http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
유전독성 대체시험법(in vitro소핵시험)의 국내검증시험연구
이현걸 ( Hyun Kul Lee ),김종극 ( Jong Geuk Kim ),이우주 ( Woo Joo Lee ),맹은호 ( Eun Ho Maeng ),이종윤 ( Jong Yoon Lee ),정영신 ( Young Shin Chung ),김창환 ( Chang Hwan Kim ),김윤순 ( Yun Soon Kim ),장미해 ( Mi Hae Jang ),이석종 ( 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1
This validation study was performed to introduce and set up the in vitro micronucleus test, as an alternative to the existing chromosome aberration test, in the test facilities in Korea, and to establish the competence of each facility to perform the in vitro micronucleus test independently. The 10 coded substances, including 6 positive and 4 negative in genotoxicity assay, were tested. Six facilities were divided into two subgroups, A and B. Three coded positive compounds and two negative compounds were sent to each test facility. The compounds were tested according to the facility`s GLP system. In group A, positive results were obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. In group B, positive results were also obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. For the Urethane, an in vitro equivocal compound tested by group B, negative results were obtained. These results suggested that the compound may be effectively activated only in in vivo environment. The results show that the six test facilities are capable of performing the in vitro micronucleus test independently and producing consistent results.
연구논문 : 유전독성 대체시험법(in vitro소핵시험)의 국내검증시험연구
이현걸 ( Hyun Kul Lee ),김종극 ( Jong Geuk Kim ),이우주 ( Woo Joo Lee ),맹은호 ( Eun Ho Maeng ),이종윤 ( Jong Yoon Lee ),정영신 ( Young Shin Chung ),김창환 ( Chang Hwan Kim ),김윤순 ( Yun Soon Kim ),장미해 ( Mi Hae Jang ),이석종 ( 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1
This validation study was performed to introduce and set up the in vitro micronucleus test, as an alternative to the existing chromosome aberration test, in the test facilities in Korea, and to establish the competence of each facility to perform the in vitro micronucleus test independently. The 10 coded substances, including 6 positive and 4 negative in genotoxicity assay, were tested. Six facilities were divided into two subgroups, A and B. Three coded positive compounds and two negative compounds were sent to each test facility. The compounds were tested according to the facility`s GLP system. In group A, positive results were obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. In group B, positive results were also obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. For the Urethane, an in vitro equivocal compound tested by group B, negative results were obtained. These results suggested that the compound may be effectively activated only in in vivo environment. The results show that the six test facilities are capable of performing the in vitro micronucleus test independently and producing consistent results.
음성출력/학습기능을 지원하는 군사영어약어 자동번역 시스템 설계
김홍섭,이현걸,Kim Hong-Seop,Lee Hyeon-Geol 한국국방경영분석학회 1992 한국국방경영분석학회지 Vol.18 No.1
One of the problems we frequently face during the ROK and US Combined operations is the English Military abbreviations because they often causes a lot of confusion. Many military abbreviations we generated, changed, and disappeared, so it is very hard to figure out their meaning sometimes. This system is designed to make it easier to register, alter, and find out English abbreviations through hypermedia techniques, which is utilizing nonsequential and direct search system similar to human sensory organs. So this enables us to keep up with the latest abbreviations. It is also designed to overcome mutual communications barriers by audio-visual aids through the graphic and phonetic functions of the program, and to test users via a random selection of questions.
대장균에서 발현된 인간 Cytochrome P450 1A1과 Rat NADPH-P450 Reductase와의 Fusion Protein의 효소 특성 연구
천영진,정태천,이현걸,한상섭,노정구 한국독성학회 1996 Toxicological Research Vol.12 No.2
The enzymatic properties for NADPH-P450 reductase domain of a fusion protein between human cytochrome P450 1A1 and rat NADPH-P450 reductase expressed in Escherichia coli were investigated. The fusion plasmid pCW/1A1OR-expressed E. coli membrane showed high NADPH-cytochrome c reductase activity ($830.1\pm 85.8 nmol\cdot min^{-1}\cdot mg protein^{-1}$), while pCW control vector and P 450 1A1 expression vector pCW/1A1 showed relatively quite low activity ($4.35\pm 0.49, 3.27\pm 0.50 nmol\cdot min^{-1}\cdot mg protein^{-1}$, respectively). The kinetic curves for NADPH-cytochrome c reductase followed typical Michaelis-Menten kinetics. The $K_{max}$ and $V_{max}$ for NADPH-dependent reductase activity were $8.24\pm 2.61\mu $and $817.9\pm 60.8 nmol\cdot min^{-1}\cdot mg protein^{-1}$, respectively, whereas those for cytochrome c-dependent reductase activity were $19.97\pm 2.86\mu M$ and $1303.5\pm 67.1 nmol\cdot min^{-1}\cdot mg protein^{-1}$. The reductase activities were also compared with those of rat, porcine and human liver microsomes. The activity of pCW/ 1A1OR-expressed E. coli membrane was 15.2-fold higher than that of rat liver microsome. Treatment with benzo(a)pyrene, 7-ethoxyresorufin and $\alpha$-naphthofiavone which are known as specific substrates or inhibitor for human P450 1A1 increased NADPH-cytochrome c reductase activity of fusion protein in E. coli membrane dose-dependently. These results demonstrate that the membrane topology of fused enzyme may be important for activity of its NADPH-P450 reductase domain.
Salmonella typhimurium Strain TA98, 100에서 감잎차, 녹차, 우롱차 추출물의 돌연변이 억제효과
송현순(Hyun-Soon Song),이현걸(Hyun-Kul Lee),강명희(Myung-Hee Kang) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.3
실제 음용 조건으로 추출한 한국산 감잎차, 녹차, 우롱차 추출물의 돌연변이 억제효과를 보기 위하여 간접 돌연변이 물질로 polycyclic aromatic hydrocarbon인 B[a]P, heterocyclic amine인 IQ, aromatic amine인 2-AA, 그리고 직접 돌연변이 물질인 4-NQO에 대하여 salmonella/microsome assay를 응용하여 실험하였다. 실험 결과, 음용하는 조건으로 추출한 감잎차, 녹차, 우롱차 추출물에는 간접 돌연변이 물질에 의한 colony 생성에 미치는 돌연변이 억제 효과가 탁월함을 볼 수 있었다. 간접 돌연변이 물질 중 B[a]P이나 IQ의 경우는 차 추출물의 농도 증가에 따라 돌연변이 물질에 대하여 농도 의존적으로 colony 생성을 억제시키었으나 2-AA의 경우는 저농도 첨가시 co-mutagenic하게 작용하여 colony 수를 증가시키다가 고농도 첨가시 colony 생성을 억제시킴을 보였다. 이와는 달리 직접 돌연변이 물질인 4-NQO의 경우는 저농도 첨가시에는 큰 변화가 없다가 고농도 첨가시 colony 생성을 더 증가시키는 결과를 보였다. 돌연변이 억제효과의 작용 양상을 보면, 각 차 추출물에 따른 차이는 크게 볼 수 없었으며 TA98과 TA100 등 균주에 따른 경향의 차이도 볼 수 없었으나 돌연변이 물질의 종류에 따라서는 많은 차이를 보였다. 본 실험 결과 실제 음용하는 조건으로 추출한 한국산 감잎차, 녹차, 우롱차 추출물이 간접 돌연변이 물질들에 대하여 억제효과를 나타내는 것을 확인할 수 있었으며, 직접 돌연변이 물질에 대해서는 억제효과를 볼 수 없었다. 이 결과로부터 본 연구에서 사용한 차 추출물의 돌연변이 억제효과 기전은 각 차 추출물들이 돌연변이 물질과 직접 결합하여 돌연변이를 억제한다기보다는 돌연변이 물질의 체내 활성화 대사과정을 억제하는 물질로 작용하는 것으로 생각되었다. Water extracts of persimmon leaf tea(PLTE), green tea(GTE) and oolong tea(OTE), at the concentration used for human consumption, were examined for inhibitory effects on the mutagenicity of major classes of dietary and environmental mutagens including indirect-acting mutagens, B[a]P(benzo[a]pyrene), IQ(2-amino-3-methylimidazo[4,5-f]quinoline), 2-AA(2-aminoanthracene) in the presence of S9 mix and direct-acting mutagen, 4-NQO(4-nitroquinoline-1-oxide) without S9 mix, using the modified Ames Salmonella/microsome assay. PLTE, GTE and OTE showed very potent and concentration-dependent antimutagenic effects against indirect-acting mutagens B[a]P and IQ. At the maximum concentration(16,200㎍/plate) of each tea extract, number of colonies decreased in a dose dependent manner up to 82~100%. Similar inhibition of PLTE, GTE and OTE were seen at higher concentration in the mutagenicity of the 2-AA following an initial increase in the activity at lower concentration. However, the mutagenicity of the direct-acting mutagen 4-NQO were not suppressed at lower concentration of the three tea extracts, and higher concentration of the tea extracts enhanced mutagenic activity of the mutagen. There were no differences in the mode of antimutagenesis between PLTE, GTE, and OTE, in both Salmonella typhimurium TA98 and TA100 strains against the same mutagen. In conclusion, the water extracts of persimmon leaf tea, green tea and oolong tea possess marked antimutagenic potential against a variety of important dietary and environmental indirect-acting mutagens, but the activity was not observed against the direct-acting mutagens. These results suggest that the mode of inhibitory action may not have resulted from direct interaction between tea extracts and the mutagens, but rather from indirect metabolic inactivation of mutagens by tea extracts.