연속식 효소반응기에서 생산된 무-트랜스, 저 포화인 재구성지질의 물리화학적 특성

류현경,이기택 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2009 한국식품저장유통학회 학술대회 Vol.2009 No.-

어린이집 보육교사의 여가활동과 교사 역할수행의 관계

류현경,김정화 한국보육지원학회 2020 한국보육지원학회 학술대회지 Vol.2020 No.-

The usefulness of maternal serum C-reactive protein and lipid peroxide in the prediction of the interval between membrane rupture and delivery in preterm premature rupture of membrane

류현경,김종운,이효진,김여하,송태복,김윤하 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

The aim of this prospective study was to evaluate the usefulness of maternal serum C-reactive protein (CRP), lipid peroxide and other variables in predicting the interval between membrane rupture and delivery in patients with preterm premature rupture of membrane (PPROM). 72 patients (gestational age <34weeks) with PPROM and delivered by spontaneous vaginal delivery were analyzed. Patients were categorized into two groups; delivery within 3 days (group A) or after 3 days (group B) since PPROM. Maternal blood samples were collected at the time of admission. The levels of CRP, lipid peroxide, and protein carbonyl have been compared between group A and B. Other possible risk factors such as WBC count, amniotic fluid index (AFI), Bishop score, and cervix length on admission were also compared. CRP concentration was measured by latex agglutination method. The levels of lipid peroxide and protein carbonyl were measured by thiobarbituric acid reaction and 2, 4-dinitrophenylhydrazine method, respectively. Maternal serum CRP and lipid peroxide levels were higher in group A compared with group B (p< 0.001, and p=0.001). Bishop score was higher in group A than group B (p< 0.001). There were no statistically significant differences in WBC, neutrophil to lymphocyte ratio, protein carbonyl levels, AFI and cervix length between group A and group B. ROC curve analysis showed that CRP (cutoff value; 0.4, area under the curve=0.673, p<0.01) and lipid peroxide (cutoff value; 2.27, area under the curve=0.741, p<0.005) level on admission are predictive of delivery within 3days after PROM. CRP and lipid peroxide levels in maternal serum, and bishop score on admission may be useful to predict the interval between membrane rupture and delivery in patients with PPROM.

청보리와 청밀의 지방산 조성 및 제빵 적성 연구

류현경,이기택 동아시아식생활학회 2010 동아시아식생활학회 학술발표대회논문집 Vol.2010 No.5

양막하 혈종과 조산

최명균,김종운,류현경,김태영,박서영,방희영,조인영,조은별,김윤하 대한주산의학회 2023 Perinatology Vol.34 No.1

Most subamniotic hemorrhages occur after birth and are result from excessive traction on the umbilical cord at delivery. These lesions have been rarely reported during pregnancy. According to the previous reports, subamniotic hemorrhage has not been related to severe obstetrical complications. But in recent case reports, some authors reported fetal growth retardation and preterm birth associated with subamniotic hemorrhage. We present a case with subamniotic hemorrhage diagnosed at 25 weeks’ gestation. Her pregnancy ended in preterm birth by emergency cesarean section at 26 weeks. We report this case with a review of literature.

견과류의 지방산, 트리아실글리세롤, 토코페롤 및 파이토스테롤의 조성 연구

성민혜 ( Min Hye Sung ),류현경 ( Hyun Kyeong Lyu ),이선모 ( Sun Mo Lee ),이기택 ( Ki Teak Lee ) 한국식품저장유통학회 2010 한국식품저장유통학회지 Vol.17 No.3

효소적 에스테르 교환 반응 시 카놀라유와 대두극도경화유의 비율에 따른 저트랜스 고체지방의 특성

김영주(Young-Joo Kim),류현경(Hyun-Kyeong Lyu),이선모(Seon-Mo Lee),이기택(Ki-Teak Lee) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.9

카놀라유, 대두극도경화유를 기질로 이용하여 저트랜스 고체지방을 회분식 반응기(batch type reactor)에 의해 합성하였다. Sn-1,3 위치 특이성을 가진 TLIM을 이용한 효소적 에스테르 교환 반응을 통하여 생성된 저트랜스 고체지방의 이화학적 특성을 살펴보았다. DSC 분석을 통하여 저트랜스 고체지방의 고체지방 함량(SFC)과 융점 곡선 및 결정화 곡선을 알아보았다. 다양한 혼합비율의 저트랜스 고체지방들은 각 기질이 가진 지방산의 비율에 따라 차별적으로 넓은 범위의 온도를 나타내었다. 즉, 카놀라유의 함량이 높아질수록 각 온도에 해당하는 SFC는 감소하는 경향을 나타낸 반면, 대두극도경화유의 함량이 높아질수록 SFC가 증가하는 경향을 보였다. DSC를 이용한 흡열 및 발열 피크 분석결과, 카놀라유의 함량이 증가할수록 피크가 낮은 온도 쪽으로 이동하는 경향을 보여주었다. 저트랜스 고체지방들의 지방산조성은 C18:0와 C18:1, C18:2가 전체 지방산 조성의 80% 이상 구성되어 있으며, 총 트랜스 지방산(∑TFA)의 함량은 0.3 wt% 이하의 낮은 함량을 나타내었다. Sn-2 position의 주요 지방산 또한 C18:0와 C18:1, C18:2가 대부분을 차지하였으며 특히 불포화지방산의 함량이 높게 나타났다. 역상 HPLC를 이용하여 TAG 조성을 분석한 결과, 주요 TAG 조성은 LOO, OOO, POO/SOL, SOO, SOS로 구성되어 있었다. 그중 카놀라유의 함량이 증가할수록 저트랜스 고체지방의 TAG 조성은 LOO, OOO, POO/SOL, SOO의 수치가 증가하였고, 대두극도경화유의 함량이 감소할수록 POS/PSP, SOS, SSS의 수치는 줄어드는 것을 확인할 수 있었다. Lipase-catalyzed interesterification of canola (CO) and fully hydrogenated soybean oil (FHSBO) at different weight ratios (70:30, 75:25, and 80:20) was performed in a batch type reactor to produce low-trans solid fats. Each reaction was conducted in the shaking water bath for various reaction times (1, 3, 6, 18 and 24 hr) at 70oC and 220 rpm using Lipozyme TLIM (20 wt% of total substrate) from Thermomyces lanuginosus. After 24 hr reaction, solid fat content (SFC) by differential scanning calorimetry (DSC), fatty acid and triacylglycerol (TAG) composition of low-trans solid fats were determined. SFC of the products was reduced when the content of canola oil in the reaction mixture was increased. Major fatty acids were stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2). Trans fatty acid content in the low-trans solid fats showed less than 0.3 wt%. In the HPLC analysis, major TAG species showed LOO (linoleyl-oleoyl-oleoyl), OOO, POO/SOL, SOO, and SOS.

카놀라유와 대두극도경화유로부터 효소적으로 합성된 저트랜스 스프레드 고체지의 특성

김영주(Young-Joo Kim),류현경(Hyun-Kyeong Lyu),신정아(Jung-Ah Shin),이기택(Ki-Teak Lee) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.9

카놀라유와 대두극도경화유를 기질로 이용하여 저트랜스 스프레드 제조 시에 사용할 수 있는 LTSF을 효소적 inter-esterification반응을 이용하여 합성하였고, 제조된 LTSF의 온도별 SFC 함량, 반응 후 TAG 조성의 변화 및 결정형을 살펴보았다. DSC를 이용한 SFC 분석결과, LTSF와 시중에서 유통되는 스프레드로부터 획득한 고체지간에 약 20~35℃의 범위에서 유사한 고체지 함량을 보임에 따라 합성된 LTSF는 적합한 물성을 가지는 스프레드 제조 시에 사용할 수 있을 것으로 사료된다. LTSF의 지방산 조성은 C18:0(29.2 wt%), C18:1(41.8 wt%), C18:2(13.3 wt%)가 전체 지방산 조성의 80% 이상 구성되어 있으며, 총 트랜스 지방산(∑TFA)의 함량은 시중 유통 스프레드 고체지보다 훨씬 적은 0.2 wt%로 미량 검출되었다. Sn-2 position에 분포된 지방산 조성은 C18:0(31.5 wt%), C18:1(41.4 wt%), C18:2(12.1 wt%)로 구성되었고, sn-1,3 position에 위치한 지방산 조성도 C18:0(28.1 wt%), C18:1(42.1 wt%), C18:2(13.9 wt%)로 구성되었다. Reversed-phase HPLC 분석결과, 카놀라유는 LOO와 OOO로 구성되어 있었으며, 대두극도경화유는 PSS와 SSS의 주된 TAG 조성을 나타내었다. 두 기질을 사용하여 대량 합성된 LTSF의 반응 전후 TAG 조성을 비교해 보면, LOO와 OOO, SSS는 반응 후 그 수치가 줄어든 반면, SOL과 SOO는 각각 2.3에서 19.4 area%로, 0.5에서 26.4 area%로 증가하였으며, 새로운 TAG로서 POS/PSP와 SOS가 각각 12.6 area%와 16.5 area% 정도 생성되었다. LTSF의 결정형태를 알아보기 위하여 polarized light microscopy와 x-ray diffraction 분석을 수행한 결과, 결정크기는 작고 조밀하여 부드러운 조직감을 나타낼 것으로 생각되며 일반적으로 마가린이 β'형일 때 바람직하다고 보고된 바와 같이, 합성한 LTSF은 β형도 공존하지만 β'형이 우세하게 나타내어 스프레드의 물성에 적합할 것으로 사료된다. Low-trans spread fat (LTSF) was produced by lipase-catalyzed synthesis of canola (CO) and fully hydrogenated soybean oil (FHSBO) at 65:35 (w/w). Blend of CO and FHSBO with 65:35 ratio was interesterified using Lipozyme TLIM (immobilized Thermomyces lanuginosus, 20% of total substrate) in a 1 L-batch type reactor at 70℃ with 500 rpm for 24 hr. Then, physicochemical melting properties of LTSF were compared with commercial spread fat. At 20℃, solid fat contents (SFC) of commercial spread fat as a control and LTSF were similar, showing 19.1 and 18.1%, respectively. Major compositional fatty acids of LTSF were C18:0, C18:1 and C18:2 (29.2, 41.8 and 13.3 wt%, respectively). Trans fatty acid content of the LTSF (0.2 wt%) was lower than that of commercial spread fat (5.5 wt%). In the RP-HPLC analysis from LTSF, major triacylglycerol (TAG) molecules were SOL (stearoyl-oleoyl-linoleyl), SOO, POS/PSP, and SOS. Also, polymorphic form and x-ray diffraction of LTSF showed coexistence of β' and β form crystals.

Regulation of Hepatic Gluconeogenesis by Nuclear Receptor Coactivator 6

오경식,김시령,이은숙,윤진,류현경,김동섭,Seung-Whan Kim,Min-Kyung Shin 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.4

Nuclear receptor coactivator 6 (NCOA6) is a transcriptional coactivator of nuclear receptors and other transcription factors. A general Ncoa6 knockout mouse was previously shown to be embryonic lethal, but we here generated liver-specific Ncoa6 knockout (Ncoa6 LKO) mice to investigate the metabolic function of NCOA6 in the liver. These Ncoa6 LKO mice exhibited similar blood glucose and insulin levels to wild type but showed improvements in glucose tolerance, insulin sensitivity, and pyruvate tolerance. The decrease in glucose production from pyruvate in these LKO mice was consistent with the abrogation of the fasting-stimulated induction of gluconeogenic genes, phosphoenolpyruvate carboxykinase 1 (Pck1) and glucose-6-phosphatase (G6pc). The forskolin-stimulated inductions of Pck1 and G6pc were also dramatically reduced in primary hepatocytes isolated from Ncoa6 LKO mice, whereas the expression levels of other gluconeogenic gene regulators, including cAMP response element binding protein (Creb), forkhead box protein O1 and peroxisome proliferator-activated receptor γ coactivator 1α, were unaltered in the LKO mouse livers. CREB phosphorylation via fasting or forskolin stimulation was normal in the livers and primary hepatocytes of the LKO mice. Notably, it was observed that CREB interacts with NCOA6. The transcriptional activity of CREB was found to be enhanced by NCOA6 in the context of Pck1 and G6pc promoters. NCOA6-dependent augmentation was abolished in cAMP response element (CRE) mutant promoters of the Pck1 and G6pc genes. Our present results suggest that NCOA6 regulates hepatic gluconeogenesis by modulating glucagon/cAMP-dependent gluconeogenic gene transcription through an interaction with CREB.

투광점 안정화를 적용한 보행동결 대응용 두 줄 레이저 지팡이 개발

홍석민(S. M. Hong),서동권(D. K. Seo),류현경(H. K. Ryu),이진복(J. B. Yi) 한국재활복지공학회 2023 재활복지공학회논문지 Vol.17 No.2