Zoledronate이 UMR-106 세포의 증식과 조골세포 형성에 미치는 영향

정기훈,류동목,지유진,이덕원,이현우,Jeong, Ki-Hoon,Ryu, Dong-Mok,Jee, Yu-Jin,Lee, Deok-Won,Lee, Hyun-Woo 대한치과의사협회 2008 대한치과의사협회지 Vol.46 No.10

Purpose : The purpose of this study is to identify the effect of zoledronate(Zometa(R)), which is most common nitrogen containing bisphosphonate, on survival, proliferation, and differentiation of osteoblast. Material & Methods: Twenty four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x $10^4 cells per plates. Each plates were incubated with 5% $CO^2 incubator at $37^{\circ}C$. Starting from 2 days after incubation, cell culture medias were replaced, and added with osteogenesis induction media and 0, 0.01, 0.1, 0.5, 1, $3\muM$ of zoledronate(Zometa(R)), every 2 days, for 12 days. Control group was plates not added with zoledronate($0\muM$), and experiment group were plates added with different concentration of zoledronates(0, 0.01, 0.1, 0.5, 1, $3\muM$). Mature osteoblasts were identified with Alizarine Red staining, and protein samples were collected. Optical density was determined at wavelength of 405nm with ELISA reader. For viability analysis, cells were harvested and incubated with propidium iodide, and analysed with flow cytometry. Western blot technique was used to analyse Runx2 protein of osteoblast. Results : Secretion of bone matrix decreased as zoledronate concentration increased, and zoledronate did not effect survival rate of UMR-106 cells when measured with flow cytometer. Expression of Runx2 protein was inhibited as zoledronate concentration increased. Conclusion : From the results, we were able to identify that increase of zoledronate concentration inhibited differentiation of UMR-106 cell to osteoblast, without effecting quantity or survival rate.

양측 하악과두 골절에 대한 관혈적 정복술후 나타난 근약화와 시력장애 - 증례보고

정기훈(Ki Hoon Jeong),전인성(In Seong Jeon),신용길(Yong Gil Shin),김태열(Tae Youl Kim),신재명(Jae Myung Shin),윤규호(Gyu Ho Yoon) 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.6

The condylar fracture show high occurrence rate in the following of symphysis and angle, because it is easy to have concentration of outer pressure due to its long and narrow anatomical shape and mouth opened state when trauma is attacked. For the clinical findings, there are trismus, opening deviation, malocclusion and open bite. The classification of condylar fracture is varied due to the location of condylar bone fragment, fracture site. There are conservative and surgical methods for treatment modalities. Because of many advantages, conservative method is widely used it has no particular limits of treatment adjustment but the cases of absolutely surgical correction indication are easily found in clinical experience. Although many methods are suggested for treatments of condylar fracture, there are no particular surgical methods for precise reduction due to difficult accessibility in high condylar neck fracture. For precise reduction of condyle, we used Nam's method. However, in Nam's method, because condyle is fixed when once it is kept off externally reduced in avascular state, so complication such as condyle resorption is pointed out as a problem. In our clinic, we obtained some knowledge by treating a patient who had muscle weakness and vision disorder after temporary airway obstruction following the both condylar fracture operated using Nam's method, so here we reported the case with a study of documents.

Zoledronate가 UMR-106 세포의 증식과 조골세포 형성에 미치는 영향

정기훈(Ki-Hoon Jeong),류동목(Dong-Mok Ryu),지유진(Yu-Jin Jee),이덕원(Deok-Won Lee),이현우(Hyun-Woo Lee) 대한치과의사협회 2008 대한치과의사협회지 Vol.46 No.10

Purpose : The purpose of this study is to identify the effect of zoledronate(Zometa<SUP>®</SUP>), which is most common nitrogen containing bisphosphonate, on survival, proliferation, and differentiation of osteoblast. Material & Methods: Twenty four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 × 10⁴ cells per plates. Each plates were incubated with 5% CO² incubator at 37℃. Starting from 2 days after incubation, cell culture medias were replaced, and added with osteogenesis induction media and 0, 0.01, 0.1, 0.5, 1, 3 μM of zoledronate(Zometa<SUP>®</SUP>), every 2 days, for 12 days. “Control group” was plates not added with zoledronate(0μM), and “experiment group” were plates added with different concentration of zoledronates(0, 0.01, 0.1, 0.5, 1, 3μM). Mature osteoblasts were identified with Alizarine Red staining, and protein samples were collected. Optical density was determined at wavelength of 405㎚ with ELISA reader. For viability analysis, cells were harvested and incubated with propidium iodide, and analysed with flow cytometry. Western blot technique was used to analyse Runx2 protein of osteoblast. Results : Secretion of bone matrix decreased as zoledronate concentration increased, and zoledronate did not effect survival rate of UMR-106 cells when measured with flow cytometer. Expression of Runx2 protein was inhibited as zoledronate concentration increased. Conclusion : From the results, we were able to identify that increase of zoledronate concentration inhibited differentiation of UMR-106 cell to osteoblast, without effecting quantity or survival rate.

교통사고 환자 77례에 대한 임상적 고찰

황희상,정기훈,전재천,박재연,김민석,이태호,노정두,이은용,Hwang, Hee-Sang,Park, Jae-Yeon,Kim, Min-Seok,Jeon, Jae-Cheon,Jung, Ki-Hoon,Lee, Tae-Ho,Roh, Jeong-Du,Lee, Eun-Yong 척추신경추나의학회 2009 척추신경추나의학회지 Vol.4 No.2

Objectives : The purpose of this study was to find out tendency of traffic accident patients treated in korean medicine hospital. Methods : We investigated 77 patients who were treated in Semyung University Korean Medicine Hospital from 1 st, January, 2007 to the 30st September, 2009. Results and Conclusions : 1. In distribution of gender and age, the rate of male was 36.4%, that of female was 63.6% and majority of patients were twenties, thirties and forties. 2. In the traffic accident patterns, the most was car crash from behind(49.4%) and after traffic accident, 42 patients(54.6%) visited our hospital in less than 5 days. 3. 22 patients(28.6%) choose the oriental medical treatment for primary care. And 35 patients(45.4%) visited our hospital due to preference of oriental medicine. 4. In partial pain, most was the neck(76.6%), followed by low back(61.0%). 5. In radiation test(excluded sprain and straightening), the most was HIVD of L-SPINE(14.0%). 6. In the periods of admission, less than 7 days was the 1st(36.4%), 14 days was the 2nd(27.3%). 7. The treatment was effective to 67 patients out of 77 patients who had been hospitalized.

평면 이미지 기법을 이용한 분무 특성 해석

이경진,정기훈,윤영빈,정경석,정인석,Lee, Kyung-Jin,Jung, Ki-Hoon,Yoon, Young-Bin,Jeong, Kyung-Seok,Jeung, In-Seuck 대한기계학회 2000 大韓機械學會論文集B Vol.24 No.1

The characteristics of spray nozzle have been quantified with the measurement of fluorescence and Mie scattering images. To correct the attenuation of the incident light sheet, a sequential double-pass light sheet system and the geometrical averaging of two images was implemented. Quantitative mass flux distribution of spray was obtained from fluorescence image. 3-D image is reconstructed using 2-D radial images. Sauter mean diameter (SMD) distribution was determined using the ratio of fluorescence signal intensity and Mie scattering signal intensity and the values were quantified with PDP A data. The measurement of mass flux and SMD using planar imaging technique agee with PDP A data fairly well in the low density region. However, in dense region, there are significant errors caused by secondary scattering. It was found that the planar imaging technique provides many advantages over the point measurement technique, such as PDP A, and can be implemented for quantitative measurement, especially in low density region.

점액표피양 암종의 치험례

황재홍,이승엽,박관수,정기훈,전인성,윤규호,Hwang, Jae-Hong,Lee, Seung-Yeop,Park, Kwan-soo,Jeong, Ki-Hoon,Jeon, In-seong,Yoon, Kyu-Ho 대한악안면성형재건외과학회 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.4

The mucoepidermoid carcinoma is classified as either well, moderately, or poorly differentiated. The criteria used to classify the lesions are discussed, and pathologic features are illustrated. The most important factors in prognosis are : 1. degree of histologic differentiation, and 2. presence or abscence of tumor on the lines of surgical excision. Recurrences rates are correlated with histologic differentiation. Stewart, Foote, and Becker in 1945 coined the term "mucoepidermoid tumor" to discribe an unusual salivary neoplasm containing epidermoid and mucus-secreting cells which was thought to arise in salivsary gland ducts. The treatment of the mucoepidermoid carcinoma is chiefly surgical, although recent data have shown favorable responses to radiation therapy. Currently, surgery followed by radiation treatment is recommended for intermediate-grade and high-grade tumors ; low-grade tumors can be managed by surgery alone. Authors present a case of mucoepidermoid carcinoma managed with wide surgical resection and postoperative irradiation and showing a good clinical result with review of literatures.

요각통 환자에 대한 Bee Venom과 Sweet Bee Venom의 효능 비교 연구

이태호,황희상,장소영,차정호,정기훈,노정두,이은용,Lee, Tae-Ho,Hwang, Hee-Sang,Chang, So-Young,Cha, Jung-Ho,Jung, Ki-Hoon,Roh, Jeong-Du,Lee , Eun-Young 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

Objective The aim of this study is to investigate if Sweet Bee Venom therapy has the equal effect in comparison with Bee Venom Therapy on Low back pain with Radiation pain. Methods Clinical studies were done 24 patients who were treated low back pain with radiation pain to Dept. of Acupuncture & Moxibusition, of Oriental Medicine Se-Myung University from April 1, 2007 to September 30, 2007. Subjects were randomly divided into two groups ; Bee Venom treated group(Group A, n=10), Sweet Bee Venom treatred group(Group B, n=14). In Bee Venom treated group(Group A), we treated patients with dry needle acupuncture and Bee Venom therapy. In Sweet Bee Venom treatred group(Group B), we treated patients with dry needle acupuncture and Sweet Bee Venom therapy. All process of treatment were performed by double blinding method. To estimate the efficacy of controlling pain. we checked Visual Analog Scale(VAS). For evaluating functional change of patients, Straight Leg Raising Test(S.L.R.T) was measured. Results 1. In controlling pain, Sweet Bee Venom treatred group(Group B) had similar ability in comparison with Bee Venom treated group(Group A). 2. In promoting function, Sweet Bee Venom treatred group(Group B) had similar ability in comparison with Bee Venom treated group(Group A). Conclusions It may be equal effects as compared with using Bee Venom to treat low back pain with radiation pain using Sweet Bee Venom. We can try to treat other disease known to have effect with Bee Venom.

전환장애 위증 환자 1례에 대한 증례보고

전재천,황희상,차정호,정기훈,이태호,노정두,이은용,Jeon, Jae-Cheon,Hwang, Hee-Sang,Cha, Jung-Ho,Jung, Ki-Hoon,Lee, Tae-Ho,Roh, Jeong-Du,Lee, Eun-Yong 대한침구의학회 2009 대한침구의학회지 Vol.21 No.4

Objectives : This is a clinical report about the Conversion Disorder, Wei symptom patient. Methods : The patient was treated by acupuncture, herb medication, moxibustion, physical therapy and bio feedback. The improvement of the patient was judged by lower limb elevation test and gait condition test. Results : During the 1st duration, lower limb elevation angle of the patient had improved. And the patient's gait condition had improved. But he received stress so that same symptom appeared again. Then, after the second treatment, He could walk alone again. Conclusions : Oriental medicine treatment including Acupuncture, Herb medication was very effctive to improve the Conversion disorder patient's symptoms. It is necessary to have more observation and many cases of patients with Conversion disorder.

전환장애 위증 환자 1례에 대한 증례보고

전재천,황희상,차정호,정기훈,이태호,노정두,이은용,Jeon, Jae-Cheon,Hwang, Hee-Sang,Cha, Jung-Ho,Jung, Ki-Hoon,Lee, Tae-Ho,Roh, Jeong-Du,Lee, Eun-Yong 대한침구의학회 2009 대한침구의학회지 Vol.26 No.1

Objectives : This is a clinical report about the Conversion Disorder, Wei symptom patient. Methods : The patient was treated by acupuncture, herb medication, moxibustion, physical therapy and bio feedback. The improvement of the patient was judged by lower limb elevation test and gait condition test. Results : During the 1st duration, lower limb elevation angle of the patient had improved. And the patient's gait condition had improved. But he received stress so that same symptom appeared again. Then, after the second treatment, He could walk alone again. Conclusions : Oriental medicine treatment including Acupuncture, Herb medication was very effctive to improve the Conversion disorder patient's symptoms. It is necessary to have more observation and many cases of patients with Conversion disorder.

한국인 총수담관담석의 성분분석

서성옥(Sung Ock Suh),정기훈(Ki Hoon Jeong) 대한외과학회 1991 Annals of Surgical Treatment and Research Vol.41 No.6