셀레콕시브 및 그 합성유도체들의 항암활성 스크리닝

박정란,강진형,구효정,노지영,류형철,박상욱,고동현,조일환,이주영,황다니엘,김인경 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Selective COX (cyclooxygenase)-2 inhibitors including celecoxib have been shown to induce apoptosis and cell cycle changes in various tumor cells. New inhibitors are recently being developed as chemomodulating agents. We evaluated celecoxib and screened 150 synthetic compounds for anti-proliferative activities in vitro. Effects of celecoxib on COX activity, cell growth, cell cycle distribution, and apoptosis induction were determined in A549 COX-2 overexpressing human non-small cell lung cancer (NSCLC) cells. The COX inhibition of celecoxib increased with concentration up to 82% at 1μM after 24 hr exposure. Forty μM and 50μM of celecoxib induce G_1 arrest, and TUNEL-positive apoptotic cells, respectively. Among 150 compounds, several compounds were selected for having greater COX-2 inhibitory activity and higher selectivity than celecoxib with growth inhibitory activity. Celecoxib showed concentration-dependent COX inhibitory activity, and ability to induce cell cycle arrest and apoptosis in human NSCLC cells in vitro. Among synthetic analogues screened, several compounds showed promising in vitro activity as COX-2 inhibitory anticancer agents, which warrant further evaluation in vitro and in vivo.

위의 유암종과 선종의 병발 1례

장재식,강혁주,이중현,서영범,윤병구,김용섭,이구,서정일,양창헌,이창우,김정란,윤환중 東國大學校醫學硏究所 2000 東國醫學 Vol.7 No.-

유암종은 전신에 퍼져 잇는 장크롬친화성 세포에서 기원하는데 위의 유암종은 비교적 드문 질환으로 모든 유암종의 1.9~2.2%에 해당하며 위에 발생하는 종양의 1% 미만에서 보고되고 있다. 유암종은 일반적으로 증상이 비특이적이고 면역조직화학검사에 의해서 진단되기 때문에 과거에는 드물게 발견되었으나 최근 내시경 기기 및 술기의 발전과 검사 빈도의 증가 및 면역조직화학검사의 발달로 진단율이 증가 추세에 있으며, 드물지만 유암종에서 선암과 공존하는 예들이 보고되고 있다. 저자들은 심와부 동통을 주소로 내원한 70세 남자 환자의 상부위장관내시경 검사에서 위체상부의 대만곡에서 0.4×0.4 cm 크기의 중간 함몰을 가진 Yamada 1형의 용종과 함께 전정부의 전벽에서 0.8×0.5 cm 크기의 융기된 점막 소견을 보여 조직학적으로 각각 위유암종 및 위선종임을 확진한 후 내시경적 용종 절제술로 치료한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Carcinoid tumors are arisen from enterochromaffin cells, which are scattered throughout the body. These tumors are composed of variable numbers of cells that contain endocrine granules in their cytoplasm, which can be identified by immunohistochemistry and electron microscopy. Gastric carcinoid tumors are relatively rare tumor, the reported incidence being 1.9~2.2% of all carcinoid tumors and less than 1% of all gastric tumors. Sometimes, composite carcinoid tumors and adenocarcinoma is reported. We experienced a case of the coincidence of carcinoid tumor and adenoma at the other site of the stomach in a 70 year old male patient. Gastrofiberscope showed 0.4×0.4 cm sized Yamada type I polyp with central depression on the greater curvature of the proximal body and 0.8×0.5 cm sized focal slightly elevated mucosa on the anterior wall of the antrum. The endoscopic biopsy specimens revealed carcinoid tumor and tubular adenoma with moderate atypism, respectively. The carcinoid tumor and adenoma were removed by endoscopic polypectomy. According to the follow-up gastrofiberoscopy after 7 month later, both lesions were nearly normal mucosa except whitish atrophic change after polypectomy.

Erratum to: Intracellular Substrates of a Heme-Containing Ascorbate Oxidase in Pleurotus ostreatus

Seung-Rock Lee,Woo-Jeong Joo,Yong-Un Baek,Youn-Kyong Lee,Seong-Woon Yu,Yeon-Ran Kim,Kee-Oh Chay,Seung-Hyun Cho,Sa-Ouk Kang 한국미생물학회 2009 The journal of microbiology Vol.47 No.6

In the online version of the article the name of the second corresponding author is misspelled. The correct spelling is Sa-Ouk Kang. The online version of the original article can be found under doi: 10.1007/s12275-008-0307-8

한약에 의해 유발된 급성 간질성 신염 2례

김덕윤,박동건,김응석,강영모,양창헌,이정호,이동철,이영현,김정란 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

간질성 신염은 신장의 간질을 선택적으로 침범하는 염증성 질환으로 여러가지 원인에 의해 유발되며, 이 중 약물에 의한 경우는 각종 항생제, 비스테로이드성 진통제, 항경련제, 이뇨제, 면역억제제등에 의한 증례들이 보고되고 있다. 현재 각종 질환-특히 만성질환-의 치료에서 한약이 차지하는 비중이 적지 않으나, 그 각각의 성분들이 유발할 수 있는 부작용들에 대한 연구는 거의 없는 실정이다. 저자들은 관절염 치료를 위해 중국산 한약을 복용한 후 복통, 피로감 및 육안적 혈뇨를 주소로 내원한 두 환자에서, 단백뇨와 신기능 장애를 보여 시행한 신조직 생검상 급성 간질성 신염에 합당한 병리학적 소견을 보이고, 한약 복용 중지 후 급속한 회복을 보여, 한약에 의해 유발된 것으로 사료되는 급성 간질성 신염 2예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Acute interstitial nephritis is a disease characterized by renal interstitial inflammatory cell infiltration and acute renal functional deterioration. This is caused mainly by antibiotics, NSAID and diuretics such as thiazide, but cases induced by herb medication are rare. We experienced two cases of acute interstitial nephritis after treatment with herb medication. One 71-year-old female patient and the other 60-year-old female were admitted to the hospital because of general weakness and gross hematuria. Microscopic hematuria, pyuria, and proteinuria were presented. After definitive diagnosis with a renal biopsy, we noted rapid recovery of renal function by drug withdrawal and steroid therapy. We report these cases with a review of the referenced literatures. Key Words : Herb medication, Acute Interstitial nephritis.

연쇄알구균 감염에 의한 급성 인후염 후 반응성 관절염 1예

박은하,도연실,양정채,강미라,서현주,정숙인,손준성,기현균,오원섭,백경란,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

연쇄알구균에 의한 급성 인후염 후 발생한 반응성 관절염은 급성 류마티스열의 개정된 진단기준을 충족하지 못하면서, 지속적 혹은 재발성의 비이동성 관절염과 발열, 홍반성 결절, 일시적 간기능 이상 등을 보이면서 심장염과 무도증을 드물게 동반하는 질환이다. 따라서, 발열을 동반한 급성 관절염 환자에서 감별해야 할 질환 중의 하나이며, 심근염 예방을 위한 페니실린 요법의 적응증 및 기간에 대해서는 아직 이론이 많은 상황으로, 보다 광범위한 연구가 필요하다. 저자들은 연쇄알구균에 의한 급성 인후염 1주 후에 발생한 하지의 다발성 비이동성 관절염과 함께 결절홍반 및 일시적 간기능 이상을 보인 환자에서 PSRA로 진단하고 비스테로이드성 항염증제 및 스테로이드로 성공적인 치료를 하였기에 이를 문헌 고찰과 함께 보고하는 바이다. Poststreptococcal reactive arthritis (PSRA) is associated with recent streptococcal infections. However, PSRA is distinct from acute rheumatic fever by its clinical manifestations: non-migrating arthritis, erythema nodosum or erythema multiforme, and transient elevation of serum transaminases. We experienced a 33-year-old man who presented with fever, arthritis of both knees and ankles, and erythema nodosums on extensor surfaces of lower legs which developed 6 days after the onset of pharyngitis symptoms. Blood and urine cultures were negative. Throat culture was negative for group A β-hemolytic streptococcus. The ASO titers increased up to 2080 IU/mL in sequential monitoring. The result of bone scan was compatible to arthritis of both knees and ankles. There were no signs or symptom of carditis. He showed clinical improvement with anti-inflammatory drugs (naproxen 1,000 mg/day and prednisolone 7.5 mg/d). PSRA should be included in the differential diagnosis of patients presenting with arthritis combined with fever.

A Study of Physiological Activities for Cosmeceutical Ingredient from Fermented Aroniamelanocarpa Extract

Jeong Ran Kang,Dong-Soon Oh,Xiao Xiao Huang,Jong-Hwa Kim,Kap-Hoon Han 중소기업융합학회 2020 융합정보논문지 Vol.10 No.1

아로니아는 특유의 신맛과 떫은 맛 때문에 생과보다는 가공용으로 개발할 필요성이 대두되고 있다. 유산균 을 이용한 아로니아분말 발효추출물에 대한 항산화 활성 및 세포독성을 검정한 결과 총 폴리페놀 함량의 경우 발효전 32.15 μg/mg, 발효 후 43.08 μg/mg으로 증가하였고, 플라보노이드 함량은 발효전 0.47 μg/mg, 0.44 μg/mg 으로 조금 높거나 유사하게 나타내었다. DPPH radical 억제 활성은 발효전 77.5% 에서 89.1%로 발효전 보다 조금더 높은 활성을 나타냈으며, Nitric oxide (NO) 측정은 농도 의존적인 억제효능이 나타났다. 이상의 결과로부터 아로니아분말을 발효할 경우 일부 항산화 활성을 기반으로한 활용가능성과 향후 식물성 천연 추출물 과 발효 화장품 소재로서의 이용가능성을 확인할 수 있었다. Due to the unique sourness and sweet taste of Aronia, it is necessary to develop it for processing rather than raw. The antioxidant activity and cytotoxin about the aronia powder fermentation extract using lactobacillus are verified. In the case of total polyphenol content, the content of non-fermented extract was 32.15 μg/mg fermentation extract, 43.08 μg/mg after fermentation, and the flavonoid content was 0.47 μg/mg in non-fermented extract and 0.44 μg/mg in fermented extract, which was similar to that of non-fermented extract. In the DPPH radical inhibition assay, 77.5% of the non-fermented extract and 89.1% of the fermented extract showed better activity than the non-fermented extract. Nitric oxide (NO) measurement showed a concentration-dependent inhibitory effect. From the above results, it was confirmed that the fermentation of Aronia powder could be utilized based on some antioxidant activities and the possibility of using it as a vegetable extract and fermented cosmetic material in the future.

Tyrosinase Inhibitory Activity of Rosmarinic Acid on Human Skin Melanoma Cells Treated with Hydrogen Peroxide

Jeong-Ran Kang,Sun-Mi Yoo 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.2

The purpose of this study was to evaluate the tyrosinase inhibitory activity of rosmarinic acid on the cultured human skin melanoma cells damaged by reactive oxygen species (ROS). The cytotoxicity effect was analyzed by MTT assay for cell viability, tyrosinase inhibitory activity after treating the cells with or without hydrogen peroxide (H₂O₂) for 1-10 hours. Light microscopic study was also carried out for morphological changes of the cells and tyrosinase activities. H₂O₂ decreased the cell viability in the cultured human melanoma cells in a dose- and time-dependent manner after cells had been treated with 20-80 ?M of H₂O₂ for 7 hours. IC?? value of H₂O₂ by the MTT assay was 80 ?M. Rosmarinic acid increases cell viability, inhibitory activity of tyrosinase. In light microscopic study, the number of human skin melanoma cells treated with H₂O₂ was decreased compared with control, and H2O2 activated tyrosinase activity, which was observed through darkened cell color (mid-activity, +++). However, colors of cells treated with Rosmarinic acid changed from white to brown in cell staining due to tyrosinase activity (moderate-activity, ++). The treatment of the cells with H₂O₂ shows highly toxic effects on cultured human skin melanoma cells. Rosmarinic acid increases cell viability and tyrosinase inhibitory activity in the cells treated with H₂O₂. It is considered that Rosmarinic acid shows tyrosinase inhibitory effect on ROS such as H₂O₂.

Regulatory Mechanisms Involved in Proteolysis of Rice Leaves during Senescence

KANG, SEONG MO,KANG, NAM JUN,LEE, NAM JIN,KANG, JUN SOON,KIM, YOUNG RAN,JEONG, YEON OK,PARK, JOONG CHOON,KIM, ZHOO HYEON,UM, SUNG KYUN,CHO, JEOUNG LAI 경상대학교 유전공학연구소 1990 遺傳工學硏究所報 Vol.9 No.-

NaCl and KCl enhanced the degradation of chlorophylls and proteins in detached rice (Oryze sativa) leaves in a concentration-dependent manner. Ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco) accounted for 73 to 80% of the protein lost by day 4 in the light. NaCl at 50mM increased proteolysis by 21% over the control in 4 days, but the addition of cycloheximide reduced the increase to about one-half. Cycloheximide alone had no effect on proteolytic activity during this period. Leaf segments taken from 10-day-old seedlings contained the highest proteolytic activity. Both NaCl and KCl increased the activity of Rubisco-degrading endoproteinases, but decreased the activity of hemoglobin-and Rubisco-degeading exoproteinases. Efflux of amono acids from senescing leaf segments into the incubation media increased 7- and 12- fold in the presence of KCl and NaCl, respectively. The effect of gibberellic acid(GA₃) and abscisic acid(ABA) on KCl-enhanced proteolysis was also studied. When treated singly, GA₃affected leaf proteolysis little, while ABA increased proteolysis, the rate of amino acid efflux, and Rubisco-degrading endo-proteinase activity. An additive increase in all three parameters mentioned above was observed when leaves were treated with ABA and KCl. No such an additive effect was found when GA₃was treated with KCl. Both GA₃and ABA helped to alleviate the KCl-suppressed activity of Rubisco-degrading exproteinases. The additive increase in proteolysis of rice leaves in the presence of both ABA and KCl could thus be ascribed to a further increase in the efflux of protein hydrolyzates and Rubisco-degrading endoproteinase activity. It is concluded that, in addition to the induction of new proteinase synthesis, the increased efflux of protein hydrolyzates may play a significant role in increasing proteolysis of salt-treated leaves.

Comparison of Biological Activities on Extracts and Fractions in Compositae Plants

Jeong-Ran Kang,Eun-Mi Yu,Kap-Hoon Han 중소기업융합학회 2019 융합정보논문지 Vol.9 No.9

본 연구는 우리나라 전역에 자생하는 국화류3종 학슬, 희렴, 한련초를 대상으로 MeOH 80% 추출물 및 헥산, 클로 로포름, 에틸아세테이트, 부탄올 및 물분획을 이용한 항균활성, 항산화 및 tyrosinase 저해활성효과에 대한 연구를 통해 기능성 화장품소재로서의 이용가능성을 확인하고자 하였다. 연구결과 항균활성의 경우 MeOH 80% 추출물에서는 한련초 와 용매분획 중에서는 학슬의 헥산분획, 희렴의 클로로포름분획, 에틸아세테이트 분획, 한련초의 클로로포름분획을 중심 으로 항균력을 나타내었다. 항산화 활성의 경우 3종 모두 용매분획 중 에틸아세테이트, 부탄올 분획에서 90%이상의 효과 를 나타내었다. Tyrosinase 저해활성의 경우 희렴과 학슬의 에틸아세테이트 분획물에서 우수한 활성을 나타내었다. 이상 의 국화류3종 MeOH 80%추출물 및 분획물에서 항산화 및 미백작용이 우수하므로 기능성화장품소재 개발 활용이 가능할 것으로 생각된다. This study was conducted to investigate the anti-oxidative, anti-oxidative and tyrosinase inhibitory effects of MeOH 80% extract and hexane, chloroform, ethyl acetate, butanol and aqueous fraction on three kinds of compositae plants in Korea. In the antimicrobial effect, the extract and chloroform fraction of Eclipta prostrata and hexane fraction of Carpesium abrotanoides L. and chloroform fraction of Siegesbeckia glabrescens exhibited significant inhibition. The antioxidant activity of ethyl acetate and butanol fractions was more than 90% in all three plants. In case of tyrosinase activity, showed a potent inhibition ethlyacetate fraction of Siegesbeckia glabrescens and Carpesium abrotanoides L, which were higher than control group. In MeOH 80% extracts, there was not found to have antimicrobial, anti-oxidant and tyrosinase inhibitory activity, however there was ethylacetate fraction of Siegesbeckia glabrescens to show effectss commonly in the three assay system.

[P5-21] Expression of Alpha-galactosidase gene from Leuconostoc mesenteroides SY1 in Lactobacillus brevis 2.14

Kang wook Lee,Gun-Hee Kwon,Gyoung Min Kim,Ae Ran Lee,Woo Ju Jeong,Jae-Yong Park,Seon-Ju Jeong,Ji-Yeon Chun,Jeong Hwan Kim 한국식품영양과학회 2007 한국식품영양과학회 학술대회발표집 Vol.2007 No.10