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Dexamethasone으로 면역기능(免疫機能) 저하(低下)된 토끼에 Infectious Bovine Rhinotracheitis Virus 감염시험(感染試驗)
민원기,전무형,박성국,안수환,차연호,Min, Won-gi,Jun, Moo-hyung,Park, Seong-kuk,An, Soo-hwan,Cha, Yeon-ho 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1
To establish a laboratory animal model for study on development of diagnostic methods for infectious bovine rhinotracheitis virus(IBRV), experimental infection of the virus to rabbits immunosuppressed with dexamethasone(DX) were carried out. Results obtained throughout the experiments were as follows. When lymphocyte activity was measured by lymphocyte transformation to phytohaemagglutinin in parallel with total and differential leucocyte counting, both groups treated with 2.0mg DX once and 1.0mg DX daily showed marked immunosuppression between 5 to 72 hrs. after administration. The degree of suppression of lymphocyte activities was more remarkable in the latter group. IBRV PQ7 strain at $10^{7.5}\;TCID_{50}/0.2ml$ was inoculated into conjunctival sacs of rabbits immunosuppressed with DX and non-treated. During 3 weeks observation, the immunosuppressed groups revealed mild conjunctivitis, viremia and virus recovery by 33.3 to 100%, whereas the DX nontreated group showed viremia and virus recovery with no clinical conjunctivitis by one of three rabbits(33.3%). In conclusion, it was indicated that experimental infection of IBRV PQ7 strain to rabbit was limited in prerequisite to immunologic modification by administration of immunosuppressive drugs.
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국내에서 분리된 Infectious Bovine Rhinotracheitis Virus DNA의 제한효소분석
민원기,전무형,이중복,김병한,안수환 대한바이러스학회 1989 Journal of Bacteriology and Virology Vol.19 No.1
Nine strains of infectious bovine rhinotracheitis virus(IBRV) isolated in Korea since 1970 were investigated to elucidate the pathogenicity on various cell lines and the reactivity in cross serum neutralization test. The genomes of the viruses were also studied by restriction endonuclease analysis to examine the genetical patterns and the origin of the viruses. Results obtained by experiment were as follows ; 1. In pathogenicity test on various cell lines by cytopathology and immunofluorescence antibody assay, MDBK cells were found to be very susceptible to all of the domestic isolates, Colorado and Oxford strains and RK-13 cells, mildly reactive to all of viruses. However, CV-1 and BHK cells revealed negligible reactions against all of viruses tested. 2. In cross serum neutralization(SN) test with the positive serurn from the csttle immunized with PQ7 strain of IBRV, PQ7, SQ, VS, A14, TSV and S'74-7 strains showed 64 of SN titer, and IQ, QW, A37, Colorado and Oxford strains, 128 of SN titer. It was assumed that there is no difference in major antigenic determinants among the viruses. 3. By analysis of restriction endonuclease Hind lll, it was found that 8 domestic isolates except A37 and Colorado strain revealed the identical banding patterns. However, Oxford strain showed quite different patterns, displaying H band at 6.9x10' daltons and K' bsnd at 4.5x10' daltons. A37 strain exclusively contained the longer bands of A' and A. 4. When the viral DNA were analyzed by restriction endonuclease EcoRI, all of the domestic isolates and Colorado strain showed very similar banding patterns, whereas Oxford strain revealed a considerable discrepancy, displaying B' band at 12.5 x 10' daltons. 5. By analysis of restrietion endonuclease BamHI, it was found that the domestic isolates and Colorado strain revealed similar patterns displaying 8 fragments. However, Oxford strain was cleaved into 9 fragrnents displaying G' and G bands.
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마이크로네시아 연방 축 라군의 해초지와 맹그로브 군락 퇴적물에 서식하는 중형저서동물 군집 특성
민원기,김동성,권문상 한국해양과학기술원 2006 Ocean and Polar Research Vol.28 No.1
This study was conducted to investigate the community structure and distributional pattern of meiobenthos on the sediment of the mangrove forest and seagrass bed in the Chuuk lagoon. The samples were collected by an acryl corer at 14 stations. Nematodes were the most abundant meiobenthos, followed by ciliophorans and polychaetes; these taxa comprised more than 70% of the total abundance at all stations. The meiofauna sampled in seagrass bed were more diverse than those of mangrove substrates. Total densities were higher in mangrove stations than other sites, averaging 1,671 to 2,967 inds./10cm2. Densities in seagrass area ranged between 605 and 1,053 inds./10cm2. Biomasses, however, were higher in seagrass bed (975-2,167mg free dry weight/10cm2) than in mangrove area (1,064-1,180mg free dry weight/10cm2). Ordination chart by MDS of major meiofaunal density in each station showed difference between mangrove area and seagrass area in terms of habitat of meiobenthos.
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