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박종흠,김재경,송범석 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.11
Mistletoe offers health-promoting effects; however,it has toxicity, requiring careful application. Viscothioninis a polypeptide of mistletoe that whilecontributing to toxicity also demonstrates anti-cancer andanti-diabetic activities. The aim of this study was to evaluatewhether gamma irradiation or heating treatment couldselectively reduce viscothionin-mediated cytotoxicity. Gamma irradiation effectively inhibited viscothionin-inducedcytotoxicity to RIN5mF cells, but heating treatmentdid not affect its cytotoxicity. Both heating and gammairradiation further increased the insulinotropic activity ofviscothionin, whereas the effect of gamma irradiation wasdose-dependent and diminished above 20 kGy. Structuralanalysis showed that gamma irradiation significantlyaltered the ordered structure of viscothionin, unlike heatingtreatment, resulting in a change of its molecular properties,which could be linked to the observed changes in thecytotoxicity and insulinotropic activity of the polypeptide. These results suggest gamma irradiation as an alternativemethod for minimizing viscothionin toxicity withoutinterfering with anti-diabetic effect.
감마선처리 냉동 우육, 분쇄우육 및 떡갈비의 판별을 위한 Comet assay의 적용성 평가
박종흠,김재경,송범석 (사)한국방사선산업학회 2023 방사선산업학회지 Vol.17 No.3
This study was conducted to evaluate the applicability of the Comet assay, which is widelyused for the identification of irradiated meats, to detect irradiated beef cut, ground beef, and Tteokgalbiduring freezing storage. Gamma-irradiation significantly increased the DNA damage in frozen beef cutand ground beef samples. Among those, DNA nuclei of samples irradiated with absorbed doses of 1 kGyor more showed typical comet-shaped damage, convincing that the samples were irradiated. Meanwhile,DNA nuclei in non-irradiated beef cut and ground beef samples were also damaged according to storagetime. In particular, since the damage of DNA nuclei in the non-irradiated samples frozen for threemonths was similar to that of samples irradiated with a dose of 0.5 kGy, it was considered difficult todetect whether these samples were irradiated by Comet assay analysis. Likewise, gamma-irradiationof Tteokgalbi increased DNA damage. However, significant damage to DNA nuclei was observed even inthe non-irradiated samples. Therefore, the application of the analysis method to determine whether theTteokgalbi sample was irradiated was not appropriate. In conclusion, these results suggest that Cometassay could be limitedly applied only to fresh meat with a short storage period and minimal processing.
감마선과 전자선 조사처리된 들깨와 대두의 신속 판별을 위한 유전자 코메트 분석
박종흠,김재경,박하영,이윤종,김재호,연영흠,송범석 (사)한국방사선산업학회 2019 방사선산업학회지 Vol.13 No.2
This study was carried out to investigate the applicability of comet assay, which isapproved as a formal detection method only for the identification of irradiated fresh meats inKorea, to identify irradiated perilla seeds and soybeans at doses at 0, 0.5, 1, 2, and 3 kGy withgamma rays or electron-beams (e-beams). The florescent images for comet assay were obtained bymicroscopic observation and olive tail moment (OTM) of nuclei in a samples was measured by usinga comet analysis software. Irradiation with both gamma rays and e-beams increased the ratio ofconsiderably damaged nuclei (OTM≥30) in both perilla seeds and soybeans as the absorbed doseincreased. Moreover, the average OTM exhibited close correlations with the absorbed dose in bothfood models, regardless of radiation sources (R2 of regression curves: 0.91~0.98). On the otherhand, all samples irradiated at more than 1 kGy could be discriminated from non-irradiated oneby visual inspection of typical comets of nuclei generated by irradiation using fluorescent microscopewithout the comet analysis software. Based on these results, it is considered that the cometassay can be used as a rapid identification method for irradiated perilla seeds and soybeans withgamma rays and e-beams, because the evaluation for irradiation using this method can be drawnwithin 3 hours.
천왕보심단(天王補心丹)의 항우울효과 및 monoamine 대사에 미치는 영향
박종흠,배창욱,전현숙,홍성유,박선동,Park Jong-Heum,Bae Chang-wook,Jun Hyun-Suk,Hong Sung-You,Park Sun-Dong 대한한의학방제학회 2004 大韓韓醫學方劑學會誌 Vol.12 No.2
Depression is a sort of mental disorder which is very common. To treat depression, many drugs such as TCA, MAOI are developed and used. But they have a lot of side effects, so it needs to develop drugs without side effects or with less side effects. Herbal medicines have been used to treat diseases not only physical but also mental and have less side effects. therefore, it has been thoght the need to develop herbal medicine with antidepressant effect. The purpose of this study was to reseach antidepressant effect and influence on monoamines of chunwangboshimdan thought to have antidepressant according to ancient medical book- donguibogam- and recent reports. We used 'forced swimming test(FST)' to know antidepressant effect of chunwangboshimdan and HPLC to check the influence on monoamines and their metabolites(norepinephrine, dopamine, DOPAC, HVA, serotonin, 5-HIAA) of chunwangboshimdan after divided into cerebral cortex, striatum, hypothalamus and hippocampus. The results were obtained as follows: In the study of antidepressant effect by 'forced swimming test(FST)'method, chunwang boshimdan had a significant antidepressant effect. In the study of influence on monoamines by HPLC, chunwangboshimdan mainly increased dopamine among monoamines and their metabolites(norepinephrine, dopamine, DOPAC, HVA, serotonin, 5-HIAA) significantly in 4 parts of rat's brain above-mentioned. Calculated by turnover ratio formulae of monoamine, chunwangboshimdan has more results than Imipramine. These results suggest that chunwangboshimdan has antidepressant effect that is related with the increase of monoamines by suppressing their metabolism as its mechanism.
최적회로 연결선 구조를 위한 설계 및 해석도구 (DATOIS)
박종흠,김준희,김석윤 대한전자공학회 1998 電子工學會論文誌, C Vol.c35 No.7
As the packing density of ICs in recent submicron IC design increases, interconnects gain importance. Because interconnects directly affect on two major components of circuit performance, power dissipation and operating speed, circuit engineers are concerned with the optimal design of interconnects and the aid tool to design them. When circuit models of interconnects are given (including geometry and material information), the analysis process for the given structure is not an easy task, but conversely, it is much more difficult to design an interconnect structure with given circuit characteristics. This paper focuses on the latter process that has not been foucsed on much till now due to the complexity of the problem, and prsents a design aid tool(DATOIS) to synthesize interconnects. this tool stroes the circuit performance parameters for normalized interconnect geometries, and has two oeprational modes:analysis mode and synthesis mode. In the analysis mode, circuit performance parameters are obtained by searching the internal database for a given geometry and interpolates results if necessary . In thesynthesis mode, when a given circuit performance parameter satisfies a set of geometry condition in the database, those geometry structures are printed out.
박종흠,성낙윤,변의백,송두섭,송범석,김재경,이주운,박상현,김재훈,강근옥,유영춘 한국방사선산업학회 2012 방사선산업학회지 Vol.6 No.3
The purpose of this study is to reduce the immune toxicity of lipopolysaccharide (LPS)by gamma irradiation. LPS was gamma-irradiated at the various doses of 20, 100 and 200 kGyand then evaluated on the immune toxicity through the cell proliferation, nitricoxide productionand cytokine release. Cell proliferation significantly decreased in the intact LPS treated groups,whereas gamma-irradiated LPS treated group were not reduced the cell proliferation. Similarly,the production of nitric oxide and cytokine showed the high levels in the intact LPS treated group. However, gamma-irradiated LPS treated group remarkably decreased the production of nitricoxide and cytokine in dose-dependent manner. Therefore, gamma irradiation may effective methodto reduce the immune toxicity of LPS.
전자선 조사가 베타글루칸의 항알레르기 활성에 미치는 영향
박종흠,성낙윤,변의백,송두섭,김재경,송범석,박상현,신미혜,이주운,김재훈,유영춘 한국방사선산업학회 2012 방사선산업학회지 Vol.6 No.3
This study evaluated the change in anti-allergy activity of β-glucan by electron beamirradiation. β-Glucan was irradiated at dose of 50 kGy and then orally pre-treated with electronbeam irradiated and non irradiated β-Glucan for 7 days. After pre-treatment, allergy was inducedby injection of ovalbumin (OVA). Serum total immunoglobulin E (IgE) and OVA-specific IgE levelsin the allergic mice was significantly increased but the mice pre-treated 50 kGy electron beam irradiatedβ-glucan was significantly decreased the levels of total IgE and OVA-specific IgE, respectively. Moreover, cytokine production (interleukin-4) was also decreased in the 50 kGy electronbeam irradiated β-Glucan pre-treated mice. These results indicate that pre-treatment of 50 kGyelectron beam irradiated β-glucan may elevate the anti-allergy activity. Therefore, electron beamirradiatedβ-glucan could be used for nutraceutical foods in food industry.