금화규(Abelmoschus manihot) 꽃 추출물의 In Vitro 피부 미백 및 주름 개선 효능 연구

권현지 ( Hyun-ji Kwon ),범석현 ( Seok-hyun Beom ),현진아 ( Jin-a Hyun ),강은빈 ( Eun-bin Kang ),박하은 ( Ha-eun Park ),한동근 ( Dong-geun Han ),김현정 ( Hyun-jeong Kim ),최은영 ( Eun-young Choi ),안봉전 ( Bong-jeon An ) 대한화장품학회 2021 대한화장품학회지 Vol.47 No.4

본 연구는 화장품 소재로서 금화규(Abelmoschus manihot) 꽃의 미백 활성율 및 항주름 활성을 평가하기 위해 수행하였다. 금화규 꽃은 물과 70% 에탄올을 이용하여 추출하였다. Tyrosinase 억제 활성은 물과 70% 에탄올 추출물 모두 농도 의존적으로 억제 활성이 증가 된다고 평가되었으며, B16F10 세포주에서 생존율에 영향을 미치지 않은 농도인 10, 25, 50, 75, 100 μg/mL에서 멜라닌 생합성을 저해하는 것으로 확인되었다. 금화규 꽃의 항주름 효능으로는 collagenase와 elastase의 활성 저해 능력을 측정한 결과 70% 에탄올 추출물이 물 추출물보다 높은 항주름 활성을 보였다. 본 연구 결과를 통하여 금화규 꽃 추출물은 미백 활성과 항주름 활성을 가지는 기능성 화장품 소재로 사용 가능할 것으로 사료된다. In this study, the skin whitening and anti-wrinkle activity of Abelmoschus manihot (A. manihot) flowers were evaluated as a cosmetic material. A. manihot flowers were extracted using water and 70% ethanol. Tyrosinase inhibitory activity was evaluated to increase concentration-dependent inhibitory activity in both water (AMW) and 70% ethanol extracts (AME), and was found to inhibit melanin biosynthesis at concentrations that did not affect survival in B16F10 cell lines at 10, 25, 50, 75, and 100 μg/mL. As a result of measuring the anti-wrinkle effectiveness of A. manihot flowers, 70% ethanol extract (AME) showed higher anti-wrinkle activity than water extract (AMW). Through the results of this study, it is considered that the A. manihot flower extract can be used as a functional cosmetic material with whitening and anti-wrinkle activity.

소아의 급성 인두염에서 연쇄구균 신속항원검사의 유용성

임기현 ( Ki Hyun Lim ),최우진 ( Woo Jin Choi ),김미진 ( Mi Jin Kim ),김영호 ( Young Ho Kim ),정지아 ( Ji A Jung ),양승 ( Seung Yang ),황일태 ( Il Tae Hwang ),이혜란 ( Hae Ran Lee ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2006 소아알레르기 및 호흡기학회지 Vol.16 No.1

목 적 : 최근 소아 급성 인두염에서 부적절한 항생제 투여가 문제시 되고 있으나 증상만으로 항생제 사용 여부를 결정하기는 어려우며 인두배양검사가 확진 방법이다. 그러나 검사에 시간이 요구되므로 미국소아과학회에서는 배양검사와 신속항원검사를 시행하여 신속항원검사가 음성이면 항생제 사용을 보류하도록 권하고 있다. 저자들은 이와 같은 지침을 국내에서도 적용할 수 있는지 알아보고자 연쇄구균 신속항원검사의 유용성을 조사하였다. 방 법 : 2004년 9월부터 2005년 8월까지 한림대학교 강동성심병원 소아과를 방문하여 급성 인두염으로 진단받은 70명을 대상으로 하였다. 편도 삼출, 발열, 피부 발진 등의 임상적 특징과 내원 전 항생제 투여 여부를 조사하고, CBC, ASO, CRP 등의 혈액 검사와 인두에서 연쇄구균 신속항원검사와 배양검사를 시행하였다. 결 과 : 1) 인두배양검사에서 A군 연쇄구균이 분리된 양성례는 14례(20.0%)이었으며, 연쇄구균 신속항원검사는 22례(31.4%)에서 양성이었다. 인두배양검사에 대한 신속항원검사의 민감도는 78.6 %, 특이도는 80.4%이었다. 양성예측치는 50.0%, 음성예측치는 93.8%이었다. 2) 발열 유무에 따라 인두배양검사와 신속항원검사에는 차이가 없었다. 3) 편도삼출의 여부에 따라 인두배양검사 양성률에 차이가 없었으며, 신속항원검사는 오히려 편도삼출이 없는 경우에서 양성이 더 많았다. 4) 성홍열양 발진이 있는 경우에 인두배양검사와 신속항원검사에서 양성이 많았다. 5) 인두배양검사 양성은 항생제를 이전에 투여한 경우에 35례 중 3례(8.57%) 이전에 항생제를 투여하지 않은 경우의 35례 중 11례(31.43%)로 낮아 항생제 사용이 인두배양검사에 영향을 주었다. 이전에 인두배양검사는 양서d음성이어도 신속항원검사는 양성인 경향이 있었다. 6) 인두배양검사와 신속항원검사에 따라 혈액의 백혈구 수, ASO, CRP 값은 각각 통계학적인 차이가 없었다. 결 론 : 소아 급성 인두염에서 신속항원검사는 연쇄구균 감염의 진단에 유용한 도구로서 항생제의 조기 투여 여부를 결정하는 데 도움이 되리라 사료된다. Purpose : The purpose of this study was to evaluate the utility of rapid antigen detection test for group A streptococci in children with acute pharyngitis. Methods : Seventy children with acute pharyngitis enrolled the study, who visited the Department of Pediatrics, Kangdong Sacred Heart Hospital, Hallym Medical Center from September 2004 to August 2005. The presence of clinical features such as tonsillar exudates, fever, scarletiniform rash and previous antibiotic use were evaluated and throat swab culture, rapid antigen detection test for group A Streptococci, CBC, ASO, and CRP were performed. Results : Among 70 children with acute pharyngitis, the prevalence of group A beta-hemolytic Streptococcus by throat swab culture and rapid antigen detection test was 14(20.0 %) and 22(31.4%), respectively. The sensitivity of the rapid antigen detection test was 78.6 %, specificity was 80.4%, the positive predictive value was 50.0% and the negative predictive value was 93.8%. The positivity of the throat swab culture and rapid antigen detection test showed no signigicant differences according to the presence of the clinical symptoms except scarletiniform rash. Laboratory data such as CBC, ASO or CRP proved not valuable to predict the positivity of the throat swab culture and/or rapid antigen detection test for group A Streptococcus. There was a tendency of negative throat swab culture and positive rapid antigen detection test in cases of previous antibiotic use. Conclusion : The rapid antigen detection test can be a useful diagnostic tool for early detection of the group A Streptococcus in children with acute pharyngitis and can be helpful for the clinician to reduce the inappropriate use of antibiotics. [Pediatr Allergy Respir Dis (Korea) 2006;16:57-65]

Spinach Saponin-Enriched Fraction Inhibits Platelet Aggregation in cAMP- and cGMP-Dependent Manner by Decreasing TXA2 Production and Blood Coagulation

( Hyun Jeong Cho ),( Sun A Choi ),( Chun Gyu Kim ),( Tae Sung Jung ),( Jeong Hwa Hong ),( Man Hee Rhee ),( Hye Jin Park ),( Hwa Jin Park ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.2

In this study, we investigated the effect of spinach saponin-enriched fraction (SSEF) on collagen (10 μg/ml)-stimulated platelet aggregation. SSEF inhibited collagen-induced platelet aggregation, and which was involved in the inhibition of thromboxane A2 (TXA2) production, an intracellular Ca2+ -agonist as an aggregation-inducing autacoidal molecule. In addition, SSEF significantly increased the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), intracellular Ca2+ -antagonists as aggregation-inhibiting molecules, in collagen-stimulated platelets. These results suggest that SSEF might inhibit Ca2+-elevation and TXA2 formation by increasing the production of Ca2+-antagonistic molecules cAMP and cGMP. These mean that SSEF is a potent inhibitor of collagen-stimulated platelet aggregation. On the other hand, prothrombin time (PT) and activated partial thromboplastin time (APTT) were potently prolonged by SSEF. These findings suggest that SSEF prolongs the internal time between the conversion of fibrinogen to fibrin. Accordingly, our data demonstrate that SSEF may be a crucial tool for a negative regulator during platelet activation and blood coagulation on thrombotic diseases.

Molecular and Clinical Features of Fluconazole Non-susceptible Candida albicans Bloodstream Isolates Recovered in Korean Multicenter Surveillance Studies

Choi Min Ji,Kwon Yong Jun,Byun Seung A,Kim Mi-Na,Lee Wee Gyo,Lee Jaehyeon,Yong Dongeun,Chang Chulhun L.,Won Eun Jeong,Kim Soo Hyun,이승엽,신종희 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.6

Acquired fluconazole resistance (FR) in bloodstream infection (BSI) isolates of Candida albicans is rare. We investigated the FR mechanisms and clinical features of 14 fluconazole non-susceptible (FNS; FR and fluconazole-susceptible dose-dependent) BSI isolates of C. albicans recovered from Korean multicenter surveillance studies during 2006–2021. Mutations causing amino acid substitutions (AASs) in the drug-target gene ERG11 and the FR-associated transcription factor genes TAC1, MRR1, and UPC2 of the 14 FNS isolates were compared with those of 12 fluconazole-susceptible isolates. Of the 14 FNS isolates, eight and seven had Erg11p (K143R, F145L, or G464S) and Tac1p (T225A, R673L, A736T, or A736V) AASs, respectively, which were previously described in FR isolates. Novel Erg11p, Tac1p, and Mrr1p AASs were observed in two, four, and one FNS isolates, respectively. Combined Erg11p and Tac1p AASs were observed in seven FNS isolates. None of the FR-associated Upc2p AASs were detected. Of the 14 patients, only one had previous azole exposure, and the 30-day mortality rate was 57.1% (8/14). Our data show that Erg11p and Tac1p AASs are likely to contribute to FR in C. albicans BSI isolates in Korea and that most FNS C. albicans BSIs develop without azole exposure.

Sequence Level Analysis of Recently Duplicated Regions in Soybean [ <i>Glycine max</i> (L.) Merr.] Genome

Van, Kyujung,Kim, Dong Hyun,Cai, Chun Mei,Kim, Moon Young,Shin, Jin Hee,Graham, Michelle A.,Shoemaker, Randy C.,Choi, Beom-Soon,Yang, Tae-Jin,Lee, Suk-Ha Oxford University Press 2008 DNA research Vol.15 No.2

<P>A single recessive gene, <I>rxp</I>, on linkage group (LG) D2 controls bacterial leaf-pustule resistance in soybean. We identified two homoeologous contigs (GmA and GmA′) composed of five bacterial artificial chromosomes (BACs) during the selection of BAC clones around <I>Rxp</I> region. With the recombinant inbred line population from the cross of Pureunkong and Jinpumkong 2, single-nucleotide polymorphism and simple sequence repeat marker genotyping were able to locate GmA′ on LG A1. On the basis of information in the Soybean Breeders Toolbox and our results, parts of LG A1 and LG D2 share duplicated regions. Alignment and annotation revealed that many homoeologous regions contained kinases and proteins related to signal transduction pathway. Interestingly, inserted sequences from GmA and GmA′ had homology with transposase and integrase. Estimation of evolutionary events revealed that speciation of soybean from <I>Medicago</I> and the recent divergence of two soybean homoeologous regions occurred at 60 and 12 million years ago, respectively. Distribution of synonymous substitution patterns, <I>K</I><SUB>s</SUB>, yielded a first secondary peak (mode <I>K</I><SUB>s</SUB> = 0.10–0.15) followed by two smaller bulges were displayed between soybean homologous regions. Thus, diploidized paleopolyploidy of soybean genome was again supported by our study.</P>

Korean Ginseng Berry Polysaccharide Enhances Immunomodulation Activities of Peritoneal Macrophages in Mice with Cyclophosphamide-Induced Immunosuppression

Choi JeongUn,Nam Ju Hyun,Rod-in Weerawan,Monmai Chaiwat,Jang A-yeong,You SangGuan,Park Woo Jung 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.6

Korean ginseng (Panax ginseng C. A. Meyer), a member of the Araliaceae family, is known as a traditional medicinal plant to have a wide range of health properties. Polysaccharides constitute a major component of Korean ginseng, and its berries exhibit immune-modulating properties. The purpose of this study was to investigate the immune effects of crude polysaccharide (GBPC) extracted from Korean ginseng berry on peritoneal macrophages in mice with cyclophosphamide (CY)- induced immunosuppression. BALB/c mice were divided into eight groups: normal control, normal control + CY, levamisole + CY, ginseng + CY, and four concentrations of 50, 100, 250, and 500 mg/kg BW/day of GBPC + CY. Mice were orally administered with samples for 10 days. Immunosuppression was established by treating mice with CY (80 mg/kg BW/day) through intraperitoneal injection on days 4 to 6. The immune function of peritoneal macrophages was then evaluated. Oral administration of 500 mg/kg BW/day GBPC resulted in proliferation, NO production, and phagocytosis at 100%, 88%, and 91%, respectively, close to the levels of the normal group (100%) of peritoneal macrophages. In CY-treated mice, GBPC of 50−500 mg/kg BW/day also dosedependently stimulated the proliferation, NO production, and phagocytosis at 56−100%, 47−88%, and 53−91%, respectively, with expression levels of immune-associated genes, such as iNOS, COX−2, IL−1β, IL−6, and TNF–α, of about 0.32 to 2.87-fold, compared to those in the CY group. GBPC could be a potential immunomodulatory material to control peritoneal macrophages under an immunosuppressive condition.

Inappropriate Gonadotropin Secretion in Polycystic Ovary Syndrome: The Relationship with Clinical, Hormonal and Metabolic Characteristics

( A Ra Shim ),( Yu Im Hwang ),( Kyung Jin Lim ),( Young Mi Choi ),( Young Eun Jeon ),( Seok Kyo Seo ),( Si Hyun Cho ),( Young Sik Choi ),( Byung Seok Lee ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.11

Objective To evaluate the clinical, hormonal and metabolic characteristics according to luteinizing hormone (LH)/follicle stimulating hormone (FSH) ratio in women with polycystic ovary syndrome (PCOS). Methods A total of 225 women with PCOS were included in this study. They were divided into two groups according to LH/FSH ratio; group A (LH/FSH < 2, n=160) and group B (LH/FSH > 2, n=65). We compared clinical, hormonal and metabolic characteristics including age at menarche, body mass index (BMI), blood pressures, ovarian volume, and serum androgen levels between the two groups. Serum glucose and insulin levels, fasting glucose/insulin ratio, homeostatic model assessment of insulin resistance (HOMA-IR), and lipid profi les were also compared between the two groups. Pearson correlation coeffi cients were used to evaluate correlation between the LH/FSH ratio and various parameters. Results Age of menarche in the group B was signifi cantly later than that in the group A. BMI, waist-hip ratio and blood pressures were signifi cantly lower in the group B compared to those of the group A. Ovarian volume and serum levels of LH, estradiol, total testosterone, sex-hormone binding globulin, 17-hydroxyprogesterone (OHP), and high density lipoprotein were signifi cantly higher in the group B. Postprandial 2 hours glucose, insulin and HOMA-IR were signifi cantly higher in the group A. After adjustment of BMI, the LH/FSH ratio was signifi cantly positively correlated with age at menarche, ovarian volume, total testosterone levels, and 17-OHP levels. Conclusion The inappropriate gonadotropin secretion may be negatively correlated with BMI, and positively with age of menarche and increased ovarian androgen production irrespective of BMI in women with PCOS. However, it may not be associated with metabolic characteristics.

Transgenic Cucumber Expressing Nit Gene

Hyun A Jang,Ga Min Lim,Hyun A Kim,Youn-il Park,Suk Yoon Kwon,Pil Son Choi 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

To produce abiotic stress resistant transgenic cucumber, the cotyledonary node explants of cucumber (c.v. Eunsung) were inoculated with A. tumefaciens strain EHA105 containing the binary vector (pPZP211) carrying Nit gene. The 491 explants inoculated with bacterium solution for 30 min were maintained on 50 mg/L paromomycin contained shoot induction (SI) medium for first 2 weeks and then subcultured on 100 mg/L paromomycin to obtain transgenic adventitious shoots for 4 x 14 days. So far, 5 plant were selected, and then acclimated in soil. Of them, 3 transgenic plants with Nit gene were confirmed by Southern blot analysis.

아그로박테리움을 이용한 Actinobacillus pleuropneumoniae ApxIIA (ApxII toxin) 유전자 발현 옥수수 형질전환체 개발

김현아,유한상,양문식,권석윤,김진석,최필선,Kim, Hyun-A,Yoo, Han-Sang,Yang, Moon-Sik,Kwon, Suk-Yoon,Kim, Jin-Seog,Choi, Pil-Son 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.3

To develop edible vaccines for swine, the embryogenic calli (type II) derived from HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vector pMYV611, 613, 616, and V621, 622 and 623 respectively. Six of those vectors carry nptII gene which confers resistance to paromomycin and apxIIA gene producing ApxII toxin which is generated in various serum types of A. pleuropneumoniae as a target gene. The 4,120 callus clones for pMYV611, 5,959 callus clones for pMYV613, 7,581 callus clones for pMYV616, 52,329 callus clones for V621, 48,948 callus clones for V622, and 56,188 callus clones for V623 were inoculated. The frequency of positive response clone was confirmed into range of 2.3% - 4.4% for each vectors by NPTII ELISA kit assay, and the selected callus clones of them were finally 3 callus clones from pMYV611 (0.07%), 4 callus clones from pMYV613 (0.07%), 2 callus clones from pMYV616 (0.03%), 51 callus clones from V621 (0.1%), 72 callus clones from V622 (0.15%), and 102 callus clones from V623 (0.18%) respectively. From the selected callus clones of each binary vector, the integration of the apxIIA gene into maize genome was detected from 2 plants of pMYV613 and 2 plants of V623 by Southern blot analysis. 돼지 흉막폐렴백신을 개발하기 위해 옥수수 HiII genotype 으로부터 유도한 type II형의 배발생캘러스를 식물발현벡터 pMYV611, pMYV613, pMYV616, V621, V622 및 V623로 형질전환시킨 Agrobacterium (C58C1)과 공동배양 하였다. 이들 식물발현벡터는 paromomycin 항생제 저항 유전자인 NPTII 선발마커와 표적 유전자로서 흉막폐렴균의 여러 가지 혈청을 생산하는 apxIIA유전자로 재조합하여 구축하였다. 식물발현벡터pMYV611, pMYV613, pMYV616, V621, V622 및V623의 경우 각각 4,120개, 5,959개, 7,581개, 52,329개, 48,948개 및 56,188개의 캘러스 클론을 Agrobacterium과 공동한 후 NPTII assay kit에 의해 nptII유전자의 발현빈도를 조사한 결과 각 벡터별로 2.3-4.4%의 캘러스 클론에서 항체결합 양성반응을 보였고, 이들 중 최종적으로 선발된 형질전환 캘러스 클론은 pMYV611에서 3개 (0.07%), pMYV613에서 4개 (0.07%), pMYV616에서 2개 (0.02%), V621에서 51개 (0.1%), V622에서 72개 (0.15%) 및 V623에서 102개 (0.18%)를 각각 얻었다. 형질전환된 캘러스 클론으로부터 재분화된 식물체에서 유전자 도입여부를 Southern 분석으로 통해 확인한 결과 pMYV613에서 2개 식물체 및 V623에서 얻은 2개 식물체에서 각각 확인되었다.

Comparative Evaluation of Antioxidant, Anti-diabetic and Anti-diabetic Complication Effects of Artemisia capillaris Thunb, A. capillaris Herba and A. iwayomogi

A Ra Jo,Eun Bi Kuk,Seo in Oh,Hee Sook Sohn,Jae Sue Choi,Hyun Ah Jung 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8