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우관식,황인국,노영희,정헌상,Woo, Koan-Sik,Hwang, In-Guk,Noh, Young-Hee,Jeong, Heon-Sang 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.6
국산 감초를 처리온도, 시간 및 첨가수분함량을 변수로하여 열처리한 다음 에틸아세테이트와 에탄올로 추출하여 성분 및 생리활성 변화를 분석하고 반응표면분석으로 최적화 조건을 조사하였다. 에틸아세테이트 추출물의 추출수율은 $130^{\circ}C$, 3시간, 가수량 50%일 때 10.90%로 무처리구의 0.80%보다 높았으며, 에탄올 추출물은 $120^{\circ}C$, 2시간, 가수량 20%로 처리한 시료가 25.00%로 무처리구의 15.80%보다 높았다. 총 폴리페놀 함량은 열처리구가 무처리구보다 높았으며, 에탄올 추출물의 $120^{\circ}C$, 2시간, 가수량 20% 처리구가 845.67 mg/100 g으로 무처리구의 277.00 mg/100 g보다 높았다. 항산화활성($IC_{50}$)은 열처리구가 무처리구보다 높게 나타났으며, 에틸아세테이트 추출물 $120^{\circ}C$, 2시간, 가수량 20% 처리구에서 0.53 g/L로 무처리구의 12.34 g/L보다 매우 높게나타났다. 총항산화력(AEAC)은 모든 처리구에서 무처리구보다 높게 나타났는데 에틸아세테이트 추출물 $120^{\circ}C$, 2시간, 가수량 40% 처리구에서 1,584 mg AA eq로 무처리구의 1,263 mg AA eq보다 높게 나타났다. 본 연구결과 감초의 항산화활성, 총 폴리페놀 함량 등을 증가시키기 위한 최적의 열처리 조건은 $120^{\circ}C$, 2시간, 가수량 $20{\sim}40%$로 판단되었다. Antioxidative activity and polyphenol contents of heated licorice in Korea extracted by ethyl.acetate (EtOAc) and ethyl.alcohol (EtOH) were evaluated at various heating temperatures (110, 120, 130, 140, and $150^{\circ}C$), times (1, 2, 3, 4, and 5 hr), and moisture contents (10, 20, 30, 40, and 50%). Maximum extraction yields of EtOAc extract was 10.9% at $130^{\circ}C$, 3 hr, and 50% moisture content and that of EtOH extract was 25.0% at $120^{\circ}C$, 2 hr, and 20% moisture content, whereas those of control were 0.8 and 15.8%, respectively. The highest total polyphenol content was 845.67 mg/100 g in EtOH extract at $120^{\circ}C$, 2 hr, and 20% moisture content (control: 277.00 mg/100 g). The antioxidative activity ($IC_{50}$) was the highest value of 0.53 mg/mL in EtOAc extract at $120^{\circ}C$, 2 hr, and 20% moisture content (control: 12.34 mg/mL). The highest ascorbic acid equivalent antioxidant activity value of 1,584 mg ascorbic acid (AA) eq was obtained from EtOAc extract at $120^{\circ}C$, 2hr, and 40% moisture content (control: 1,263 mg AA eq). Optimum heating conditions for the improvement of antioxidative activity of licorice in Korea was $120^{\circ}C$, 2 hr, and $20{\sim}40%$ moisture content.
논 재배 배수방법이 팥 에탄올 추출물의 항산화성분 및 항산화활성에 미치는 영향
우관식,정기열,송석보,고지연,이재생,최영대,윤을수,정태욱,오인석 한국작물학회 2014 한국작물학회지 Vol.59 No.3
This study investigated the changes of antioxidantcompounds and antioxidant activity of adzuki bean bydrainage methods in poorly drained sloping paddy field. The soil moisture contents of under pipe and open ditchdrainage on very poorly drained paddy soil were 18.52±4.58and 19.01±4.25%, and imperfectly drained paddy soil were14.87±4.82 and 18.64±3.85%, respectively. Moisture, protein,fat and ash contents of adzuki bean with drainage methodswere 10.10~11.60, 14.13~21.75, 0.02~0.73 and 2.81~3.45g/100 g, respectively. The total polyphenol, flavonoid andtannin contents, and radical scavenging activity of adzukibean showed significant differences by drainage methods. The total polyphenol, flavonoid, and tannin contents by drainagemethods were 2.73~4.14 mg GAE/g, 1.07~1.43 mg CE/g,and 1.27~1.84 mg TAE/g, respectively. The DPPH andABTS radical scavenging activities were 2.84~4.47 and5.11~6.74 mg TE/g, respectively. The antioxidant compoundsand radical scavenging activity of the adzuki bean by drainagemethods were frequently affected soil water.
우관식,김현주,김미정,심은영,고지연,이춘기,전용희 한국작물학회 2017 한국작물학회지 Vol.62 No.4
This study evaluated the quality characteristics, polyphenolic compounds, and radical scavenging activity of cooked-rice added to commercially available mixed grains. L-value of cooked-rice with various mixed grains decreased compared to that of cooked-white rice; however, a- and b-values increased. Hardness and elasticity of cooked-rice added to various mixed grains were significantly lower in the pressure cooker compared to the electric cooker. There was no significant difference in adhesiveness and stickiness between rice from the electric cooker and pressure cooker. Total polyphenol and flavonoid contents of cooked-rice added to various mixed grains were significantly increased. The average total polyphenol content of cooked-rice added to various mixed grains cooked in an electric cooker and pressure cooker were 16.50 ± 3.86 and 15.88 ± 3.52 mg gallic acid equivalent /100 g, and flavonoid contents were 1.58 ± 0.00 and 1.55 ± 0.02 mg catechin equivalents/100 g, respectively. The average of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was 9.27 ± 2.62 and 8.72 ± 2.41 mg trolox equivalent (TE)/100 g, and 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was 22.89 ± 4.60 and 23.07 ± 4.49 mg TE/100 g for cooked-rice added to various mixed grains cooked in an electric cooker and pressure cooker, respectively. Phenol content and radical scavenging activity of cooked rice was in proportion to the amount of added grains, such as brown rice, colored rice, barley, soybean, and sorghum. 본 연구에서는 국내 시중에서 유통되는 있는 혼합잡곡 제품을 첨가하여 취반 후 품질특성과 페놀성분 함량, radical 소거활성을 검정하여 추후 소비자의 수요와 용도별 잡곡 혼합비율 설정을 위한기초자료로 활용하고자 하였다. 시중 유통 혼합잡곡 제품 첨가 취반 밥의 명도는 백미에 비해 감소하였으며, 적색도와 황색도는 증가하는 경향을 보였다. 경도와 탄력은 압력밥솥에서 유의적으로 낮았으며, 부착성과 찰기는 일반밥솥과 압력밥솥에서 유의적인 차이가 없었다. 혼합잡곡 첨가에 따라 총 폴리페놀 및 플라보노이드 함량은 유의적으로 증가하였으며, 시중유통 혼합잡곡 첨가 취반 밥 에탄올 추출물의 총 폴리페놀 함량은 일반밥솥과 압력밥솥에서 각각 16.50±3.86 및 15.88±3.52 mg GAE/100 g이었고 총플라보노이드 함량은 각각 1.58±0.00 및 1.55±0.02 mg CE/100 g이었다. 일반밥솥과 압력밥솥으로 취반한 혼합잡곡 첨가 취반 밥 에탄올 추출물의 DPPH radical 소거활성은 각각 9.27± 2.62 및 8.72±2.41 mg TE/100 g으로 일반밥솥으로 취반한경우가 유의적으로 약간 높았다. ABTS radical 소거활성은각각 22.89±4.60 및 23.07±4.49 mg TE/100 g으로 압력밥솥으로 취반한 경우가 약간 높았으나 유의적인 차이는 없었다. 현미, 유색미, 보리, 콩, 수수 등의 잡곡 함유율이 높을수록제품의 페놀 성분 함량과 radical 소거활성이 높게 나타났다.
우관식,김미정,김현주,이지해,이병원,정건호,이봉규,김수림 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.3
The phenolic compounds and radical scavenging activity of ethanolic extracts from maize at various roasting conditions were evaluated in this research. The free sugar contents in roasted maize significantly decreased with higher roasting temperature and longer roasting time. The total polyphenol and total flavonoid contents in roasted maize significantly increased with higher roasting temperature and longer roasting time. The predominant phenolic acid in the roasted maize was homogentisic acid. The contents of homogentisic acid and myricetin in roasted maize significantly increased with higher roasting temperature and longer roasting time. The DPPH and ABTS radical scavenging activities of roasted maize significantly increased with higher roasting temperature and longer roasting time. DPPH and ABTS radical scavenging activities were positively correlated with phenolic compounds. The activities of these components increased following heat treatments because of the low molecularization effects of the heating process, which resulted in active, lowmolecular- weight components that were readily extracted.