토론을 활용한 진로프로그램이 고등학교 동아리 학생의 진로성숙도에 미치는 영향

고지연 인천대학교 사회과학연구원 2016 사회과학연구 Vol.9 No.-

The purpose of this study is utilizing this career program is to investigate the effect of discussions on the career maturity of high school student clubs. In addition, we analyzed how to improve, perform and plan the carreer program taking advantage of the discussion through interviews. 21st century knowledge-based society has more to watch the game as well as select the amount of knowledge and knowledge for their own purposes by creating a new paradigm that requires configuration. Therefore, based on the career program it is decided to put the debate in the process. Eventually, course of the student is a matter of choice and always have to worry. For the subjects it was selected 12 students who did not decided their courses from the ‘S’ high school freshman in the Gyeonggi S-city, And quantitative research and interviews were designed using the test and study combined qualitative analysis of observation. The plan established from March 2015, and up to six hours per week were planning to target the experimental group from August 2015 to November 2015 conducted a program of 10 sessions within the school. All sessions were conducted with 100 minutes per session, from 6:00 pm until 8:30 during 50 minutes. Career progression to a program utilizing discussion which were the result of a study conducted in the way and procedures for the experimental group are as follows: The hypothesis of that Career Programs utilizing the debate affects the career maturity of high school students' research was that there is a significant difference because the probability is significant at the 95% confidence level, p=0.002<0.05. As a result of corresponding sample T tests, the sum total of pre-career maturity scores 3.441 points, total score was improved as 3.752 points, which was statistically significant (t = - 4.12, p <0.05). Therefore, a career program utilizes the discussion to a positive impact on career maturity, and discussion showed that the map is a useful way as a career guidance.

논토양에서 경운 및 무경운재배시 재배방법별 메탄 배출 양상

고지연,이재생,김민태,강항원,강위금,이동창,신용광,김건엽,이경보,Ko, Jee-Yeon,Lee, Jae-Saeng,Kim, Min-Tae,Kang, Hang-Won,Kang, Ui-Gum,Lee, Dong-Chang,Shin, Yong-Gwang,Kim, Kun-Yeop,Lee, Kyeong-Bo 한국토양비료학회 2002 한국토양비료학회지 Vol.35 No.4

Field experiments were conducted to investigate the effect of various cultural practices on methane($CH_4$) emission in tillage and no-tillage practice in a clayey paddy soil from 1998 to 2000. The factors evaluated in tillage and no-tillage methods were types of nitrogen fertilizers, application method of chemical fertilizers, rice straw application and cultivation method. Of the nitrogen fertilizers, the amount of $CH_4$ emission in ammonium sulfate plot was the lowest, regardless of tillage and the application method. 26.4~41.1% of reduction by ammonium sulfate compared with urea. But in no-tillage which have problem of poor rice yield than tillage, coated urea was more effective nitrogen fertilizer because that showed similar $CH_4$ emission and highest rice yield at 80% of dosage of nitrogen. No-tillage plot emitted lower $CH_4$ than tillage plot where the fertilizers were incorporated. On the contrary, no-tillage plot showed a little higher $CH_4$ emission compared with tillage plot for the surface application. When rice straw was applied, no-tillage practice reduced methane emission by 26.6% compared with tillage practice, but showing a little difference of 10.7% in no application. With cultivation method, no-tillage practice reduced methane emission 26.6% compared with tillage for the 30-d-old seedling transplanting. But for the dry direct seeding practice, no-tillage was a less effective because considerable amounts of rice straw incorporated by tillage were more decomposed aerobically in the soil and emitted as $CO_2$ to the atmosphere with flooding in no-tillage soil.

경도의 방광요관역류가 있는 소아에서 99mTc-dimercaptosuccinic acid 신 스캔의 필요성

고지연,구자욱 대한소아청소년과학회 2006 Clinical and Experimental Pediatrics (CEP) Vol.49 No.6

Objective : 99mTc-dimercaptosuccinic acid(DMSA) scan is considered to be the most sensitive examination for detection of renal scars. However, because of its high radiation exposure to the kidney and its limited usefulness for patients with low grade vesicoureteral reflux(VUR), some authors have suggested that DMSA scans should be reserved primarily for children with VUR grade 3 and above. The aim of this study was to reevaluate the necessity of DMSA scans as a screening test in infants without reflux or with low grade reflux. Methods : In this retrospective study, 189 infants(mean age : 6.2 months) diagnosed as UTI were enrolled. Voiding cystourethrogram(VCUG), DMSA scan and renal ultrasonography were performed within 1 month of UTI. VUR grade was classified into three subgroups; low grade(grade 1-2), moderate grade(grade 3), and high grade(grade 4-5), respectively. Results : Renal defects were present in 67 of 189 infants, and 82 of the 378 renal units. The incidence of renal defects was significantly correlated with VUR grade(P<0.01); 28 percent without reflux, 38 percent with low grade, 53 percent with moderate grade, 100 percent with high grade, respectively. However, there was no significant differences in incidence of renal defects between the low grade and moderate grade group. Conclusion : In this study, renal defects were found in quite high percentages; 28 percent patients without reflux and 38 percent patients with low grade VUR, respectively. Moreover, there was no significant difference in the incidence of renal defects between the low grade and moderate grade groups. Therefore, DMSA scan should be performed for infants with UTI as a screening test regardless of the presence of VUR. 목 적 : DMSA 신 스캔은 신장에 대한 높은 방사능의 위험성과 낮은 grade의 역류시 불필요한 것으로 인식되어 최근 연구들에서는 낮은 방광요관역류가 있을 시에는 조기검사에 포함시키지 않는 것으로 보고되고 있다. 그러나 실제 임상에서는 낮은 grade의 역류 환아에서도 신피질결손이 보이는 경우가 있어 DMSA 신 스캔의 조기검사로서의 필요성에 대해 재조명하여 보고자 하였다. 방 법 : 1995년부터 2004년까지 상계백병원 소아과에서 요로감염으로 진단된 환아들 중 신장초음파, DMSA 신 스캔, 배뇨성 방광 요도 조영술을 모두 시행한 189명의 환아를 대상으로 하였다. 검사들은 요로감염으로 진단된 지 적어도 1개월 이내에 시행하였다. 방광요관역류의 정도는 grade I-II는 경도(low grade), grade III은 중등도(moderate grade), grade IV-V는 중증(high grade)으로 각각 분류하였다.결 과 : DMSA 신 스캔상 신피질결손은 총 189명 중 67명, 378 신단위 중 82 신단위에서 보였다. 신피질결손의 빈도는 역류가 없을 때 28%, 경도일 때 38%, 중등도일 때 53%, 중증일 때 100%로 역류가 심할수록 신피질결손의 빈도도 높아지는 것으로 나타났다(P<0.01). 중등도일 때의 신피질결손의 빈도는 경도일 때의 신피질결손의 빈도와 의미있는 차이는 없었다(P> 0.05).

빅벨리해마(Hippocampus abdominalis) 글루코코르티코이드 수용체의 분자 유전학적 동정과 발현 분석

고지연,오민영,이숙경,완창,이제희,S.D.N.K. Bathige 한국수산과학회 2015 한국수산과학회지 Vol.48 No.3

Glucocorticoids (GCs) are steroid hormones regulated through responses to stress to maintain diverse metabolic and homeostatic functions. GCs act on the glucocorticoid receptor (GR), a member of the nuclear receptor family. This study identified and characterized the GR gene from the big-belly seahorse Hippocampus abdominalis designating it HaGR. The open reading frame of the HaGR cDNA was 2,346 bp in length, encoding a 782-amino-acid polypeptide with a theoretical isoelectric point of 6.26 and predicted molecular mass of 86.8 kDa. Nuclear receptors share a common structural organization, comprising an N-terminal transactivation domain, DNA-binding domain, and C-terminal ligand-binding domain. The tissue-specific mRNA expression profile of HaGR was analyzed in healthy seahorses using a qPCR technique. HaGR mRNA was expressed ubiquitously in all of the tissues examined, with the highest expression levels in kidney, intestine, stomach, and gill tissues. The mRNA expression in response to immune challenge with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), Edwardsiella tarda, and Streptococcus iniae revealed that it is inducible in response to pathogen infection. These results suggest that HaGR is involved in the immune response of the big-belly seahorse.

이성천의 <대지의 노래> 분석

고지연 민족음악연구회 2001 민족음악의 이해 Vol.- No.9

단간 내도복 메조 ‘단아메’

고지연,송석보,최명은,곽도연,김정인,박장환,최지명,우관식,정태욱 한국육종학회 2017 한국육종학회지 Vol.49 No.4

‘Daname’ is a foxtail millet(Setaria italica Beauv.) variety developed and registered by Department of Southern Area Crop Science, NICS, RDA in 2014. This variety which collected from IT252182 was developed through pure line selection. ‘Daname’ is a early-maturing variety having 100 days of growth period from seeding to harvesting in Milyang of Korea. The culm length is about 97 cm which is 20cm shorter culm length than standard variety ‘Hwanggeumjo’, and it showed tolerant to lodging in field. The panicle shape is a cylindrical and the length is about 19cm. According to the grain, seed color and dehusked grain colors are orange and yellow and endosperm characteristic is non-glutinous. The yield performance of this variety was about 3.81 t ha-1 in local adaptability test from 2013 to 2014 and it showed similar with standard variety ‘Hwanggeumjo’. As the addition amount of ‘Daname’ in rice increased from 0% to 30% and 100%, the antioxidant ability of ‘Daname’ added rice and hardness/stickiness balances were increased. But we could not find out the difference of hardness/stickiness balances between 0%and 10% addition. So, 10% addition amount was concluded to increase antioxidant ability regardless eating quality of cooked rice. (Variety registration No. 6243)

빅벨리해마(Hippocampus abdominalis)에서의 Mitochondrial Heat Shock Protein 75 유전자의 특징과 발현 분석

고지연,완창,이숙경,S.D.N.K. Bathige,오민영,이제희 한국수산과학회 2015 한국수산과학회지 Vol.48 No.3

Mitochondrial heat shock protein 75 (mtHSP75) is a member of the HSP90 family and plays essential roles in refolding proteins of the mitochondrial matrix. Mitochondria provide energy in the form of ATP and generate reactive oxygen species (ROS). Heat shock proteins (HSPs) are activated in response to stress, and protect cells. In this study, we characterized the mtHSP75 of the big-belly seahorse Hippocampus abdominalis. The protein (BsmtHSP75) is encoded by an open reading frame (ORF) of 2,157 nucleotides, has 719 amino acids (aa), and is of molecular mass 82 kDa. BsmtHSP75 has two functional domains, a histidine kinase-like ATPase (HATPase_c) domain (123–276 aa) and an HSP90 family domain (302–718 aa). BsmtHSP75 was expressed in all tested tissues of healthy seahorses. The ovary contained the highest transcription level, followed (in order) by the blood, brain, and muscle. Pouch tissue showed the lowest expression level. The expression of BsmtHSP75 was significantly (P<0.05) up-regulated on viral or bacterial challenge, suggesting that BsmtHSP75 plays a role in the immune defense against bacterial and viral pathogens. Mitochondrial heat shock protein 75 (mtHSP75) is a member of the HSP90 family and plays essential roles in refolding proteins of the mitochondrial matrix. Mitochondria provide energy in the form of ATP and generate reactive oxygen species (ROS). Heat shock proteins (HSPs) are activated in response to stress, and protect cells. In this study, we characterized the mtHSP75 of the big-belly seahorse Hippocampus abdominalis. The protein (BsmtHSP75) is encoded by an open reading frame (ORF) of 2,157 nucleotides, has 719 amino acids (aa), and is of molecular mass 82 kDa. BsmtHSP75 has two functional domains, a histidine kinase-like ATPase (HATPase_c) domain (123–276 aa) and an HSP90 family domain (302–718 aa). BsmtHSP75 was expressed in all tested tissues of healthy seahorses. The ovary contained the highest transcription level, followed (in order) by the blood, brain, and muscle. Pouch tissue showed the lowest expression level. The expression of BsmtHSP75 was significantly (P<0.05) up-regulated on viral or bacterial challenge, suggesting that BsmtHSP75 plays a role in the immune defense against bacterial and viral pathogens.

조(Setaria italica Beauvios) 품종과 첨가비율에 따른 오메기떡의 이화학적 특성

고지연,우관식,이재생,송석보,김정인,정미선,윤동경,김기영,정태욱,오인석 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

조 배유 특성별 분말 즉석 죽의 품질 특성 및 항산화 활성

고지연,송석보,최명은,우관식,최지명,곽도연,김기영,정태욱,고종철,오인석,Ko, Jee Yeon,Song, Seuk Bo,Choe, Meyong Eun,Woo, Koan Sik,Choi, Ji Myeong,Kwak, Do Yeon,Kim, Ki Yong,Jung, Tae Wook,Ko, Jong Cheol,Oh, In Seok 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.4

This study was conducted in order to investigate the antioxidant capabilities and quality characteristics of instant porridge made of foxtail millet (Setaria italica Beauv.) from different varieties with different endosperm characteristics and processing using different methods. The two foxtail millet varieties used in this study were waxy foxtail millet, 'Samdachal', and non-waxy foxtail millet, 'Samdame'. The means of processing food to create instant porridge were dry cereal frying, wet cereal frying, freeze drying after steaming, popping, and no treatment. The WAI (water absorption index) of instant porridge made of waxy foxtail millet was higher than that of non-waxy foxtail millet, and popping created the highest WAI among the treatments tested. Whean we analyzed RVA characteristics of non-waxy foxtail millet instant porridge, peak viscosity and setback values were decreased in all processed foxtail millet instant porridge compared to non-waxy foxtail millet instant porridge. In waxy foxtail instant porridge, the peak and final viscosities of processed treated instant porridge increased. The viscosity just after pouring hot water into the instant porridge was higher in waxy foxtail millet porridge treated by freeze drying after steaming, popping, and wet cereal frying; the viscosity of non-waxy foxtail porridge was increased after popping as compared with the other treatments. The polyphenol contents and antioxidant activities were increased in all processed, treated-instant porridge except for freeze dried porridge after steamingnt. The popping treatment showed the highest polyphenol contents (1.5 fold), and ABTS antioxidant activity (1.2 fold), compared to no treatment. Non-waxy foxtail millet porridge produced by popping earned the highest sensory evaluation scores.

경도의 방광요관역류가 있는 소아에서 ^99mTc-dimercaptosuccinic acid 신 스캔의 필요성

고지연,구자욱,Koh, Ji Yeon,Koo, Ja Wook 대한소아청소년과학회 2006 Clinical and Experimental Pediatrics (CEP) Vol.49 No.6

Objective : $^{99m}Tc$-dimercaptosuccinic acid(DMSA) scan is considered to be the most sensitive examination for detection of renal scars. However, because of its high radiation exposure to the kidney and its limited usefulness for patients with low grade vesicoureteral reflux(VUR), some authors have suggested that DMSA scans should be reserved primarily for children with VUR grade 3 and above. The aim of this study was to reevaluate the necessity of DMSA scans as a screening test in infants without reflux or with low grade reflux. Methods : In this retrospective study, 189 infants(mean age : 6.2 months) diagnosed as UTI were enrolled. Voiding cystourethrogram(VCUG), DMSA scan and renal ultrasonography were performed within 1 month of UTI. VUR grade was classified into three subgroups; low grade(grade 1-2), moderate grade(grade 3), and high grade(grade 4-5), respectively. Results : Renal defects were present in 67 of 189 infants, and 82 of the 378 renal units. The incidence of renal defects was significantly correlated with VUR grade(P<0.01); 28 percent without reflux, 38 percent with low grade, 53 percent with moderate grade, 100 percent with high grade, respectively. However, there was no significant differences in incidence of renal defects between the low grade and moderate grade group. Conclusion : In this study, renal defects were found in quite high percentages; 28 percent patients without reflux and 38 percent patients with low grade VUR, respectively. Moreover, there was no significant difference in the incidence of renal defects between the low grade and moderate grade groups. Therefore, DMSA scan should be performed for infants with UTI as a screening test regardless of the presence of VUR.