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Streptomyces sp. AO-0511이 생산하는 Herbimycin A 및 Dihydroherbimycin A의 이화학적 특성 및 생물 활성
장흥배,김세찬,김재헌,Chang, Hung-Bae,Kim, Se-Chan,Kim, Jae-Heon 한국미생물학회 2006 미생물학회지 Vol.42 No.1
한국 토양에서 방선균주를 분리하고 화학 분류 및 16S rDNA 염기서열을 통하여 Streptomyces 속 균주임을 알아내고 Streptomyces sp. AO-0511로 명명하였다. 이 균주가 생산하는 herbimycin A 및 dihydroherbimycin A의 몇 가지 이화학적 성질과 생물 활성을 측정하였다. 두 물질은 모두 산성 조건에서 안정성을 나타냈으며, dihydroherbimycin A는 herbimycin A에 비해 상대적으로 높은 열 안정성을 지니며 극성 또한 높은 물질로서 TLC 상의 Rf간이 낮았다. Herbimycin A와 dihydroherbimycin A는 모두 Bacillus subtilis ATCC 6633 및 Micrococcus luteus ATCC 9341에 대하여 약한 저해 활성을 나타내었고, 다른 미생물에 대해서는 저해 활성을 나타내지 앓았다. 항암 활성에 있어서 두 물질은 폐암 세포인 AS49세포와 백혈병 세포인 HL-60세포에 대해서 강력한 중식 저해 활성을 나타내었다. L5178Y및 P388세포를 사용하여 세포 독성을 측정하였다. 그 결과 두 물질은 대조 물질인 camptothecin에 비해서 항암 활성을 가지면서도 비교적 안전한 물질임을 알려 주고 있다. A streptomycete strain was isolated from the soil samples from Korea. The chemotaxonomy and 16S rDNA sequencing confirmed that the strain belonged to the genus Streptomyces and we named it Streptomyces sp. AO-0511. Two antibiotics, herbimycin A and dihydroherbimycin A produced by this strain were tested for their physico-chemical and biological characteristics. Both compounds were stable under acidic pH. Dihydroherbimycin A was more heat-stable and polar compared with herbimycin A. Only weak antibacterial activities were detected against Bacillus subtilus ATCC 6633 and Micrococcus luteus ATCC 9341. However, herbimycin A and dihydroherbimycin A showed strong inhibitory activities on lung cancer cells (A549 cells) and leukemia cells (HL-60). The cytotoxicity was determined using L5178Y and P388 cell lines. The results showed that herbimycin A and dihydroherbimycin A had lower toxic effects on the cells compared with the standard compounds, comptothecin and cyclosporin A. Therefore, both compounds could be good candidates for the development of new anticancer drugs.
Streptomyces sp. L79-333 배양액 유래의 항균활성물질의 분리정제
장흥배(Hung Bae Chang) 산업기술교육훈련학회 2016 산업기술연구논문지 (JITR) Vol.21 No.3
The strain designated as Streptomyces sp. L79-333 produced a water-insoluble compound, named as KP-2, which showed anti-microbial activities against several Gram-positive and negative bacteria under paper disk anti-microbial assay. The production of KP-2 in Streptomyces sp. culture broth was exarnined using several carbon and nitrogeo sources such as maltose and skim milk for their effects on growth of Streptomyces sp. as well on antibiotic activity. KP-2 was purified from the culture supernatant by 2-step silica gel column chromatography followed by solvent extraction. KP-2 represented a high pH stability under acidic and alkaline conditions showing more than 80%. Based on the studies of its spectroscopic properties using ultraviolet(UV) and infrared(IR), and fast atomic bombardment mass(FAB-Mass) spectrum analyses, KP-2 was identified as echinomycin. Echinomycin, also known as quinomycin A, is ao quinoxaline group antibiotic.
Streptomyces sp. ᄂ79-290 배양액 유래의 산성지 용성 항균활성 물질의 분리정 제
장흥배(Hung Bae Chang) 산업기술교육훈련학회 2014 산업기술연구논문지 (JITR) Vol.19 No.3
In the course of the screening for microorganism producing antibiotics, Streptomyces sp. L79-290 was discovered which produced a water-nonsoluble acidic antibiotic L79-290. By using paper disk anti-microbial assay, the productivity of antibiotic from Streptomyces sp. L79-290 was tested by several carbon and nitrogen sources such as maltose and peptone, respectively. L79-290 showed anti-microbial activities against several Gram-positive and Gram-negative bacteria. From the culture supernatant, L79-290 was purified by silicagel column chromatography follow after solvent and water extraction. In the pH stability test, L79-290 showed good stability over 80% under acidic and alkali conditions, respectively. Based on the spectroscopic measurement including ultraviolet(UV), infrared(IR), and fast atomic bombardment mass(FAB-Mass) spectrom analyses, L79-290 was identified as novobiocin. Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic that is produced by Streptomyces niveus.
Streptomyces sp. L77-140 이 생산하는 항생물질의 분리정제 및 동정
장흥배(Hung-Bae Chang) 산업기술교육훈련학회 2011 산업기술연구논문지 (JITR) Vol.16 No.4
In the course of the screening for microorganism producing antibiotics, Streptomyces sp. L77-140 was discovered which produced a water-soluble basic antibiotic L77-140. By using paper disk anti-microbial assay, the productivity of antibiotic from Streptomyces sp. L77-140 was tested by several carbon and nitrogen sources such as soluble starch and soybean flour, respectively. L77-140 showed anti-microbial activities against Gram-positive and Gram-negative bacteria. From the culture supernatant, L77-140 was purified by cation exchange chromatography and silicagel column chromatography. In the pH stability test, L77-140 was stable under acidic and alkali conditions. Based on the spectroscopic measurement including Ultra Violet(UV), In Frared(IR), Nuclear Magnetic Resonance (NMR) and mass spectrum(Mass) analyses, L77-140 was identified as spenolimycin.
Min,Tae-Jin,Chang,Hung-Bae 동국대학교 자연과학연구소 1988 자연과학연구 논문집 Vol.8 No.-
흰느타리버섯중의 단백질을 분리정체하기 위해서 AH-Sepharose 4B 겔을 출발물질로 하여 benzoyl-AH-Sepharose 4B를 합성한 다음 친화성 크로마토그래피 하였다. 합성겔의 ligand 인 benzoyl기의 capacity는 겔 1ml당 9.3 micromole이었다. 합성겔인 benzoyl-AH-Sepharose 4B에 친화성이 있는 단백질과 비친화성 단백질들의 겉보기 분자량은 각각 29.5, 31.5, 34.0, 71.0 및 89.0KD와 1.6, 4.6, 7.0, 35.0 및 61.0KD였으며 비극성, 극성, 양성 및 음성전기를 갖고있는 아미노산의 조성은 각각 45.68, 26.93, 11.81 및 15.58%와 42.56, 29.56, 12.90 및 14.98% 였다. 비교실험하기 위해 실시한 AH-Sepharose 4B겔에 의한 친화성 크로마토그래피에서 겔에 친화성이 있는 단백질들의 겉보기 분자량은 각각 3.2, 31.0 및 61.0KD였고 , 비극성, 극성, 양성 및 음성전기를 갖는 아미노산의 조성은 각각 44.05, 29.13, 13.91 및 12.91%였다. 합성겔의 hydrophobic한 ligand인 benzoyl기에 의해서 비극성 단백질들이 선택적으로 분리되었다. For selective purification of protein in Pleurotus cornucopiae(Per.) Rolland, affinity chromatography was performed by benzoyl-AH-Sepharose 4B gel synthesized using AH-Sepharose 4B with starting material. Ligand capacity of benzoyl group was 9.3micromole per milliliter of gel. Apparent molecular weights of affinity proteins eluted from benzoyl-AH-Sepharose 4B were 29.5, 31.5, 34.0, 71.0 and 89.0KD, while that of nonaffinity proteins were 1.6, 4.6, 7.0, 35.0 and 61.0KD respectively, and the contents of nonpolar, polar, positively charged and negatively charged amino acids in affinity proteins were 45.68, 26.93, 11.81 and 15.58%, while that nonaffinity proteins were 42.56, 29.56, 12.90 and 14.98% respectively. Apparent molecular weights of affinity proteins eluted from AH-Sepharose 4B were 3.2, 31.0 and 61.0KD, respectively, and the contents of nonpolar, polar, positively charged and negatively charged amino acids were 44.05, 29.13, 13.91 and 12.91%, respectively. The nnpolar proteins were selectively purified by hydrophobic ligand of benzoyl group of gel.