원숭이 외측슬상체배측핵에서 칼슘결합단백 Parvalbumin과 Calbindin-D 28K의 분포

고승희,배춘상,박성식,Ko, Seung-Hee,Bae, Choon-Sang,Park, Sung-Sik 한국현미경학회 1994 Applied microscopy Vol.24 No.4

The calcium-binding proteins (CaBP), parvalbumin (PV) and calbindin-D 28K (calbindin) are particularly abundant and specific in their distribution, and present in different subsets of neurons in many brain regions. Although their physiological roles in the neurons have not been elucidated, they are valuable markers of neuronal subpopulations for anatomical and developmental studies. This study is designed to characterize dorsal lateral geniculate nucleus (dLGN) neurons and axon terminals in terms of differential expression of immunoreactivity (IR) for two well-known CaBPs, PV and calbindin. The experiments were carried out on 6 adult monkeys. Monkeys were perfused under deep Nembutal anesthesia with 2% paraformaldehyde and 0.2% glutaraldehyde in 0.1M phosphate buffer. After removal, the brains were postfixed for 6-8 hr in 2% paraformaldehyde at $4^{\circ}C$ and infiltrated with 30% sucrose at $4^{\circ}C$. Thereafter, they were frozen in dry ice. Serial sections of the thalamus, at $20{\mu}m$, were made in the frontal plane with a sliding microtome. The sections were stained for PV and calbindin with indirect immunocytochemical methods. For electron microscopy, after infiltration with 30% sucrose the blocks of thalamus were serially sectioned at $50{\mu}m$ with a Vibratome in the coronal plane and stained immediately by indirect ABC methods without Triton X-100 in incubation medium. Stained sections were postfixed in 0.2% osmium tetroxide, dehydrated and flat-embedded in Spurr resin. The block was then trimmed to contain only a selected lamina or interlaminar space. The dLGN proper showed strong PV IR in fibers in all laminae and interlaminar zones. Particularly dense staining was noted in layers 1 and 2 that contain many stained fibers from optic tract. Neuronal cell body stained with PV was concentrated only in the laminae. In these laminae staining was moderate in cell bodies of all large and medium-sized neurons, and was strong in cell bodies of some small neurons together with their processes. Calbindin IR was marked in the neuronal cell body and neuropil in the S layers and interlaminar zones whereas moderate in the neuropil throughout the nucleus. Regional difference in distribution of PV and calbindin IR cell is distinct; the former is only in the laminae and the latter in both the S layer and interlaminar space. The CaBP-IR elements were confined to about $10{\mu}m$ in depth of Vibratome section. The IR product for CaBP was mainly associated with synaptic vesicle, pre- and post-synaptic membrane, and outer mitochondrial membrane and along microtubule. PV-IR was noted in various neuronal elements such as neuronal soma, dendrite, RLP, F, PSD and some myelinated or unmyelinated axons, and was not seen in the RSD and glial cells. Only a few neuronal components in dLGN was IR for calbindin and its reaction product was less dense than that of PV, and scattered throughout cytoplasm of soma of some relay neurons, and was also persent in some dendrite, myelinated axons and RLP. The RSD, F, PSD and glial elements were always non-IR for calbindin. Calbindin labelled RLP were presynaptic to unlabeled dendrite or dendritic spine and PSD. Calbindin-labeled dendrite of various sizes were always postsynaptic to unlabeled RSD, RLP or F. From this study it is suggested that dLGN cells of different functional systems and their differential projection to the visual cortex can be distinguished by differential expression of PV and calbindin.

골 - 슬개건 - 골을 이용한 후방십자인대 재건술후 슬관절의 후방 불안정성

고승희,윤선용,류상욱,최철헌 ( Seung Hee Ko,Sun Young Yoon,Sang Wook Rhyu,Chul Hun Choi ) 대한슬관절학회 1997 대한슬관절학회지 Vol.9 No.2

The posterior cruciate ligament is an anatomically and biomechanically complex structure. PCL injuries are reported to occur in 1-40% of acute knee injuries, with isolated PCL tear, less common than PCL tears combined with other Iigament injuries. Controversy exists concerning the geatment of the PCL injures. Recently, arthroscopic techniques of PCL reconstruction are becoming nore refined and reproducible. Between July 1993 and May 1995, 25 operations for PCL rupture were performed in our hospital. At the follow-up examinations we noted mild to moderate posterior instablilty which was not noted at the time of operation and during the immediate postoperative period. We reviewed type (if injuries, amplitude of initial posterior tibia1 translation, surgical techniques, which seemcd to be in relation ivith the postoperative instabilities. At final follow-up, the mean Lysholm knee score was 86 points, and the posterior tibial translation 6.5mm on posterior stress radiographs. The ligament augmentation device provided no benefit. The ideal positioning and fixation of the graft and protection of the graft from abrasion seemed to be important to get a good results.

18~19세기 北關開市의 운영과 성격

高丞嬉(Ko Seung-Hee) 한국사연구회 2000 한국사연구 Vol.109 No.-

조선 후기 북관개시 (북관개시) 연구

고승희 ( Seung Hee Ko ) 조선시대사학회 1997 朝鮮時代史學報 Vol.1 No.-

영상 매칭 기반 실내 장소 안내 모바일 어플리케이션

고승희(Seung Hee Ko),양미현(Mi Hyun Yang),오승현(Seung Hyeon Oh),홍성호(Sung Ho Hong),황성수(Sung Soo Hwang) 한국멀티미디어학회 2016 멀티미디어학회논문지 Vol.19 No.8

A person often needs to visit a facility that have not been visited before. In this case, it is necessary to easily identify the building in which the facility is located. Indoor navigation is also important, as the person may get lost due to the complex structure of buildings. To solve these problems, this paper presents a mobile application which provides a user with indoor place information and indoor navigation. A user is requested to take a photograph of a building of interest or of the current location. Then, the proposed application identifies the building of interest or the current location of the user by utilizing an image matching technique. For fast matching, database filtering by using GPS/magnetometer data and pre-extraction of features for images in the database are utilized. In case of indoor navigation, the application provides the actual images of check points as well as the moving direction. Simulation results show that the proposed system successfully identifies the location of users and provides place information and indoor navigation.

지방자치단체 세외수입 확충을 위한 요율 현실화 방안에 관한 연구 - 천안시 사용료를 중심으로 -

고승희(Seung-Hee Ko),주운현(Woon-Hyun Choo) 한국지방행정학회 2016 한국지방행정학보 (KLAR) Vol.13 No.2

User charges is more efficient means of financing local governments by coinciding the consumers(beneficiaries) and the payers of the public services. However, the amount of the revenue is less than two percent of the total revenue and the level of user fees compared to the cost is differed by regions, agencies, and service types. Therefore, this study aims at providing the basis for estimating appropriate rates of user charges by reviewing non-tax revenues managed by Cheonan and undertaking an analysis on user charges utilizing the pricing method where appropriate. The results of the analysis using pricing method shows that cost recovery rates are about 40%. Regarding reasonable adjustments of rates, the following are suggested. Firstly, it is essential to select an appropriate pricing method, construct the relevant data base and manage the revenues and costs in accordance with the standard of the pricing method chosen. Secondly, regular conducts of pricing method needs be institutionalized. Thidly, it is suggested to examine thoroughly the passivity of the introduction of an ordinance for collection of integrated tax while operating a team for the management of non-tax revenues and establishing a long term strategic plan for the management of non-tax revenue.

지방공기업 경영평가의 수용성 분석

고승희(Seung-Hee Ko) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.12

본 연구는 충청남도 직영기업을 대상으로 지방공기업 경영평가의 수용성을 분석하였다. 이를 위해 지방공기업 경영평가와 관련하여 5개 측면으로 영역을 구성하고 지방공기업 경영평가의 수용성과의 영향여부를 분석하였다. 직영기업은 조직 구조상 다른 공기업들과 다른 형태를 지니고 있어서 경영평가가 효율적으로 운영되기 위해서는 보다 차별화된 전략이 필요시 된다. 상하수도의 경우 지방자치단체 조직으로 경영평가 결과의 환류가 어려운 특징이 있어 관리자의 관심과 노력이 필수적으로 요구된다. 또한, 경영평가를 통한 직영기업의 성과를 높이기 위해서는 담당자의 전문성이 필요시 되는 만큼 이를 위한 정책적 운영방안이 필요시 된다. 무엇보다 경영평가 결과에 대한 인센티브가 다른 공기업처럼 의도한 효과를 내기 어려운 구조에 있다. 따라서 평가결과에 대한 보상시스템에 대한 차별화가 필요할 것이다. 본 연구의 한계가 있으나 상대적으로 특성이 다른 상하수도 관계자에 대한 의식조사가 이루어진 만큼 향후 경영평가제도의 운영방향을 모색하는데 기여할 수 있다는 것에 의의를 지닌다. This research analyzed acceptability of local public enterprise management assessment targeting Chungcheongnam-do direct management enterprises. To do this, this research analyzed influence with acceptability of local public enterprise management assessment by composing the area with 5 aspects related to local public enterprise management assessment. More differentiated strategy is required to operate management assessment efficiently, since direct management enterprises have different shape from other public enterprises in respect of organizational structure. In case of water and sewage, it tends to be difficult to return the result of management assessment to the local government organization, so that managers interest and effort is necessarily required. Also, responsible persons professionalism is required to raise achievement of direct management enterprise through management assessment, politic management plan for this is necessary. Above all, the structure of incentive for management assessment result is difficult in making intended effect as other public enterprises. Therefore, differentiation of compensation system for assessment result is required.

모핑 에어포일 형상의 공력특성 실험연구

고승희(Seung-Hee Ko),배재성(Jae-Sung Bae),김학봉(Hark-Bong Kim),노진호(Jin-Ho Roh),안석민(Seok-Min Ahn) 한국항공우주학회 2012 韓國航空宇宙學會誌 Vol.40 No.10

본 연구는 모핑 항공기 날개를 설계/제작하기 위한 선행 연구로서 기본날개단면 및 모핑날개단면에 대한 공력특성을 실험적으로 조사하였다. 이를 위해 Clark-Y형 에어포일을 가진 기본날개, 기계식 플랩을 가진 날개, 모핑플랩을 가진 날개를 제작하여 풍동실험을 수행하였다. 3축 로드셀을 이용하여 날개에 작용하는 양력, 항력 및 피칭모멘트를 측정하였으며, 풍동실험데이터는 Solid Blockage 와 Wake Blockage를 고려하여 보정하였다. 풍동실험은 각 날개별로 다양한 속도, 레이놀즈, 받음각에 대해 수행되었다. 실험결과는 모핑에어포일의 양력-항력 및 양력 피칭모멘트 특성이 기계식 플랩을 가지는 에어포일에 비해 우수한 것을 보여준다. The present paper is the preliminary study of the development of a morphing aircraft wing and investigates experimently the aerodynamic characteristics of a base airfoil and a morphing airfoil. The wind tunnel tests are conducted for a base Clark-Y airfoil, an airfoil with a mechanical flap, and a morphing airfoil. Lifts, drags, and pitching moments are measured by using a three-axis load cell and they are calibrated by considering solid blockage and wake blockage. The wind tunnel tests are conducted for various air speeds, Reynolds’ numbers, and angles of attack. The experimental results show that the aerodynamic characteristics of the morphing airfoil in lift-drag and lift-pitching moment are better than those of the airfoil with a mechanical flap.

충남도민의 자치의식 조사의 함의

고승희(Ko Seung-Hee) 한국콘텐츠학회 2006 한국콘텐츠학회 종합학술대회 논문집 Vol.4 No.2

민선지방자치 실시 이후 충남도민의 자치의식 조사를 통해 자치의식 제고 및 자치역량 강화와 관련된 정책적 시사점 도출을 통하여 향후 바람직한 충남도민의 자치의식 정립 및 자치역량 강화에 요구되는 일련의 지침을 제공하고자 한다. 이러한 문제하에 자치의식 조사 결과를 통하여 본 정책적 함의는 지방자치단체의 정책능력 신장, 지방의회의 역할과 지방의원의 자질 향상, 지방공무원의 역할향상 기대, 주민의 자치의식과 주민참여 활성화 노력 등으로 요약할 수 있다.

지역주민의 참여의식분석과 정책방향

고승희(Seung-Hee Ko) 한국콘텐츠학회 2015 한국콘텐츠학회논문지 Vol.15 No.12

본 연구는 지역주민들의 지방자치에 대한 인식과 더불어 지방자치 주체들의 참여와 관련한 기본적 기능에 대한 인식, 그리고 주민들의 참여인식과 의지를 분석하고 주민참여를 위한 정책방향을 찾고자 하였다. 분석결과 지방자치에 대한 인식과 주민참여제도 및 의견수렴에 있어 긍정적인 응답이 낮게 나타나고 있었다. 주민들의 참여는 정책의 갈등을 줄이고 성과를 높일 수 있으며 주민들에게는 지역에 대한 관심과 개인적 정치참여를 통한 행복감을 높여 줄 수 있다. 우리나라의 지방자치가 지속적이고 안정적으로 성장할 수 있도록 주민참여에 대한 인식전환에서부터 효율적 제도운영까지 다양한 문제들에 대하여 재점검과 개선의 노력이 요구된다. This study aimed not only to analyze local residents awareness of local government, basic functions related to local government main agents participation, and residents consciousness/will of participation, but also to seek for the policy direction for the residents participation. In the results of the analysis, the positive responses to the awareness of local government, residents participation system, and the collection of opinions were low. The residents participation can reduce the conflict of policies and increase the outcomes while it can also increase the residents happiness through their interest in the region and personal participation in politics. In order to continuously and stably develop the local self-governing system in Korea, it is required to put efforts into reviewing and improving various issues like the change in the awareness of residents participation and efficient management of the system.