associated with an increased risk of HCC development (all P<0.05), whereas a higher platelet count (HR=0.996) was independently associated with a decreased risk (P<0.05). The predictive accuracy of the AASL score was highest at 3 and 5 years HCC prediction (area under the curve [AUC]=0.818 and 0.816, respectively), followed by RESCUE-B, PAGE-B, and mPAGE-B scores (AUC=0.780-0.815 and 0.769-0.814, respectively). Conclusions: Four HCC prediction scores performed acceptably in Korean patients with CHB treated with ETV or TDF. Of these, the AASL score showed the highest predictive accuracy.

동맥색전술로 시술한 폐격리증

이건화 ( Gun Wha Lee ),김동균 ( Dong Kyun Kim ),나현식 ( Hyun Sik Na ),배준용 ( Jun Yong Bae ),최준호 ( Jun Ho Choi ),김양기 ( Yang Ki Kim ),김기업 ( Ki Up Kim ),어수택 ( Soo Taek Uh ),김동훈 ( Dong Hoon Kim ),황정화 ( Jung Hwa Hw 대한결핵 및 호흡기학회 2005 Tuberculosis and Respiratory Diseases Vol.58 No.1

Pulmonary sequestration is a very rare congenital malformation in which a mass of pulmonary tissue is detached from the normal lung and receives its blood supply from a systemic artery. It may be clinically asymptomatic or it has a wide spectrum of variou

말초혈액을 이용한 초고속 유전독성평가법 개발 연구

안일영 ( Il Young Ahn ),김주환 ( Ju Hwan Kim ),임대현 ( Dae Hyun Lim ),양준영 ( Jun Young Yang ),김소영 ( So Young Kim ),이정선 ( Jung Sun Yi ),염영나 ( Young Na Yum ),임채형 ( Chae Hyung Lim ),이진하 ( Jin Ha Lee ),최기환 ( Ki Hw 한국동물실험대체법학회 2014 동물실험대체법학회지 Vol.8 No.1

To identify mutagenic potential of test substances, in vitro Ames tests are commonly used. Recently revised ICH S2(R1) guideline requires in vivo genotoxicity test if the result of the in vitro test is positive. In addition, a method testing multiple endpoints is required for animal welfare. Therefore we established a flow cytometry-based analysis such as Pig-a gene mutation assay and the micronuclei assay for detection of in vivo genotoxic potential using peripheral blood collected from repeated dose toxicity study. To evaluate these new methods, male Sprague Dawley rats were treated for 3, 14 or 28 days with N-nitro-N-ethylurea (ENU). ENU induced mutations in both reticulocytes (RET) and red blood cells of rats dose-dependently from the Pig-a gene mutation assay. ENU also increased micronucleated reticulocytes frequencies in flow cytometry based micronuclei assay, implying chromosomal damage to hematopoetic cells. These data show that both assays were well established. We additionally evaluated urethane and glycidol for applicability of Pig-a gene mutation assay and in vivo micronuclei assay by flow cytometry. Urethane, compared with vehicle control, did not increase Pig-a gene mutation and micronuclei frequency. Glycidol, compared with vehicle control, did not increase in micronuclei frequency, but Pig-a gene mutation significantly increased in the highest concentration for 28 days. Pig-a gene mutation assay for genotoxicity has many advantages: It can detect mutation in various species including humans, primates and rodents; and is integrated with repeated dose toxicity test without additional usage of animals; and has low spontaneous mutation frequency.