인공재배 상황버섯 재배종 Phellinus linteus 와 P. baumii 자실체 추출물의 항산화활성과 폴리페놀 성분 비교

민경진,윤봉식,강희완 한국버섯학회 2020 한국버섯학회지 Vol.18 No.1

P. linteus(품종명: 고려상황, PLKS)와 P. baumii (장수 상황, PBJS)를 동일한 조건하에서 인공재배하여 얻어진 자실체를 70% 메탄올, 60% 에탄올, 열수추출하여 폴리페놀과 플라보노이드 측정 시 함량은 60%에탄올 추출물에서 가장 높게 나타났다. 60%에탄올을 용매로 PLKS와 PBJS의 자실체를 추출하여 농도별로 DPPH (2,2-diphenyl- 1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbisthiazoline- 6-sulfonic acid) 라디칼 소거능을 측정한 결과 PLKS와 PBJS는 거의 유사한 항산화 활성을 보였으며 자실체가 균사체보다 2-3배 이상 높은 항산화 활성이 측정되었으나 FRAP (ferric reducing antioxidant power)분석에서는 자실체와 균사체 간에 큰 차이가 없는 것을 확인하였다. PLKS와 PBJS의 자실체 ethyl acetate 분획물을 HPLC 분석한 결과 styrylpyrone 계 폴리페놀 성분인 davallialactone, hispidin, hypholomine B, inoscavin A를 검출할 수 있었으나 균사체에서는 검출되지 않아 자실체 특이적인 화합물인 것을 확인하였다. P. linteus (Korea Sanghwang, PLKS) and P. baumii (Jangsoo Sangwhang, PBJS) were artificially cultivated under the same conditions. Fruiting bodies were extracted using 70% methanol, 60% ethanol, and hot water. Phenol and flavonoid contents were optimally extracted using 60% ethanol. Antioxidant activities of 60% ethanol extracts from fruiting bodies and mycelia from each Sanghwang mushroom species were measured using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3- ethylbisthiazoline-6-sulfonic acid) radical scavenging activities, and FRAP (ferric reducing antioxidant power). The antioxidant activities of fruiting bodies were relatively higher in comparison to those of mycelial samples. In high performance liquid chromatography (HPLC) analysis, styrylpyrone-class poly phenolic compounds, davallialactone, hispidin, hypholomine B, and inoscavin A were detected in fruiting body samples of two Sanghwang mushroom species, but not in their mycelial samples.

Two cases of Chronic Intestinal Pseudo-obstruction treated by jejunostomy

민경진,이상훈 대한내과학회 2021 대한내과학회 추계학술대회 Vol.2021 No.1

A rare presentation of Rapunzel syndrome with multiple small bowel intussusceptions

민경진,차한,김성민,최재연 대한소아응급의학회 2019 대한소아응급의학회지 Vol.6 No.1

Rapunzel syndrome is caused by gastric trichobezoar with extended tail and small bowel obstruction. Patients with gastric trichobezoar can be asymptomatic until the bezoar increases in size. We report a case of a girl who visited the emergency department with abdominal pain. She was finally diagnosed with Rapunzel syndrome that causes multiple small bowel intussusceptions associated with trichophagia. Surgery was needed to reduce the multiple intussusceptions, and to remove the large trichobezoar. This case highlights to consider the possibility of Rapunzel syndrome when diagnosing the main cause of intussusceptions.

Carbofuran과 Chiorothalonil의 공존이 Brachydanio rerio(zebrafish)를 이용한 단기간 생물농축계수의 측정에 미치는 영향

민경진,차춘근 한국환경보건학회 1997 한국환경보건학회지 Vol.23 No.2

This study was performed to investigate the effect of co-existence of carbofuran and chlorothalonil on the short-term bioconcentration factor in Brachydanio rerio(zebrafish). The fishes were exposed to the single and combined treatment of carbofuran and chlorothalonil for 1, 3 and 5 days. Experimental concentrations of carbofuran were 0.05 and 0.10 ppm under the single treatment. And those of chlorothalonil were 0.005 and 0.010 ppm. Experimental concentrations of the combined treatment of carbofuran and chlorothalonil were 0.05 ppm+0.005 ppm, 0.05 ppm+0.010 ppm, 0.10 ppm+0.005 ppm for 1, 3 and 5 days, respectively. Carbofuran and chlorothalonil in fish and in test water were extracted with n-hexane and acetonitrile. GC-ECD was used to detect and quantitate carbofuran and chlorothalonil. 1-day, 3-day and 5-day bioconcentration factors(BCF$_1$, BCF$_3$ and BCF$_5$) of each pesticide were obtained from the quantitation results. The depuration rate of each pesticide was determined over the 24-h period after combined treatment. The results were as follows: Carbofuran did not bioaccumulate in zebrafish under the single and combined treatment for testing periods. BCF$_1$ values of chlorothalonil in concentration of 0.005 and 0.010 ppm under the single treatment were 0.508, 0.621, BCF$_3$ were 1.327, 1.511 and BCF$_5$ were 1.331, 1.597, respectively. BCF$_1$ values of chlorothalonil were 0.512, 0.520 and 0.619, respectively, when the concentration of carbofuran and chlorothalonil in combined treatment were 0.05+0.005, 0.05+0.010 and 0.10+0.005 ppm. BCF$_3$ values of chlorothalonil 1.341, 1.338 and 1.513, respectively, and BCF$_5$ values of chlorothalonil were 1.332, 1.327 and 1.521, respectively, under the above combined treatment. Depuration rate constants of chlorothalonil in concentration of 0.005 and 0.010 ppm under the single treatment were 0.011 and 0.012. Depuration rate constants of chlorothalonil were 0.011, 0.010 and 0.011, when the concentration of carbofuran and chlorothalonil in combined treatment were 0.05+0.005, 0.05+0.010 and 0.10+0.005 ppm. It was observed that no significant difference of carbofuran and chlorothalonil concentration in fish extracts, test water, BCFs and depuration rate constants of carbofuran and chlorothalonil between combined treatment and single treatment. It was considered that no appreciable interaction at experimental concentrations due to lower concentrations than LC$_{50}$. It is suggested that the difference of BCFs between carbofuran and chlorothalonil due to those of fat composition of fish and solubility of carbofuran and chlorothaionil.

Risk Ranger를 활용한 잠재적 위해식품과 미생물 조합에 대한 위해순위 결정

민경진,윤기선,황인균,이순호,조준일 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.2

Risk ranking must be determined for various hazards/food combinations to conduct microbial risk management effectively. Risk Ranger is a simple, easy-to-use calculation tool developed in Microsoft Excel and designed to rank the risk (low, medium, and high) for semi-quantitative microbial risk assessment. The user is required to answer 11 questions in Risk Ranger related to 1) severity of the hazard, 2) likelihood of a disease-causing dose of the hazard being present in the meal, and 3) the probability of exposure to the hazard in a defined time. This study determined the risk ranking for twenty three combinations of foodborne pathogens/potentially hazardous foods (PHFs) using a Risk Ranger. In this study, pathogenic E. coli in fresh cut produce salad was scored as 79, which was the highest rank among the 23 combinations of the foodborne pathogens and PHFs. On the other hand, zero risk was obtained with V. parahaemolyticus in sushi, Salmonella in meat products and E. coli O157:H7 in hamburger patties. Although Risk Ranger is very simple method to rate the risk of foodborne pathogens and PHFs combination, the accuracy of result was mainly affected by the availability and accuracy of data in the literature. According to the result of literature review, the data are needed for contamination rate of raw materials, consumption amount/frequency of PHFs, and the effect of processing on pathogen. Risk ranking must be continuously revalidated with new data.

Flexible PCB용 무전해 도금 Ni 박막/Polyimide 계면파괴에너지 평가

민경진,박성철,이지정,이규환,이건환,박영배,Min, Kyoung-Jin,Park, Sung-Cheol,Lee, Jee-Jeong,Lee, Kyu-Hwan,Lee, Gun-Hwan,Park, Young-Bae 한국마이크로전자및패키징학회 2007 마이크로전자 및 패키징학회지 Vol.14 No.1

폴리이미드 표면에 대한 습식 개질 전처리 조건에 따른 폴리이미드와 무전해 도금 Ni 박막사이의 계면파괴에너지를 $180^{\circ}$ 필 테스트를 통해 정량적으로 구하였다. KOH 처리시간이 1분인 경우 계면파괴에너지는 24.5 g/mm에서 5분 처리 시 33.3g/mm로 증가하였고, EDA처리 시간이 1분인 경우 31.6 g/mm에서, 5분 처리 시 22.3g/mm로 저하되었다. 이러한 습식 개질전처리 조건에 따른 폴리이미드 표면 거칠기 변화는 매우 작아서, 기계적 고착 효과는 계면파괴에너지 변화에 기여하지 못했음을 알 수 있다. KOH는 carboxyl기, EDA는 amine기를 폴리이미드 표면에 형성시켜 Ni과 강한 화학적 결합을 이루어, 폴리이미드 내부의 cohesive 박리거동을 보였다. 습식 개질전처리 조건에 따른 계면파괴에너지의 거동은 파면 부근에 형성된 O=C-O 결합과 매우 밀접한 연관성이 있는 것으로 판단된다. It is investigated how KOH and Rthylenediamine(EDA) treatment conditions on Polyimide film surface affect the interfacial fracture energy between electroless plated Ni and Polyimide film by $180^{\circ}$ peel test. Estimated values of interfacial fracture energy were 24.5 g/mm and 33.3 g/mm for the KOH treatment times under 1 and 5 minutes, respectively, while, those were 31.6 g/mm and 22.3 g/mm for EDA treatment times under 1 and 5 minutes, respectively. Interfacial bonding between electroless plated Ni and Polyimide seems to be dominated by chemical bonding effect rather than mechanical interlocking effect. It is found that chemical treatment produces carboxyl and mine functional groups which are closely related the interfacial bonding mechanism. Finally, it is speculated that interfacial fracture energy seems to be controlled by O=C-O bonding near cohesive failure region.

물산업 해외시장 진출 활성화 방안 - Water Partnership 구축을 중심으로 -

민경진,김동환,조은채,Min, Gyeong-Jin,Kim, Dong-Hwan,Jo, Eun-Chae 한국수자원학회 2011 물과 미래(한국수자원학회지) Vol.44 No.8

급성 충수염으로 진단된 예측 불가 염증성 맹장 종물에 대한 외과적 고찰

민경진,백무준,채만규,김성용,이문수,김창호,김재준,송옥평 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: An unexpected inflammatory cecal mass of uncertain etiology was encountered during surgery for presumed appendicitis. In this case, the surgeon couldn't exclude differential diagnosis which were severe appendicitis involving the cecal region, diverticular disease, other inflammatory bowel diseases, and neoplasm. Therefore, this finding leads to a therapeutic dilemma for the surgeon, when making surgical decisions during the operation. The aim of this study was to resolve this dilemma, a retrospective review was undertaken in this study. Methods: A retrospective study was performed to review the management of this problem at Soonchunhyang University Chunan Hospital for eight and half years from July 1991 to December 1999. A review of the emergency operation records identified 50 patients who had undergone ileocecal resection or right hemicolectomy for an inflammatory ileocecal mass of uncertain etiology when operated on for probable appendicitis. Results: Altogether, 39 patients underwent ileocecal resection, and 11 patients underwent right hemicolectomy. The final pathologic diagnosis was appendiceal abscess in 32 patients (63%), cecal diverticulitis in 7 patients (14%), neoplasm in 6 patients (12%), appendiceal phlegmon in 3 patients (6%), ileal tuberculosis in 1 patient (2%) and Anisakiasis in another patient (2%). The neoplasm of the 6 neoplasm patients was ascending colon cancer for 4 patients and cecal cancer for 2 patients. There was no mortality in this group. Conclusion: Although most inflammatory cecal masses are caused by benign disease, all cases in which the intraoperative diagnosis is unclear, any pathologic diagnosis including neoplasm cannot be ruled out. So this suggest ileocecal resection or right hemicolectomy to remove a possible underlying malignancy, and to decrease morbidity and mortality.

일부 농약의 생물농축계수의 측정

민경진,차춘근 한국식품위생안전성학회 1999 한국식품위생안전성학회지 Vol.14 No.2

The present study was performed to investigate the bioconcentration of BPMC, chlorothalonil, dichlorvos and methidathion. The BCFs(bioconcentration factors) and depuration rate constants for four pesticides in zebrafish(bracJxydanio rerio) were measured under semi-static conditions(OECD guideline 305-B) in a concentration of one-hundredth of the 96 hours LC_(50) of each pesticide at the equilibrium condition. The results obtained are summarized as follows : The BCFs of BPMC, chlorothalonil, dichlorvos and methidathion were 1.44±0.09, 2.223±0.063, 0.81±0.08 and 5.53±0.13, respectively. Depuration rate constants of BPMC, chlorothalonil, dichlorvos and methidathion were 0.028, 0.015, 0.220 and 0.152, respectively. The concentrations of BPMC, dichlorvos and methidathion in zebrafish reached an equilibrium in 3 days, and the equilibrium of chlorothalonil was reached after 14 days. Depuration rate of dichlorvos was the fastest followed by methidathion, BPMC and chlorothalonil. The lower BCF of BPMC was due to its relatively high K_(OW), slow K_(DEP), and low S_W and V_P, compared to chlorothalonil and methidathion. The BCF of chlorothalonil was much lower than that expected on the basis of high K_(OW) slow K_(DEP), low S_W and V_P. The reason is that the experimental concentration for chlorothalonil is 1/100 - 1/1000 lower than that of BPMC, dichlorvos and methidathion. The BCF of dichlorvos was lower than that of other pesticides due to its very rapid K_(DEP), very high V_P and S_W, and very low K_(OW). The BCF of methidathion was higher than that of other pesticides due to its very low V_P and S_W. Therefore, these data suggest that physicochemical properties of pesticides may be important in the bioconcentration.

$Pb(Zr_{0.9}Ti_{0.1})O_3$ 세라믹 Dynamic 초전특성에 관한 연구

민경진,강성준,장동훈,윤영섭,Min, Kyung-Jin,Kang, Seong-Jun,Chang, Dong-Hoon,Yoon, Yung-Sup 대한전자공학회 2000 電子工學會論文誌-SD (Semiconductor and devices) Vol.37 No.8

능면체정 구조를 갖는 $Pb(Zr_{0.9}Ti_{0.1})O_3$ 세라믹을 제작한 후, dynamic 방법으로 초전특성을 측정하여 초전형 적외선 센서의 응용 가능성에 대하여 조사하였다. $Pb(Zr_{0.9}Ti_{0.1})O_3$ 세라믹의 응답 특성이 저주파와 고주파 영역에 따른 변조 주파수의 주파수 분산 (dispersion) 으로 고려되었고, 그에 따른 초전특성의 주파수 의존성을 관찰하였다. 저주파 (2~200Hz) 영역에서, 분역의 재배향(reorientation) 으로 고려되었고 그에 다른 초전특성의 주파수의 속도보다 빠르므로 분극의 변화량이 증가하여 최대값을 나타낸다. 반면에, 고주파 (200~2000Hz) 영역에서 분역의 재배향은 주파수 증가에 따라 방해를 받아 분극의 변화량이 억제되어 초전 응답이 감소하는 것을 알 수 있다. 초전계수와 재료평가지수는 각각 $1.6{\times}10^{-8}C/cm^2{\cdot},\;1.6{\times}10^{-11}C{\cdot}cm/J$이었고, 잡음등가전력과 비검출능은 각각 $2.4{\times}10^{-7}W/Hz^{1/2},\;4.17{\times}10^6cm{\cdot}Hz^{1/2}/W$이다. Pyroelectric properties of the $Pb(Zr_{0.9}Ti_{0.1})O_3$ ceramics having the rhombohedral structure have been studied by using the dynamic measurement method. The pyroelectric responses of the $Pb(Zr_{0.9}Ti_{0.1})O_3$ ceramics are characterized in both low and high medulation frequency regions and their frequency depences are observed. In the low frequency region (2~200Hz), the change of polarization increases and shows the maximum since the reorientation rate of domains is higher than the modulation frequency. Inthe high frequency region (200~2000Hz), the pyroelectrci response decreases as the frequency increases, because the reorientation of domains is suppressed and so the change of polarization decreases. Pyroelectric coefficient, figure of merit, noise equivalent power and detectivity of the $Pb(Zr_{0.9}Ti_{0.1})O_3$ ceramics are measured as $1.6{\times}10^{-8}C/cm^2{\cdot},\;1.6{\times}10^{-11}C{\cdot}cm/J,\;2.4{\times}10^{-7}W/Hz^{1/2}\;and\;4.17{\times}10^6cm{\cdot}Hz^{1/2}/W$, respectively.