홍삼 사포닌이 Aspergillus flavus 의 발육과 Aflatoxin 생산에 미치는 효과

박재림,박효진,송동숙 한국균학회 1985 韓國菌學會誌 Vol.13 No.3

The study was carried out to find effects of the saponins that were extracted from red ginseng on the growth of, aflatoxins production by, and protein patterns of Aspergillus flavus NRRL 3357. A. flavus with 10^6 conidia at grown at 30℃ for seven days on the enriched medium. Mycelial growth and pH changes of medium which cultured the mold, were similar to those of the control group. However, aflatoxin which produced by the mold was less than that of the control in all concentration of the saponin. To be more specific, 0.3% of the saponin inhibited production of aflatoxin B₁ and G₁ to the extent of 31.6 and 21% of the control. The protein peaks of A. flavus at the fourth day of the culture were shown high intensity near the level of 14,300 daltons. However, the mold which cultured in the medium containing the saponin showed low intensity of protein than that of the control group on all molecular weight.

미생물 증식에 미치는 Soft silicate의 영향

박재림,박중철 신라대학교 자연과학연구소 2003 自然科學論文集 Vol.12 No.-

한국 Refresh 연구회로부터 제공받은 Soft silicate를 이용하여 미생물의 증식을 측정한 결과 배지첨가농도 0.5%로 대장균의 증식이 대조군의 약 3배 이상 촉진되었으며, L. casei는 대장균과 달리 0.1% 및 0.5%에서는 증식이 거의 관찰되지 않았으나 1% 및 3%첨가에서는 간헐적으로 대조군을 초과하는 증식이 관찰되었다. S. aureus는 시료 첨가농도 0.1%에서 최대증식을 나타냈고, 0.5%, 1% 순으로 각각 4배, 2배의 촉진효과가 나타났으며, V. parahaemolyticus는 첨가농도 0.1%가 약 3배, 0.5% 및 1%가 약 2배의 증식을 촉진시키는 추세를 나타냈지만, C. utilis 및 S. cerevisiae의 증식은 시료첨가 전 농도에서 증식정도가 대조군에 미치지 못하였다. The effect of soft silicate offered from Refresh Research Korea on growth of several microorganisms were tested. Growth of E. tali was stimulated more than 3 times of the control group when 0.5% soft silicate added in the broth. Growth of L. casei was not observed at the concentration of 0.1% and 0.5%. However, the growth of the organism was observed more than that of the control when 1% and 3% of the soft silicate added in the broth. Great peak showed at the concentration of 0.1% added in S. aureus and V. parahaemolyticus. Growth of C. utilis and S. cerevisiae were not reached to the growth of control group in all concentration added.

Saponin이 Aspergillus parasiticus의 발육과 Aflatoxin생합성에 미치는 효과

박재림,임광식,이종근 한국미생물학회 1985 미생물학회지 Vol.23 No.4

Aspergillus parasiticus의 발육과 afla toxin생산에 미치는 생약 extract의 효과에 따라 선정된 생약 금은화, 우방자 및 인삼의 조 saponm이 군체의 발육과 afla toxin생산능력에 미치는 효과을 측정하기 위하여 강화배지에 $30^{\circ}C$ 9 일간 배양하였다. 백삼 saponin 첨가배지 만이 균체발율이 대조군 보다 좋았다. 그러나 홍삼 saponin 0.36% 첨가배지에서의 균체발육은 대조군의 62.3%로 나타났으나, aflatoxin 생산능력은 aflatoxin B, 이 대조군의 38.7%. aflatoxin G1 이 22.9%로 좋은 억제효과를 나타냈다. 우방자 sapollln은 홍삼 saponin보다 억세효과가 못하지만 홍삼 saponin과 같이 균체의 발육과 aflatoxin 생산을 모두 억제 하는 것으로 나타났다. 한편 extract 에서 나타났던 금은화의 aflatoxin 생산 억제효과는 본 saponin 실험에서는 나타나지 않았다. The research was carried out for the purpose of finding effects of gerbal saponins on aflatoxin synthesis by Aspergillus parasitics NRRL 2999. A. parasiticus with $10^6$ conidia were grown at $30^{\circ}C$ for 9 days on the enriched medium that is optimum for the frowth and aflatoxins production by the mold. The inhibitory effect on the growth and aflatoxins produced by the mold occurred in the presence of 0.36% of crude red-ginseng saponin showing both the growth and aflatoxins production come to 62.3% (growth), 38.7% (aflatoxin $B_1$) and 22.9% (aflatoxin $G_1$) of the control. Thd next effective saponin to inhibit the growth and aflatoxins production was from burdock seeds. However, saponin extracted from honeysuckle flowers had no inhibitory effect. The mold caused no changes in the pH of the medium when it contained red-ginseng saponin. Red-ginseng saponin was more effective than the white-ginseng in inhibiting both the growth and aflatoxin production.

노령자의 건강관리에 관한 연구 -서울 지역을 중심으로-

박재림,고진복,오형근,김인택 한국환경보건학회 1981 한국환경보건학회지 Vol.7 No.2

This study aims to research on the actual state of the ways that the aged people live in Seoul use intentionally or unintentionally to maintain in their health. The aged people selected randomly for the study are 541 men and 902 women who are all more than 65 years old. This survey carried out from April to May in 1980. The results of this study are summed up as follows: 1. The main means of making a living engage was agriculture with the per cent of 57. 3 and next was commerce (11.9%). 2. As for their staple foods, the majority of them eat mixed food of rice and barley, and the rest eat rice or minor-grain mixed rice. As subsidiary foods, vegetables are the most common side dishes (68.9%) and see-weeds (6.7%) are taken by the minority of them. 3. Most of them (74.6%) frequently or occasionally take special health foods to maintaing their health. The most common health food turned out to be tonic liquor to men, while honey to women. 4. The majority of them(23.2% of men and 45.5% women) refrain from drinking and smoking. 5. 39.5 per cent of men and 25.5 per cent of wome) take light exercise such as going up hill and walking. 6. The majority of them undergo routine health check with the per cent of 10.8 or occasioanal health check with the 42.9. As medical treatment, many of them(45.9%) take Chinese remedies, western remedies(39.1%) and folk remedies (15.0%). 7. Many of them (66.0%) feel subjective symptoms about some geriatric diseases such as neuralgia, hypertension and cough. 8. Many of them (63.2%) free from mental trouble, while 17.7 per cent of them appeal to no job to do, 10.0 per cent of alienation and 9.1 per cent of domestic troubles. 9. Their subjective judgements of their secrets of health and longevity attach more importance to leading a regular life. 10. Most of them want to be served appropriate medical care, rest place, health consultations, etc. All these results reveal that health and longevity of the aged people are maintained by necessary cares and efforts, not by accident nor by apathy. It is thus highly desired tha tmany studies, enlightments and nationwide support about this problem be carried out in future.

수직 및 수평 단열판에 부착된 등온 사각비임에서의 자연대류 열전달

박재림,권순석 대한기계학회 1992 대한기계학회논문집 Vol.16 No.1

Laminar natural convection heat transfer from an isothermal rectangular beam attached to horizontal and vertical adiabatic plates has been studied for various aspect ratios of the beam and Grashof numbers. The local Nusselt number was increased with decreasing H/B for the constant beam width, B, and with increasing H/B for the constant beam height, H. The total mean Nusselt number of the vertical type was higher than that of horizontal type in the range of H/B.leq.0.52, but reversed in the H/B>0.52 at constant beam width. The total mean Nusselt number of the horizontal type was generally higher than that of vertical type at constant beam height. The total mean Nusselt number of the vertical type was higher than that of horizontal type in the range of H/B.leq.0.43 at constant wetted perimeter, but reversed in the H/B$\leq$0.43. 본 연구에서는 주위유체가 공기인 정상 층류 상태하에서 수직 및 수평단열판 에 부착된 등온 사각비임에서의 자연대류열전달 현상을 비임의 현상비와 Grashof수를 변수로 하여 고찰 하였다.

집단급식 관리에 관한 연구(Ⅲ) : 영양사에 대한 기초조사

박재림 대한영양사회 1980 國民營養 Vol.20 No.-

나? 무서운 하지감자여!

박재림 샘터사 2016 월간 샘터 Vol.555 No.-

주방공기중에서 분리된 포도상구균의 함생물질에 대한 감응성연구(1)

박재림 한국환경보건학회 1978 한국환경보건학회지 Vol.5 No.1

Antibiotics susceptibilities of Staphylococci were tested to get useful data for prevention food contamination and treatment of food poisoning. Tested were colony counts, isolation of Staphylococci, identification for the Staphylococcus aureus and resistant patterns to antimcrobial agents. The colonies and Staphylococci isolated from the atmosphere of 37 home kitchens in the morning (07:00-08:00) on Nov. 19, 1977 in Busan. The findings are as follows: 1. The average of colony counts was shown to be 9.6 ranging from 47 to 1 at mean temperature of 18$\circ$C (the highest 21$\circ$C, the lowest 15$\circ$C) 2. Out of total 37 kitchens, 18 Staphylococci were isolated from 15 kitchens with 40.5 per cent, and 4 Staphylococcus aureus were identified with 22.2 per cent from 18 Staphylococci. 3. Isolated Staphylococci were resistant to penicillin(100%), dihydrostreptomycin(93.3%), tetracycline (93.3%) and oleandomycin(66.6%), while the strains were sensitive to sulfonamide(88.6%) and colistin(83.3%). 4. Staphylococcus aureus identified were resistant to dihydrostreptomycin, penicillin and tetracycline, while the strains were sensitive to sulfonamide and colistin.

진균류의 증식과 대사에 미치는 방아(Agastache rugosa ) 추출물과 Estragole 의 효과

박재림,박송희,김정옥,김수원,이수영 한국식품위생안전성학회 1997 한국식품위생안전성학회지 Vol.12 No.1

The extracts from Agastache rugosa O. Kuntze, their chloroform and hexane fractions, and estragole identified from hexane fraction were tested to investigate the effects on the growth and metabolic activities of several true fungi. The fungi used were: Aspergillus oryzae KFCC 890, Aspergillus niger KCCM 11240, Saccharomyces cerevisiae IAM 459?, Saccharomyces ellipsoideus PNU 2215. The growth of S. cerevisiae by treatment of water extract (1%), hexane fraction (0.05%), and estragole (0.05%) were inhibited 93%, 50%, and 33% respectively, and S. ellipsoideus was also inhibited markedly with delaying the lag phase maximum 12 hrs. The growth of A. oryzae was inhibited by treatment of extracts and fractions. The ethanol production by S. cerevisiae was increased more than two times in the highest value around 42 hrs incubation by water extract, but chloroform fraction inhibited its production. The glucoamylase activities by A. niger were strongly inhibited by hexane and chloroform fractions (0.05%). The invertase activity by S. cerevisiae using estragole (0.05%) reached to 57.5% of control group. S. cerevisiae treated with the estragole was damaged the cell wall and cell membrane, leaked the protoplasm, and observed broken pieces of cell.

방아(Agastache rugosa O . Kuntze ) 로부터 동정된 estragole 과 방아추출물의 항균효과

박재림,김정옥,강혜윤,김운영,천화정 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.3

Water extract, and methanol extract, its chloroform and hexane fractions, and estragole from Agastache rugosa O. Kuntze were tested to find the inhibiting effect on the growth of several microorganisms. The organisms used were: Escherichia coli ATCC 1129, Staphylococcus aureus 1AM 1011, Vibrio parahaemolyticus WP, Bacillus aubtilis ATCC 6633, Aspergillus oryzae KFCC 890, Aspergidlus niger KCCM 11240. Water and methanol extracts at the concentration of 0.5%, and chloroform and hexane fractions at the concentration of 0.05% inhibited the growth of microorganisms from 1/5 to 2/3 of the control group. Eatregole identified from the hexane fraction as a major component, its authentic compound completely inhibited the growth of Vibrio parahaemolyticus completely at the concentration of 0.03%, and the other bacteria were at 0.05% .