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사람 파필로마 바이러스 18형 외피 단백질 L1의 대장균에서의 발현
진승원,권두환,손우익,김현수,최인성,박순희 대한바이러스학회 1994 Journal of Bacteriology and Virology Vol.24 No.2
Human papillomavirus(HPV) type 18 capsid protein Ll was expressed in E. coli BL-21 strain under the control of T7 promotor by cloning the Ll open reading frame(1704 nucleotides). The gene encoding HPV 18 Ll protein was amplified by PCR and cloned into pET-3a E. coli expression vector. Recombinant 18L1 protein expressed in BL-21 strain was confirmed by 12% SDS- PAGE followed by Western blotting with rnonoclonal antibody against HPV 16L1. We also cloned Ll derivative encoding C-terminus deleted Ll protein into pET-3a vector. C-erminus deleted protein was detected by HPV 16L1 monoclonal antibody.
진승원,박철휘,박종현,김선명,신미정,정명선,김형욱,박선숙,안유배,장윤식,방병기 대한내과학회 1997 대한내과학회지 Vol.53 No.3S
저자들은 이전에 저칼륨혈증을 치료받았던 환자에서 갑자기 발생한 전신적인 근 무력감을 토대로 검사한 결과 H^+ -ATPase 분비 장애(secretory defect)에 의한 원위성 세뇨관 산증에 의한 신장을 통한 과량의 칼륨 손실로 인해 생긴 저칼륨 혈증에 의한 주기성마비 1예를 경험하였다. 원위성 세뇨관 산중에 의한 저칼륨 주기성 마비는 국내에 보고된 바가 없기에 문헌 고찰과 더불어 처음으로 보고 하는 바이다. Peridoic muscle paralysis is characterized by acute reversible generalized muscular weakness and absent deep tendon reflexes, usually sparing sensation and consciousness. Although hypokalemia is common in renal tubular acidosis(RTA), acute hypokalemic periodic paralysis(HPP) is extremely rare and not known in Korea. A 27-year--old man admitted due to generalized muscular weakness. We observed a hypokalemia (initial serum postassium=2.2mEg/L), hyperchloremic metabolic acidosis (initial serum pH=7.324 and serum chloride= 111mEg/L), and relative alkaline urine (initial urine pH=6.611) and diagnosed with hypokalemic periodic paralysis by distal renal tubular acidosis suspiciously. In this patient an ammonium chloride (NH4CL, 0.lg/㎏) loading test failed to decrease urine pH below 5.5 when blood pH decreased to 7.229. The furosemide test was done to evaluated a underlying mechanism of distal RTA which the fractional excretion of potassium incrased from 33.5% to 57.5% but urine pH did not significantly changed from 6.30 to 6.21. These results suggested that his distal RTA was probably due to H^+-ATPase secretory defect. In this case, we confirmed hypokalemic periodic paralysis in a young man complicated with distal RTA due to H^+-ATPase pump defect and managed successfully by potassiun and bicarbonate replacement.
Preparation of Polyimide Powder via Hydrothermal Polymerization for Compression Molding Application
진승원,최윤제,김담비,윤강훈,정찬문 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Polyimides (PIs) have been receiving attention as high-performance materials because of their outstanding thermal and mechanical performance, and chemical resistance based on rigid imide structure. In addition, PI powder can be used in manufacturing lightweight molding parts where precision, thermal, and dimensional stability are critical such as bearing and sealing parts. However, general synthesis methods of PIs are employing high-boiling and toxic solvents such as dimethylformamide (DMF), N,N-dimethylacetamide (DMAc), and N-methyl-2-pyrrolidone (NMP), which are hazardous to the environment. Moreover, these organic solvents lead to high production costs. In this study, we developed a green synthesis method to synthesize PI powder via hydrothermal polymerization. In addition, thermal and mechanical properties of compression-molded specimens prepared with PI powder were investigated for their application to compression molding.