Modulation of Electroosmotic Flow through Skin: Effect of Poly(Amidoamine) Dendrimers

김혜지,오승열 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.2

The objective of this work is to evaluate the effect of polyamidoamine (PAMAM) dendrimers on electroosmotic flow (EOF) through skin. The effect of size and concentration of dendrimer was studied, using generation 1, 4 and 7 dendrimer (G1, G4 and G7, respectively). As a marker molecule for the direction and magnitude of EOF, a neutral molecule, acetoaminophen (AAP) was used. The visualization of dendrimer permeation into the current conducting pore (CCP) of skin was made using G4–fluorescein isothiocyanate (FITC) conjugate and confocal microscopy. Without dendrimer, anodal flux of AAP was much higher than cathodal or passive flux. When G1 dendrimer was added, anodal flux decreased, presumably due to the decrease in EOF by the association of G1 dendrimer with net negative charge in CCP. As the generation increased, larger decrease in anodal flux was observed, and the direction of EOF was reversed. Small amount of methanol used for the preparation of dendrimer solution also contributed to the decrease in anodal flux of AAP. Cross-sectional view perpendicular to the skin surface by confocal laser scanning microscope (CLSM) study showed that G4 dendrimer-FITC conjugate (G4-FITC) can penetrate into the viable epidermis and dermis under anodal current. The permeation route seemed to be localized on hair follicle region. These results suggest that PAMAM dendrimers can permeate into CCP and change the magnitude and direction of EOF. Overall, we obtained a better understanding on the mechanistic insights into the electroosmosis phenomena and its role on flux during iontophoresis.

대학 합성생물학 실습수업용 프로그램 개발 - 유전자 회로 조립 실험과 빛 테두리 그림 그리기 실험을 바탕으로 -

김혜지,송준현,김아람 한국생물교육학회 2019 생물교육 Vol.47 No.4

The purpose of this study is to develop a practical training program for synthetic biology that introduces biology major students to the field of synthetic biology and enables students to experience an exciting part of the synthetic biology research. This program consists of two hands-on modules. The first module constructs a gene circuit that produces red fluorescent proteins, allowing students to observe the result directly through their eyes. In the second module, students learn common molecular logic of gene circuits by practicing bacterial programming that enables bacteria to detect the light border. This practice program was applied to the biology major students, and their perception of the contents of the program was measured. Learning assessment quizzes revealed that ~87%(7/8) of the participants well understood the key concepts in this practice program. In addition, all participants, except one, responded that the experimental design to visually check whether a genetic circuit works increased their interest in the field of synthetic biology. The results show the possibility of this program as an introductory practice course for undergraduate synthetic biology education.

LabView와 X-Plane Simulator를 활용한 무인항공기 비행알고리듬 검증용 PILS 구현

김혜지,오승조,도지호,장현천,이동진 한국항공우주학회 2015 한국항공우주학회 학술발표회 논문집 Vol.2015 No.11

본 논문에서는 무인항공기 비행제어 알고리듬 검증을 위한 PILS를 개발하였다. 본 논문에서의 PILS는 무인기 검증에 적합하도록 개발하기 위해 시험환경의 범용성 및 시험 데이터의 정확성을 높이는데 중점을 두었다. 비행시뮬레이션 프로그램으로 실제 환경과 유사한 환경을 제공하는 X-Plane Simulator를 사용하여 현실성 높은 시험환경을 구축하였다. X-Plane Simulator와 Flight Control Computer의 연동은 Simulator내의 UDP통신과 Serial통신을 이용하여 LabView를 통해 연동하였다. GUI기반의 LabView를 이용하여 개발환경을 구축함으로써 개발시간 단축 및 단기간 수정이 가능하여 시스템의 효율성을 높였고, 실시간으로 X-Plane으로의 사용자 명령 입력, 비행제어컴퓨터로 파라미터 및 모드 등을 입력할 수 있는 기능을 추가하여 시험환경의 효율을 증가시켰다. PILS in this paper is developed focusing to enhance the generality of the test environment and accuracy of the test data for verification of UAVs. By using the X-Plane Simulator to provide an environment similar to the actual environment, it enhanced reality of the test environment. Interlocking X-Plane Simulator and Flight Control Computer is linked via LabView using the UDP communication and Serial communication. By building up a development environment using Labview, it increased the efficiency of the system to facilitate to shorten the development time and to modify data. And adding the function to input a user command to X-Plane, parameters and modes to flight control computer in real time increased the efficiency.

Effect of Veterinary Antibiotics on the Growth of Lettuce

김혜지,이승현,홍영규,김성철 한국토양비료학회 2018 한국토양비료학회지 Vol.51 No.2

Veterinary antibiotics (VAs) has been used to treat animal disease and to increase body weight. However,released VAs in the soil via spreading of compost can transport to plant and affect its growth. Main purpose ofthis research was i) to monitor VAs concentration in plant and ii) to evaluate inhibition effect of VAs residualson the plant growth. Red lettuce (Lactuca sativa) was cultivated for 35 days in the pot soil spiked with 3different concertation (0.05, 0.5, 5.0 mg kg-1) of chlortetracycline (CTC) and sulfamethazine (SMZ). After 35days of cultivation, concentration of CTC and SMZ in the plant was measured. Residual of CTC and SMZ wasonly quantified at the range of 0.007-0.008 mg kg-1 and 0.006-0.017 mg kg-1 in the leaf and root respectivelywhen high concentration (5.0 mg kg-1) of antibiotic was spiked in the soil. Leaf length and root mass wasstatistically reduced when 0.05 mg kg-1 of CTC was spiked in the soil while no statistical difference wasobserved for SMZ treatment. This result might indicated that high Kow and Kd value are the main parametersfor inhibiting plant growth. Antibiotics that has a high Kow causing hydrophobicity and easy to bioaccumulatein the lipid cell membrane. Also, antibiotics that has a high Kd properties can be sorbed in the root causinggrowth inhibition of the plant. Overall, management of VAs should be conducted to minimize adverse effectof VAs in the ecosystem.

Comparison of Tropical Cyclone Wind Radius Estimates between the KMA, RSMC Tokyo, and JTWC

김혜지,문일주,오임영 한국기상학회 2022 Asia-Pacific Journal of Atmospheric Sciences Vol.58 No.4

This study compared estimates of gale-force wind radii (R30 or R34) and storm-force wind radii (R50) of tropical cyclones(TC) by three agencies—the Korea Meteorological Administration (KMA), the Regional Specialized Meteorological Center(RSMC) Tokyo, and the Joint Typhoon Warning Center (JTWC)—in the western North Pacific during 2015–2018 and investigatedthe characteristics of these estimates. The results showed that the KMA’s R30 and R50 estimates were smaller (38%and 29%, respectively) than those of the RSMC Tokyo, and larger (11%) for R30 and smaller (12%) for R50 than those of theJTWC. The differences between these agencies seem to be largely determined by whether the agency estimates wind radiibased only on a TC’s own winds or on TC winds combined with other mid-latitude synoptic systems to make TC warningsmore comprehensive. The former is mainly the practice of the KMA and JTWC, whereas the latter is mainly the practice ofthe RSMC Tokyo. The factors considered for estimating wind radii also differ between the agencies: the KMA heavily relieson TC intensity—the higher the intensity, the larger the radius—while the RSMC Tokyo and JTWC rely less on TC intensitythan the KMA but additionally consider the latitude and storm translation speed in their estimations. In particular, the TCtranslation speed considered by the RSMC Tokyo and JTWC explains why their estimated wind radii exhibit, on average,greater asymmetries (i.e., greater differences between the longest and shortest radii) than those estimated by the KMA. Thefindings of agency-dependent characteristics of TC wind radius data help to better determining and understanding the TCimpact areas for TC risk reduction and management.

NPU 반도체를 위한 저정밀도 데이터 타입 개발 동향

김혜지,한진호,권영수,Kim, H.J.,Han, J.H.,Kwon, Y.S. 한국전자통신연구원 2022 전자통신동향분석 Vol.37 No.1

With increasing size of transformer-based neural networks, a light-weight algorithm and efficient AI accelerator has been developed to train these huge networks in practical design time. In this article, we present a survey of state-of-the-art research on the low-precision computational algorithms especially for floating-point formats and their hardware accelerator. We describe the trends by focusing on the work of two leading research groups-IBM and Seoul National University-which have deep knowledge in both AI algorithm and hardware architecture. For the low-precision algorithm, we summarize two efficient floating-point formats (hybrid FP8 and radix-4 FP4) with accuracy-preserving algorithms for training on the main research stream. Moreover, we describe the AI processor architecture supporting the low-bit mixed precision computing unit including the integer engine.

ICD-11의 국내외 주요 이슈 및 향후 과제

김혜지,안미선,이여진,우혜경 한국보건정보통계학회 2022 보건정보통계학회지 Vol.47 No.4

The 11th revision the International Statistical Classification of Diseases and Related Health Problems (ICD) is new classification system suitable for the pace of development of modern technology. Computerization and ontology-based design are expected to be highly scalable and interoperable in inte- gration and connection with other classification systems. However, when introduced in Korea, structural changes are feared to interfere with the linkage between disease codes. This study briefly introduced ICD-11 and examined related literature and mapping case trends. The interest in ICD-11 in Korea was lower than in other countries, and tend to pay attention to socio-economic repercussions. Research was mainly conducted focusing on ‘Chapter 2 Neoplasms’ and ‘Chapter 6 Mental, behavioural or neurodevelopmental disorders’. Through a mapping case study, it was confirmed that ICD-11 is a highly useful classification system, and guidance on post-coordination, such as the sequence and criteria of the expansion code, is needed. Proper preparation is required until ICD-11 is officially implemented in Korea. The role of the government is important at all stages from the introduction to maintenance of ICD-11, and efforts and attention to adaptation of all classified users in the health care industry are needed. .

내현적 자기애, 수치심, 우울이 대학생의 폭식에 미치는 영향

김혜지,이민규 한국청소년학회 2020 청소년학연구 Vol.27 No.12

The purpose of this study was to identify the mediating effect of depression, shame in the relationships between covert narcissism and binge-eating. The subjects of this study were 419 university students(male 189, female 230) in Gyeongsangnam-do. They were assessed by Covert narcissism scale, Experience of shame scale, Korea depression scale, The korean version of eating attitudes test-26, which were analyzed through Structural Equation Modeling(SEM). In addition, SEM multi-group analyses were used to verify differences among male and female groups to identify gender-specific moderating effects. The result of this study were as follows: first, binge-eating was a significant static positive correlation between covert narcissism, depression, shame. Second, the path of cover narcissism and binge-eating was verified by the SEM, and it was found that intrinsic covert narcissism completely mediated depression or shame, thus affecting binge-eating. In other words, a high level of inner covert narcissism leads to more negative emotions such as depression and shame, which increases binge-eating behavior. Third, SEM multi-group(male/female) analyses revealed significant difference in two group was found for one of the three paths(shame→binge-eating). Finally, implications and limitations of this study were discussed. In addition, suggestions for future study were presented. 본 연구는 내현적 자기애, 우울, 수치심과 폭식 간의 관계를 알아보고, 내현적 자기애가 우울과 수치심을 매개하여 폭식에 미치는 영향을 검증하였다. 경남지역 대학생 419명이 연구에 참여하였다(남성 189, 여성 230). 측정도구로는 내현적 자기애 척도, 수치심 경험 척도, 한국 우울증 척도, 한국판 식사태도검사가 사용되었으며, 구조방정식 모형을 통해 분석하였다. 또한 성별에 따른 조절효과를 알아보기 위해 3단계 다집단 분석을 사용하여 남녀 집단에서 차이를 검증하였다. 연구 결과는 다음과 같다. 첫째, 폭식행동은 내현적 자기애, 우울, 수치심과 모두 유의한 정적 상관을 나타내었다. 이는 내현적 자기애가 높고 우울과 수치심을 많이 느낄수록 폭식행동이 많이 나타난다는 것을 의미한다. 둘째, 구조방정식 모형을 통해 내현적 자기애와 폭식 간의 경로를 검증해 본 결과, 내현적 자기애는 우울 또는 수치심을 완전 매개하여 폭식에 영향을 미치는 것으로 나타났다. 즉, 내현적 자기애가 높은 사람들은 우울 및 수치심과 같은 부정정서를 더 많이 경험하며 이는 폭식행동을 증가시킨다. 셋째, 연구모형에 대한 남녀 집단 간 차이 검증은 수치심→폭식 경로에서만 유의한 차이가 났다. 마지막으로 본 연구의 의의와 제한점, 후속 연구에 대해 논의하였다.

YEST 4기 활동을 마무리하며···

김혜지 전략물자관리원 2015 전략물자 focus Vol.- No.12

노인장기요양 현금급여 통제정책에 대한 국가 간 비교연구- 독일, 스웨덴, 영국을 중심으로 -

김혜지,최영,김남연 한국지역사회복지학회 2022 한국지역사회복지학 Vol.- No.81

The purpose of this study is to compare and analyze cash-for-care regulatory policies for the elderly long-term care in three countries, Germany, Sweden, and the UK, and to seek policy implications in preparation for regulatory policies that are suitable for the direction of South Korea’s welfare state and the socio-cultural environment. For this purpose, each country’s cash-for-care regulatory policy was analyzed in four different aspects: supplier designation, service provider qualification, use of cash benefit, and professional support. As a result, it was confirmed that cash-for-care regulatory policies of the three countries are being operated differently depending on the institutional and socio-cultural characteristics of each country. Through this analysis, it is suggested to primarily apply the regulatory policy of the Swedish model, which provides support by individual cases, and then to apply the German model, which offers active professional support, in the future when the group of the cash benefit recipient expands. To this end, it is suggested that there should be active discussions for the cash-for-care regulatory policy of South Korea to be prepared in a direction suitable for the policy context and intention of the institution, and ultimately, an official service system through case management should be established. 본 연구의 목적은 독일, 스웨덴, 영국 3개국에서 실시하고 있는 노인장기요양 현금급여에 대한 통제정책을 비교 분석하고, 한국의 복지국가 방향에 적합한 통제정책 마련을위한 정책적 시사점을 모색하는 것이다. 이를 위하여 각 국가의 현금급여 통제정책을 공급자 지정, 서비스 제공자격, 현금급여 이용, 전문적 지원의 4가지 측면에서 분석하였다. 그 결과, 3개국의 현금급여 통제정책은 각국의 제도적 및 사회문화적 특성에 따라 상이하게 나타나고 있음을 확인하였다. 이러한 분석결과를 바탕으로 일차적으로는 개별 케이스를 중심으로 지원하는 스웨덴 모델의 통제정책을 적용하고, 향후 급여대상의 규모가확대될 경우 전문적 지원이 활성화되어 있는 독일 모델의 적용을 제안하였다. 이를 위해우리나라의 현금급여 통제 방안이 제도의 정책적 맥락 및 의도에 적합한 방향으로 마련되기 위한 논의가 활발하게 이루어져야 하며, 궁극적으로 사례관리 중심의 공적 지원 체계가 구축되어야 함을 제시하였다.