http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Park, Sujin,Yang, Kyung-Min,Park, Yuna,Hong, Eunji,Hong, Chang Pyo,Park, Jinah,Pang, Kyoungwha,Lee, Jihee,Park, Bora,Lee, Siyoung,An, Haein,Kwak, Mi-Kyung,Kim, Junil,Kang, Jin Muk,Kim, Pyunggang,Xiao, Korean Society of Cancer Prevention 2018 Journal of cancer prevention Vol.23 No.1
<P><B>Background</B></P><P>Smad3 linker phosphorylation plays essential roles in tumor progression and metastasis. We have previously reported that the mutation of Smad3 linker phosphorylation sites (Smad3-Erk/Pro-directed kinase site mutant constructs [EPSM]) markedly reduced the tumor progression while increasing the lung metastasis in breast cancer.</P><P><B>Methods</B></P><P>We performed high-throughput RNA-Sequencing of the human prostate cancer cell lines infected with adenoviral Smad3-EPSM to identify the genes regulated by Smad3-EPSM.</P><P><B>Results</B></P><P>In this study, we identified genes which are differentially regulated in the presence of Smad3-EPSM. We first confirmed that Smad3-EPSM strongly enhanced a capability of cell motility and invasiveness as well as the expression of epithelial-mesenchymal transition marker genes, <I>CDH2</I>, <I>SNAI1</I>, and <I>ZEB1</I> in response to TGF-β1 in human pancreatic and prostate cancer cell lines. We identified <I>GADD45B</I>, <I>CTGF</I>, and <I>JUNB</I> genes in the expression profiles associated with cell motility and invasiveness induced by the Smad3-EPSM.</P><P><B>Conclusions</B></P><P>These results suggested that inhibition of Smad3 linker phosphorylation may enhance cell motility and invasiveness by inducing expression of <I>GADD45B</I>, <I>CTGF</I>, and <I>JUNB</I> genes in various cancers.</P>
Microbial Transformation of Trichostatin A to 2,3-Dihydrotrichostatin A
Park, Je Won,Park, Sung Ryeol,Han, Ah Reum,Ban, Yeon-Hee,Yoo, Young Ji,Kim, Eun Ji,Kim, Eunji,Yoon, Yeo Joon American Chemical Society and American Society of 2011 Journal of natural products Vol.74 No.5
<P>A new reduced hydroxamate, 2,3-dihydrotrichostatin A, was created from trichostatin A by employing a recombinant strain of <I>Streptomyces venezuelae</I> as a microbial catalyst. Compared with trichostatin A, 2,3-dihydrotrichostatin A showed similar antifungal activity against <I>Saccharomyces cerevisiae</I>, but, interestingly, approximately twice the cytostatic activity against human small-cell lung cancer cells. The production of 2,3-dihydrotrichostatin A via microbial biotransformation demonstrates that the regiospecific and substrate-flexible hydrogenation by <I>S. venezuelae</I> provides a new approach for creating natural product analogues with improved bioactive properties.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2011/jnprdf.2011.74.issue-5/np1006718/production/images/medium/np-2010-006718_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np1006718'>ACS Electronic Supporting Info</A></P>
Park, Jae-Hee,Shin, Eunji,Park, Eunju The Korean Society of Food Science and Nutrition 2017 Preventive Nutrition and Food Science Vol.22 No.3
Aged garlic has been reported to possess beneficial pharmacological activities, including anti-stress and anti-fatigue properties, and to exert protective effects on the cardiovascular system and liver. Pine needles are widely used in folk medicine and as food additives owing to their pharmacological properties such as anti-aging and anti-inflammatory effects. It has long been known that combining certain phytochemicals with other phenols or organic acids can produce synergistic effects. Therefore, the purpose of this study was to develop an optimal formula of aged garlic with added pine needle powder for improved antioxidant activity using the statistical technique of response surface methodology. The antioxidant activities of aged garlic mixed with pine needle powder were confirmed by measuring oxygen radical absorbance capacity and total polyphenol content. An optimized antioxidant formula was identified that contained 5.08 g aged garlic and 1.97 g pine needle powder. The antioxidant activities of the mixture prepared using this optimal formula were significantly higher than the predicted values according to an additive model. Hence, this study confirms that the addition of pine needle powder to aged garlic can improve its antioxidant activity. This study demonstrated an optimal mixing ratio to produce an aged garlic product with improved functionality through the addition of pine needle powder that could be successfully employed by the food industry to prepare functional foods.
Anti-inflammatory effect of torilidis fructus ethanol extract through inhibition of Src
Park, Gyubyung,Kim, Eunji,Son, Young-Jin,Yoon, Deok Hyo,Sung, Gi-Ho,Aravinthan, Adithan,Park, Yung Chul,Kim, Jong-Hoon,Cho, Jae Youl SWETS AND ZEITLINGER 2017 PHARMACEUTICAL BIOLOGY Vol.55 No.1
<P><B>Abstract</B></P><P><B>Context:</B> Torilidis fructus, fruits of <I>Torilis japonica</I> Decadolle (Umbelliferae), is a medicinal herb traditionally used as a pesticide, an astrictive, or a medicine for various inflammatory diseases.</P><P><B>Objectives:</B> Due to the lack of pharmacological studies on this herbal medicine, we explored the inhibitory activity of torilidis fructus on the macrophage-mediated inflammatory response using its ethanol extract (Tf-EE).</P><P><B>Material and methods:</B> The Griess assay and prostaglandin (PGE<SUB>2</SUB>) ELISA assay were conducted with Tf-EE (0-75 µg/mL) and LPS (1 µg/mL) treated RAW264.7 cells in cultured media. Tf-EE pretreated RAW264.7 cells were incubated with LPS for 6 h and semi-quantitative PCR was performed. Reporter gene assays, overexpression of target enzymes and immunoblotting were performed on macrophages to determine the molecular targets of Tf-EE.</P><P><B>Results:</B> Tf-EE markedly suppressed the inflammatory response of macrophages, such as lipopolysaccharide (LPS)-induced nitric oxide (NO) and PGE<SUB>2</SUB> production with IC<SUB>50</SUB> values of 35.66 and 62.47 µg/mL, respectively. It was also found that Tf-EE reduced the expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 by 80%. Nuclear translocation and activation of nuclear factor (NF)-κB (p65 and p50) were declined by 60% and 30% respectively, and their regulatory events including the phosphorylation of AKT, IκBα, Src, and the formation of complexes between Src and p-p85 were also recognized to be diminished.</P><P><B>Conclusions:</B> The signalling events managed by Src and p85 complex seemed to be critically involved in Tf-EE-mediated anti-inflammatory response. This might suggest that Tf-EE exhibited anti-inflammatory effects through Src-targeted inhibition of NF-κB.</P>