현장이야기 : 사모코너 ; 러브레터

안소연 기독교대한성결교회 활천사 2001 활천 Vol.569 No.-

Aspergillus niger 글루코오스 산화효소의 유도 발현

안소연,조현영,공광훈 中央大學校 基礎科學硏究所 2002 基礎科學硏究所 論文集 Vol.16 No.-

Glucose oxidase catalyzes the oxidation of β-D-glucose to gluconic acid. It is necessary to be obtained maximum glucose oxidase activity because the enzyme is used commercially for various applications, in particular extracelluar glucose oxidase. For high activity of extracellular glucose oxidase, we used the enzyme from Aspergillus niger and YEP medium. Extracellular glucose oxidase activity as high as 7.5Uml^-1 was obtained with sucrose as carbon source and peptone as nitrogen source. The result of testing a various of medium condition was obtainable the highest enzyme activity in condition of culture medium of 1% sucrose(w/v), 2% peptone(w/v) and 1% yeast extract(w/v) at 25℃. Our work indicate an economically attractive process for enzyme production. In addition Aspergillus niger glucose oxidase was purified by DEAE-Sephacel chromatography. The activity of the enzyme proceeded at pH 5.5 and the effective substrate of the enzyme was only glucose.

인체 글루타티온 전달효소에 있는 알지닌 13 변이체의 기질 특이성에 관한 연구

안소연,공광훈 中央大學校 基礎科學硏究所 2001 基礎科學硏究所 論文集 Vol.15 No.-

In order to study the roole of residue in the active site of glutathione S-transferase(GST), Arg13 residue in human GST P 1-1 was replaced with alanine, leucine or lysine by site-directed mutagenesis to obtain mutants R13A, R13L and R13K. These mutants were expressed in Escherichia coli and purified to electrophoretic homogeneity by affinity chromatography on immobilized GSH. The specific activities were determined by measuring the initial rates of the enzymes-catalyzed conjugation of GSH towards electrophilic substrates. Our results suggest that Arg13 in human GST P1-1 contributes to the binding of electroophilic substrate, but it is not needed for the glutathione peroxidase activity and the steroid isomerase activity of human glutathione S-transferase p1-1.

걸프 왕정 국가들의 기성 정치 질서에 도전하는 카타르 유인 분석-수정주의 국가 관점을 기반으로-

안소연 부산외국어대학교 지중해지역원 2023 지중해지역연구 Vol.25 No.1

In 2017, Saudi Arabia, Egypt, the United Arab Emirates and Bahrain declared that they would cut all ties with Qatar. Those countries accused Qatar of supporting Sunni Islamist groups such as the Muslim Brotherhood(MB), or Hamas, and the Iranian ambition to extend regional power in the Gulf region. For the Sunni monarchy countries, both the MB and Iran are regarded as threats to their legitimacy. While Qatar maintains good relations with Iran and Islamist groups, it also hosts the American air base. In fact, it is difficult to understand contradictory Qatar’s foreign policy. Therefore, this study examines why Qatar challenges the status quo of the Gulf regional order based on the perspective of the revisionist states’ motivations. This study investigates Qatar’s motivation to challenge the status of of the Gulf regional politics in terms of revisionist state theory. Thus, this study contributes to confirming that based on economic development, social and political stability, Qatar was motivated to seek to challenge the existing order in favor of Qatar’s political interest to extend its power and influence.

고등학교 통계동아리를 통한 통계 체험활동 개발 및 활성화 방안 탐색

안소연,배준하,박지현 한국수학교육학회 2016 수학교육 학술지 Vol.2016 No.3

Vancomycin-Associated Spontaneous Cutaneous Adverse Drug Reactions

안소연,황의경,김주희,김정은,진현정,진선민,견진옥,이영희,박해심,최영화,임승관,예영민 대한천식알레르기학회 2011 Allergy, Asthma & Immunology Research Vol.3 No.3

Purpose: With the increase in vancomycin use, adverse drug reactions (ADRs) associated with vancomycin have been reported increasingly more often. However, the characteristics of cutaneous ADRs with and without systemic reactions (SRs) have not been described. This study investigated the characteristics of spontaneously reported and assessed ADRs associated with vancomycin by a pharmacovigilance center. Methods: ADRs (n=121) associated with vancomycin in 96 patients were collected from 2008 to 2009. Records from physician- and nurse-reported suspected cases of vancomycin ADRs, ADR type, latent period, and laboratory results were compared between cutaneous ADRs with and without SRs. Results: The main vancomycin-related ADRs were skin rashes (47.9%), hematologic abnormalities (17.36%), fever (12.4%), and elevated serum creatinine (12.4%). Significant differences were observed in latent period (days) and the mean change in eosinophils (%) between cutaneous (9.21±9.71 and 1.4±3.4, respectively) and other ADRs (14.03±11.71 and -0.5±3.5, respectively). Twelve cases of cutaneous ADRs with SRs had been initially reported as cutaneous ADRs only. Mean changes in the eosinophil count were significantly higher for cutaneous ADRs with SRs compared to those without SRs. Conclusions: Skin rashes accompanied by peripheral eosinophilia, representing suspected immune-mediated delayed hypersensitivity reactions,are a common vancomycin ADR. For the early and exact detection of ADRs associated with vancomycin administration, close monitoring of laboratory tests, including complete blood counts with differential analysis, is recommended.

어린이의 치과치료시 약물에 의한 진정요법 사용에 대한 실태조사

안소연,최병재,곽지윤,강정완,이제호 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.3

진정요법은 소아치과에서 사용하는 보상, 속박, 체계적 탈감작법(말-시범-시행), 친밀감 같은 통상의 방법으로는 환자의 반응이 개선되지 않아 일반적인 치과치료가 불가능할 때 사용하는 행동조절법이다. 최근 국내에서도 진정요법을 이용하는 사례가 증가하는 추세이나 그 기준이나 방법들에 대한 연구는 매우 부족한 상태이다. 미국소아치과학회의 진정요법에 관한 기준이 있기는 하지만 국내의 상황이 미국과 다르므로 한국에서의 연구가 필요하다. 이에 저자는 국내에 거주하고 있는 소아치과의사들을 대상으로 국내 소아치과에서의 진정요법 현황에 대한 실태를 조사, 그 결과를 정리하여 향후 소아치과 임상에서의 진정요법에 대한 임상 기준을 정하는데 기여하고자 국내에 거주하고 있는 대한소아치과학회 회원 573명을 대상으로 진정요법 사용 실태에 관한 설문지를 발송하여 이 중 회신을 한 220명의 설문을 분석하여 다음의 결과를 얻었다. 1. 응답자의 약 66%가 진정요법을 사용하고 있다고 답했다. 진정요법에 관한 이전 연구 결과와 비교해 볼 때, 국내 소아치과에서 진정요법의 사용빈도가 증가하였다. 2. 진정요법으로 치료를 결정 하게 된 요인은 행동조절, 치료내용과 양, 보호자의 요구, 내원횟수, 전신질환의 순서이었다. 3. 진정요법으로 치료받는 환자의 연령은 만 3세가 가장 많았고, 만 4-5세, 만 2세 미만, 만 6-10세, 만 10세 이상의 순서로 조사되었다. 4. 진정요법 시 chloral hydrate는 60-70mg/kg, hydroxyzine은 10-40mg/kg(25mg/kg)을 사용하고 있었고, 경구 투여가 가장 선호하는 약물투여 경로였다. 5. 진정요법 시 사용하는 환자감시 방법은 피부나 손톱색 둥의 관찰을 포함한 환자 평가와 맥박 산소측정기(pulse oximeter)를 통한 환자감시를 선호하는 것으로 조사되었다. 6. 진정요법을 사용하고 있다고 응답한 사람의 약 56%에서 심폐소생술 교육을 받은 것으로 조사되었다. Sometimes the dentists encounter a child who can not be treated with traditional behavior management techniques (for example, reward, restraint, Tell-Show-Do, familialization). In such a case, the dentists use sedation technique. Recently, in Korea, the use of sedation by pediatric dentists is increased. But, the guideline and survey of sedation is very insufficient. Now, we need a survey of sedation practice in Korea. We carried out research on the actual condition about sedation with a questionaire to pediatric dentists in Korea. Followings are the conclusions 1. Sixty six percent of pediatric dentists use sedative agents in their practice. In this study, using sedation shows an increase as compared with the past. 2. Determinative factors of using sedation were orderly behavior management, number of visiting, guidian's opinion, amount of treatment, general condition. 3. Distribution of ages in patients sedated with agents was orderly 3 years, 4-5 years, under 2 years, 6-10 years, more than 10 years. 4. Particular sedative drugs were chloral hydrate 60-70mg/kg, hydroxyzine 10-40mg/kg(25mg/kg), and oral route was the most favorable route. 5. Observation of skin and nail color, pulse oximeter were the most frequently utillized monitoring method during sedation. 6. Only fifty six percent of pediatric dentists complete the cardiopulmonary resuscitation course.

대한소아치과학회 연차총회에서 발표된 구술 및 포스터 초록의 출판률 변화

안소연,정성우,김범수,손정완 (사)한국스마트미디어학회 2022 스마트미디어저널 Vol.11 No.10

Previous studies in various medical specialties have shown that fewer than 50% of abstracts presented at meetings are subsequently published, but only a few studies have been performed in pediatric dentistry. The purpose of this study was to investigate the rate of publication of articles based on abstracts presented at the Korean Academy of Pediatric Dentistry (K.A.D.P) spring and fall Congress for 2001 to 2011. The abstracts for both oral and poster presentation were collected. A RISS search was then performed to identify the publication of full-length articles based on those titles of the abstracts. A total of 706 abstract presentations were done at the 24 meetings (477 as oral presentation, 229 as poster presentations). Of these, from 45.2%(319) in 2011 to 82.9%(585) in 2022 was subsequently published. The publication ratio for orally presented abstracts was from 52.2%(249) in 2011 to 86.6%(413) in 2022, poster presentations from 30.6%(70) in 2011 to 75.1%(172) in 2022. We suggest that presenters at these meetings should expand their abstracts into full manuscripts and seek to publish them in peer-reviewed journals for the benefit of the profession. We believe that the results of changes in the publication rate over the past 12 years are attributable to the digitalized environment such as electronic literature search and electronic publication. 의학 전공분야의 학회의 초록이 출판으로 이어지는 비율은 50% 이하라는 이전의 연구들이 있었지만, 소아치과 분야에서는 이에 대한 연구가 거의 없었다. 이번 연구의 목적은 2001년에서 2011년까지의 개최된 대한소아치과 춘추계 학술대회의 초록들 중 어느 정도가 논문으로 출판되는지에 관한 것이다. 구연과 포스터를 위한 초록들을 모두 조사했다. 이 초록들의 제목을 이용해서 학술연구정보서비스에서 논문들을 검색하였다. 총 24번의 학회에서 706개의 초록 (477개의 구연, 229개의 포스터)가 있었고, 이 중 2011년 45.2%(319)에서 2022년 82.9%(585)가 논문으로 출판되었다. 구연은 52.2%(249)에서 86.6%(413), 포스터는 30.6%(70)에서 75.1%(172)의 출판율을 보였다. 우리는 최근 12년 간의 출판율 변화 결과가 전자 문헌검색과 전자 출판 등 디지털화 된 환경에서 기인한다고 생각한다.

3D 컴퓨터 드로잉 프로그램을 활용한 도형학습 프로그램이 수학학습부진학생의 공간능력에 미치는 영향 방안

안소연,강옥려 한국학습장애학회 2016 학습장애연구 Vol.13 No.2

This study was to investigate how the geometry learning program with three-dimensional drawing program impacts on students with/out mathematical learning difficulties for their spatial visualization ability and spatial orientation ability. The subjects were two classes of the sixth grade - six students with mathematical learning difficulties and forty students without learning difficulties. The experimental group had geometry learning program with three-dimensional drawing program. The control group had a regular geometry learning class on the basis of regular curriculum. This study used pretest and posttest control group design. Thee covariate analysis was used to figure out degree of changes in spatial ability. The spatial ability of students with mathematical learning difficulties between two groups was also compared. Research findings were as follows. First, students without mathematical learning difficulties in the experimental group showed the improvement degree of spatial visualization ability in comparison with their counterpart in the control group. However, there was no statically significant difference. Second, students with mathematical learning difficulties in the experimental group demonstrated improvement in spatial visualization ability than their counterpart in the control group. Those in the experimental group showed increased degree of spatial orientation ability as well, compared to the others in the control group. As a result, the geometry learning program using three-dimensional drawing program has positive impact both on students with/out mathematical learning difficulties for their overall spatial capability and spatial visualization ability. Some discussion and suggestions were offered. 본 연구는 3D 컴퓨터 드로잉 프로그램을 활용한 도형수업이 수학학습부진학생과 일반학생의 공간능력과 하위요소인 공간시각화 능력과 공간방향화 능력에 어떠한 영향을 미치는지 살펴보았다. 연구 대상자는 초등학교 6학년 수학학습부진학생 6명과 그들이 속해있는 두 학급 일반학생 40명이었다. 실험집단은 3D 컴퓨터 드로잉 프로그램을 활용한 도형학습 프로그램을, 통제집단은 교육과정에 기초한 일반적인 도형수업을 실시하였다. 본 연구는 사전-사후검사 통제집단 설계를 사용하였으며, 공간능력과 하위요소의 두 집단 간 변화 정도를 알아보기 위해 공변량분석을 사용하였고, 수학학습부진학생의 공간능력과 하위요소의 변화 정도를 확인하였다. 연구의 결과, 첫째, 일반학생의 경우 전체적인 공간능력과 하위요소인 공간시각화 능력에서는 3D 컴퓨터 드로잉 프로그램을 활용한 도형학습 프로그램에 참여한 실험집단과 참여하지 않은 통제집단 사이에 유의한 차이가 있는 것으로 나타났다. 그러나 하위요인인 공간방향화 능력은 평균적인 점수는 향상하였으나 통계적으로는 유의한 차이가 나타나지 않았다. 둘째, 실험집단과 통제집단의 수학학습부진학생의 변화 정도를 비교하여 확인한 결과 실험집단의 전체적인 공간능력과 하위요소인 공간시각화 능력은 통제집단에 비해 향상도가 매우 높았다. 실험집단의 공간방향화 능력 점수 향상도는 통제집단에 비해 약간 높은 모습을 보였다. 본 연구는 3D 컴퓨터 드로잉 프로그램을 활용한 공간능력 향상 프로그램을 일반학생뿐만 아니라 수학학습부진학생에게 적용하여 효과를 입증하였다는 점에서 의의를 가진다. 연구 결과를 토대로 논의와 앞으로의 연구를 위한 제언을 하였다.

약인성 간손상 환자에 대한 生肝湯 치험 1례

안소연,주성희,허소영,장은경,이장훈,김영철 대한한방내과학회 2019 大韓韓方內科學會誌 Vol.40 No.2

Objectives: The purpose of this case study is to report the effect of Korean Medicine treatment on a patient diagnosed with drug-induced liver injury by oral administration of acetaminophen. Methods: A 21-year-old male patient with drug-induced liver injury visited the clinic five times from January 28th, 2019 to March 16th, 2019. The patient took Saenggan-tang extract during the treatment period. Results: The levels of serum alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) were significantly decreased. The chief complaints of the patient, such as yellow-red urine and fatigue, were also relieved. Conclusion: Traditional Korean medicine has a beneficial effect on drug-induced liver injury.