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국연지,이대훈,송정은,Nirmalya Tripathy,전유신,전하얀,Joaquim M. Oliveira,Rui L. Reis,강길선 한국생체재료학회 2017 생체재료학회지 Vol.21 No.1
Background: The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone. Methods: The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(−)DC and Dex(−)-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(−)DC, Dex(−)-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR). Results: In this study, we assessed osteogenic differentiation of BMSCs on Duck’s feet-derived collagen (DC)/HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days. Conclusion: From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications.
Potency of silk/β-tricalcium phosphate scaffolds as biomaterial for bone repair
이대훈,차재근,신재훈,국연지,전유신,송정은,박찬흠,강길선 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Silk fibroin is a biomaterial for biomedical applications because it has a number of attractive properties such as biocompatibility and biodegradability. However, it has a lack of mechanical properties for bone replacement graft material. To increase osteoconductivity and osteoinductivity, mechanical property and osteoconductivity of silk scaffold were modified by β-tricalcium phosphate (β-TCP). β-TCP as a type of bioceramics has degradable, osteoconductive and minimal cytotoxicity. Physical and chemical characterizations of silk/β-TCP scaffolds were determined by SEM, compressive strength, and FT-IR, etc. Also, osteogenic differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs) cultured in scaffold was assessed using alkaline phosphatase (ALP), MTT, RT-PCR, and SEM. These results showed that silk/β-TCP (75:25) scaffold was higher cell proliferation and differentiation of rBMSCs compared with other groups.