Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

박목월의 시 <나그네>의 예술가곡화 연구

이지연 ( Lee Ji-yeon ),김용범 ( Kim Yong-bum ) 한민족문화학회 2016 한민족문화연구 Vol.56 No.-

본 논문은 박목월의 시 <나그네>의 예술가곡화에 대한 연구이다. 예술가곡이란 18세기 독일어권에서 완성된 음악 양식으로, 시인의 시와 작곡가의 작곡 그리고 성악가에 의한 가창으로 표현되는 예술로 문학과 음악이라는 두 가지의 예술 장르가 합일된 문화융합의 산물이다. 이러한 예술가곡이 우리나라에 도입정착 된 후 1920년에 우리나라 최초의 가곡 홍난파의 <봉선화>가 작곡되지만 한국예술가곡의 효시 작품에 대한 논란이 존재하였고 독일 예술가곡의 개념과 정의 및 조건에 입각한 필요충분조건을 모두 갖춘 한국최초의 예술가곡으로는 정지용 작시, 채동선 작곡의 <고향>(1932)으로 비정하고 있다. 특히 1920년대는 음악이나 시문학이 아직은 확고하게 예술장르로 자리 잡지 못한 시기였음으로 시인과 작곡가가 협업을 통해 창작해야하는 예술가곡의 시기로서는 상호 불완전했었다 할 수 있었고 한국문학은 1930년대에 이르러 시인들이 자신들의 창작 행위에 대한 뚜렷한 자의식을 갖게 된다. 그 중 본고가 주목하는 것은 한국 시문학사의 독보적 위상을 차지하고 있는 시인 박목월과 그의 시를 가사로 한 예술가곡들이다. 박목읠의 초기시가 가지고 있는 서정성과 율성 즉 음악성은 작곡가들에게 주목받아 김연준을 비롯하여, 김성태, 김순애, 이수인, 윤이상 등에 의해 많은 예술가곡이 탄생하였는데 예술가곡 작품들을 일별하는 과정에서 유독 <나그네>를 대상으로 작곡된 작품이 많았음을 발견하고 음악적, 문화융합론적, 언어기호학적 관점에서 그 이유를 분석 및 규명해보았다. 시의 정형리듬을 형성하려는 계산에서 이루어진(artificial const-ruction)박목월의 <나그네>는 예술가곡의 작곡에서 조성, 박자, 악절과 결합하기에 최적의 조건이었고 두드러진 유음의 사용은 성악의 발성을 용이하게 하는 요소로 작용된다. 따라서 시인의 시와 작곡가의 작곡뿐 아니라 예술가곡의 필요충분조건의 하나인 노래 불려 져야 한다는 가창의 조건으로 볼 때 나그네가 가지는 예술가곡의 적합성은 예술가곡성립의 필요충분조건과 자질을 모두 갖추고 있음을 알 수 있다. 한 시인의 특정한 작품에 14명의 작곡가가 곡을 붙였다는 것은 우리 예술가곡사 상 특이한 사례에 속하며 이는 박목월 <나그네>의 문학적 가치를 넘어선 문화융합의 새로운 가치를 의미하는 것이다. This study aims to examine the capability of Mok-wol Park`s poetry, "Naguenae", as an art song. The art song, originally developed in Germany in the eighteenth-century, is a genre of art that unites poetry and music; the composers put a melody on poems so that singers could sing the songs. As an outcome of cultural convergence, the two independent forms of art, literature and music, came to converge into a completely single genre, heralding a birth of a new musical style. Korean poetry began to mature in the 1930s and Mok-wol Park, the most representative poet throughout the history of Korean literature, shows lyrical and rhythmical features on his early poems. This musicality was valuable to be used as text for art songs by many contemporary composers such as Yeon-jun Kim, Sung-tae Kim, Sun-ae Kim, Soo-in Lee, and I-sang Yoon. In the process of categorizing their art songs, we discovered that there are numerous songs using particularly "Naguenae" as a text. This study mainly explores and analyzes reasons from musical and glossematic perspectives, as well as from viewpoint of the theory of cultural convergence. Mok-wol`s "Naguenae" written based on a calculation of its regular rhythmic patterns has the optimum condition as a text for the musical setting in which a tonal center, rhythm, and phrasing can be combined without any disturbances. Moreover, his use of liquid sound aids a singer`s vocalization. Therefore, in view of the fact that a song is to be sung, which is a necessary and sufficient condition for an art song, together with a text and musical setting, "Naguenae" qualifies all conditions. The fact that one particular poem written by one poet was used as a text for an art song by fourteen different composers is unusual case in Korean history of art songs. In addition, Mok-wal Park`s "Naguenae" is not only literary valuable, but means a new step of cultural convergence.

가평 전주이씨묘 출토복식 고찰

최연우,박윤미,김윤경,김지희,박양희,이선우,차서연,편나영,황혜남,Choi, Yeon Woo,Park, Yoon Mee,Kim, Yoon Gyung,Kim, Ji Hee,Park, Yang Hee,Lee, Seon U,Cha, Seo Yeon,Pyeon, Na Young,Hwang, Hye Nam 한국의류학회 2017 한국의류학회지 Vol.41 No.5

In 1995, the costumes were restored in an unknown woman's tomb at the Jeonju Lee's family cemetery in Gapyeong, Gyeonggi-Do, and there are currently nine items remaining. In this study, we first introduced these 9 relics to academia and analyzed the morphological characteristics of the costumes. We also estimated the time and person of burial compared with costumes unearthed from other burials. Jeogories (short jacket) are all four items. One of these items was unusual in shape, and the upper part of the seop (gusset) was wrinkled and shaped. This type of Jeogori appears only till the 1520s in other tombs. There are also two skirts, one of which is characterized by a superimposed pattern. The top and bottom / middle part of the skirt were rolled up, and the skirt, which was double-rolled up in this way, was first discovered. An analysis of the shape of Jeogori and skirt indicated that the person buried in the Jeonju Lee's family tomb was believed to have survived until the early 16th century. Based on these estimated periods, as a result of looking at the genealogy of Jeonju Lee's family, it was concluded that the tomb was probably a woman named Lee Geum Myeong (李金命) born in the mid-15C and died between the late 15C and the early 16C.

활동이론의 관점에서 바라본 박물관, 커뮤니케이션, 그리고 학습

박지연,이수민,이병준 한국문화교육학회 2011 문화예술교육연구 Vol.6 No.4

Museum is a medium which communicates with previous generation's experiential memories and present generation's cultural memories. Therefore, the communication is a core task in museum. Museum needs mediums like exhibition and education and we have to discuss how to structuralize these things. The museum communication could be explained through the Cultural-Historical Activity Theory(CHAT). The CHAT has been developed by several theorists like Vygotsky. Especially, the third generation's CHAT has been developing by Engeström. It is a worthy theoretical structure that can show museum as a messenger of communication in society and its communication problems. This study is based on the CHAT and analyzes museum in a social activity system and activity system in a museum from micro/macro-scopic view. Also, it produces practical models that connect museum with community art field and museum with schools. 박물관은 앞선 세대의 체험적 기억을 현 세대에게 문화적 기억의 형태로 커뮤니케이션하는 매개체이다. 따라서 박물관에서 소통(communication)이라는 개념은 핵심적인 과제일수 밖에 없다. 박물관은 그 자체로 전시와 교육과 같은 매체(medium)를 필요로 하며 이를 어떻게 구조화할 것인가의 문제가 박물관커뮤니케이션의 측면에서 논의될 필요가 있다. 이러한 거시적, 미시적 차원의 박물관커뮤니케이션의 과제는 비고츠키와 여러 이론가들의 연구성과를 정리하고 발전시킨 활동이론에 의해 잘 설명될 수 있을 것으로 보인다. 특히 Y. Engeström에 의해 발전된 제3세대 활동이론은 사회속에서의 커뮤니케이션의 전달자로서의 박물관과 박물관내에서의 커뮤니케이션의 문제를 잘 드러낼 수 있는 이론체계라고 생각된다. 본 연구는 활동이론에 근거하여 거시적·미시적 관점에서 사회활동시스템 속에서의 박물관과 박물관 내에서의 활동시스템을 분석하고 이를 통해 박물관의 커뮤니케이션을 박물관 내부 활동시스템 간의 네트워크 실천, 박물관과 지역의 예술장(藝術場)이 연계된 네트워크 실천과 박물관과 학교가 연계된 네트워크 실천 모형을 구조화하여 제시하려 한다.

신경모세포종의 화학 및 방사선요법이 치아우식활성도에 미치는 영향

이지현,박기태,성기웅,김지연 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.3

소아암 환자의 치료 방법으로 주로 사용되는 항암화학요법이나 방사선요법이 구강 건강에 미치는 영향은 매우 크다. 구강 점막염, 타액 분비율의 변화, 구강내 감염을 야기할 수 있고, 부분무치증, 왜소치, 치근 발육이상, 저석회화증이 나타날 수 있다. 본 연구는 신경모세포종으로 치료 받은 환아들에 있어 항암치료가 치아우식증 발생 위험도에 어떠한 영향을 미치는지 알아보기 위해 실시되었다. 삼성서울병원 소아청소년과에 신경모세포종으로 내원하여 화학 및 방사선치료요법을 받은 환아들을 대상으로 Dentocult.. SM, Dentobuff.. Strip을 이용하여 항암치료 후의 타액 분비율, 타액 완충능, 타액 내의 Streptococcus mutans 수를 알아보고 정상 어린이들의 결과와 비교하였으며, 신경모세포종 환아에서 처음 치료를 시작한 나이, 치료 종결 후 경과 시간에 따라 치아우식활성도가 어떻게 달라지는지에 대해서도 알아보았다. 그 결과 타액 분비율은 환아가 대조군에 비해 유의성 있게 낮은 결과를 보인 반면(p<0.05), 타액 완충능과 Streptococcus mutans 수는 환아와 대조군 간에 유의성 있는 차이를 보이지 않았다. 환아에서 치료를 시작한 나이나 치료 종결 후 경과 시간에 따른 치아우식활성도에서도 통계적 유의차는 없었다. Chemotherapy or radiotherapy used for the treatment of pediatric cancer may have many adverse effects on the oral cavity. Oral mucositis, reduced salivary flow, oral infection, hypodontia, microdontia, arrested root development, and enamel hypoplasia are common oral complications. The aim of this study is to evaluate the effects of cancer therapy on dental caries activities. The children who had been treated for neuroblastoma in the department of pediatrics, Samsung Medical Center, were included and healthy children served as controls. The salivary flow rate, salivary buffering capacity, and Streptococcus mutans counts of both groups were evaluated using Dentocult SM and Dentobuff Strip. The dental caries activity related to the age at the start of treatment and the time elapsed since treatment completion were also evaluated. As a result, neuroblastoma patients had significantly lower salivary flow rate than the controls, while there were no significant differences between two groups as for salivary buffering capacity and Streptococcus mutans counts. The dental caries activities related to the age at the start of treatment and the time elapsed since treatment completion were not significantly different.

경부외측종괴의 감별진단 및 접근에 대한 고찰

이경호,박현진,강지연,최희원,조용석,김경원,양수남 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.6

Most neck masses of specific cause occur in rather predictable locations within typical age groups. This allows a systematic approach to developing a working diagnosis and a differential diagnosis and management plan for the patient presenting with a neck mass. Pathologic masses of the neck will be divided into the following segment: 1. Masses of nonspecific location. 2. Masses in the submandibular region, 3. Masses in the parotid region, 4. Masses in the median-paramedian region, 5. Masses in the lateral neck region. The lateral neck region is the area posterior to the hyolaryngotracheal conduit, below the posterior belly of the digastric muscle and tip of the parotid gland, and extends down to the clavicle. The contents of this region include the large vessels, nerves and greatest number of lymph nodes of the neck, and lymph node metastasis in this region is usually from a primary lesion at the base of the tongue or elsewhere in the oropharynx. Common lateral neck masses include branchial cleft cyst, carotid body tumor, neurogenic tumor, pan-neck infection, cystic hygroma, hemangioma, lipoma and lymphoma. Especially lymphosarcoma. Hodgkin's disease, cystic hygroma, hemangioma and branchial cleft cyst and more common and often manifest in the young patients. Many head and neck disease processes manifest as neck masses. These conditions are treated by surgical excision, except for some inflammatory masses, and often those also must be excised before a diagnosis can be made. We retrospectively reviewed 5-year experiences in the treatment of lateral neck masses at our department to define better clinical characteristics of the masses and the optimal diagnostic and therapeutic approaches to these rare diseases.

보육교사의 아동학대에 대한 인식 및 발견경험에 대한 연구

이재연,김지윤 한국영유아보육학회 2002 한국영유아보육학 Vol.0 No.28

The purpose of this study was to find the preschool teacher's awareness on child abuse which is defined by physical abuse, neglect, emotional abuse and sexual abuse. Also, it was to find the preschool teacher's awareness on child abuse report. The results of this study were as follows: First of all, the preschool teachers' awareness on child abuse showed a resonable low-points to all abuse patterns. Especially, the preschool teachers' awareness is lower the behavior syndrom than the physical syndrom on abuse. The next, for one year ago, experience which found out abused child was 56 of 284 teachers. And preschool teachers' awareness on the needs of the report center and report system showed mostly(97.1%) that need. For the last time, the awareness on the education experience of child abuse showed that 50% of the preschool teachers educated properly. Many of the preschool teachers(96.8%) showed that they had a great deal of education experience that they could find out child abuse during teaching. Also, many of the teachers(94.8%) showed that participate in education.

(불)가능한 증언과 재현의 젠더 : 손소희 소설에 나타난 ‘죽음’의 초점화를 중심으로

이지연 이화어문학회 2021 이화어문논집 Vol.53 No.-

The purpose of this paper is to refute the criticism that it is difficult to find feminist meaning from Son So-hee’s novels because they depict so-called passive woman, and to actively examine the representation of female voices in her literature. Based on Gayatri Spivak’s critical theory, which focuses on the impossibility of Subaltern’s voice and Gérard Genette’s concept of Focalization in order to understand the structure of the narrative text based on the relationship between the focalized character and the narrator, this research pays attention to two short stories written by Son, So-hee. First, When Iris Blooms uses the death of a female character in a confession epic in which the main character, I, was transformed from a boy to a patriarchal man. The hidden female voice remains as a blank of text that is impossible to be represented in the discourse of focalized male character and male narrator, and at the same time, implies the divisibility of the male-subject while being a practical condition that constitutes the subject. On the other hand, Confrontation II has a focalized female character being as the eyewitness to the incident, Marie, who catches the woman’s desire of an escape not to be captured by the violent eye of men. The traces of a woman’s voice that emerges through the blank finally open up the possibility of testimony. 이 글은 손소희 소설이 ‘수동적인’ 여성상을 그리고 있어 페미니즘적 의미를 발견하기 어렵다는 종래의 비판에 반박하며, 손소희 문학에서 여성의 목소리가 재현되는 양상을 적극적으로 고찰해 보려는 목적에서 출발하였다. 이에 서발턴의 재현 불가능한 목소리에 주목하는 가야트리 스피박의 비평 이론을 토대로, 초점 인물과 서술자의 관계로부터 서사 텍스트의 담론 구조를 파악하기 위해 제라르 즈네뜨의 초점화 이론을 분석틀로 삼아 두 편의 단편소설을 탐색하였다. 먼저 「창포 필 무렵」의 경우 초점 인물 ‘나’가 소년에서 가부장-남성 주체로 변모해 가는 고백의 서사에서 여성 인물의 죽음을 동원하고 있었다. 은폐된 여성의 목소리는 남성 초점 인물과 서술자의 담론에서 재현 불가능한 ‘공백’으로 남음과 동시에, 주체를 구성하는 실정적 조건으로 작용하며 남성-주체의 분열성을 암시한다. 한편 「靜·動<對決Ⅱ>」은 ‘마리’라는 여성을 초점 인물이자 사건의 ‘목격자’로 등장시켜 남성의 폭력적인 시선에 포획되지 않는 여성 욕망의 잔여를 포착하도록 한다. 이러한 목격자에의해 서사적 공백을 뚫고 솟아오르는 여성 목소리의 흔적은 증언의 (불)가능성을 열어젖히게 된다.

신체화장애 환자의 스트레스 대처방식 및 심리적 특성

이주연,이무석,윤진상,양종철,문지웅,정해원,은성종 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.6

Objectives : The purpose of this study was to investigate the stress coping strategies and psychological charactehstics, such as combined psychopathology and tendency of symptom interpretation, in patients with somatization disorder. Methods : Thirty patients meeting the criteria of DSM-IV somatization disorder were compared with thirty controls. We evaluated the subjects using Symptom Checklist-90-Revised (SCL-90-R), Somato-Sensory Amplification Scale (SSAS), Symptom Interpretation Questionnaire (SIQ), and The Ways of Stress Coping Questionnaire (SCQ). Independent t-test and Pearson correlation analysis were used. Results : From the results of SCL-90-R subscales, the scores of somatization, obsession-compulsion, depression, anxiety, and psychoticism were significantly higher in patients with somatization disorder than nonnal controls. Somatization disorder patients had greater amplification of physical sensation in SSAS and significantly higher score in physical interpretation of SIQ compared with psychological or environmental interpretation, In the SCQ of somatization disorder patients, we observed generallylower levels of total coping scores than the control group and significant positive correlation between passive coping style of SCQ and psychological interpretation of SIQ. Conclusion : These results show that patients with somatization disorder have various psychopathology, greater amplification of physical sensation, physical interpretation tendency of symptoms, and insufficient copying strategy.

농촌 주부의 의복 활용과 구매 행동에 대한 탐색적 연구

이지연,박재옥,조현숙 漢陽大學校 韓國生活科學硏究所 2000 韓國 生活 科學 硏究 Vol.- No.18

The purpose of this study was to investgate the current trends of clothing practical use and buying behavior of rural women. Subjects of this study were 200 women residing in 3 rural areas in Korea. The data were obtained through questionnaires which handled by the trained Interviewers, from June in 1997 to July in 1997. Data were analyzed by frequency, percentage, cross∼tabulation, Chi square test. Based on the results of this study findings were as follows. 1.More than half women in rural areas ware jacket with pants as formal wear and T-shirts with pants as casual wear. 2.Planned purchase is more often the case of formal wear than that of casual. What motivates to purchase formal wear turns out to be 'I don't have one' with constant to 'look comfortable' which is predominant in the case of casual wear The evaluative criteria of clothing was " color and design" in the case of formal wear wheres "comfort and fitting" in the case of casual wear. The buying place is usually a market having near location, big and small. As so women in rural areas prefer relatively chief clothing and payed by cash. They tend to be satisfied with what they purchased. In a few case of dissatisfaction, they exchange or return the formal wear, but they repair the casual wear. 3.In the case of formal wear, the price or the ratio of discount is the most important store selection criteria and the store display is the most important information source. Also, they usually purchase clothing for ceremony in the case of formal wear. In the case of formal wear, age makes difference on place and time however buying place is dependent upon income. Education level, on the other hand, makes significant difference in buying time. In the case of casual wear, age, education, income makes difference on buying price, evaluative criteria.