세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

확대된 여가제약 척도의 최적범주

이창진(Lee, Chang-Jin),김응준(Kim, Eung-Joon) 한국웰니스학회 2017 한국웰니스학회지 Vol.12 No.1

본 연구는 김응준(2012)이 Rasch 모형을 적용하여 선행연구에서 개발된 확대된 여가 제약 척도의 심리측정학적 특성 결과를 바탕으로 최적범주를 탐색하는데 목적이 있었다. 이 목적을 달성하기 위해 대학교 410명의 학생들을 대상으로 조사를 실시하고 통계분석하였다. 사용된 척도는 Crawford와 Godbey(1987)에 의해 제시된 여가제약 척도를 지현진과 이철원(2003)이 수정 보완하고, 김응준(2006)에 의하여 타당화 시킨 확대된 여가 제약 척도를 조사도구로 사용하였다. 수거된 자료 중 410명의 자료를 SPSS 23.0프로그램을 이용하여 빈도분석, 기술통계분석을 실시하였다. 최적범주를 탐색하고자 WINSTEPS 3.92 (Linacre, 2016)을 사용하여, 척도수의 적절성, 문항적합도와 문항난이도 등을 산출하였다. 연구목적에 따른 연구결론으로는 첫째, 문항의 반응범주는 문제가 있는 것으로 확인되어 기존 5점 범주 대신 4점 범주로 수정되는 것이 타당하였다. 둘째, 문항적합도를 분석한 결과 22문항은 적합하였다. 셋째, 척도의 응답자 신뢰도가 낮게 확인되어 문항난이도를 고려한 문항순서의 변경이 필요하였다. 넷째, 개인 능력점수와 난이도를 교차하여 비교한 결과, 개인 능력점수 분포와 문항난이도 분포는 적절하였다. The purpose of this study was to investigate the extended leisure constraint questionnaire’s optimal categorization in undergraduate students. To accomplish study purpose, complete enumeration survey and statistical analysis were conducted for 410 students enrolled university. A research tool used in this study was an extended leisure constraint scale developed by Eung-Joon KIM (2012), which was originally from Crawford & Godbey(1987). Total 22 questions were used with 5 point Likert scale and high point stands for higher the leisure constraint. Frequency analysis was conducted with SPSS 23. An assumption to test Rasch Model, which is satisfaction of unidimensionality, is regarded through theoretical aspects, and WINSTEPS 3.92(Linacre, 2016) program is used to test the goodness-of-fit of items. Statistical analysis showed results as follows. First, 4-point rating scale was appropriate for the extended leisure constraint scale. Second, 22 items of the extended leisure constraint scale were suitable in item fitness and item difficulty to undergraduate students. Third, the extended leisure constraint scale was somewhat suitable in person reliability of item separation. Fourth, the person ability score of the extended leisure constraint scale was distributed appropriately.

직장암 수술 후 국소재발과 예후

김태윤,백무준,김성용,신응진,박내경,이문수,김창호,송옥평 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Locally recurrent rectal cancer is a difficult clinical problem. Local recurrence following treatment for rectal cancer is a major cause of morbidity and mortality. The reported range of local recurrence rates following surgical treatment for rectal cancer has varied from 4% to 50%. We retrospectively reviewed the charts of 142 rectal cancer patients from January 1991 to December 1999. Analysis of patients factors included age, gender, type of surgery, and adjuvant therapy. Tumors were assessed for level, stage, and grade. Local recurrence and distant metastases were also documented. Variables influencing local recurrence in this group were identified and disease-free survival is determined. Of the 142 patients(74 males, 68 females; mean age 62.6 years), 137 consecutive patients operated on for rectal cancer and followed up to December 2000 (range 23-118months) were analyzed. Local recurrence rate was 18.3%. Age and sex of patients, type of surgery, location of tumor in the rectum, size, morphology and grading of the tumor were all unrelated to the event under investigation. At Cox regression, the Dukes stage and the postoperative radiotherapy were the only independent prognostic factors for local recurrence, and Dukes stage (Hazard ratio=2.89, p<0.001) and local recurrence (Hazard ratio=3.31, p<0.01) were the only independent factors associated with improved survival.

데빅병 치료에 사용한 Interferon β-1b의 부작용 1예

김정미,조은경,조정선,최용석,한영수,한정호,김두응,최진영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

A 63 year-old woman who had diagnosis of multiple sclerosis complaining left hemiparesis and fluctuating decreased visual acuity in her right eye and abnormal findings in pattern shift visual evoked potentials(PSVEPs) and on T2 weighted MR scanning. We treated her subcutaneously with 250μg interferon (3-lb(ITFß -lb) every other day. There were detected pancytopenia, skin rash, and abnormal liver function test seven days after the therapy. In spite of reducing the dose of interferon injection, abnormal laboratory findings were not returned to normal. Dose adjustment of interferon-I3 for body weight or race, may be helpful to reduce the side effects.

Landsat TM Data를 이용한 해안 환경림 조사

박용진,김응남 강릉대학교 동해안지역연구소 2000 국제 심포지움 Vol.2000 No.1

Osteogenic Sarcoma에 관한 연구

김영일,김영진,박응천,최원재 大韓口腔顎顔面 放射線學會 1993 Imaging Science in Dentistry Vol.23 No.1

The author observed a case of osteogenic sarcoma in a 11-year-old female with complaint of painful swelling on face in fight side. The observed results were as follows: 1. Large hematoma was observed, and patient complanited painful swelling on c/c site. 2. Predisposing factor of osteogenic sarcoma was not clear, but patient had history of extraction before patient visiting infirmary of our dental collage. 3. Serologic findings were not specific, and serum alkaline level was normal. 4. Radiographic findings were as follows: ① Diffuse faint radiopacity in the lesion ② Bony destruction and increased radiopaciy in right antrum ③ Displacement of multiple teeth on involved area(i. e #12, 15, 55, 16, 17, 18) ④ Increased periodontal space in single tooth(#13) ⑤ Destruction of bony crypt on involved teeth(#13, 14, 15, 17, 18) ⑥ Loss of lamina dura of three teeth in involved area(#11, 12, 16) 5. Computed tomographic findings were as follows: ① Large calcific and hetergenous component mass in the Rt. maxillary sinus, and this mass extending to Rt. maxilla, alveolar bone, ethmoid sinus. ②Soft tissue bulging into Rt. side nasal cavity and oral cavity. ③ Bone destruction of maxillary sinus wall and Rt. alveloar bone.

대동맥판막에 발생한 유두상 섬유탄력종 1례

김응중,최재성,이명묵,김어진 동국대학교 의학연구소 2009 東國醫學 Vol.16 No.1

유두상 섬유탄력종은 주로 심장판막에 발생하는 원발성 종양으로 심장종양 중 두 번째로 많은 양성종양이나 전체적으로 그 빈도는 매우 드물다. 78세 여자 환자가 흉부 불편감을 주소로 내원해서 대동맥판막에 위치한 유두상 섬유탄력종이 발견되어 개심술 하에 대동맥판막의 손상 없이 종양을 제거하였기에 문헌고찰과 더불어 이를 보고하는 바이다. Cardiac papillary fibroelastoma is the rare primary cardiac tumor, though it is the second most common benign cardiac tumor. It usually involves the cardiac valve. Papillary fibroelastoma of the aortic valve was found in a 78-year-old woman with chest discomfort and it was excised without valvular injury under cardiopulmonary bypass. Thus we report the present case with relevant review of the literatures.

Sports Massage에 따른 혈액성분 변화에 관한 연구

김관진,유응식 공주영상정보대학 1999 논문집 Vol.6 No.-

인천소재 재학중인 고등학교 태권도 선수 10명을 대상으로 Massage요법 시술 전후의 안정시와, 운동후의 RBC, WBC, HCT, Hb, ALB, T-Chol의 변화에 대해 얻어진 결론은 다음과 같다. 1) 적혈구(RBD)는 Massage 시술전의 안정시의 평균치는 487(만)에서 운동후에는 528(만)으로 0.84% 증가치클 보였으며, 시술후의 안정시의 평균치는 500(만)으로 나타났으며, 운동후에는 563(만)으로 0.8% 증가치를 보였다. 시술전후의 안정시의 경우 시술전보다 시술후가 0.96%증가치를 나타냈으며, 운동후의 경우에는 시술전보다 시술 후가 0.91% 증가치를 보였다. 2) 백혈구(WBC)는 Massage시술전의 안정시의 평균치는 7,619㎎/dl로 나타났으며, 운동후에는 8,574㎎/dl로서 0.89% 증가치를 보였으며, 시술후의 안정시의 평균치는 8,718㎎/dl로 나타났으며, 운동후에는 9,717㎎/dl로서 0.9% 증가치를 보였다. 시술전후의 안정시의 경우 시술전보다 시술후가 0.87% 증가치를 나타냈으며, 운동후의 경우, 시술전보다 시술후가 0.92% 증가치를 보였다. 3) Hematocrit(Hct) Hematocrit는 Massage 시술전의 안정시의 평균치는 44.11g/dl로 나타났으며, 운동후는 47.1g/dl로 안정시보다 0,93증가치를 보였으며, 시술후의 안정시 평균치는 46㎎/dl, 운동후는 51.7g/dl 증가하였으며 안정시보다 0.89%의 증가치를 나타냈다. 시술전후의 안정시의 경우 시술전보다 시술 후가 0.96%의 증가치를 보였으며, 운동후의 경우 시술전보다 시술 후가 0.91% 증가치를 보였다. 4) Hemoglobin(Hb)은 Massage 시술전의 안정시의 평균치는 14.4㎎/dl에서 운동후에는 15㎎/dl로서 0.94% 증가치를 보였으며, 시술후의 안정시의 평균치는 15.4㎎/dl, 운동후는 16.5㎎/dl로서 0.93% 증가치를 보였다. 시술전후의 안정시의 경우, 시술전보다 시술후가 0.94%로 증가치를 보였다. 시술전후의 안정시의 경우 시술전보다 시술후가 0.94%로 증가치를 나타냈으며, 운동후의 경우, 시술전보다 시술후가 0.92%증가치를 보였다. 5) Albumin(ALB)은 Massage 시술전의 안정시 평균치는 4.5g/dl, 운동후는 4.8g/dl로 0.93% 증가치를 보였으며, 시술후의 안정시의 평균치는 4.7g/dl, 운동후의 평균치는 5.1g/dl로서 0.92% 증가치를 보였다. 시술전후의 안정시의 경우, 시술전보다 시술후가 0.96% 증가치를 보였으며, 운동후의 경우, 시술전보다 시술후가 0.94% 증가치를 보였다. 6) Cholesterol은 Massage 시술전의 안정시 평균치는 129mmg/이, 운동후의 평균치는 140mg/dl로 0,92% 증가치를 나타내었으며, 시술후의 경우에는 안정시의 평균치가 141mg/dl, 운동후의 평균치가 152mg/dl로서 0.9% 증가치를 보여주었다. 시술전후의 안정시의 경우, 시술전보다 시술후가 0.91% 증가치를 나타내었으며, 운동후의 경우 시술전보다 시술후가 0.92% 증가치를 보였다.

오존層保護를 위한 分爭解決節次

김효진,엄응용 경운대학교 산업기술연구소 1999 産業技術硏究論文誌 Vol.1 No.1(B)

This Article explores state responsibility to the international community as a whole and injured states in particular for the damage occurring from the production and use of ozone depleting substances. This Article further explores the remedies available to the international community and to individual states injured by ozone layer depletion. The Article describes international dispute resolution techniques that states may employ in the face of continued polluting activities by other states.

비장애 아동과 전반적 발달장애 아동에서 회전 후 안구진탕 기간의 비교

김진미,송지원,홍은경,김성희,김경미 대한감각통합치료학회 2005 대한감각통합치료학회지 Vol.3 No.1

Objective : The purpose of this study was to get the mean of duration of post-rotatory nystagmus test in normal children and to differentiate the duration of post-rotatory nystagmus test between normal children and children with pervasive developmental disorder. Method : 84 subjects were between 3 and 5 years of age and consisted of 64 normal children and 20 children with the pervasive developmental disorder. Analysis of the data was done by using t-test and ANOVA. Results : The results were as follows: 1. Range of duration of post-rotatory nyatagmus test in normal children was 5-22second on left and 7-21 second on right and the mean was 12.63 second on left and 12.59 second on right. 2. Range of duration of post-rotatory nystagmus test in children with the pervasive developmental disorder was 3-11 second on both and the mean was 5.65 second on left and 5.60 second on right. 3. There was no significant difference between males and females with normal children in duration of post-rotatory nystagmus test. However, there was a significant difference of the mean duration between 3 and 5 years old normal group. 4. Children with pervasive developmental disorder significantly have relatively lower duration than the duration of post-rotatory nystagmus test of normal children. Conclusions : The results of the study showed significant difference between normal children and children with pervasive developmental disorder in duration of post-rotatory nystagmus test and suggest that they could be applied to the baseline of clinical therapy.