구상나무에 있어서 Inter - Simple Sequence Repeats Marker 의 유전양식

홍용표,조경진,김용율,신은명 ( Yong Pyo Hong,Kyung Jin Cho,Yong Yul Kim,Eun Kyeong Shin ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.3

Polymerase chain reaction(PGR)-based inter-simple sequence repeats(I-SSR) markers were analyzed in 48 megagametophytes of a single tree of Abies koreana W_(ILS). Nineteen of the 35 primers, screened with 6 megagametophyte DNA and produced the clearest amplification products in the preliminary experiment, were used for PCR with 48 megagametophyte DNAs sampled from a single tree. On the basis of the chi-square test, a total of 51 amplicons, amplified by the 19 primers, were revealed to be segregated according to the Mendelian ratio(i.e., 1 : 1 segregation ratio) in the 48 megagametophytes at 5% significance level. Based on the linkage analysis, the observed 51 Mendelian loci turned out to be unlinked each other, which suggested that they are evenly distributed in the genome. However, majority of RAPD markers are known to belong to the independent linkage blocks, which frequently results in the amplification of RAPD markers from the restricted regions of the genome. Owing to the nature of even distribution of the 51 loci observed in this study, the I-SSR markers could give better resolution of estimating genetic diversity from the whole genome than RAPD markers. And I-SSR markers are also more suitable than RAPD markers for reconstructing phylogenetic relationship by a cladistic method which requires to fulfil the assumption of independent evolution of the different characters.

염기조건에서 Dynemicin A에 관련된 모델 화합물들의 에폭시드 열림에 대한 치환체 효과

홍용표,김정희,Hong, Yong Pyo,Kim, Jung Hee 대한화학회 2000 대한화학회지 Vol.44 No.5

음식물류폐기물 공공 자원화시설 운영에 관한 연구 (Ⅰ)

홍용표ㆍ김혜선ㆍ김웅용ㆍ신현곤(Yong Pyo HongㆍHye Sun KimㆍUng Yong KimㆍHyun Gon Shin) 유기성자원학회 2016 유기물자원화 Vol.24 No.1

본 연구는 음식물류 폐기물 공공 자원화 시설 운영의 문제점과 제도 개선방안을 위하여 실시하였다. 기존 음식물류 폐기물 공공자원화 시설에 대한 실태조사를 통해 운영에 따른 문제를 분석하였으며, 이를 위하여 음식물류 폐기물의 발생량 및 자원화 현황, 공공 및 민간 자원화시설 현황, 전국의 주요 음식물류 폐기물 공공자원화 시설의 실제 운영과정에서 나타난 문제점을 분석한 결과는 다음과 같다: 현재 국내 음식물류 폐기물의 분리 수거량은 약 97%인 것으로 나타났다. 그리고 대부분의 공공 자원화시설은 음식물류 폐기물의 자원화가 아닌 단순처리에 급급한 것으로 나타났으며 경제성도 없는 것으로 분석되었다. 특히, 바이오가스시설 대부분은 실시설계와는 달리 가스 생산량 및 수요량이 전혀 맞지 않는 것으로 나타났다. This study is conducted to find out problems of the public food waste recycling facility and its improvement. Through a research on the actual condition, it is possible to analyze the problem of operation. Moreover, for this improvement, with analysis of the current state of recycling rate including its generation and the problem that can be shown from the real operation of the public/private food waste recycling facility, the results are as follows: It can be shown that the current amount of domestic food waste resource recycling is about 97 %. Almost every public recycling facility is analyzed to be economically infeasible and is not for recovery but to simple disposal. Especially, most of Biogas facilities appeared that amount of production and demand is not appropriate differed from enforcement design.

음식물류폐기물 공공 자원화시설 운영에 관한 연구 (I) - 운영현황과 문제점을 중심으로 -

홍용표,김혜선,김웅용,신현곤,Hong, Yong-Pyo,Kim, Hye-Sun,Kim, Ung-Yong,Shin, Hyun-Gon 유기성자원학회 2016 유기물자원화 Vol.24 No.1

This study is conducted to find out problems of the public food waste recycling facility and its improvement. Through a research on the actual condition, it is possible to analyze the problem of operation. Moreover, for this improvement, with analysis of the current state of recycling rate including its generation and the problem that can be shown from the real operation of the public/private food waste recycling facility, the results are as follows: It can be shown that the current amount of domestic food waste resource recycling is about 97 %. Almost every public recycling facility is analyzed to be economically infeasible and is not for recovery but to simple disposal. Especially, most of Biogas facilities appeared that amount of production and demand is not appropriate differed from enforcement design. 본 연구는 음식물류 폐기물 공공 자원화 시설 운영의 문제점과 제도 개선방안을 위하여 실시하였다. 기존 음식물류 폐기물 공공자원화 시설에 대한 실태조사를 통해 운영에 따른 문제를 분석하였으며, 이를 위하여 음식물류 폐기물의 발생량 및 자원화 현황, 공공 및 민간 자원화시설 현황, 전국의 주요 음식물류 폐기물 공공 자원화 시설의 실제 운영과정에서 나타난 문제점을 분석한 결과는 다음과 같다: 현재 국내 음식물류 폐기물의 분리 수거량은 약 97%인 것으로 나타났다. 그리고 대부분의 공공 자원화시설은 음식물류 폐기물의 자원화가 아닌 단순처리에 급급한 것으로 나타났으며 경제성도 없는 것으로 분석되었다. 특히, 바이오가스시설 대부분은 실시설계와는 달리 가스 생산량 및 수요량이 전혀 맞지 않는 것으로 나타났다.

소나무와 곰솔간 이입교잡종으로 추정되어온 금강송에 있어서 곰솔 cpDNA 의 부재

홍용표,김규식,노의래,신은명,김진수 ( Yong Pyo Hong,Kyu Sik Kim,Eui Rae Noh,Eun Myeong Shin,Zin Suh Kim ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.4

Portions of chloroplast genes(psbD and rbcL) were amplified from Pinus thunbergii(Japanese black pine : black pine) and Pinus densiflora(Japanese red pine : red pine) by PCR and digested by a restriction enzyme, HaeIII, respectively. Two species specific cpDNA markers were identified. With the observed cpDNA markers, paternal inheritance of cpDNA in pine hybrids was verified in an artificial hybrid family between black pine(Chollanam 37) and red pine(Chungchongbuk 3). On the basis of paternal inheritance of chloroplast genome in a hybrid. 2 portions of cpDNA amplified from 115 individuals of Pinus densiflora for. erecta were screened to detect any traces of black pine specific cpDNA markers in P densiflora for. erecta which has been postulated as an introgressive hybrid between red pine and black pine(Hyun el al., 1967). All the analyzed individuals of Pinus densiflora for, erects revealed the identical profiles of HaeIII digested psbD and rbcL genes to red pine. This result suggests that there is no introduced chloroplast genome of black pine in Pinus densiflora for. erecta and that there is no concrete evidence of treating P. densiflora for, erecta as an introgressive hybrid between red pine(♀) and black pine(♂).

음식물류폐기물 공공 자원화시설 운영에 관한 연구 (Ⅱ)

홍용표ㆍ김웅용ㆍ신현곤(Yong Pyo HongㆍUng Yong KimㆍHyun Gon Shin) 유기성자원학회 2016 유기물자원화 Vol.24 No.1

본 연구는 음식물류폐기물 공공 자원화 시설 운영의 문제점과 제도 개선방안을 위하여 실시하였다. 현재 운영중인 음식물류 폐기물의 가동율 현황과 자원화 시설의 확충 계획에 대한 분석을 통해 시설의 무분별한 신규건설로 인한 국가의 예산이 낭비되지 않도록 그 대안을 제시하였으며, 그 결과는 다음과 같다. 공공 자원화시설 신규 확충보다는 기존 민간 자원화시설을 활용하여 예산을 절약하고 음식물류 폐기물의 안정적인 처리는 물론 자원순환을 촉진시킬 수 있는 정책전환이 필요하다. 음식물류 폐기물을 친환경적인 방법으로 안정적으로 처리하기 위해서는 제도개선이 필요하며, 이를 위해 민간 자원화시설 가동률 저하를 줄일 수 있는 준공영제 도입이 필요하다. 또한, 공공 자원화시설의 검증된 기술과 경제성 확보 방안 마련이 필요하다. This study is conducted to find out the problems of the public food waste recycling facility operation and its improvement. In order to save the national budget by analyzing plans to expand including operation rate for the food waste recycling facility, the alternative ways are suggested. The result can be as follows: Policy switch is required not only to save budget and stable disposal of food waste but also to promote resource recycling rather than expanding public facilities. In order to change the policy for stable and eco-friendly disposal of food waste, it is necessary to adopt semipublic system that prevent the decline of the rate of operation of private resource recycling facility. Furthermore, it is required to prepare both qualified technologies of public resource recycling and the ways of profitability secure.

백두산 반달가슴곰의 분자유전학적 특성 분석

홍용표,김원명,신은명,양병국 ( Yong Pyo Hong,Won Myong Kim,Eun Myeong Shin,Byung Guk Yang ) 한국산림과학회 1998 한국임학회 학술발표논문집 Vol.1998 No.-

No Trace of Introduced cpDNA of Pinus thunbergii in Pinus densiflor for. erecta Postulated as an Introgressive Hybrid between Pinus densiflora and Pinus Thunbergii

홍용표,김규식,노의래,신은명,김진수,Hong, Yong-Pyo,Kim, Kyu-Sik,Noh, Eui-Rae,Shin, Eun-Myeong,Kim, Zin-Suh Korean Society of Forest Science 1998 한국산림과학회지 Vol.87 No.4

Portions of chloroplast genes(psbD and rbcL) were amplified from Pinus thunbergii(Japanese black pine : black pine) and Pinus densiflora(Japanese red pine : red pine) by PCR and digested by a restriction enzyme, HaeIII, respectively. Two species specific cpDNA markers were identified. With the observed cpDNA markers, paternal inheritance of cpDNA in pine hybrids was verified in an artificial hybrid family between black pine(Chollanam 37) and red pine(Chungchongbuk 3). On the basis of paternal inheritance of chloroplast genome in a hybrid, 2 portions of cpDNA amplified from 115 individuals of Pinus densiflora for. erecta were screened to detect any traces of black pine specific cpDNA markers in P. densiflora for. erecta which has been postulated as an introgressive hybrid between red pine and black pine(Hyun el al., 1967). All the analyzed individuals of Pinus densiflora for. erects revealed the identical profiles of HaeIII digested psbD and rbcL genes to red pine. This result suggests that there is no introduced chloroplast genome of black pine in Pinus densiflora for. erecta and that there is no concrete evidence of treating P. densiflora for, erecta as an introgressive hybrid between red pine(♀) and black pine(♂). 소나무와 해송으로부터 엽록체상의 두 유전자 psbD와 rbcL를 PCR에 의해 증폭한 후 제한효소 HaeIII를 사용해서 절단했다. 두 개의 종 특이적 엽록체 DNA 단편이 확인되었고, 이 두 개의 표지자를 이용하여 소나무(충북3호)와 해송(남난37호)의 인공교잡 가계로부터 엽록체 DNA의 부계 유전양식이 확인되었다. 인공교잡 가계에 있어서 엽록체 DNA의 부계 유전양식을 근거로 해송으로부터 소나무로의 이입교잡에 의해 생겨났다는 가설(현신규 등, 1967)이 지배적인 금강송 115개체로부터 이입교잡에 의해 유입되어진 흔적을 구명하기 위하여 해송 특이 엽록체 DNA의 존재 여부를 검색하였다. 분석에 사용된 금강송 전 개체에서 소나무에서 관찰된 엽록체 DNA(psbD와 rbeL)의 절편 분획 양상과 동일한 절편 분획 양상이 확인되었다. 본 실험의 결과로부터는, 금강송에는 해송으로부터 유입된 엽록체 게놈의 흔적을 찾아 볼 수 없었으며, 따라서 금강송을 소나무(♀)약 해송(♂)의 이입교잡종이이라고 간주할만한 확고한 증거를 제시할 수 없었다.

상류 낙동 강변의 광미 내 중금속 오염현황

홍용표 ( Yong Pyo Hong ),최종하 ( Jong-ha Choi ),김연정 ( Younjung Kim ),김대익 ( Dae-ik Kim ),유건상 ( Keon Sang Ryoo ) 한국환경분석학회 2016 환경분석과 독성보건 Vol.19 No.3

Ore powder had been cumulated at a moderate incline along the upper Nakdong Riverside by the natural disaster such as a flood from mines and smelting factories between 1940 and 1970. This study investigates the surface and deep layer mine tailings and the neighboring soil at 7 sites in the upper Nakdong River System. The study analyzed the sites between 2014 and 2015 for the presence of eight heavy metals (Cd, Pb, As, Cr(VI), Hg, Zn, Cu, Mn), Generally, the content of heavy metals increased in order from the contrast soil, the deep layer mine tailings, to the surface layer mine tailings. Consequently, it was concluded that the upper region of the Nakdong Riverside requires the remediation in the near future in order to remove the mine tailings.

국내 자생 철쭉나무 집단에 있어서의 I-SSR 변이체 분포양상

홍용표 ( Hong Yong Pyo ),권해연 ( Kwon Hae Yeon ),김용률 ( Kim Yong Lyul ),김찬수 ( Kim Chan Su ),한상돈 ( Han Sang Don ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.5

Distribution pattern and level of genetic diversity in 6 populations of an endemic shrub in Korea, smile rosebay (Rhododendron schlippenbachii), were monitored by analyzing a molecular genetic marker, inter-simple sequence repeats (I-SSR). From the results of PCR with 5 I-SSR primers, a total of 61 I-SSR, variants were observed in 120 smile rosebay trees. All the analyzed individuats could be distinguished by pooling all the observed I-SSR markers. Moderate level of genetic diversity within populations was observed, ranged from 0.360 (Gwangneung) to 0.456 (Mt. Gaya) with the mean of 0.395. From the results of AMOVA at 2 hierarchical levels, 88.38% of I-SSR variants was allocated among individuals within populations, which resulted in moderately high degree of population differentiation in this species (Φ_(ST)=0.116). From the results of AMOVA at 3 hierarchical levels, moderate degree of genetic differentiation was observed among geographic regions (Φ_(CT)=0.05O) and among populations within groups (Φ(CT_=0.076). This observation suggested that genetic differentiation among geographic groups might be partially responsible for the observed moderately high degree of population differentiation at 2 hierarchical levels (Φ_(ST)=0.116). Reconstruction of genetic relationships among 6 population by UPGMA, revealed 2 geographic groups (Mts. Dykyu, Gaya, and Sobaek; Mt. Seorak and Gwangneune) and a most distantly related population of Mt. Taebaek to both groups. Overall patterns of genetic relationships among populations were relatively well coincided with geographic affinity among populations. Observed results in this study could provide molecular genetic information on future supplementing of any shortage in genetic diversity in the natural populatins of smile rosebay.