Investigation on the interactions of scutellarin and scutellarein with bovine serum albumin using spectroscopic and molecular docking techniques

Hao Tang,Nian-Guang Li,Zhi-Hao Shi,Yu-Ping Tang,Qian-Ping Shi,Ze-Xi Dong,Peng-Xuan Zhang,Jin-ao Duan 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

The binding abilities of scutellarin (Scu) andscutellarein (Scue) with bovine serum albumin (BSA) wereinvestigated using equilibrium dialysis, high performanceliquid chromatography, fluorescence spectroscopy, competitivesite marker and molecular docking. The resultsshowed that the average protein binding ratios of Scu andScue with BSA were (79.85 ± 1.83) and (85.49 ± 1.21) %respectively. Under simulated physiological conditions, thefluorescence data indicated that Scu and Scue bound withBSA through a static mechanism. The thermodynamicparameters indicated that the interactions of Scu-BSA andScue-BSA mainly occurred by van der Waals forces andhydrogen bonds and it was easier for Scue to bind withBSA than Scu, indicating that the glucuronic acid moleculein Scu decreased the binding affinity. Site competitivemarker experiments showed that the binding sites of Scuand Scue mainly located within the sub-domain IIA ofBSA. Furthermore, molecular docking studies indicatedthat one BSA could bind three Scue, while one BSA couldcarry only two Scu. All these results clearly indicated theinteractions of Scu and Scue with BSA, which will lay thefoundation for further research to determine the pharmacologyand pharmacodynamics of Scu and Scue for treatingischemic cerebrovascular disease.

Effects of selenium source and level on the physiological response, reproductive performance, serum Se level and milk composition in gestating sows

Jin, Xing Hao,Kim, Cheon Soo,Gim, Min Jin,Kim, Yoo Yong Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.12

Objective: This study was conducted to evaluate the effects of selenium (Se) source and level on the physiological response, reproductive performance, serum Se level, and milk composition in gestating sows. Methods: A total of 54 multiparous sows (Yorkshire×Landrace) with average body weight (BW), backfat thickness (BF), and parity were assigned to one of five treatments with 10 or 11 sows per treatment using a 2×2 factorial arrangement with one additional treatment in a completely randomized design. Inorganic or organic Se (IS or OS) sources were added to the diet at 0.30 ppm and 0.50 ppm Se. A non-Se-fortified corn-soybean meal basal diet served as a negative control. Treatments were as follows: i) Control: corn-soybean based diet, ii) IS30: control+inorganic Se 0.30 ppm, iii) IS50: control+inorganic Se 0.50 ppm, iv) OS30: control+ organic Se ppm, and v) OS50: control+organic Se 0.50 ppm. Results: At day 21 of lactation, piglet weight and weight gain in the OS treatments were higher than those in the IS treatments (p<0.05). Meanwhile, adding 0.5 ppm Se also resulted in the same significant differences in piglet BW and weight gain (p<0.05). Colostrum and milk Se concentrations increased (p<0.05) with Se level for both Se sources but were greater when sows were fed organic Se (p<0.05). Except for 24 hours postpartum, the Se concentrations were higher when sows were fed organic Se (p<0.05). Sow serum Se content was greater as Se levels increased from 0.3 ppm to 0.5 ppm at day 110 of gestation, 24 hours postpartum and day 21 of lactation (p<0.05). The pig serum Se concentration increased as the dietary Se level increased (p<0.05) and was higher when the sow dietary Se source was organic (p<0.05). Organic Se 0.5 ppm also had the highest serum Se level at two measured points (p<0.05). Conclusion: Consequently, supplementation with organic Se or 0.5 ppm Se in a gestating diet could improve piglet performance, the Se status of sows and piglets and milk composition, but organic Se at 0.5 ppm is optimal.

Distributed Fusion Filter for Multi-rate Sampling Stochastic Singular Systems with Multiplicative Noises

Hao Jin,Jing Ma,Yun Li,Ming Zhao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2

The distributed fusion filtering problem is studied for multi-rate sampling stochastic singular linear systems with multiple sensors and stochastic multiplicative noises. The system is described at the highest sampling rate and different sensors may have different lower sampling rates. The white noise in measurement matrix is introduced to describe the stochastic disturbance. Firstly, based on decomposition in canonical form, the original singular system is transformed into fast and slow two subsystems. For the two reduced-order subsystems, the local filters (LFs) are given based on the “dummy” random variables. The cross-covariance matrices between any two local filtering errors are derived. Further, the distributed fusion filter weighted by matrices (FFWM) is obtained for the original singular system based on the well-known fusion algorithm in the linear minimum variance sense. Simulation example verifies the correctness and feasibility of the proposed algorithm.

Nondestructive Assessment of Glycosaminoglycans in Engineered Cartilages Using Hexabrix-Enhanced Micro-Computed Tomography

Jin, Long Hao,Choi, Byung Hyune,Kim, Young Jick,Oh, Hyun Ju,Kim, Byoung Ju,Yin, Xiang Yun,Min, Byoung-Hyun 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.11 No.4

Relationship between Invert-Filling Disengaging and Deformation of Shield Tunnel Using Staggered Assembled Segment

Hao Jin,Jie Su,Chen Zhao 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4

The disengaging between invert filling and segment affects the safety of train operation. A 3D numerical model of a three-ring staggered assembled shield tunnel is established, which is verified by a full-scale test. On this basis, cohesive element is inserted between the invert filling and tunnel segment to simulate the fracture process of invert filling-segment interface, and explore the relationship between the deformation of staggered assembled shield tunnel and the disengaging of invert filling. The results show that: 1) The development of invert filling disengaging affects the internal forces of shield tunnel segments. 2) The relationship between the development of invert filling disengaging and the deformation of shield tunnel can be four stages. Different segment assembly methods do not affect the variability of the disengaging ratio. Tensile failure occurs in 89% of the interface layer, which is the main form of invert filling disengaging. 3) The relationship between the development of disengaging distance and the deformation of shield tunnel also can be four stages. The faster the horizontal convergent deformation develops in the segment assembly method, the slower the growth rate of the disengaging distance. 4) The faster the horizontal convergent deformation develops in the segment assembly method, the slower the growth rate of the disengaging volume.

Poster Session : PS 0883 ; Lower GI Tract : Isoliquiritigenin Inhibits TNF-a-Induced HMGB1 Release and HMGB1-Dependent Infl ammation in Ht-29 Cells

( Jin Hua Chi ),( Hao Jin ),( Wen Yi Jiang ),( Sung Hee Lee ),( In Tae Hwang ),( Suck Chei Choi ),( Geom Seog Seo ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: High Mobility Group Box 1 protein (HMGB1) is a chromatin binding nucleus protein and has proinflammatory cytokine potential when released by damaged or necrosis cells during Inflammatory bowel dieases(IBD). This study is aimed to explore the association between ISQ and HMGB1 release in human intestinal cell. Methods: The protein expression of COX-2, fracnation of NF-κB and HMGB1, concentration of culture medium were analyzed by Western blot. Translocation of NF-κ B and HMGB1 were assessed by Fluorescence staining. Co-Immunoprecipitation assay for demonstrated acetyled HMGB1 in medium. HMGB1 and COX-2 mRNA level was analyzed by RT-PCR. Results: ISQ reduce TNF-a induced release of HMGB1 in extracellular and inhibit nucleus/cytosol translocation. ISQ also regulates NF-κB p65 translocation and inhibit the COX-2 expression which is the downstream of the NF-κB. Moreover ISQ suppressed the release of HMGB1 through reduction of the mRNA level and inhibit HMGB1 acetylation. Conclusions: In this study, all data evidence that released HMGB1 is a proinflammatory cytokine and leads to signaling cascades in inflammatory responses in human intestinal cell. These findings highlight the potential of ISQ for clinical applications in the treatment of intestinal inflammation including IBD.

An Empirical Analysis on the Transfer of Agricultural Labor Surplus and the Farmers' Income Growth in China

Hao Jin,Su-Ying Gao,Ki-Woong Lee,Kyung-Hwan Song 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

Synthesis and characterization of cholic acid-containing biodegradable hydrogels by photoinduced copolymerization

Hao, Jin-Qing,Li, Hong,Woo, Hee-Gweon Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of applied polymer science Vol.112 No.5

<P>A cholic acid (CA)-containing biodegradable hydrogel (PLA-PEG-PLA-co-MACAH) was synthesized from the photoinduced copolymerization of a CA-modified methacrylate monomer (MACAH), bearing a spacer of hexane-1,6-diol spacer between the methacryloyl and the cholanoate moieties, and a macromonomer (PLA-PEG-PLA-DA), bearing two acryloyl end groups derived from a poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) triblock copolymer. The structure of MACAH was confirmed by FTIR, <SUP>1</SUP>H-NMR, and MS. The hydrogel PLA-PEG-PLA-co-MACAH was characterized by scanning electron microscopy and X-ray diffraction. The experiment results showed that the swelling ratios of the hydrogels decreased with the increase of the CA fraction. The investigation on the in vitro degradation of the hydrogel showed that the CA-containing hydrogels degraded much slower than the hydrogels without CA component. The bioactivity of the synthesized hydrogels was assessed by the simulated body fluid method. The observed formation of hydroxyapatite on the scaffold of the hydrogels indicated that the hydrogels possess good bioactivity. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009</P>

GRIM-19 Expression and Function in Human Gliomas

Jin, Yong-Hao,Jung, Shin,Jin, Shu-Guang,Jung, Tae-Young,Moon, Kyung-Sub,Kim, In-Young The Korean Neurosurgical Society 2010 Journal of Korean neurosurgical society Vol.48 No.1

Objective : We determined whether the expression of GRIM-19 is correlated with pathologic types and malignant grades in gliomas, and determined the function of GRIM-19 in human gliomas. Methods : Tumor tissues were isolated and frozen at $-80^{\circ}C$ just after surgery. The tissues consisted of normal brain tissue (4), astrocytomas (2), anaplastic astrocytomas (2), oligodendrogliomas (13), anaplastic oligodendrogliomas (11), and glioblastomas (16). To profile tumor-related genes, we applied RNA differential display using a $Genefishing^{TM}$ DEG kit, and validated the tumor-related genes by reverse transcription polymerase chain reaction (RT-PCR). A human glioblastoma cell line (U343MG-A) was used for the GRIM-19 functional studies. The morphologic and cytoskeletal changes were examined via light and confocal microscopy. The migratory and invasive abilities were investigated by the simple scratch technique and Matrigel assay. The antiproliferative activity was determined by thiazolyl blue Tetrazolium bromide (MTT) assay and FACS analysis. Results : Based on RT-PCR analysis, the expression of GRIM-19 was higher in astrocytic tumors than oligodendroglial tumors. The expression of GRIM-19 was higher in high-grade tumors than low-grade tumors or normal brain tissue; glioblastomas showed the highest expression. After transfection of GRIM-19 into U343MG-A, the morphology of the sense-transfection cells became larger and more spindly. The antisensetransfection cells became smaller and rounder compared with wild type U343MG-A. The MTT assay showed that the sense-transfection cells were more sensitive to the combination of interferon-$\beta$ and retinoic acid than U343MG-A cells or antisense-transfection cells; the antiproliferative activity was related to apoptosis. Conclusion : GRIM-19 may be one of the gene profiles which regulate cell death via apoptosis in human gliomas.

Probiotic Properties of Pediococcus acidilactici M76 with Functional Exopolysaccharides and Its Lactic Acid Fermentation of Black Raspberry Extract

Hao Jin,Young-Ran Song,Chan-Mi Lee,Seon-Hye Lee,Sang-Ho Baik 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Pediococcus acidilactici M76 (PA-M76) showed outstanding probiotic properties with high tolerance in acidic GIT environments, broad antimicrobial activity, and high adhesion capability in the intestinal tract of Caenorhabditis elegans. PA-M76 treatment resulted in significant increases of pro-inflammatory cytokine mRNA expression in macrophages. PA-M76 produced 1.62 g/L EPS under optimal lactic acid fermentation conditions. Lactic acid fermentation of BRE by PA-M76 did not significantly affect the total anthocyanin and flavonoid content, except for a significant increase in total polyphenol content compared to non-fermented BRE (NfBRE). However, fBRE exhibited increased DPPH radical scavenging activity, linoleic acid peroxidation inhibition rate, and ABTS scavenging activity of fBRE compared to NfBRE. Among the 28 compounds identified in the GC-MS analysis, esters were present as the major groups. The total concentration of volatile compounds was higher in fBRE than that in NfBRE. However, the undesirable flavor of terpenes decreased. PA-M76 might be useful for preparing functionally enhanced fermented beverages with a higher antioxidant activity of EPS and enhanced flavors.