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면역효소법에 의한 한국산 쑥 화분에 대한 특이 IgE 측정 및 Radioallergosorbent Test ( RAST ) 와의 상관성에 관한 연구
박해심(Hae Sim Park),홍천수(Chein Soo Hong),이수곤(Soo Kon Lee),오승헌(Seung Hun Oh) 대한내과학회 1988 대한내과학회지 Vol.34 No.2
N/A We developed a micro-KLISA assay by use of Korean sagebrush allergen to measure sagebrush-specific IgE. The assay was carried out in polyethylene flat microtitre plates by incubating the allergen, 10% new-born calf serum, test sera and finally horseradish peroxidase conjugated monoclonal anti-IgE. Preincubated with 1 mg/ml of the allergen we prepared, W5-RAST was inhibited by 80.9% and the inhibition curve showed dose-responsive pattern with serial dilution of crude lyophilized sagebrush extracts. The cut-off point for this assay for specific IgE was established by that mean and S.D values from 30 different negative control sera were 0,012 and 0,014 in absorbance value, respectively (Mean+ 2S.D. =0.041). The positive probability of ELISA and RAST assay was 76% and 74%, respectively in 52 patients with positive skin prick test with korean sagebrush extract. The influence from high levels of total IgE was negligible, ranged from 0.063 (5000 u/ml of IgE) to 0.082 (1000 u/ml of IgE). The coefficient variation for the intrassay and interassay reproducibility ranged from 2.83 to 24.14% and 9.64 to 27.03%, respectively. The absorbance value obtained in this assay correlated with the bound radioactivity (%) in RA5T assay (r= 0.553, p<0.05), This showed a higher degree of correlation (r=0.41) with the wheal size on skin prick test than that. This ELISA assay is well suited for mass screening and can be a useful method for measuring specific IgE. It compares favorable in sensitivity and specificity with RAST assay.
환삼덩굴 화분증 : 천식유발시험상 확진된 환자들의 임상적 특징
박해심,최소연,남동호,김희연 (Hae Sim Park,So Yeon Coi,Dong Ho Nahm,Hee Yeon Kim) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.1
Baekground: Hop Japanese (Hop J) pollens are abundant in the air of Korea during the autumn season. Their significances as a source of allergenic sensitization have been underestimated in this country. Msterial and Method; In other to observe clinical features of Hop J-sensitive asthmatic patients in this country, skin prick test with Hop J pollen was performed. The serum specific IgE antibodies to Hop J pollen antigen were detected by enzyme linked immunosorbent assay (ELISA) in positive responders (>2+ of A/H ratio) on skin prick test. To confirm the respiratory sensitization, bronchoprovocation test was performed in 17 asthmatic patients sensi- tive to this pollen. Result: Ten asthmatic subjects showed a significant bronchoconstriction following the inhalation of Hop J pollen extract (6 early and 4 dual astmatic responses) and all of them had high serum specific IgE bindings, with minimal bindings in negative responders. They have suffered from seasonal aggravation of asthmatic symptoms with or without rhinitis, and/or conjunctivitis symptoms. The skin reactivity to Hop J had more than 5+ of A/H ratio on skin prick test in nine positive responders, whlie negative responders showed from 1+ to 3+ response. Moreover, four (40%) asthmatic subjects showed a positive response to only the Hop J pollen on skin prick test and an isolated positive asthmatic response to the Hop J bronchoprovocation test. Conelusion'. We believe that the Hop J pollen should be considered as an allergen during the Autumn season, and thus included in skin test batteries in this area. Some labelled having intrinsic asthma or rhinitis might be sensitized to this pollen or other unknown allergens.