Saccharomyces cerevisiae에서 Cycloinulooligosaccharide Fructanotransferase 유전자의 표층 발현

김현진,이재형,김현철,김연희,권현주,남수완,Kim, Hyun-Jin,Lee, Jae-Hyung,Kim, Hyun-Chul,Kim, Yeon-Hee,Kwon, Hyun-Ju,Nam, Soo-Wan 한국생명과학회 2007 생명과학회지 Vol.17 No.2

Paenibacillus macerans 유래의 cycloinulooligosaccharide fructanotransferase (CFTase) 유전자(cft, 2832 bp, 103.8 kDa)를 효모 표층발현 vector인 pCTcon (GAL1 promoter)에 subcloning 하였다. 구축된 pCTECFTN (9.0 kb)를 숙주세포인 s. cerevisiae EBY100에 형질전환한 후, uracil이 결핍된 배지와 inulin 함유배지에서 선별하였다. cft 유전자는 GAL1 promoter에 의해 효모 형질전환체에서 성공적으로 발현되었다. inulin으로부터 cyclofructans (CFs)로 생산하는 효소적 능력으로부터 표층발현 유무를 확인하였다. YPDG배지에서 48시간 배양 후 분획한 균체는 5.52 unit/ l 의 활성을 보였다. CF 생산을 위한 효소의 최적 반응 조건으로 pH 8.0, 반응온도 $50^{\circ}C$, 기질농도 5%, 기질은 Jerusalem artichoke 등의 inulin과 표층 발현 CFTase 효소반응 결과, cycloinulohexaose (CF6), cycloinuloheptaose (CF7), 그리고 cycloinulooctaose (CF8)이 생성되었고, 이 중에서 CF6가 주 생성물이었다. The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTECFTN (9.0 kb) was introduced to S. cerevisiae EBY100 cell and then east transformants were selected on the synthetic defined medium lacking uracil and on the inulin containing medium. The surface display of CFTase was confirmed by immunofluorescence microscopy and its enzymatic ability to form cycloinulooligosaccharides(cyclofructans, CFs) from inulin. The total activity of the CFTase was reached about 5.52 unit/1 by cultivation of yeast transformant on YPDG medium. The optimized conditions determined were as follows; pH, 8.0; temperature, $50^{\circ}C$ ; substrate concentration, 5%; inulin source, Jerusalem artichoke. By the reaction with inulin, CFs consisting of cycloinulohexaose (CF6), cycloinuloheptaose (CF7), and cycloinulooctaose (CF8) were produced and CF6 was the major product.

감모후유증(感冒後遺症)으로 기허형(氣虛型)호흡기 질환을 호소하는 환자의 한약치료에 관한 임상연구

김현진,한효정,장정아,박은영,안태한,서호석,김진원,Kim, Hyun-Jin,Han, Hyo-Jung,Jang, Jeong-A,Park, Eun-Young,An, Tae-Han,Seo, Ho-Seok,Kim, Jin-Won 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

Objectives : In order to confirm a remedial effect and related influence of the clinic treatment by prescribing Samchuljojung-tang(蔘朮調中湯), Bojungikqi-tang(補中益氣湯) and Samso-um(蔘蘇飮) to patients who suffering from a chronic cough and rhinorrhea. Methods : 1. In a clinical observation and analysis method, we have selected the 23 patients who have visited National Oriental Medical Center, date from 2007.8.21 to 2008.7.31 and suffering from a chronic cough and rhinorrhea caused by the common cold sequelae. 2. Separated them into three types(I: cough type, II: rhinorrhea type, III: cough & rhinorrhea type). 3. Observed thoroughly the level of symptom and recorded it for each type with questionnaire survey at the beginning set 4. Prescribed Samchuljojung-tang, Bojungikqi-tang and Samso-um for type I, II, III respectively. 5. Observed the level of symptom and treatment afterwards affect, and recorded movements of intensity level for 3 types with same questionnaires. Results : 1. Type I : The survey turned out to be from 9 patients, initial level recorded 31.89 at the entry diagnosis, and next level that means changing of symptoms, recorded to 12.67 after prescribing herb medication. 2. Type II : From 11 patients, initial level was 32.82 and next level was 13.45. 3. Type III : From 3 patients, initial level was 37.67 and next one was 16.00. Conclusions : It is concluded that there is a significant remedial effect and related influence of the clinic treatment for each type of patients who suffering from a chronic cough and rhinorrhea caused by the common cold sequelae.

Burden of Disease Due to Outdoor Air Pollution in Korea: Based on PM₁₀

김현진,윤석준,김형수,이건세,김은정,조민우,오인환,Kim, Hyun-Jin,Yoon, Seok-Jun,Kim, Hyeong-Su,Lee, Kun-Sei,Kim, Eun-Jung,Jo, Min-Woo,Oh, In-Hwan Korean Society of Environmental Health 2011 한국환경보건학회지 Vol.37 No.5

Purpose: This study measured the burden of disease in Korea related to outdoor air pollution using disability-adjusted life year (DALY). Materials and Methods: As a risk factor of outdoor air pollution, particulate matter with a diameter less than 10 ${\mu}m$($PM_{10}$) was used. First, $PM_{10}$-related diseases and their relative risk (RR) were selected by means of a literature review. Second, population attributable fractions were computed by using formulae including RR and population exposure to $PM_{10}$. Third, DALYs of $PM_{10}$-related diseases in Korea were estimated. Finally, the attributable burden of disease due to $PM_{10}$ was measured as the sum of the products that multiplied the DALYs of $PM_{10}$-related diseases by their population attributable fractions. Results: The disease burden of PM10 was 6.9 DALY per 1,000 persons in 2007. The attributable burden of $PM_{10}$ was 2.68 for lung cancer, 2.41 for COPD, 0.62 for ischemic heart disease, 0.61 for pneumonia, 0.55 for asthma, and 0.03 for preterm. Conclusions: This study showed the environmental burden of disease of $PM_{10}$ and burden of $PM_{10}$-related disease through objective data. It also suggested that active efforts are needed to continuously measure and reduce the burden of environmental diseases in Korea.

Dieulafoy 병변 소아의 내시경적 치료 1례

김현진,신지선,서정완,Kim, Hyun-Jin,Shin, Jee-Seon,Seo, Jeong-Wan 대한소아소화기영양학회 2003 Pediatric gastroenterology, hepatology & nutrition Vol.6 No.2

Dieulafoy 병변은 비정상적으로 확장된 점막하 동맥이 소화관 내로 노출되어 발생하는 병변으로 위장관 출혈의 드문 원인이다. 저자들은 폐렴으로 입원 중 다량의 토혈과 혈변을 보인 9세 남아에서 상부 소화관 내시경으로 Dieulafoy 병변을 진단하고 순수 에탄올 국소 주사법으로 성공적으로 치료하였기에 문헌 고찰과 함께 보고하는 바이다. The Dieulafoy's lesion is a rare cause of recurrent massive gastrointestinal bleeding in children. The bleeding results from an abnormally large submucosal artery that protrudes through a small mucosal defect. The lesion is commonly found on proximal stomach. Surgical intervention was believed to be the best treatment in the past, but recent advancement in endoscopy has made effective hemostasis possible. We report a case of a 9-year-old boy with underlying mycoplasma pneumonia with effusion who presented with massive upper gastrointestinal bleeding. Bleeding was controlled with endoscopic treatment by epinephrine and ethanol injection and the patient was successfully treated.

반성적 마이크로티칭과 비원어민 예비 영어 교사의 외국어 교수 불안감

김현진,Kim, Hyun-Jin 한국영어어문교육학회 2009 영어어문교육 Vol.15 No.4

The present data-driven study attempted to explicate nonnative English teacher trainees' foreign language teaching anxiety in microteaching settings from their perspectives. It is assumed that nonnative English teachers or teacher trainees may experience anxiety not only as foreign language learners but also as foreign language teachers. In order to inquire into their anxiety, the researcher had 172 teacher trainees perform extended microteaching tasks and reflect on their teaching and anxiety through group discussion. Based on the analysis of their discussion, three aspects related to nonnative English teacher trainees' anxiety were identified. First, teacher trainees identified three main types of anxiety-provoking situations: communicative-competence-threatening situations, unexpected situations, and instruction-hindering situations. Second, they identified three sources of anxiety: limited ability to use English, lack of English teaching skills, and fear of criticism. Third, they were aware that they used diverse strategies to lower anxiety before and while teaching for different purposes. From their identification and awareness of anxiety-provoking situations, sources of anxiety, and anxiety-lowering strategies, they could reflect on professional qualifications as a foreign language teacher.

농촌노인 여가복지시설 이용 실태 및 만족도에 관한 연구 - 의성군 지역을 중심으로 -

김현진,김용범,Kim, Hyun-Jin,Kim, Yong-Bum 한국주거학회 2010 한국주거학회 논문집 Vol.21 No.5

Although the government financial aid is insufficient and the lack of programs and facilities prevent it from developing and activation in the rural local society, welfare centers for the aged are formed an ever-greater part of the leisure and welfare facility for the aged in rural areas. As a result, the objectives of this study are to investigate and identify the actual conditions of use by the aged and the degree of satisfaction for welfare centers for the aged in Uiseong district. The results of this study are as follows. In the survey sample characteristics based on a total of 303 survey, the percentage of women (55.2%) was much higher than men (44.8%), average age is 73.3 years old. According to the results of the analysis, most important motivation is for health and making friends, and major useful facilities and programs are related to physical activities, sing a song and rehabilitation. From the results of the overall satisfaction, positive promotions and sufficient aid are required for the aged in the rural local society. Further any programs developed internally are required to promote and activate the leisure activity programs for the aged.

외과적 복부질환으로 오인된 결핵성 복막염 1례

김현진,김혜순,서정완,최금자,이선화,성순희,Kim, Hyun-Jin,Kim, Hae-Soon,Seo, Jeong-Wan,Choi, Kum-Ja,Lee, Sun-Wha,Sung, Sun-Hee 대한소아소화기영양학회 2002 Pediatric gastroenterology, hepatology & nutrition Vol.5 No.2

저자들은 지속되는 고열과 복통을 주소로 입원한 10세 남아에서 외과적인 복부 질환이 의심되어 시험적 개복술을 시행하여 복막 조직 검사 소견과 복수에서 결핵균의 배양으로 확진하고 항결핵제 투여로 치료한 결핵성 복막염 1례를 문헌고찰과 함께 보고하는 바이다. Childhood tuberculous peritonitis is difficult to diagnose especially in cases without pulmonary involvement. It may present as mechanical ileus, perforation, simulating acute appendicitis, enterocolitis or intusussception. Early diagnosis in children may be difficult, largely because of variable vague symptoms and nonspecific signs. Surgery has often been required for pathologic confirmation. We have experienced a case of tuberculous peritonitis presenting with abdominal pain, abdominal distension and persistent high fever in a 10-year-old boy who was diagnosed by explo-laparotomy and pathologic confirmation from biopsy specimen from omentum. The patient was treated with antituberculous drugs and recovered uneventfully.

다중 주사 경로 회로 기판을 위한 내장된 자체 테스트 기법의 연구

김현진,신종철,임용태,강성호,Kim, Hyun-Jin,Shin, Jong-Chul,Yim, Yong-Tae,Kang, Sung-Ho 대한전자공학회 1999 電子工學會論文誌, C Vol.c36 No.2

The IEEE standard 1149.1, which was proposed to increase the observability and the controllability in I/O pins, makes it possible the board level testing. In the boundary-scan environments, many shift operations are required due to their serial nature. This increases the test application time and the test application costs. To reduce the test application time, the method based on the parallel opereational multiple scan paths was proposed, but this requires the additional I/O pins and the internal wires. Moreover, it is difficult to make the designs in conformity to the IEEE standard 1149.1 since the standard does not support the parallel operation of data shifts on the scan paths. In this paper, the multiple scan path access algorithm which controls two scan paths simultaneously with one test bus is proposed. Based on the new algorithm, the new algorithm, the new board level BIST architecture which has a relatively small area overhead is developed. The new BIST architecture can reduce the test application time since it can shift the test patterns and the test responses of two scan paths at a time. In addition, it can reduce the costs for the test pattern generation and the test response analysis. 인쇄 회로 보드 수준의 테스팅을 위해 제안된 IEEE 표준 1149.1은 보드상의 테스트 지점에 대한 제어용이도와 관측용이도를 향상시켜 보드의 테스트를 용이하게 해준다. 그러나, 경계 주사 환경에서는 테스트 입력과 테스트 결과에 따른 데이터가 하나의 주사 연결에 의해서 직렬로 이동된다. 이는 테스트 적용시간을 증가시키고 따라서 테스트에 드는 비용을 증가시킨다. 테스트에 소모되는 시간을 줄이기 위해 병렬로 다중주사 경로를 구성하는 방법이 제안되었다. 하지만 이는 여분의 입출력 핀과 내선을 필요로 한다. 더구나 IEEE 표준 1149.1은 주사 경로 상에 있는 IC들의 병렬 동작을 지원하지 않기 때문에 표준에 맞게 설계하기가 어렵다. 본 논문에서는 하나의 테스트 버스로 두 개의 주사 경로를 동시에 제안하는 다중 주사 경로 접근 알고리즘에 기초하여 적은 면적 오버헤드를 가지고 빠른 시간 내에 보드를 테스트할 수 있는 새로운 보드수준의 내장된 자체 테스트 구조를 구현하였다. 제안된 내장된 자체 테스트 구조는 두 개의 주사 경로에 대한 테스트 입력과 테스트 결과를 이동시킬 수 있으므로 테스트에 소모되는 시간을 줄일 수 있고 또한 테스트 입력의 생성과 테스트 결과의 분석에 소모되는 비용을 줄일 수 있다.

케틀벨 운동과 초음파 치료가 20대 성인의 체질량지수, 체지방률에 미치는 영향

김현진,김명훈,Kim, Hyun-Jin,Kim, Myung-Hoon 대한물리치료과학회 2018 대한물리치료과학회지 Vol.25 No.2

Background: This study was to examine the influence of BMI and PBF of adults in 20's through the Kettlebell exercise and ultrasound for 4 weeks. Methods: 24 adults men and women, whose BMI is over 23, was divided into 3 groups, group I (control group), group II (Kettlebell exercise group), group III (ultrasound treatment and Kettlebell exercise group). The ultrasound treatment was done before exercise for 15 minutes 3 times a week for 4 weeks, and exercise worked out with Kettlebell exercise during 15 minutes and were stretched for 10 minutes before and after exercise. Results: For 4 weeks of Kettlebell exercise and ultrasound, there was a statistically meaningful difference between group I and group III (p<.05). For 4 weeks, there was no statistically meaningful difference of BMI and PBF between group II and group. I Conclusion: A complex application of Kettlebell exercise and ultrasound treatment was thought to effective decreasing for BMI and PBF.

가와사끼병에서 면역글로불린 조기치료의 안전성과 효과

김현진,염혜원,김혜순,손세정,Kim, Hyun Jin,Yom, Hae Won,Kim, Hae Soon,Sohn, Sejung 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.10

Purpose : To determine the differences in clinical characteristics, blood chemistry and coronary artery complications between patients with Kawasaki disease who received intravenous immunoglobulin (IVIG) within the fourth day of illness and after the fifth day of illness. Methods : A retrospective chart review was conducted of all children with Kawasaki disease who were admitted to Ewha Mokdong Hospital between January 2001 and June 2002. The early treatment group received IVIG within the fourth day of illness(n=34) and the control group received IVIG after the fifth day of illness(n=53). Clinical manifestations, fever duration, hospitalization days, CBC, blood chemistry and coronary artery complications were compared between two groups. Results : No demographic differences were noted between the two groups(P>0.05). Total duration of fever was significantly shorter in the early treatment group than the control group($4.8{\pm}2.5days$ vs $7.4{\pm}3.0days$, P<0.05), but there were no differences in fever duration after IVIG treatment and hospitalization days between two groups(P>0.05). No significant differences were noted in the level of hemoglobin, WBC, ESR, CRP, AST, ALT and albumin between two groups(P>0.05). No significant differences in the incidence of IVIG retreatment were noted between the two groups(11.8% vs 5.7%, P>0.05). No significant differences in the incidence of coronary artery complications were noted between the two groups(11.7% vs 18.9%, P>0.05). No significant differences in the recurrence rate were noted between the two groups(3% vs 2%, P>0.05). Conclusion : Early IVIG treatment in patients with Kawasaki disease reduces the total fever duration. Coronary artery complications were not increased in patients with early IVIG treatment. 목 적 : 가와사끼병 환아에서 면역글로불린을 조기 투여하였을 때 치료 실패의 빈도와 관상 동맥의 합병증이 증가하는 지를 알아보고자 본 연구를 시행하였다. 방 법 : 2001년 1월부터 2002년 6월 사이에 이화의대 목동병원 소아과에서 가와사끼병으로 치료받은 환아 87명의 입원 및 외래 기록을 후향적으로 조사하였다. 면역글로불린을 발열 4일 이전에 투여받은 환아를 조기투여군(34명), 발열 5일 이후에 투여 받은 환아를 대조군(53명)으로 하였다. 두 군간의 임상양상, 혈액 검사, 발열기간, 치료 및 관상 동맥 합병증을 비교 조사하였다. 결 과 : 1) 두 군간의 인구학적 특징의 차이는 없었다(P>0.05). 2) 총 발열기간은 조기 투여군과 대조군이 각각 $4.8{\pm}2.5$일과 $7.4{\pm}3.0$일로 조기 투여군에서 유의하게 짧았다(P<0.05). 면역글로불린 투여 후 발열 기간과 입원기간은 두 군에서 유의한 차이를 보이지 않았다(P>0.05). 3) 혈색소, 백혈구수, 혈소판수, 혈침속도, CRP, AST, ALT, 알부민은 두 군간에 유의한 차이가 없었다(P>0.05). 4) 면역글로불린 재투여 빈도는 조기 투여군과 대조군에서 각각 11.8%와 5.7%로 유의한 차이를 보이지 않았다(P>0.05). 5) 관상 동맥 합병증은 조기 투여군에서 11.7%와 대조군에서 18.9%로 유의한 차이가 없었다(P>0.05). 6) 가와사끼병의 재발은 각각 3%와 2%로 두 군간에 유의한 차이가 없었다(P>0.05). 결 론 : 가와사끼병에서 면역글로불린 조기투여는 총 발열기간을 감소시키고, 면역글로불린 치료 실패율과 관상 동맥류 발생을 증가시키지 않았다.