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제희정(Hee-Jeong Je),안재욱(Jae-Wook Ahn),윤혜숙(Hae-Suk Yoon),김민근(Min-Keun Kim),류재산(Jae-San Ryu),홍광표(Kwang-Pyo Hong),이상대(Sang-Dae Lee),박영훈(Young-Hoon Park) 한국원예학회 2015 원예과학기술지 Vol.33 No.5
Powdery mildew (PM) caused by Podosphaera aphanis is a major disease that can result in significant yield losses in strawberry (Fragaria × ananassa Duchesne). For preventing PM, pesticides are usually applied in strawberry. In this study, molecular markers were developed to increase breeding efficiency of PM-resistance cultivars by marker-assisted selection (MAS). An F₂ population derived from a cross between PM-resistance ‘Seolhyang’ and PM-susceptibility ‘Akihime’ was evaluated for disease resistance to PM and RAPD (random amplification of polymorphic DNA)-BSA (bulked segregant analysis). Among 200 RAPD primers tested, OPE10 primer amplified a 311bp-band present in with 331bp. Sequence alignment performed for searching polymorphisms and six single nucleotide polymorphism (SNP) were found in amplified regions. To develop polymorphic marker for distinguishing between resistant and susceptible, RAPD was converted to cleaved amplified polymorphic sequence (CAPS) marker. Among restriction enzymes associated with six SNPs, Eae I (Y/GGCCR) was successfully digested to 231bp in susceptible. The results suggest that the selected CAPS marker could be used for increasing efficiency of selecting powdery mildew resistant strawberry in breeding system.
Hee-Jeong Je(제희정),Yeo-Ok Park(박여옥),Sung-Churl Kim(김성철),Ji-Hyun Hwang(황지현),Yong-Jae Lee(이용재),Beung-Gu Son(손병구),Young-Hoon Park(박영훈) 한국원예학회 2009 원예과학기술지 Vol.27 No.3
The genetic relationships among 60 oriental persimmon (Diospyros kaki Thunb.) accessions including 30 pollination-constant non-astringent (PCNA), 16 pollination-variant non-astringent (PVNA), 6 pollination-variant astringent (PVA), and 8 pollination-constant astringent (PCA) cultivars were evaluated using 39 RAPD (randomly amplified polymorphic DNA)-PCR primers. A total of 185 polymorphic bands out of 250 RAPD bands scored were obtained and unique fingerprints for all 60 cultivars were produced, despite inclusion of closely related bud-sport cultivars. Pair-wise genetic similarity coefficient (Nei-Li) among all pairs of 60 cultivars varied from 0.62 (between ‘Taishu’ and ‘Saijo’) to 0.99 (between ‘Superhiratanenashi’ and ‘O-tanenashi’). Unweighted pair-group method with arithmetic averaging (UPGMA) clustering analysis revealed two main clusters, Ⅰ and Ⅱ; all 30 PCNA cultivars formed cluster Ⅰ and showed a narrow genetic diversity among themselves (0.85-0.99). Cluster Ⅱ contained PVNA cultivars and other astringent type cultivars. Principle component analysis (PCA) showed a third group consisting of seven PVA cultivars, in addition to cluster Ⅰ and Ⅱ that were revealed by UPGMA clustering. RAPD-based phenetic relationships among the persimmon cultivar were comparable to known pedigree records, morphological observations, and reports from previous DNA fingerprinting studies that used different molecular marker types. Our study demonstrated that RAPD markers can be efficiently used for genetic diversity assessment of closely related persimmon varieties and cultivar identification, which are essential for modern breeding program.
큰느타리(Pleurotus eryngii) 품종 판별을 위한 초위성체 유래 다중 표지 개발
임착한 ( Chak Han Im ),김경희 ( Kyung Hee Kim ),제희정 ( Hee Jeong Je ),알리아스자드 ( Asjad Ali ),김민근 ( Min Keun Kim ),정완규 ( Wan Kyu Joung ),이상대 ( Sang Dae Lee ),신현열 ( Hyunyeol Shin ),류재산 ( Jae San Ryu ) 한국균학회 2014 Mycobiology Vol.42 No.2
For development of a method for differentiation of Pleurotus eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotyping results. PCR using each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in the range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pairgroup method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into two clusters. Cluster I and II were comprised of four and eight cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivars and strains with 21 alleles and a PIC value of 0.9090. These results might be useful in providing an efficient method for the identification of P. eryngii cultivars with separate PCR reactions.