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포도구균의 Erythromycin 유도성 Clindamycin 내성검출을 위한 간편한 디스크 확산법의 유용성
주세익 ( Sae Ick Joo ),이현 ( Hyun Lee ),임규상 ( Kyu Sang Lim ),김의종 ( Eui Chong Kim ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.1
A simple and easy modification of AST by disk diffusion was tested for the detection of induced clindamycin resistant Staphylococci and their antimicrobial susceptibility at the same time. The incidence of inducible clindamycin resistant staphylococci in blood culture and their MIC characterization at Seoul National University Hospital was analyzed by an AST contained disk approximation test (D-zone test) and Etest, respectively. Of the total 309 staphylococcal isolates, 139 (45%) isolates presented constitutive resistance to ERY and CLI (ERY-R, CLI-R phenotype), and 59 were ERY-I/R and CLI-S phenotypes. Of the 59 isolates, 19 (32%) isolates were inducible resistant to CLI. The incidence was higher in S. aureus (66.7%) than coagulase-negative staphylococci (CNS, 26.0%). Especially, methicillin-resistant staphylococci (MRSA, 100%; MRCNS, 45.5%) presented higher inducibility than methicillin susceptible (MSSA, 50%; MSCNS, 20%). For most of the inducible clindamycin resistant staphylococci (15 of 19 isolates), their ERY MIC were high (>128 μg/mL) and were methicillin resistant. The remaining 4 isolates were methicillin susceptible and their ERY MIC were of intermediate concentrations (1-4 μg/mL). We concluded that suscetibility testing of staphylococci, especially methicillin resistant, should include the D-zone test.
Isolation of Rotaviral RNA from Stool Specimens and Determination of It's Electropherotypes
Joo, Se-Ik,Kim, Kyung-Hee,Cho, Young-Guel,Barnett, Bill B.,Yang, Jai-Myung 成均館大學校 科學技術硏究所 1990 論文集 Vol.41 No.1
환자의 설사변으로부터 사람 로타바이러스를 간단하게 정제하는 방법을 기술하였다. 정제된 로타바이러스로부터 추출한 genomic RNA들은 polyacrylamide gel 전기영동으로 분리된 후 질산은 염색법과 3', 5'-[5'^32P]pCp를 이용한 3'-말단표지법으로 확인하였다. 모든 설사변은 group 특이적 항원(VP6)을 이용한 ELISA로 검사되었으며, 총 143개 양성 설사변 중 111개에서 로타바이러스의 전기영동형을 관찰하기에 충분한 양의 RNA가 추출되었고, 모든 음성 설사변과 비설사변에서는 관찰되지 않았다. 이 연구에서 '짧은 전기영동형'(subgroup I)과 '긴 전기영동형'(subgroup II), 그리고 '혼합형'이 관찰되었으며 긴 전기영동형 중 두 개의 아형(Long-1 과 Long-2)도 관찰되었다.
홍기호,주세익,김의종,신수,노은연,윤종현 대한임상미생물학회 2012 Annals of clinical microbiology Vol.15 No.1
Background: Diagnosis of nontuberculous mycobacterium (NTM) is challenging, and clinical, radiological and microbiological criteria should be met. Traditionally,culture results on solid media have been reported semi-quantitatively, but no study exists regarding the clinical significance of low-colony count culture reports. The authors of the present study analyzed the clinical significance of low-colony count specimens of NTM with a greater than three-year follow-up period. Methods: A total of 341 clinical isolates were evaluated among the isolates at Seoul National University Hospital and Seoul National University Borame Hospital from October 2005 to September 2006. Colony count less than 50 was considered a low-colony count specimen. Identifications of NTM from all the isolates were performed using a DNA chip (PCR reverse hybridization,LG Life Science, Korea). Clinical significance was analyzed by reviewing the medical records of patients with greater than three years of follow-up data after NTM isolation from respiratory samples. Results: NTM lung disease was observed in 27.0% of the patients with low-colony count specimens among 167 patients with respiratory samples, and 70.4% of the patients were treated. The low-colony count patients had less NTM lung disease, longer incubation period, and less acid fast bacilli-positivity than patients with a colony count greater than 50. Conclusion: The prevalence of NTM lung disease with a low-colony count specimen was greater than 25%. In a clinical setting, NTM lung disease should not be excluded only on the basis of a low-colony count.
김선영,주세익,이종윤,김의종 대한진단검사의학회 2010 Annals of Laboratory Medicine Vol.30 No.2
Although the association between Streptococcus bovis endocarditis and colon carcinoma is well known, very few cases of S. bovis infection associated with underlying malignancies have been reported in Korea. The S. bovis group has been recently reclassified and renamed as Streptococcus gallolyticus and Streptococcus infantarius subspecies under a new nomenclature system. We report a case of infective endocarditis with colon cancer caused by S. gallolyticus subsp. gallolyticus (previously named S. bovis biotype I). A 59-yr-old woman presented with a 1-month history of fever. Initial blood cultures were positive for gram-positive cocci, and echocardiography showed vegetation on mitral and aortic valves. Antibiotic treatment for infective endoc arditis was started. The infecting strain was a catalase-negative and bile-esculin-positive alpha-hemolytic Streptococcus susceptible to penicillin and vancomycin. The strain was identified as S. gallolyticus subsp. gallolyticus with the use of the Vitek 2 GPI and API 20 Strep systems (bioMérieux, USA). The 16S rDNA sequences of the blood culture isolates showed 100% homology with those of S. gallolyticus subsp. gallolyticus reported in GenBank. The identification of the infecting organism, and the subsequent communication among clinical microbiologists and physicians about the changed nomenclature, led to the detection of colon cancer. The patient recovered after treatment with antibiotics,valve surgery, and operation for colon cancer. This is the first report of biochemical and genetic identification of S. gallolyticus subsp. gallolyticus causing infective endocarditis associated with underlying colon cancer in a Korean patient. (Korean J Lab Med 2010;30:160-5)
A Case of Melioidosis Presenting as Migrating Pulmonary Infiltration: The First Case in Korea
김영환,주세익,강영애,윤영순,유철규,한성구,심영수,김의종,이종윤,임재준,이세원 대한의학회 2005 Journal of Korean medical science Vol.20 No.1
Melioidosis is an infection of the Gram-negative bacterium Burkholderia pseudoma-llei. While it is known as an important cause of sepsis or chronic abscess-formingdisease in Southeast Asia and northern Australia, no case has yet been reported inKorea. A 50-yr-old man visited our hospital for intermittent fever associated with drycough and sputum. Roentgenographic examination showed migrating pulmonaryinfiltration. Symptoms and chest radiograph and computed tomography (CT) imagefindings did not improve despite use of fluoroquinolone antibiotics. Gram-negativebacteria were isolated on bronchoscopic washing culture and were identified as B. pseudomallei on DNA sequencing of 16S ribosomal RNA with 100% homology. Treatment for melioidosis was commenced with high dose ceftazidime, and the pa-tient’s fever, cough, and sputum were improved and the lesion on chest radiograph and CT almost disappeared.
김의종,주세익,석종성 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1
Virkon(Antec International) is a balanced, stabilized blend of peroxygen compounds, surfactants, organic acids and an inorganic buffer system. It contains a triple salt; potassium monopersulphate, potassium hydrogen sulphate, and potassium sulphate. In order to evaluate the disinfection activities of Virkon against microrganisms, macrodilution broth tube method was performed for bacteria and yeast, and dye uptake assay using 96-well microtiter plate for virus. 0.25% Virkon killed all of 15 strains of Staphylococcus aureus and 10 strains of Pseudomonas aeruginosa within 30 seconds in the presence of 1% plasma. 0.5% concentration killed all of 15 strains of Escherichia coli within 30 seconds in the presence of 1% plasma. All of ATCC strains of Bacillus spp. were killed with 3% Virkon, but two of three strains of Bacillus thuringiensis were not killed with 3% Virkon after 16 minutes. Five of 10 strains were not killed with 3% Virkon after 16 minutes. Five of 10 strains of Candida albicans were not killed iwth 1% Virkon after one minute. Infectivities of Herpes simplex virus were disapperaed at 0.1% concentration within one minute. The present study showed that Virkon was effective for the disinfection of bacteria and virus, but less effective against spore-forming gram-positive bacteria and yeast, and the appropriate concentration of Virkon was one percent for the general purpose.