DJ-1 deficiency impairs synaptic vesicle endocytosis and reavailability at nerve terminals

Kyung, Jae Won,Kim, Jin-Mo,Lee, Wongyoung,Ha, Tae-Young,Cha, Seon-Heui,Chung, Kyung-Hwun,Choi, Dong-Joo,Jou, Ilo,Song, Woo Keun,Joe, Eun-Hye,Kim, Sung Hyun,Park, Sang Myun National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.7

<P>Mutations in DJ-1 (PARK7) are a known cause of early-onset autosomal recessive Parkinson's disease (PD). Accumulating evidence indicates that abnormalities of synaptic vesicle trafficking underlie the pathophysiological mechanism of PD. In the present study, we explored whether DJ-1 is involved in CNS synaptic function. DJ-1 deficiency impaired synaptic vesicle endocytosis and reavailability without inducing structural alterations in synapses. Familial mutants of DJ-1 (M26I, E64D, and L166P) were unable to rescue defective endocytosis of synaptic vesicles, whereas WT DJ-1 expression completely restored endocytic function in DJ-1 KO neurons. The defective synaptic endocytosis shown in DJ-1 KO neurons may be attributable to alterations in membrane cholesterol level. Thus, DJ-1 appears essential for synaptic vesicle endocytosis and reavailability, and impairment of this function by familial mutants of DJ-1 may be related to the pathogenesis of PD.</P>

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

‘임상간호연구’ 학술지 게재논문(1995-2008년)의 연구동향 분석

최명애,정재심,임경춘,김주현,김금순,권정순,김성재,김경희,곽찬영,박광옥,이경은,김을순,이경숙 병원간호사회 2010 임상간호연구 Vol.16 No.2

Purpose: The purpose of this study was to identify the research trend of 341 studies published in Journal of Korean Clinical Nursing Research from 1995 to 2008. Methods: This study was conducted from May to November, 2009. The tool for analysis developed through literature reviews and discussions of researchers consisted of 7 categories such as characteristics of researchers, research designs, subjects, data collection methods, ethical consideration, nursing interventions, and dependent variables. Data were analyzed by frequency and percentage. Results: The mean number of author per study was 4.7, and clinical nurses were the most frequent as the first author (79.7%) and as a corresponding author (67.1%). Quantitative study was 97.6% and nonexperimental study was 51.7%. The most frequently used research designs were quasi-experimental study (73.9%) among experimental studies and survey study (79.1%) among nonexperimental studies. Patients were the most frequent subjects for studies (55.8%). 46.1% of studies gathered data with questionnaire, 57.7% of studies had consent from patients, and 44.3% of studies used nursing skills for nursing interventions, and 60.0% of studies used clinical end points for dependent variables. Conclusion: The research trend of clinical nursing studies shows that clinical nurses mostly perform quasi-experimental researches to solve patients' problem and frequently use nursing skills for nursing intervention and explore the effect of nursing interventions on clinical end points.

봉독약침이 류마티스 관절염 환자의 기능회복 및 삶의 질에 미치는 영향

이상훈,이현종,박상민,김수영,박재경,홍승재,양형인,이재동,최도영,김건식,이두익,이윤호 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Objective: To evaluate the effects of bee venom acupuncture(BVA) on the rehabilitation and quality of life in rheumatoid arthritis(RA) patients Methods: Patients with RA were treated with the BVA therapy twice a week for 3 months. Tender joint counts, swollen joint counts, morning stiffness, Erythrocyte Sedimentation Rate(ESR), C-reactive protein(CRP), patient global assessment, physician global assessment, Korean health assessment questionnaire(KHAQ) were estimated and analyzed before and after BVA therapy. Results: Tender joint counts, swollen joint counts, morning stiffness showed significant decrease after BVA therapy. But, as acute inflammatory reactants, ESR showed no significant difference and CRP showed significant increase after BVA therapy. Patient global assessment physician global assessment, and KHAQ index showed significant improvement after BVA therapy. Conclusions: BVA therapy can improve rehabilitation and health-related quality of life RA patients as well as clinical symptom and signs. Further study is required in more population with large scale including acute inflammatory reaction of BVA therapy.

Elevational Distribution of Breeding Bird Communities in Seoraksan National Park, Korea

Hwang, Hyun-Su,Lee, Jae-Kang,Eom, Tae-Kyung,Bae, Ho-Kyoung,Lee, Dong-Ho,Lim, Jong-Hwan,Jung, Sung-Cheol,Park, Chan-Ryul,Rhim, Shin-Jae Korean Society of Forest Science 2020 한국산림과학회지 Vol.109 No.1

In this study, the elevational distribution of breeding birds in Seoraksan National Park, Korea was investigated. Forty-six species of birds were documented from line transect surveys taken from Seorakdong at 230 m above sea level (a.s.l.) to the Daechungbong summit at 1708 m asl. Birdspecies richness and diversity were highest in Seorakdong and lowest at the Daechungbong summit. As elevation increased, bird species richness and diversity decreased, with a humped-shape trend being observed between 700 m and 1200 m a.s.l. Stepwise analyses revealed that breeding bird species diversity indices were significantly negatively correlated with elevation (r² = 0.327, P < 0.001) and positively correlated with vegetational coverage (r² = 0.324, P = 0.046). Higher elevations supported fewer birds than low and intermediate elevations. Projections at the local scale, including data on behavior and habitat use by birds, will be necessary for optimal conservation and management of the bird communities in Seoraksan National Park.

Elevational Distribution of Breeding Bird Communities in Seoraksan National Park, Korea

( Hyun-su Hwang ),( Jae-kang Lee ),( Tae-kyung Eom ),( Ho-kyoung Bae ),( Dong-ho Lee ),( Jong-hwan Lim ),( Sung-cheol Jung ),( Chan-ryul Park ),( Shin-jae Rhim ) 한국산림과학회(구 한국임학회) 2020 한국산림과학회지 Vol.109 No.1

In this study, the elevational distribution of breeding birds in Seoraksan National Park, Korea was investigated. Forty-six species of birds were documented from line transect surveys taken from Seorakdong at 230 m above sea level (a.s.l.) to the Daechungbong summit at 1708 m asl. Birdspecies richness and diversity were highest in Seorakdong and lowest at the Daechungbong summit. As elevation increased, bird species richness and diversity decreased, with a humped-shape trend being observed between 700 m and 1200 m a.s.l. Stepwise analyses revealed that breeding bird species diversity indices were significantly negatively correlated with elevation (r² = 0.327, P < 0.001) and positively correlated with vegetational coverage (r² = 0.324, P = 0.046). Higher elevations supported fewer birds than low and intermediate elevations. Projections at the local scale, including data on behavior and habitat use by birds, will be necessary for optimal conservation and management of the bird communities in Seoraksan National Park.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

WDHA(Watery diarrhea, Hypokalemia, Achlorhydria) syndrome 1예

박기룡,김현영,김지혜,임영찬,최영식,박요한,최경현,이재화,장희경 고신대학교 의학부 2003 高神大學校 醫學部 論文集 Vol.18 No.1

저자들은 부갑상선절제술 이후에도 교정되지 않는 고칼슘혈증과 계속된 수양성 설사로 인한 하지마비 및 전신쇠약을 주소로 내원한 70세 남자환자에서 일반 화학검사, 호르몬 건사, 방사선 검사 등으로 VIPoma와 동반된 MEN Ⅰ으로 진단하여 외과적 절제술 후 수양성설사, 저칼슘혈증 및 고칼슘혈증이 호전된 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Multiple endocrine neoplasia I (MEN Ⅰ) is a genetic disorder that consists of neoplasia of neuroendocrine type in the parathyroid glands, in the islets of Langerhans in the pancreas, and in the anterior pituitary gland. Primary hyperparathyroidism is most common feature of the MEN I and occurs is approximately 95% of patients. Pancreatic islet cell tumors occur in 40% of MEN I patients. Most of these tumors produce excessive amounts of hormone, such as gastrin, insulin, glucagon and vasoactive intestinal polypeptide (VIP). VIP-producing pancreatic tumors (VIPoma) associated with MEN I are rare and there was only one case report in Korea so far. Recently we experienced a case of MEN I, especially VIPoma presented in a 70 year old man. He got subtotal parathyroidectomy for hyperparathyroidism 5 months ago, but his symptoms of watery diarrhea, severe general weakness and paralysis of lower limbs were not improved and hypercalcemia, hypokalemia were persisted. On computerized tomography scan of abdomen, demonstrated a mass of 3㎝ in diameter at the tail of pancreas. Serum level of VIP hormone was 250pg/㎖ (normal range : 0∼23pg/㎖). After partial pancreatectomy, his symptoms were improved dramatically and biochemical parameters such as, serum potassium, calcium, and VIP returned to the normal ranges.

고추재배를 위한 시설하우스 폐양액의 재활용

박창진,양재의,김경희,유경열,옥용식 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

본 연구에서는 시설하우스 폐양액이 고추의 생육과 양분 흡수에 미치는 영향을 평가하고 폐양액 관주시 부가적으로 얻을 수 있는 관개 효과를 산출함으로써 폐양액의 토양 시비에 따른 농업적 가치를 평가하였다. 시설하우스 폐양액의 토양 곽주시 Ca, Mg, K 등 염류의 증가로 인하여 pH와 EC는 상승하였고, 총질소는 암모늄태 질소와 질산태 질소의 증가와 함께 처리 후 100 mg kg^(-1) 이상 증가하였다. 고추 생육은 화학 비료 70% 및 폐양액 30% 혼합처리구와 화학비료 50% 및 폐양액 50% 혼합치리구에서 양호하였으며 양분 흡수도 동일한 경향을 나타내었다. 고추 수확량은 화학비료 70%, 및 폐양액 30% 혼합처리구에서 가장 높은 것으로 조사되었다. 1일 발생되는 폐양액(288 L 10 a^(-1)day^(-1))을 토양에 시용하여 증발산되는 수분을 보충할 경우 최고 409.86 m²의 면적을 관개할 수 있는 것으로 산출되었다. 이상의 결과를 통해 폐양액의 토양 처리는 화학비료 대체 효과와 폐양액의 정화 효과를 동시에 얻을 수 있으며 적정량 사용시 주변 환경에 미치는 영향을 최소화하면서 지속적인 처리가 가능할 것으로 판단되었다. Waste of the hydroponic solution from the plastic film house cultivation was recycled to grow the red pepper(Capsicum annum L.) in upland fields as supplement for plant nutrients and irrigation sources. Application of hydroponic waste solution increased the pH and EC of the soils, coupled with the increases in the concentrations of exchangeable cations(Ca, Mg, and K), total nitrogen, NH₄-N, and NO₃-N. Growth and yield of red pepper were highest when the treatment of chemical fertilizer(70%) was combined with hydroponic waste solution(30%). Amounts of the daily producing hydroponic waste solution were 2,880 L ha^(-1) day^(-1) from the experimental facilities and this could irrigate 409.86 m?of area to compensate for the amount of water loss by evapotranspiration(3%). The overall results demonstrated that hydroponic waste solution could be recycled as plant nutrients and irrigation water resources for enhancing soil fertility and environmental quality.

SEM-EDX를 이용한 대기 점오염에서 배출된 개별입자상 오염물질의 물리화학적 특성

박정호,양수명,강경희,정재우 진주산업대학교 2004 論文集 Vol.43 No.-

The purpose of the study was to classify individual particle emitted from the point source of air pollution emissions in Kyongsangnam-do by SEM/EDX (scanning electron microscopy / energy dispersive X-ray analyzer). The SEM/EDX provided various physical parameters including particle`s optical diameter and chemical information. The total of 344 individual particle sample were collected at 5 point source including coal-fired power plant, incinerator, and oil boiler. Thus number fraction of each particle can be estimated based on chemical composition. To obtain number fraction of each particle class, an agglomerative hierarchical cluster analysis was initially applied to create particle classes for each sample. The study provides opportunities to identify particles source quantitatively and to develope various receptor models.