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Kim ,, Bong‐,Gyu,Jung ,, Na Ri,Joe, Eun Ji,Hur, Hor‐,Gil,Lim, Yoongho,Chong, Youhoon,Ahn, Joong‐,Hoon WILEY‐VCH Verlag 2010 Chembiochem Vol.11 No.17
<P><B>Just add sugar</B>: An unnatural flavonoid‐deoxyaminosugar conjugate was biologically synthesized in <I>Escherichia coli</I> by engineering an <I>E. coli</I> nucleotide–sugar pathway and expressing a glycosyltrandfease specific for UDP‐arabinose derivatives. </P>
Kim Gyu Ri,Kim Eun-Young,Kim Si Hyun,Lee Hae Kyung,Lee Jaehyeon,Shin Jong Hee,Kim Young Ree,Song Sae Am,Jeong Joseph,Uh Young,Kim Yu Kyung,Yong Dongeun,Kim Hyun Soo,Kim Sunjoo,Kim Young Ah,Shin Kyeong 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.1
Background: Streptococcus pneumoniae is a serious pathogen causing various infections in humans. We evaluated the serotype distribution and antimicrobial resistance of S. pneumoniae causing invasive pneumococcal disease (IPD) after introduction of pneumococcal conjugate vaccine (PCV)13 in Korea and investigated the epidemiological characteristics of multidrug-resistant (MDR) isolates. Methods: S. pneumoniae isolates causing IPD were collected from 16 hospitals in Korea between 2017 and 2019. Serotyping was performed using modified sequential multiplex PCR and the Quellung reaction. Antimicrobial susceptibility tests were performed using the broth microdilution method. Multilocus sequence typing was performed on MDR isolates for epidemiological investigations. Results: Among the 411 S. pneumoniae isolates analyzed, the most prevalent serotype was 3 (12.2%), followed by 10A (9.5%), 34 (7.3%), 19A (6.8%), 23A (6.3%), 22F (6.1%), 35B (5.8%), 11A (5.1%), and others (40.9%). The coverage rates of PCV7, PCV10, PCV13, and pneumococcal polysaccharide vaccine (PPSV)23 were 7.8%, 7.8%, 28.7%, and 59.4%, respectively. Resistance rates to penicillin, ceftriaxone, erythromycin, and levofloxacin were 13.1%, 9.2%, 80.3%, and 4.1%, respectively. MDR isolates accounted for 23.4% of all isolates. Serotypes 23A, 11A, 19A, and 15B accounted for the highest proportions of total isolates at 18.8%, 16.7%, 14.6%, and 8.3%, respectively. Sequence type (ST)166 (43.8%) and ST320 (12.5%) were common among MDR isolates. Conclusions: Non-PCV13 serotypes are increasing among invasive S. pneumoniae strains causing IPD. Differences in antimicrobial resistance were found according to the specific serotype. Continuous monitoring of serotypes and antimicrobial resistance is necessary for the appropriate management of S. pneumoniae infections.
꽈배기 모자반 물 추출물의 항산화능과 물리적 특성에 대한 감마선 조사의 영향
김아람(Ah-Ram Kim),송유진(Eu-Jin Song),김미정(Mi-Jung Kim),이소영(So-Young Lee),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김진희(Jin-Hee Kim),김서진(Seo-Jin Kim),홍용기(Yong-Ki Hong),박진규(Jin-Gyu Park),김재훈(Jae-Hun Kim),이주운(Ju-Woon Lee) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.3
본 연구에서는 꽈배기모자반 물 추출물에 식품산업에서 사용이 확대되고 있는 감마선 조사기술을 이용하여 항산화능의 변화를 알아보고, 꽈배기모자반 물 추출물의 높은 점성과 짙은 색상으로 인한 문제점을 개선하고자 하였다. 꽈배기모자반 물 추출물의 감마선 조사에 따른 항산화능의 변화를 알아보기 위해 총 페놀화합물 함량을 측정하고 DPPH radical 소거능을 측정하였다. 그 결과, 3~20 kGy의 감마선을 조사한 꽈배기모자반 물 추출물은 조사선량이 증가할수록 총 페놀화합물 함량이 증가하였고, DPPH radical 소거능도 증가하여 감마선 조사에 의해 항산화능이 증진되었다. 그리고 꽈배기모자반 물 추출물에 감마선 조사 시 물리적 특성 변화를 알아보기 위해 색상과 점도를 측정한 결과, 감마선 조사선량이 증가할수록 색이 옅어지고 점성이 감소하는 것으로 나타났다. 이를 통해 꽈배기모자반 물 추출물에 감마선을 조사한 후 식품에 적용 시 가공특성을 개선시킬 수 있을 것으로 사료된다. 따라서 감마선 조사는 꽈배기모자반 물 추출물의 물리적 특성을 개선시키고 항산화능 또한 증진시키므로 식품산업에 적용 시 기존의 천연 항산화제의 단점을 개선시킬 수 있는 기술로서 사용이 확대될 수 있을 것으로 사료된다. In this study, the antioxidant properties and physical characteristics of irradiated Sargassum siliquastrum water extract were evaluated. Samples were irradiated with Co<SUP>60</SUP> γ-ray at doses ranging from 3 to 20 kGy. They were then analyzed to investigate antioxidant properties, including total phenolic compound content and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. In addition, physical properties such as viscosity and color were evaluated. The results demonstrated that total phenolic compound content and DPPH radical scavenging activity were significantly improved (p<0.05) by irradiation. In terms of physical properties, viscosity and color were reduced in the irradiated extracts as compared to non-irradiated samples. In conclusion, gamma irradiation improved the antioxidant properties of Sargassum siliquastrum water extract, improving its original weak point as a natural antioxidant when applied in the food industry.
Kim Si Hyun,Sung Gyung-Hye,Park Eun Hee,Hwang In Yeong,Kim Gyu Ri,송새암,Lee Hae Kyung,Uh Young,Kim Young Ah,Jeong Seok Hoon,Shin Jong Hee,Shin Kyeong Seob,Lee Jaehyeon,Jeong Joseph,Kim Young Ree,Yong Do 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.2
Salmonella is one of the major causes of food-borne infections. We investigated the serotype distribution and antimicrobial resistance of Salmonella isolates collected in Korea between January 2016 and December 2017. In total, 669 Salmonella isolates were collected from clinical specimens at 19 university hospitals. Serotyping was performed according to the Kauffmann–White scheme, and antimicrobial susceptibility was tested using Sensititre EUVSEC plates or disk diffusion. Among the strains, C (39.8%) and B (36.6%) were the most prevalent serogroups. In total, 51 serotypes were identified, and common serotypes were S. enterica serovar I 4,[5],12:i:- (16.7%), S. Enteritidis (16.1%), S. Bareilly (14.6%), S. Typhimurium (9.9%), and S. Infantis (6.9%). The resistance rates to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole were 32.6%, 12.1%, and 8.4%, respectively. The resistance rates to cefotaxime and ciprofloxacin were 8.1% and 3.0%, respectively, while 5.4% were multidrug-resistant. S. enterica serovar I 4,[5],12:i:- and S. Enteritidis were highly prevalent, and there was an increase in rare serotypes. Multidrug resistance and ciprofloxacin resistance were highly prevalent. Periodic investigations of Salmonella serotypes and antimicrobial resistance are needed.
Changes in Allergenicity of Porcine Serum Albumin by Gamma Irradiation
Kim, Koth-Bong-Woo-Ri,Lee, So-Young,Song, Eu-Jin,Park, Jin-Gyu,Lee, Ju-Woon,Byun, Myung-Woo,Kim, Kyu-Earn,Ahn, Dong-Hyun Korean Society for Food Science of Animal Resource 2010 한국축산식품학회지 Vol.30 No.3
Pork is an excellent source of essential nutrients such as protein. However, pork can trigger hypersensitivity and serum albumin of pork is known as major allergen. In this study, to evaluate the effect of gamma irradiation on the allergenicity of porcine serum albumin (PSA), PSA solution was irradiated at 3, 5, 7, 10, 15, and 20 kGy. The changes in the ability of PSA to bind IgG and patient's serum caused by gamma irradiation were observed by ci-ELISA and immunoblotting. SDS-PAGE was used for measuring the conformational change of gamma-irradiated PSA. The ability of 3-kGy-irradiated PSA to bind p-IgG and patient's serum was decreased to 30% and 15%, respectively. The binding ability showed no significant differences among all irradiated samples. SDS-PAGE showed that the irradiated PSA bands were degraded and aggregated. Immunoblotting of irradiated PSA revealed that IgG and patient's serum were rarely recognized at 3 kGy. Therefore, gamma irradiation could be applied to less-allergenic pork products.
Construct Validation of Occupational Stress Scale in Korean Dental Technician
Gyu Ri Kim,Chung Jae Lee 대한예방치과학회 2014 International Journal of Clinical Preventive Denti Vol.10 No.4
Objective: The purpose of this study was to find out the stress level of dental technologist by using Korean Occupational Stress Scale (KOSS), to check for its appropriate for dental technologist and search for the method to measure in-depth stress factors. Methods: The study targets were 116 dental technologists; for the measurement of occupational stress level, 24 shortened form questions of KOSS developed by, were used. The stress level was grasped depending on the general characteristics by using T test or ANOVA of SPSS ver. 18.0, and stress factors of dental technologists were analyzed through structural matrix and factor analysis using Oblimin containing Kaiser normalization. Results: It was found that 6 factors were more appropriate than 7 sub factors that were proposed when KOSS was developed. It was also found that there was a relationship between working hours and stress, and the items with high stress showed high job autonomy (time pressure, excessive work load, irregular rest and work characteristics requiring several works at the same time. Conclusion: It was evaluated that KOSS was appropriate for the measurement of dental technologist's stress. It is necessary to search for the method to analyze the stress factors that are more appropriate for dental technologist and to solve stress continuously.
Kim, Gyu Ri,Jee, Sun Ha,Pikhart, Hynek BMJ Publishing Group Ltd 2018 Journal of epidemiology & community health Vol.72 No.6
<P>Conclusions Our findings provide support for the mediating role of AL and health behaviours in the link between SEP and mortality. Policies designed to reduce social disparities in mortality in the long term should primarily focus on reducing stress and promoting healthy lifestyles among the socially disadvantaged groups. Future studies should further assess the role of other mediators such as psychosocial factors, which may contribute to socioeconomic inequalities in mortality.</P>
Gyu-Ri Kim(김규리) 한국산학기술학회 2015 한국산학기술학회논문지 Vol.16 No.7
이 연구는 아크릴 수지의 유도광선변화에 따른 속성 변화를 연구하여 최적의 특성을 가진 아크릴 수지를 개발할 수 있도록 건열 및 마이크로파 경화 의치상 아크릴 수지의 향상된 물성에 대한 연구이다. 굽힘강도와 경도, 색차를 관찰한 결과 Vertex RS 및 Paladent 20는 건식 열 경화 시편 5, 15, 25 kGy의 조사에 이상적인 속성을 보였다. 마이크로 웨이브 경화 에서는 5 kGy의 조사에서 이상적인 속성을 보였다. 상관관계 분석은 ARD와 굴곡 강도 (0 418), E 계수 (0.675), 및 바콜 경도 (0 588) 사이의 긍정적인 상관관계를 보여 주었다. 방사선 경화형 기술은 고분자 복합 재료의 제작으로 인한 오염 문제 를 완화하는 데 도움이 될 수 있으며 고분자의 가공성에 영향을 주지 않고 자외선 경화 기술과 나노 기술 융합 및 기계적 특성의 향상에 크게 기여할 수 있다. The purpose of this study was to research the property change of acrylic resins depending on the induced-beam change and research the improved physical property of dry-heat and microwave-cured dental place acrylic resin in order to develop the acrylic resins with the optimum characteristic. As a result of observing flexural rigidity, hardness and color difference, the dry-heat-cured specimens of Vertex RS and Paladent 20 showed ideal property at 5, 15, and 25 kGy irradiation. The microwave-cured specimens of Vertex RS and Paladent 20 showed ideal property at 5 kGy irradiation. The correlation analysis showed a positive correlation among ARD, flexural rigidity (0 418), E coefficient (0.675) and Barcol hardness (0 588). The radiation cure technology is helpful for relieving the contamination caused by the manufacture of polymer composite. It can significantly contribute to the fusion of ultra violet cure technology and nano technology and the improvement of mechanical property without giving effect to the workability of polymer.