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P-14 : Development of Real Time PCR Assay for Detection of Influenza A Virus
( Hye Soon Seong ),( Lyong Hyo Kim ),( Su Jin Jeong ),( En Mi Je ),( Kyung Ok Lee ),( Min Young Park ) 대한임상병리사협회 2009 임상미생물검사학회 발표자료집 Vol.2009 No.-
Background: Accurate and rapid diagnosis of influenza A virus infection through timely implementation of antiviral treatment and other public health based measures is critical for minimizing further spread. The aim of this study was to develop an influenza A real time PCR assay with CDC (Centers of Disease Control and Prevention, USA) protocol and also to evaluate it for clinical application. Methods: Sample types tested included flocked nasopharyngeal (NP) swabs (Copan, Murrieta, CA) submitted in universal transport media (UTM, DHI), NP aspirates and NP washes. We used the primer and probe sequences of CDC protocol verified by WHO for detection of influenza A virus (protocol reference: I-007-005, version 2009 swine influenza). This assay was compared with the commercial kit (Roche, Germany). Results: In the present study, a real time PCR assay was developed and influenza A was successfully detected in clinical specimens and in the control group (ATCC VR546). This assay showed a good correlation with the commercial Roche kit. Conclusion: Recently a novel influenza A virus, subtype H1N1, has been spreading rapidly to many regions of the world. Influenza A RNA must be detected in patient samples for the final diagnosis of novel influenza (H1N1) A infection. Based on these results, we believe that this molecular test can perform an important role as a sentinel test to detect influenza A viruses in patients presenting influenza-like illness (ILI) and therefore act as an early warning system for the detection of future pandemic influenza threats.
( Hye Soon Seong ),( Mi Ok Park ),( Tae Young Cho ),( Mi Sun Jang ) 대한임상병리사협회 2005 조직세포검사학회 발표자료집 Vol.2004 No.-
Background Human papillomaviruses (HPVs) are a group of more than 100 types of viruses. Of the more than 100 types of HPVs, over 30 types can be passed from one person to another through sexual contact. HPVs are now recognized as the major cause of cervical cancer. HPV infected populations have more higher probability than populations absence of HPV in cervical disease, also. So far, Pap smear had been used as earlier diagnosis in cervical cancer. But, other method need as public prosecutor because false negative ratio(5~66%) is high. Moreover, it is impossible that the detection of latency of HPVs by Pap smear. In this study, we attempted to estimate the extent of HPV infection using HPV DNA Chip test at normal limits Pap smear(Class I, II) Method From August 2003 to February 2004, 2,577 samples for routine Pap smear screening were requested to NeoDin Medical Institute. Among 2,577 samples, 499 samples showed within class I of cytologic smear and 2,078 samples showed with class II. All specimens were detected and typed for HPV using DNA chip(Biomedlab Co., Korea) and followed the manufacturer``s protocol. HPV L1 gene fragment(150bp) was PCR amplified for the samples with positive β-globin amplification(200bp). After PCR amplification and hybridization, 22 HPV types were determined in clinical specimens with fluorescence scan for hybridization signals at laser power. Results In 115 samples(23.0%) of the 499 samples class I group, HPV DNAs were detected.(Table 1). The duplex high-risks were present in 63 samples(49.6%), low-risks found in 64 samples(29.9%), other types found in 26 samples(20.5%) and multi-infections found in 12 samples(10.4%). On the other hand, Class II group was showed HPV DNAs in 474 samples(22.8%) of 2,078 samples(Table 1). The positive ratio was similar of class I group. But infection value was higher than class I group in high-risk groups; high-risks found in 404 samples(72.3%), low-risks found in 155 samples(19.0%), other types found in 60 samples(10.7%) and multi-infections found in 85 samples(18%). Table 1. Prevalent types of HPV positive in class I and class II * : PCR(+), HPV Chip(-) Conclusions In order to estimate HPV infection at normal Pap smear, HPV DNA Chip test was performed. Following our data, the population with normal limits of Pap smear showed positive predictive value(class I : 23.0%, class II : 22.8%) of HPV infection. We recommend that population with cytologic normal limits should perform HPV genotyping and Pap smear, simultaneously
The Extract of Herbal Medicines Activates AMP-Activated Protein Kinase in Diet-Induced Obese Rats
Shin, Hye-Yeon,Chung, SaeYeon,Kim, Soon Re,Lee, Ji-Hye,Seo, Hye-Sook,Shin, Yong-Cheol,Ko, Seong-Gyu Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P>Our study investigated whether the extract of six herbal medicines (OB-1) has an inhibitory effect on obesity. High-fat diet-(HFD-) induced rats and controls were treated with 40 mg/100 g body weight of OB-1 or saline once a day for 5 weeks. After significant changes in body weight were induced, OB-1 and saline were administered to each subgroup of HFD and control groups for additional 5 weeks. No statistically significant decrease of body weight in OB-1-treated rats was found compared to controls. However, OB-1-treated rats were found to be more active in an open-field test and have a reduction in the size of adipocytes compared to controls. We observed no changes in the mRNA expressions of leptin and adiponectin from adipocytes between OB-1- and saline-treated rats with HFD-induced obesity group. However, OB-1 treatments were shown to be inversely correlated with accumulation of lipid droplets in liver tissue, suggesting that OB-1 could inhibit a lipid accumulation by blocking the pathway related to lipid metabolism. Moreover, the phosphorylation of AMP-activated protein kinase (AMPK) was significantly increased in OB-1-treated rats with HFD compared to controls. These results suggest that OB-1 has no direct antiobesity effect and, however, could be a regulator of cellular metabolism.</P>
KIM, SEONG-KYU,KIM, SEONG-HO,NAH, SEONG-SU,LEE, JI HYUN,HONG, SEUNG-JAE,KIM, HYUN-SOOK,LEE, HYE-SOON,KIM, HYOUN AH,JOUNG, CHUNG-IL,BAE, JISUK,CHOE, JUNG-YOON,LEE, SHIN-SEOK Journal of Rheumatology 2013 The Journal of rheumatology Vol.40 No.3
<P><B>Objective.</B></P><P>Guanosine triphosphate cyclohydrolase 1 (GCH1) is the rate-limiting enzyme in the synthesis of tetrahydrobiopterin, which is an essential cofactor in nitric oxide (NO) production. Polymorphisms in the <I>GCH1</I> gene have been implicated in protection against pain sensitivity. The aim of our study was to determine whether single-nucleotide polymorphisms (SNP) in the <I>GCH1</I> gene affect susceptibility and/or pain sensitivity in fibromyalgia syndrome (FM).</P><P><B>Methods.</B></P><P>A total of 409 patients with FM and 422 controls were enrolled. The alleles and genotypes at 4 positions [rs3783641(T>A), rs841(C>T), rs752688(C>T), and rs4411417(T>C)] in the <I>GCH1</I> gene were analyzed. The associations of the <I>GCH1</I> SNP with susceptibility and clinical measures in patients with FM were assessed.</P><P><B>Results.</B></P><P>The frequencies of alleles and genotypes of the 4 SNP did not differ between patients with FM and healthy controls. Among 13 constructed haplotypes, we further examined 4 (CCTT, TTCT, TTCA, and CCTA) with > 1% frequency in both FM and controls. No associations of <I>GCH1</I> polymorphisms with FM-related activity or severity indexes were found, although the number and total score of tender points in patients with FM differed among the 4 haplotypes (p = 0.03 and p = 0.01, respectively). The CCTA haplotype of <I>GCH1</I> was associated with significantly lower pain sensitivity and occurred less frequently than the CCTT haplotype in patients with FM (p = 0.04, OR 0.45, 95% CI 0.21–0.96).</P><P><B>Conclusion.</B></P><P>Our study provides evidence that certain <I>GCH1</I> haplotypes may be protective against susceptibility and pain sensitivity in FM. Our data suggest that NO is responsible for pain sensitivity in the pathogenesis of FM.</P>