토양선충분리(土壤線蟲分離)를 위(爲)한 려과지(濾過紙) 선발(選拔)에 관(關)하여

조명래,최영연 한국식물병리학회 1985 한국식물보호학회지 Vol.24 No.1

모빌리티와 사회 이후 사회

조명래 한국공간환경학회 2011 한국공간환경학회 학술대회 논문집 Vol.2011 No.3

감자 주산지 식물기생선충 분포와 뿌리혹선층의 PCR 동정

조명래,이영규,김점순,유동림,Cho Myoung-Rae,Lee Young-Gyu,Kim Jum-Soon,Yoo Dong-Lim 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.1

국내 주요감자재배지의 식물기생선충 발생상을 밝히기 위해 2004년과 2005년에 제주, 예산, 김천, 고령, 홍천, 평창, 김제, 밀양, 남원, 강릉, 인제 등 11개 감자 주산지를 대상으로 선충속별 종류와 밀도를 조사하였다. 채집한 토양시료 50개 중 30개에서 뿌리혹선충 유충이 검출되었으며 밀도는 12-69마리/토양 100cc 정도로 비교적 낮았다. 식물기생선충류 중 Pratylenchus sp., Helicotylenchus sp, Ditylenchus sp., Tylenchus sp., and Tylenchorhynchus sp.가 지역별로 검출되었으나 대부분 밀도는 매우 낮게 나타났다. 채집한 토양을 토마토 뿌리에 접종 후 뿌리혹선충을 배양하여 PCR-RLFP에 의한 정밀동정 결과 제주, 밀양, 고령에서 채집한 뿌리혹선충 암컷에서 500 bp의 PCR 산물을 얻었으며 제한 효소 Dra I을 처리한 결과 290 및 230 bp의 밴드가 형성되어 당근뿌리혹선충(Meloidogyne hapla)으로 동정하였다. This study was conducted to get basic information on the occurrence of plant-parasitic nematodes for the establishment of nematode management strategy in major potato production areas in Korea. Nationwide soil collection was done in 11 areas of Cheju, Yesan, Gimchun, Goryoung, Hong chun, Pyungchang, Gimjae, Milyang, Namwon, Gangnung, and Inje in 2004-2005. Root-hot nematode juveniles(J2) were detected in 30 samples among the 50 samples. The average density was 12-69 J2/100cc soil. Pratylenchus sp., Helicotylenchus sp., Ditylenchus sp., Tylenchus sp., and Tylenchorhynchus sp. were also detected in various locations, however, their densities were very low. Root-knot nematode females were collected from tomato roots inoculated with the potato field soils for PCR-RFLP identification. The females from Cheju, Milyang, and Goryung showed PCR products of 500 bp. And the Dra I restriction enzyme digestions showing 290 bp and 230 bp fragments confirmed their identity as Meloidogyne hapla.

심비디움에 발생하는 해충의 종류

조명래,전성욱,강택준,김형환,안승준,양창열,Cho, Myoung Rae,Jeon, Sung-Wook,Kang, Taek Joon,Kim, Hyung Hwan,Ahn, Seung-Joon,Yang, Chang Yeol 한국응용곤충학회 2013 한국응용곤충학회지 Vol.52 No.4

수출용 심비디움에 발생하는 해충의 종류와 해충관리 현황을 알아보고자 국내 주요 재배단지 10개 지역 45농가를 대상으로 조사를 실시하였다. 농가에서 채집된 해충의 종류는 응애류(20농가), 깍지벌레류(6농가), 민달팽이류(6농가), 총채벌레류(8농가), 진딧물류(5농가) 노린재류(1농가) 등이었다. 채집한 해충은 현미경을 이용하여 동정한 결과 점박이응애(Tetranychus urticae Koch), 대만총채벌레(Frankliniella intonsa Trybom), 난초핀깍지벌레(Pinnaspis aspidistrae Signoret), 민달팽이(Incilaria confusa Cockarel), 썩덩나무노린재(Halyomorpha brevis Walker), 복숭아혹진딧물(Myzus persicae Sulzer), 목화진딧물(Aphis gossypii Glover), 무화과깍지벌레(Coccus hesperidum Linnaeus)와 소형달팽이류 1종으로 나타났다. 총채벌레 산란피해로 의심되는 증상의 정확한 동정을 위해 rDNA의 ITS2 부위를 PCR-RFLP 한 결과 아까시총채벌레(Thrips flavus Schrank)와 파총채벌레(Thrips tabaci Lindeman)로 확인되었다. 따라서, 수출 심비디움을 재배하는 농가에서는 이러한 해충들에 대한 종합적인 방제대책을 수립하여 실천하는 것이 필요할 것으로 생각된다. A survey of pest occurrence and status of farmer's pest management was conducted at 45 cymbidium farms in 10 major cultivation areas in Korea. The pest species collected from the cymbidium farms were identified as follows: Tetranychus urticae Koch, Frankliniella intonsa Trybom, Pinnaspis aspidistrae Signoret, Incilaria confusa Cockarel, Halyomorpha brevis Walker, Myzus persicae S$\ddot{u}$lzer, and Aphis gossypii Glover, Coccus hesperidum Linnaeus, Thrips flavus Schrank, and Thrips tabaci Lindeman. The two-spotted spider mite, T. urticae, was the key pest in cymbidium production, occurring on 45 farms, followed by scales (20 farms), slugs (6), thrips (8), aphids (5), and stinkbug (1). PCR-RFLP of the rDNA ITS2 region revealed that two thrips species, Thrips flavus Schrank and Thrips tabaci Lindeman, occur on cymbidium farms. Therefore, it is necessary for the cymbidium farmers to establish an integrated pest management system to meet quarantine standards.

심비디움에 발생하는 선충 조사 및 줄기선충류(Ditylenchus sp.)에 대한 약제방제 효과

조명래,강택준,김형환,안승준,전성욱,전재용,김영호,Cho, Myoung-Rae,Kang, Taek-Joon,Kim, Hyung-Hwan,Ahn, Seung-Joon,Jeon, Sung-Uk,Chun, Jae-Yong,Kim, Young-Ho 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.2

수출 심비디움 재배 시 검역상 문제되는 선충의 발생 여부를 알아보고자 전국의 심비디움 재배단지에서 수집한 1-3년생 심비디움 근권부에 발생하는 선충의 종류를 조사하였다. 1-3년생 심비디움 근권부에서 관찰된 식물기생성 선충은 Ditylenchus속으로 밀도는 배지 200 cc 당 각각 4.7, 43.7, 49.7마리였다. 자유생활성 선충류인 Dorylaimid 선충의 밀도는 배지 200 cc당 각각 35.3, 70.5, 155.8마리였다. 시흥, 안산, 김포의 3년생 심비디움에서는 Aphelenchus속, Aphelenchoides속 선충이 관찰되었으나 화분 당 40마리 이하로 밀도가 낮았으며 Dorylaimid가 화분 당 56-824마리로 나타났다. 선충방제용 약제선발을 위해 Emamectin benzoate EC, Fosthiazate SL, Cadusafos CS 등 3종의 약제로 안산과 김포의 2개 농가에서 방제효과를 시험하였다. Emamectin benzoate EC는 2개소 시험에서 75.7, 89.5%의 방제가를 보였으며 Fosthiazate SL, Cadusafos CS는 각각 27.2, 65.3% 및 30.1, 90.5%의 방제가를 나타내었다. Surveys were conducted on the occurrence of nematodes in the root systems of 1-3-year old Cymbidium hybida Swartz cultivated for export in Korea. The most frequently detected plant-parasitic nematode was Ditylenchus sp. with 4.7, 43.7, and 49.7/200 cc growth medium in 1, 2, and 3 year-old cymbidiums, respectively. And the densities of non-parasitic nematodes, dorylaimids, were 35.3, 70.5, and 155.8/200 cc growth medium in 1, 2, and 3-year-old cymbidiums, respectively. Three-year-old cymbidiums collected from Siheung, Ansan, and Gimpo had low densities of Aphelenchus sp. and Aphelenchoides sp. with under 40 individuals/pot, and the dorylaimid densities were 56-824/pot. To evaluate the effects of nematicides on Ditylenchus sp. in cymbidium, Emamectin benzoate EC, Fosthiazate SL, and Cadusafos CS were tested at two farms in Ansan and Gimpo. Emamectin benzoate EC showed control effects of 75.7 and 89.5%, whereas Fosthiazate SL and Cadusafos CS showed 27.2 and 65.3% and 30.1 and 90.5% control effects in the tests.

레이저 용접을 위한 박판재의 정밀 전단 메카니즘 개발 : 유한요소법을 이용한 정밀 전단 공정해석 Analysis of Precise Shearing Process using FEM

조명래,표창률,전병희 한국산업안전학회 1999 한국안전학회지 Vol.14 No.4

Recently, Tailor Welded Blank (TWB) is widely used in automotive industry since the trans formation characteristic of its material can be changed. However, clearance between welding surfaces becomes the important factor which affect the quality of the laser weld, causing difficulties in preparing the sheet. The objective of this paper is to systematically evaluate the effects of previously presented fracture criterion and shearing condition on precise mechanical shearing simulation result. For this purpose, a parametric study was performed to investigate the effect of finite element size and fracture criterion on simulation result. Also, in order to predict the optimum shearing condition, effect of shearing conditions such as clearance and punch radius on the shear plane shape was evaluated.

The Effect of Positive Medial Cortical Support in Reduction of Pertrochanteric Fractures with Posteromedial Wall Defect Using a Dynamic Hip Screw

조명래,이재혁,권재범,도정석,Seung Bum Chae,최원기 대한정형외과학회 2018 Clinics in Orthopedic Surgery Vol.10 No.3

Background: We evaluated the radiological and clinical results of reduction using a dynamic hip screw according to the grade of medial cortical support in patients with AO Foundation and Orthopaedic Trauma Association (AO/OTA) classification type 31-A2 pertrochanteric fractures. Methods: We enrolled 100 patients with AO/OTA type 31-A2 fractures with displaced lesser trochanter fragments (length of the cortical area longer than 20 mm on the pelvis anteroposterior view). Patients with positive medial cortical support were assigned to group 1 (n = 28); neutral medial cortical support, group 2 (n = 42); and negative medial cortical support, group 3 (n = 30). Radiological evaluation was done by measuring the change in the femoral neck-shaft angle and sliding distance of the lag screw. Clinical outcomes of each group were compared by means of the walking ability score proposed by Ceder. Results: Group 1 showed significantly less changes in the femoral neck-shaft angle and shorter sliding distance than groups 2 and 3. Group 2 showed significantly less changes in the femoral neck-shaft angle and shorter sliding distance than group 3. Group 1 showed significantly higher walking ability scores than group 3 (p = 0.00). The use of trochanter stabilizing plates or fixation using wires for posteromedial wall defect resulted in no significant changes in terms of the femoral neck-shaft angle or sliding distance. Conclusions: In the treatment of pertrochanteric fractures accompanied by posteromedial wall defect using a dynamic hip screw, reduction with negative cortical support should be avoided.

국내 산림식물 11종의 항산화 활성

조명래,이종석,이사라,손연경,배창환,여주홍,이해석,마진경,이옥환,김종예,Cho, MyoungLae,Lee, Jong Seok,Lee, Sarah,Son, Youn Kyoung,Bae, Chang-Hwan,Yeo, Joohong,Lee, Hae-Sock,Ma, Jin-Gyeong,Lee, Ok-Hwan,Kim, Jong-Yae 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.6

This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

Comparison of Inoculation Methods, Meloidogyne spp. and Different Host Plants for Production of Pasteuria penetrans

조명래,D. W. Dickson,T. E. Hewlett 한국응용곤충학회 2005 Journal of Asia-Pacific Entomology Vol.8 No.3

Pasteuria penetrans is an effective biolo-gical control agent of root-knot nematode, but its practical use is limited by the lack of efficient me-thods for mass production of the endospores. Root- gal formation by Meloidogyne arenaria and/or M. javanica P. penetrans endospores was compared in plants receiving single vs. split inoculations and in diferent susceptible host plants. Number of endospores produced by M. arenaria was compared among different host plants. Of six host plants tested, M. javanica produced the greatest number of gals on cucumber and M. are-naria produced the greatest number of galls on okra and tomato (P< 0.05). The number of root galls pro-duced by M. javanica was 6-fold greater on cu-cumber inoculated two times at 3-day intervals than with a single inoculation. M. arenaria produced hig-than in the other host plants.

시설작물의 주요해충 방제 대책

조명래 한국시설원예연구회 2003 施設園藝硏究 Vol.16 No.2