사군자탕(四君子湯)의 항염증 효능 연구

이진아,하혜경,정다영,이호영,이남헌,이준경,서창섭,김정훈,이미영,신현규,Lee, Jin-Ah,Ha, Hye-Kyung,Jung, Da-Young,Lee, Ho-Young,Lee, Nam-Hun,Lee, Jun-Kyoung,Seo, Chang-Seob,Kim, Jung-Hoon,Lee, Mee-Young,Shin, Hyeun-Kyoo 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

Objectives : To provide the information of efficacy for Sagunja-tang (Sijunzi-tang; SG), it was evaluated the anti-inflammatory effect. SG, a widely used herbal formula in tranditional Korean medicine, has been used to treat for the Boki-invigorating. In many studies, plant-derived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of SG extract on LPS-stimulated RAW 264.7 cells. Methods : The productions of nitric oxide (NO), prostaglandin (PG)$E_2$, interleukin (IL)-6 and tumor necrosis factor (TNF)-$\alpha$ were examined in a macrophage cell line, RAW 264.7 cells, in the presence of the SG extract. RAW 264.7 cells were incubated with LPS $1\;{\mu}g/mL$ and SG extract for 18 hours. The anti-inflammatory activity of SG was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 0, 2 and 4 hours following carrageenin-induced paw edema in rats. Results : SG extract showed inhibitory effect on $PGE_2$, IL-6 and TNF-$\alpha$ by LPS-stimulated RAW 264.7 cells. But SG extract was not inhibitory effect on NO by LPS-stimulated RAW 264.7 cells. And administration of SG extract (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : These results suggest that SG extract has anti-inflammatory activities in vitro and in vivo models.

말기암환자에서 혈장 내독소 농도가 생존기간에 미치는 영향

이진아,윤호민,최윤선,연종은,이준영,Lee, Jin-Ah,Yoon, Ho Min,Choi, Youn Seon,Yeon, Jong Eun,Lee, June Young 한국호스피스완화의료학회 2014 한국호스피스.완화의료학회지 Vol.17 No.2

목적: 말기암환자의 여명 예측은 치료의 이득과 위해를 판단하는 잣대가 되고, 적절한 의료 중재 제공 및 환자의 자율성에 기초한 의사결정에 중요한 기준이 된다. 특히 많은 수의 말기암환자는 다발성 장기 부전으로 사망에 이르기 때문에 본 연구에서는 이를 반영할 수 있는 혈장 내독소 농도와 생존기간과의 연관성을 알아보고자 하였다. 방법: 2009년 4월부터 10월까지 6개월 동안, 만 20세 이상의 말기암환자 56명을 대상으로 혈장 내독소 농도를 측정하고, 생존기간을 조사하였다. 나이, 성별, 원발 암 부위, 암 치료 경력, 전이여부, 투약상황 및 활력 증후, Karnofsky 수행지수 등의 신체검사, 백혈구 수, 혈색소, 적혈구용적률, aspartate aminotransferase, alanine aminotransferase C-반응성 단백질, 총 빌리루빈, 내독소 등의 혈액검사를 시행하였다. 결과: 혈장 내독소 농도, 성별, 나이, 백혈구 수, 혈색소, 적혈구 용적률, AST, ALT, 총 빌리루빈, C-반응성 단백질, 통증강도를 단변량 분석한 결과 혈장 내독소 농도는 생존기간과 통계적으로 유의한 관계를 보이지는 않았으나, 단변량 분석에서 생존기간에 유의한 영향을 미치는 성별, 백혈구 수, 총 빌리루빈을 포함하여 시행한 다변량 분석에서 혈장 내독소 농도는 생존기간과 통계적으로 유의한 관계가 있는 것으로 나타났다. 결론: 다발성 장기 부전과 관련이 있는 혈장 내독소 농도는 생존기간 예측인자로서 쓰일 수 있는 가능성을 보여 주었다. Purpose: Since most terminally ill cancer patients die of multiple organ failure, plasma endotoxin concentration levels may be used to predict the life expectancy. This study was performed to evaluate the clinical significance of endotoxin level in plasma as a prognostic factor for survival in patients with terminal cancer. Methods: This study was conducted with 56 terminally ill cancer patients, above 20 years old, from April 2009 through October 2009. Demographic characteristics, Karnofsky performance status, and survival time were evaluated. We analyzed blood levels of white blood cell hemoglobin, hematocrit, aspartate aminotransferase, alanine aminotransferase, c-reactive protein, total bilirubin and endotoxin in each patient. Results: We considered following variable for univariate analysis: plasma endotoxin level, sex, age, WBC, hemoglobin, hematocrit, AST, ALT, total bilirubin, CRP and severity of pain. Univariate analysis did not show a significant association between plasma endotoxin level and survival time. However, in a multivariate analysis with factors that were found to be significantly associated with survival sex, WBC count and total bilirubin level in univariate analysis, high levels of plasma endotoxin and short survival time were significantly related. Conclusion: Plasma endotoxin level could be used as a prognostic factor to predict the life expectancy of terminally ill cancer patients.

대황-황금 혼합물이 만성 역류성 식도염에 미치는 효과

이진아,오민혁,신미래,노성수,박해진,Lee, Jin A,Oh, Min Hyuck,Shin, Mi-Rae,Roh, Seong-Soo,Park, Hae-Jin 한국생약학회 2022 생약학회지 Vol.53 No.1

면역 기능이 정상인 소아에서 발생한 b형 Haemophilus influenzae에 의한 협부 봉와직염(Buccal Cellulitis) 1례

이진아,김동호,구자욱,이환종,Lee, Jin A,Kim, Dong Ho,Koo, Ja Wook,Lee, Hoan Jong 대한소아감염학회 2001 Pediatric Infection and Vaccine Vol.8 No.2

어린 소아에서 상기도 감염의 증상이 선행한 후 수 시간 내에 급격히 진행하며 고열을 동반하는 적보라빛의 협부 및 경부 부종이 있는 경우, 반드시 H. influenzae에 의한 감염을 고려하여야 하며, 균혈증 및 무증상적 수막염이 동반될 가능성이 크므로 혈액배양검사 및 뇌척수액 천자를 반드시 시행하여야 한다. 예방접종 시행 전 서구의 경우, 봉와직염이 Hib에 의한 침습성 질환 중 수막염, 후두염 등에 이어 5번째를 차지하며, 전체적으로 2~15%를 차지할 정도로 그 빈도가 높은 것으로 알려져 있으나 우리나라에서는 매우 드물게 발견된다. 저자들은 면역기능에 이상이 없었던 18개월 여아에서 상기도 감염의 증상 및 고열이 선행한지 수 시간 후 급격히 진행하는 적보라빛의 협부 봉와직염으로, Hib에 의한 균혈증이 동반되었고, 2주간 3세대 cephalosporin의 투여로 호전되었던 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Buccal cellulitis which presents with high fever and a swelling of the cheek with violaceous hue in young children is most often caused by H. influenzae. Bacteremia is common in buccal cellulitis caused by H. influenzae, and a culture of cerebrospinal fluid should be obtained because meningitis may be present despite the lack of meningeal irritation signs. Although buccal cellulitis is considered to be one of the important manifestations of H. influenzae infection, only two cases have been reported in Korea yet. We experienced a case of buccal cellulitis with H. influenzae bacteremia in an immunocompetent girl of 18-month-old. She was presented with high fever followed by rapidly progressive swelling and tenderness of both cheeks with violaceous hue in four hours. The blood culture revealed H. influenzae type b. There was no concurrent otitis media, sinusitis, or meningitis and no portal of entry was identified. Fever subsided two days after starting intravenous cefotaxime. Intravenous cefotaxime was subsequently changed to oral cefixime, and antibiotics were administered for a total of two weeks. We report this case with a review of related literature.

사상 체질 처방의 항염증 효능 비교 연구

이진아,하혜경,이호영,정다영,이준경,황대선,신현규,Lee, Jin-Ah,Ha, Hye-Kyung,Lee, Ho-Young,Jung, Da-Young,Lee, Jun-Kyoung,Huang, Dae-Sun,Shin, Hyeun-Kyoo 사상체질의학회 2010 사상체질의학회지 Vol.22 No.4

1. Objectives 4 herbal formulas (Yanggyeoksanhwa-tang, Yeoldahanso-tang, Cheongsimyeonja-tang and Taeeumjowi-tang) were applied to investigate the anti-inflammatory activities. In many studies, plant-derived anti-inflammatory efficacies have been investigate for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory activities of 4 herbal formulas on LPS-stimulated RAW 264.7 cells. 2. Methods The productions of nitric oxide (NO), prostaglandin (PG)$E_2$, interleukin(IL)-6 and tumor necrosis factor (TNF)-${\alpha}$ were examined in the presence of the 4 herbal formulas in RAW 264.7 cells. The cells were incubated with LPS 1 ${\mu}g/mL$ and 4 herbal formulas for 18 hrs. The anti-inflammatory activity of 4 herbal formulas were investigate by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hrs following carrageenininduced paw edema in rats. 3. Results Yanggyeoksanhwa-tang and Cheongsimyeonja-tang showed inhibitory effect on $PGE_2$ production in LPS-stimulated RAW 264.7 cells and a reduction in carrageenin-induced paw edema on rats. Yanggyeoksanhwa-tang showed inhibitory effect on IL-6 in LPS-stimulated RAW 264.7 cells. 4 herbal formulas not affect on NO and TNF-${\alpha}$ inhibition in LPS-stimulated RAW 264.7 cells. 4. Conclusions These results suggested that Yanggyeoksanhwa-tang and Cheongsimyeonja-tang have anti-inflammatory activity.

點眼藥에 대한 文獻的 考察

이진아,김현아,이원철,Lee, Jin-A,Kim, Hyeon-A,Lee, Won-Cheol 대한한방안이비인후피부과학회 1993 한방안이비인후피부과학회지 Vol.6 No.1

소아의 임상 검체 및 건강한 소아의 비인두에서 분리된 폐구균의 혈청형 및 페니실린 감수성

이진아,김남희,김동호,박기원,김윤경,김경효,박진영,최은화,이환종,Lee, Jin-A,Kim, Nam-Hee,Kim, Dong-Ho,Park, Ki-Won,Kim, Yun-Kyung,Kim, Kyoung-Hyo,Park, Jin-Young,Choi, Eun-Hwa,Lee, Hoan-Jong 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.9

목 적 : 폐구균은 건강한 소아의 비인두에서 분리될 수 있는 정상 균총이지만, 다양한 종류의 침습성 감염과 국소 감염을 일으킨다. 최근에 영아 및 어린 소아에서도 침습성 감염에 대한 예방 효과가 우수한 7가의 단백 결합 백신이 개발되었다. 백신의 적절한 사용을 위해서는 백신을 사용하고자 하는 지역에서 분리되는 폐구균의 혈청형에 대한 분석이 필요하다. 방 법 : 2000년 3월부터 2002년 2월까지 2년간 서울대학교 어린이병원, 이화여자대학교 동대문병원, 성균관대학교 강북삼성병원에 내원한 15세 미만의 환아의 임상 검체에서 분리된 폐구균 127 균주와 1997년 11월부터 1998년 10월까지 1-5세 사이의 건강한 소아의 인후 도찰 검체에서 분리된 폐구균 72균주를 대상으로 Quellung reaction을 이용하여 혈청형을 결정하였으며, oxacillin 디스크 확산법을 이용하여 penicillin에 대한 감수성을 검사하였다. 결 과 : 임상 검체에서 폐구균이 분리된 소아의 연령은 2세 미만이 43%, 2-5세가 35%, 5-15세가 21%였다. 균혈증, 뇌수막염, 복막염 등의 침습성 감염은 전체 감염의 24%를 차지하였으며 이러한 경우 폐구균의 페니실린 내성률은 90%였다. 2세 미만의 경우는 전체 폐구균 감염의 22%가 침습적 감염이었고 호흡기계 검체 및 중이염은 각각 46%, 26%를 차지하였다. 폐구균의 혈청형 분포는 19F(24%), 19A(17%), 23F(15%), 6A(11%), 6B(7%), 9V (6%)의 순이었으며, 페니실린 내성빈도는 94%였다. 임상 검체에서 분리된 폐구균 중 56%(교차반응 혈청형 포함시 84%), 침습성 감염과 중이염의 경우 각각 57%(80%), 48%(88%)가 7가 백신에 포함되었다. 건강한 소아의 인후두 도찰 검사의 폐구균 혈청형은 19F(31%), 14(21%), 11A(13%), 23F(11%), 18C(6%), 19A(4%)의 순으로 이 6가지 혈청형이 전체의 86%를 차지하였고 페니실린 내성률은 86%였으며 7가 백신에 71%(78%)가 포함되었다. 임상 검체 및 건강한 소아에서 분리된 폐구균 중 페니실린 내성균의 60%(90%)가 7가 백신에 포함되었다. 결 론 : 우리나라 소아의 임상 검체 및 건강한 소아의 비인두에서 분리되는 폐구균의 혈청형은 6A, 6B, 14, 19A, 19F, 23F 등이 대부분을 차지하였으며, 분리된 균주의 56-84%가 7가 단백 결합 백신에 포함되었다. Purpose : Pneumoccocus is one of the most important causes of invasive infection through the childhood period and the prevelance of antibiotics resistance of pneumococcus is increasing worldwide. A 7-valent conjugate vaccine has been developed. It is important to know the prevalence of each serotype of pneumococci in the countries where the vaccine is used to estimate the coverage rate by the vaccine. Methods : One hundred and twenty seven strains of clinical isolates and 72 strains from healthy carriers recovered from Korean children during the period from 1997 to 2002 were subjected to determination of serotype by Quellung reaction and penicillin susceptibility with oxacillin disc diffusion test. Results : Forty-three per cent of clinical isolates were obtained from children under two years of age. Thirty strains(24%) were isolated from normally sterile body fluids. The frequent serotypes were 19F, 19A, 23F, 6A, 6B and 9V. Fifty-six per cent of the clinical isolates were represented in the current 7-valent protein conjugate pneumococccal vaccine, and 84% when the cross-reactive serotypes were included. Frequent serotypes of strains isolated from one to five year-old healthy children were 19F, 14, 11A, 23F, 18C, and 19A. Seventy-one per cent of the carrier strains were included in the 7-valent vaccine. Ninety-three per cent of the clinical isolates and 86% of carrier strains were not susceptible to penicilline. Conclusion : Fifty-six to 84% of pneumococci recovered from Korean children are covered by the current 7-valent protein conjugate pneumococcal vaccine and the prevalence of penicillin resistance was very high.

과학기술위성3호 부탑재체 소형영상분광기 미광 해석

이진아,이준호,Lee, Jin Ah,Lee, Jun Ho 한국광학회 2012 한국광학회지 Vol.23 No.4

This paper reports on the stray light analysis results of a compact imaging spectrometer (COMIS) for a microsatellite STSAT-3. COMIS images Earth's surface and atmosphere with ground sampling distances of 27 m at the 18~62 spectral bands (0.4 ~ 1.05 ${\mu}m$) for the nadir looking at an altitude of 700 km. COMIS has an imaging telescope and an imaging spectrometer box into which three electronics PCBs are embedded. The telescope images a $27m{\times}28km$ area of Earth surface onto a slit of dimensions $11.8{\mu}m{\times}12.1mm$. This corresponds to a ground sampling distance of 27 m and a swath width of 28 km for nadir looking posture at an altitude of 700 km. Then the optics relays and disperses the slit image onto the detector thereby producing a monochrome image of the entrance slit formed on each row of detector elements. The spectrum of each point in the row is imaged along a detector column. The optical mounts and housing structures are designed in order to prevent stray light from arriving onto the image and so deteriorating the signal to noise ratio (SNR). The stray light analysis, performed by a non-sequential ray tracing software (LightTools) with three dimensional housing and lens modeling, confirms that the ghost and stray light arriving at the detector plane has the relative intensity of ${\sim}10^{-5}$ and furthermore it locates outside the concerned image size i.e. the field of view of the optics. 과학기술위성3호 부탑재체인 COMIS(Compact Imaging Spectrometer)는 무게 4.25 kg의 소형영상분광기로써 지표면 및 대기과학을 연구할 목적으로 개발되었다. COMIS는 지표면, 대기, 수면으로부터 반사되는 태양 에너지를 고도 700 km에서 가시광 및 근적외선 영역(0.4 ~1.05 ${\mu}m$)에서 해상도 27 m, 관측폭 28 km, 파장 분해능 2 ~ 15 nm를 갖도록 결상 광학계와 분광 광학계로 구성된다. 먼저 지상 $27m{\times}28km$의 해당되는 지표면에서 반사된 빛은 COMIS의 결상 광학계에 의하여 상면에 위치한 $11.8{\mu}m{\times}12.1mm$의 선형 슬릿으로 맺히고, 이후 약 1.1배의 배율을 갖는 분광 광학계의 상면에 $1.4mm{\times}13.3mm$의 크기를 갖는 분광 스펙트럼 영상으로 나타난다. 이 때 부분 반사 및 산란 등에 의한 신호대잡음비(SNR, signal to noise ratio) 저하를 방지하기 위하여 렌즈 및 거울 면에 무반사 및 고반사 코팅이 적용되었고, 내부에는 미광 차단 구조물 등이 설치되었다. 미광 차단 설계의 적정성을 확인하기 위하여 미광 모델 수립과 상용 프로그램을 이용한 미광 해석을 수행하였다. 해석 결과 미광의 세기는 정상 결상 대비 무시할 정도의 값($10^{-5}$)으로 영상에는 실질적인 영향이 없음을 확인하였다.

목과(木瓜)추출물이 급성 간손상 흰쥐에 미치는 효과

이진아,신미래,이지혜,노성수,Lee, Jin A,Shin, Mi-Rae,Lee, Ji Hye,Roh, Seong-Soo 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.1

Objective: This study was conducted to investigate the effect of Chaenomelis Fructus (CF) water extract on thioacetamide (TAA)-treated rats. Methods: Rats were divided into five groups: one normal group (n=8) and four with TAA-induced hepatic injury. These treatment groups were administered distilled water (n=8); silymarin 100 mg/kg (n=8); CF 100 mg/kg (n=8); and CF 200 mg/kg (n=8). In the TAA groups, the acute liver injury was induced via IP injection (200 mg/kg), and the silymarin and CF extract were then orally administered for three days. Subsequently, serum levels of GOT, GPT, and ammonia were confirmed as well as protein expressions using liver tissue. Results: In the liver injury-induced rats, CF administration reduced tissue damage and serum levels of GOT, GPT, and ammonia. In addition, CF increased the anti-oxidant proteins Nrf2, Keap1, HO-1, and catalase and significantly regulated matrix metalloproteinases (MMP-2 and MMP-9) and their tissue inhibitors (TIMP-1 and TIMP-2). Conclusions: In this animal model of liver injury induced by TAA, CF extract is determined to have a hepatoprotective effect by increasing anti-oxidant proteins that relieve damage and by regulating the expression of matrix metalloproteinases.

애엽(艾葉) 추출물이 알코올성 위염에 미치는 효과

이진아,서정복,김진영,신미래,박해진,노성수,Lee, Jin A,Seo, Jeong Bok,Kim, Jin Young,Shin, Mi-Rae,Park, Hae-Jin,Roh, Seong-Soo 대한한방내과학회 2022 大韓韓方內科學會誌 Vol.43 No.4

Objective: Alcohol is known to cause inflammation in the stomach by decreasing the protective substances of the gastric mucosa and increasing oxidative stress. The purpose of this study was to investigate the anti-inflammatory effect of Artemisiae Argyi Folium extract (AF) on alcohol-induced gastritis. Methods: The total polyphenol and flavonoid contents of AF were confirmed through an in vitro experiment. Radical scavenging activities were confirmed using DPPH and ABTS assays. In vivo experiments were conducted on mice divided into 5 groups (n=8): a normal group (Nor), an alcohol-induced gastritis group (Con), an alcohol-induced gastritis group administered 10 mg/kg sucralfate (SC), an alcohol-induced gastritis group administered 100 mg/kg AF (AFL), and an alcohol-induced gastritis group administered 200 mg/kg AF(AFH). The serum levels of reactive oxygen species (ROS) were determined, and protein expressions were confirmed in gastric tissues. Results: In alcohol-induced gastritis, AF alleviated damage to the gastric mucosa caused by alcohol. AF also decreased the serum ROS levels. Western blots showed that AF decreased the expression of NADPH oxidase and decreased the expression of the NF-κB pathway associated with inflammation. AF also decreased the expression of adhesion molecules and chemokine proteins, and increased the expression of anti-inflammatory proteins. Conclusions: AF not only reduced oxidative stress in alcohol-induced gastritis, but it also relieved gastric mucosal inflammation by regulating the expression of the NF-κB pathway.