Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

박목월의 시 <나그네>의 예술가곡화 연구

이지연 ( Lee Ji-yeon ),김용범 ( Kim Yong-bum ) 한민족문화학회 2016 한민족문화연구 Vol.56 No.-

This study aims to examine the capability of Mok-wol Park`s poetry, Naguenae, as an art song. The art song, originally developed in Germany in the eighteenth-century, is a genre of art that unites poetry and music; the composers put a melody on poems so that singers could sing the songs. As an outcome of cultural convergence, the two independent forms of art, literature and music, came to converge into a completely single genre, heralding a birth of a new musical style. Korean poetry began to mature in the 1930s and Mok-wol Park, the most representative poet throughout the history of Korean literature, shows lyrical and rhythmical features on his early poems. This musicality was valuable to be used as text for art songs by many contemporary composers such as Yeon-jun Kim, Sung-tae Kim, Sun-ae Kim, Soo-in Lee, and I-sang Yoon. In the process of categorizing their art songs, we discovered that there are numerous songs using particularly Naguenae as a text. This study mainly explores and analyzes reasons from musical and glossematic perspectives, as well as from viewpoint of the theory of cultural convergence. Mok-wol`s Naguenae written based on a calculation of its regular rhythmic patterns has the optimum condition as a text for the musical setting in which a tonal center, rhythm, and phrasing can be combined without any disturbances. Moreover, his use of liquid sound aids a singer`s vocalization. Therefore, in view of the fact that a song is to be sung, which is a necessary and sufficient condition for an art song, together with a text and musical setting, Naguenae qualifies all conditions. The fact that one particular poem written by one poet was used as a text for an art song by fourteen different composers is unusual case in Korean history of art songs. In addition, Mok-wal Park`s Naguenae is not only literary valuable, but means a new step of cultural convergence.

가평 전주이씨묘 출토복식 고찰

최연우,박윤미,김윤경,김지희,박양희,이선우,차서연,편나영,황혜남,Choi, Yeon Woo,Park, Yoon Mee,Kim, Yoon Gyung,Kim, Ji Hee,Park, Yang Hee,Lee, Seon U,Cha, Seo Yeon,Pyeon, Na Young,Hwang, Hye Nam 한국의류학회 2017 한국의류학회지 Vol.41 No.5

In 1995, the costumes were restored in an unknown woman's tomb at the Jeonju Lee's family cemetery in Gapyeong, Gyeonggi-Do, and there are currently nine items remaining. In this study, we first introduced these 9 relics to academia and analyzed the morphological characteristics of the costumes. We also estimated the time and person of burial compared with costumes unearthed from other burials. Jeogories (short jacket) are all four items. One of these items was unusual in shape, and the upper part of the seop (gusset) was wrinkled and shaped. This type of Jeogori appears only till the 1520s in other tombs. There are also two skirts, one of which is characterized by a superimposed pattern. The top and bottom / middle part of the skirt were rolled up, and the skirt, which was double-rolled up in this way, was first discovered. An analysis of the shape of Jeogori and skirt indicated that the person buried in the Jeonju Lee's family tomb was believed to have survived until the early 16th century. Based on these estimated periods, as a result of looking at the genealogy of Jeonju Lee's family, it was concluded that the tomb was probably a woman named Lee Geum Myeong (李金命) born in the mid-15C and died between the late 15C and the early 16C.

한ㆍ미 문화특성 차이에 따른 호텔선택속성 비교분석

이화인,김지연 문화관광연구학회 2000 문화관광연구 Vol.2 No.2

This study investigated how hotel customers' choice attibutes differ in terms of their importance, depending upon the cultural background of the customers. Specifically, this research examined the differences in cultural characteristics and analyzed their influence on choice behavior of Korean and American customers. The results of the empirical analysis indicated that collectivism and future orientation are two major cultural factors that classify Korean and American cultural characteristics.

한국 전시·컨벤션 센터의 운영 실태 및 입지에 관한 연구

이성호,방연종,김지숙 釜山大學校 都市問題硏究所 2003 都市硏究報 Vol.15 No.-

The Exhibition & Convention centers in Korea have many problems because of the constructions with overcompetition by local government. The purpose of this study therefore, is to explore the actual condition and locational suitability of the five existing exhibition & convention centers in Korea, and to suggest criteria on the location when same facility is constructed hereafter. From now on, locational criterion should be applied more cautiously and in detail, so the locational suitability must be heighten. There are some limits in this study. Most of all, it must be overcome the subjectivity of locational criterion, so various studies must be accomplished hereafter.

다중지능 프로그램이 유아영재의 엉어, 수, 사회성에 미치는 효과

이신동,오연경,박지희 순천향대학교 인문과학연구소 2008 순천향 인문과학논총 Vol.22 No.-

The purpose of this study is to investigate the effect of a multiple intelligence program on the language, mathematics, and social behaviors of the gifted. The subjects were 20 gifted children who attended the Education Center in Seoul. They were 3-5 years old and their intelligence quotients were ranged between 125 and 140 measured by the K-WPPSI. Three tests were used in the study. First, the language ability test constructed by Young-Ae Jang (1981) was used for 4-6 years old children. Second, the test for the concept of numbers was designed to find out the achievement level of the number concept developed by Young-Hee Yeo (1995). Third, for the social behavior test questions that correspond to children aged between 3 and 5 were used. The results of this study were as followings: First, the multiple intelligence program positively affected the development of linguistic ability of the gifted children, with a significant difference in the development of linguistic expression. Second, the program based on multiple intelligence positively influenced the development of number concept of the gifted children. And third, multiple intelligence program has positively influenced the development of socialization of the gifted children, especiallv in terms of communication abilities.

갑상샘암 수술환자의 심리 및 신체적 불편감에 미치는 정보교육 프로그램 효과

이양희,정영,박연주,최길순,정지연 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

본 연구는 정보교육 프로그램이 갑상샘암 수술 환자의 심리 및 신체적 불편감 감소에 미치는 효과를 검정하기 위해 실시된 비동등성 대조군 전,후 시차 설계의 유사 실험연구이다. 자료수집 기간은 2006년 6월 17일 부터 9월 19일 까지 3개월간 이었으며, 연구 대상자는 일개 종합병원에 갑상샘암 수술을 받기위해 입원한 환자 가운데 6월 17일 부터 8월 3일 까지 입원환자 29명을 대조군으로 하였고, 8월 5일 부터 9월 19일 까지 입원환자 30명을 실험군으로 하였다. 연구도구는 심리적 불편감을 측정하기 위해 Derogatis 등(14)이 개발한 ‘자기보고식 다차원 증상목록(Multidimensional Symptom Inven- tory)검사를 사용하였고, 신체적 불편감 측정은 McCorkle & Young(16)이 개발한 도구를 사용하였다. 본 연구의 결과는 다음과 같다; 1) 정보교육 프로그램을 받은 실험군은 정보교육 프로그램을 받지 않은 대조군에 비해 심리적 불편감 정도의 차이는 통계적으로 유의한 차이를 보이지 않았다(p= .135). 다만 ‘근심 걱정’요인에서 유의한 불편감 감소효과를 보였다(p= 0.49) . 2) 정보교육 프로그램을 받은 실험군과 정보교육 프로그램을 받지 않은 대조군은 두군 모두 신체적 불편감 정도가 사후 유의한 수준으로 증가함을 보였다(p= 0.90). 결론적으로 본 연구를 위해 만들어진 정보교육 프로그램은 갑상샘암 수술 환자의 신체적 불편감 감소에는 효과적이지 못하였으나 심리적 불편감의 근심걱정 요인을 감소시키는데는 효과가 있음을 알게 되었다. Objectives: This study was designed as a non-equivalent control group pre and post-test quasi-experimental study to examine the effects of education program using information on the psychological and physical discomfort of patients undergoing operation for cancerous thyroid glands. Methods: The study covered for three months from June 17, 2006 to September 19, 2006. The subjects of this study were selected from those patients who were admitted in one of the general hospitals in order to receive the operation for cancerous thyroid glands. They include 29 control group patients who were hospitalized during the period of Jun 17th to August 3rd 2006, and 31 experimental group patients who were hospitalized during the period of August 5th to September 19th, 2006, respectively. The instruments utilized for conducting this study includes the Multidimensional Self-Report Symptom Inventory test originally developed by Derogatis & et al. (1983) for measuring psychological discomfort. And for measuring physical discomfort, the tools developed by McCorkle & Young (1978) was utilized. Results: 1) Hypothesis 1 “The level of psychological discomfort of the experimental group who completed the information education program would be decreased comparing with that of the control group who didn't complete the program” was partly supported (Worry & Anxiety factor: p= .049). 2) Hypothesis 2 “The level of physical discomfort of the experimental group who completed the information education program would be reduced comparing with that of the control group who didn't complete the program” was not supported (p= -.90). Conclusion: The information program provided for this study was proved to be not effective for decrease physical discomfort of the patients undergoing operations for cancerous thyroid glands but proved to be effective for decrease the anxiety factor of psychological discomfort.

개선된 중성염 전해공정을 이용한 모의 방사성금속폐기물의 제염

이지훈,육완이,양호연,하종현 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.2

원자력발전소에서 주로 발생되는 금속폐기물인 탄소강을 중성염전해질인 1.7M의 황산나트륨(Na₂SO₄)과 질산나트륨(NaNO₃)을 이용하여 기존전해제염과 개선된 전해제염공정의 비교실험을 수행하였다. 양극은 인코넬, 음극은 티타늄으로 하여 상온에서 1시간동안 반응시켜 금속폐기물 모재의 weight loss, 두께변화, 전해질 내 침전물농도, SEM을 이용하여 제염전후의 금속폐기물 표면의 형상을 분석하였다. 실험결과 개선된 전해제염 적용시 전해질 종류별 전류밀도 변화에 따른 실험에서는 전류밀도가 0.1∼0.6A/cm²으로 증가함에 따라 1.7M의 황산나트륨 적용시 금속폐기물 모재의 두께변화는 0.48±0.005∼67.7±0.02um, 1.7M의 질산나트륨 적용시에는 0.06±0.005∼17.7±0.05로 나타나 같은 전류밀도에서 황산나트륨 적용시 금속폐기물 모재의 표면 제염효율이 더욱 높은 양상을 보였다. 또한 전류밀도 0.3 A/cm² 및 1.7M의 황산나트륨의 조건에서 개선된 전해제염 적용 시 9.8±0.01um의 금속폐기물 두께변화를 보여 기존전해제염 적용시인 3.7±0.03um의 금속폐기물 두께변화보다 2배 이상의 표면 제염효과를 보였다. Conventional and modified electrolytic decontamination experiment were performed in the 1.7 M solution of sodium sulfate and sodium nitrate for decontamination of carbon steel as the simulated metal wastes which have been produced in large amounts from nuclear power plants. Anode and cathode were used as inconel and titanium respectively. The reaction time and temperature were 1 hr and 25℃. The analyses were performed of the characteristics such as weight loss and thickness change of metal waste, suspended solid in electrolyte and SEM observation. In modified electrolyte decontamination system with increased current density ranged from 0.1 to 0.6 A/cm², the metal waste showed thickness changes of 0.48±0.005 to 67.7±0.02um in 1.7 M sodium sulfate and those of 0.06±0.005 to 17.7±0.05um in sodium nitrate. Metal waste in modified electrolyte decontamination system showed the thickness change of 9.8±0.01um while it reacted up to 3.7±0.03um in conventional system with 0.3 A/cm² of current density and 1.7 M sodium sulfate. Decontamination efficiencies of modified electrolytic process are much higher than that of conventional electrolytic process when both are applied to metal waste.

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

신경모세포종의 화학 및 방사선요법이 치아우식활성도에 미치는 영향

이지현,박기태,성기웅,김지연 大韓小兒齒科學會 2010 대한소아치과학회지 Vol.37 No.3

소아암 환자의 치료 방법으로 주로 사용되는 항암화학요법이나 방사선요법이 구강 건강에 미치는 영향은 매우 크다. 구강 점막염, 타액 분비율의 변화, 구강내 감염을 야기할 수 있고, 부분무치증, 왜소치, 치근 발육이상, 저석회화증이 나타날 수 있다. 본 연구는 신경모세포종으로 치료 받은 환아들에 있어 항암치료가 치아우식증 발생 위험도에 어떠한 영향을 미치는지 알아보기 위해 실시되었다. 삼성서울병원 소아청소년과에 신경모세포종으로 내원하여 화학 및 방사선치료요법을 받은 환아들을 대상으로 Dentocult.. SM, Dentobuff.. Strip을 이용하여 항암치료 후의 타액 분비율, 타액 완충능, 타액 내의 Streptococcus mutans 수를 알아보고 정상 어린이들의 결과와 비교하였으며, 신경모세포종 환아에서 처음 치료를 시작한 나이, 치료 종결 후 경과 시간에 따라 치아우식활성도가 어떻게 달라지는지에 대해서도 알아보았다. 그 결과 타액 분비율은 환아가 대조군에 비해 유의성 있게 낮은 결과를 보인 반면(p<0.05), 타액 완충능과 Streptococcus mutans 수는 환아와 대조군 간에 유의성 있는 차이를 보이지 않았다. 환아에서 치료를 시작한 나이나 치료 종결 후 경과 시간에 따른 치아우식활성도에서도 통계적 유의차는 없었다. Chemotherapy or radiotherapy used for the treatment of pediatric cancer may have many adverse effects on the oral cavity. Oral mucositis, reduced salivary flow, oral infection, hypodontia, microdontia, arrested root development, and enamel hypoplasia are common oral complications. The aim of this study is to evaluate the effects of cancer therapy on dental caries activities. The children who had been treated for neuroblastoma in the department of pediatrics, Samsung Medical Center, were included and healthy children served as controls. The salivary flow rate, salivary buffering capacity, and Streptococcus mutans counts of both groups were evaluated using Dentocult SM and Dentobuff Strip. The dental caries activity related to the age at the start of treatment and the time elapsed since treatment completion were also evaluated. As a result, neuroblastoma patients had significantly lower salivary flow rate than the controls, while there were no significant differences between two groups as for salivary buffering capacity and Streptococcus mutans counts. The dental caries activities related to the age at the start of treatment and the time elapsed since treatment completion were not significantly different.