단백질흡착 감소용 하이드로겔의 개발 및 인공수정체로의 적용

고나영,이현미,Ko, Na Young,Lee, Hyun Mee 대한화학회 2018 대한화학회지 Vol.62 No.1

인공수정체로 사용되고 있는 소수성 아크릴 하이드로겔의 단백질 흡착 감소와 기능을 향상시키기 위한 하이드로겔을 제조하여 인공수정체로의 응용성을 살펴보고자 한다. 소수성 아크릴 인공수정체는 ethyleneglycol phenyletheracrylate(EGPEA), Styrene, 2-hydroxyethyl methacrylate(HEMA)를 사용하여 제작하였고 첨가제로 polyvinyl pyrrolidone(PVP)와 2-methacryloyloxyethyl phosphorylcholine(MPC)를 사용하였다. 인공수정체의 물리적 특성을 평가하기 위해 함수율, 습윤성, 광투과율 및 단백질 흡착량을 측정하였다. 첨가제를 넣은 모든 시료의 함수율과 습윤성은 증가하였고 단백질 흡착량은 감소하였다. 특히 MPC가 포함된 시료의 단백질 흡착량은 더욱 감소하였다. PVP와 MPC를 첨가한 소수성 아크릴 하이드로겔은 기본적인 물리적 특성을 가지면서 단백질 흡착을 감소시킴으로써 기능이 향상된 인공수정체로의 응용이 가능하다는 것을 확인하였다. The purpose of this study was to decrease the protein adsorption and improve the function of the hydrophobic acrylic Intraocular lens(IOL). Hydrophobic acrylic intraocular lenses were prepared by using ethyleneglycol phenyletheracrylate (EGPEA), styrene and 2-hydroxyethyl methacrylate (HEMA). Polyvinyl pyrrolidone (PVP) and 2-methacryloyloxyethyl phosphorylcholine (MPC) were used as additives. Water contents, wettability, light transmittance and protein adsorption amount were measured to evaluate the physical properties of the intraocular lens. The water content and wettability of all samples containing additives were increased and the amount of protein adsorption decreased. In particular, samples containing MPC showed a further decrease in protein adsorption. The hydrophobic acrylic intraocular lens with PVP and MPC was found to improve the function of the intraocular lens by reducing the protein adsorption while having basic physical properties.

Study on Characteristic Changes of Contact Lenses According to Interpenetrating Polymer Network Time and Method Using Seaweed Polysaccharide

Na-Young Ko(고나영),Pil Heon Lee(이필헌),A-Young Sung(성아영),Hyun Mee Lee(이현미) 한국고분자학회 2021 폴리머 Vol.45 No.5

본 연구는 하이드로겔 콘택트렌즈에 알지네이트와 카라기난과 같은 해조다당류를 상호침투 고분자 네트워크를 진행하여 interpenetrating polymer network(IPN) 시간에 따른 콘택트렌즈의 특성 변화를 살펴보았다. IPN은 알지네이트와 카라기난을 이용하여 semi-IPN과 full-IPN으로 4주 동안 진행하였다. 콘택트렌즈의 기능은 함수율, 산소투과율, 접촉각, 단백질 흡착, 그리고 항균성 등을 측정하여 평가하였다. IPN 시간이 증가함에 따라 콘택트렌즈의 모든 물리·화학적 성질이 향상되었다. 산소과율과 습윤성은 카라기난보다 알지네이트를 사용했을 때가 더 높았다. 함수율과 산소투과율은 semi-IPN 방법이 full-IPN 방법보다 좋았으며 습윤성은 full-IPN 방법이 더 많이 향상되었다. 해조다당류는 하이드로겔 콘택트렌즈의 기능 향상에 도움이 되며 IPN 시간이 길수록 성능이 더욱 증가함을 확인하였다. We investigated the change in the characteristics of contact lenses according to the interpenetrating polymer network (IPN) time and method when a contact lens was dual-networked with alginate and carrageenan. The IPN was formed for four weeks using semi-IPN and full-IPN. The functions of contact lenses were assessed based on the measurement of water content, oxygen permeability, contact angle, protein adsorption, and antimicrobial. With the increase in IPN time, all physical and chemical properties of contact lenses were improved. The oxygen permeability and wettability were better when using alginate than using carrageenan. The water content and oxygen permeability were better when using the semi-IPN method than using the full-IPN method, whereas wettability was further improved when using the full-IPN method. The result of this study verified that seaweed polysaccharides were helpful in the functional improvement of hydrogel contact lenses, and performance was better in a longer IPN time.

피부 질환의 병리조직학적 진단에 대한 통계적 고찰

고나영 ( Na Young Ko ),김수남 ( Soo Nam Kim ) 대한피부과학회 2006 大韓皮膚科學會誌 Vol.44 No.8

Background: Skin biopsy is an effective diagnostic tool commonly used in dermatology practice. Clinicopathologic correlation is important for diagnostic accuracy. Objective: The purpose of this study was to investigate the distribution of skin biopsy specimens, and compare the pathologic diagnosis with the final dermatologic diagnosis. Methods: This study included 4,018 skin biopsy specimens taken during a period of 10 years from June 1995 to May 2005 at the Department of Dermatology, Korea University Anam Hospital. Results & Conclusion: Of all biopsy specimens, 61.49% were diagnosed as tumors, and 38.51% were diagnosed as non-tumors. For the tumors, 87.15% were benign, 4.23% were premalignant and 8.62% were malignant. The rate of concordance between pathologic diagnosis and final dermatologic diagnosis was 76.69%, and the rate of discordance between them was 4.18%. The rate of description without a pathologic diagnosis in pathologic reports was 18.94%. The rate of concordance was higher in tumors (89.65%) than in non-tumors (56.00%), and the rate of description in pathologic reports was higher in non-tumors (39.08%) than in tumors (6.32%). There was no significant difference in the rates of discordance between tumors and non-tumors. The rate of concordance was higher in malignant tumors (95.72%) than in benign tumors (89.77%) or premalignant tumors (74.76%). The rate of description in pathologic reports was higher in premalignant tumors (18.45%) than in benign tumors (6.27%) or malignant tumors (0.95%). There was no significant difference in the rates of discordance between the three groups. (Korean J Dermatol 2006;44(8):914~924)

Effect of Ionic Natural Polysaccharides on the Functional Enhancement of Porous Hydrogel Contact Lenses

Na-Young Ko(고나영),Hyun Mee Lee(이현미) 한국고분자학회 2020 폴리머 Vol.44 No.5

다공성 수화젤 콘택트렌즈를 제작한 후 이온성 천연다당류를 가교시켜 이온성 천연다당류가 콘택트렌즈의 물리적 · 화학적 성질에 미치는 영향을 알아보고자 하였다. 다공성 수화젤은 발포제로서 sodium carbonate를 사용하였으며 이온성 천연다당류로서 alginate, chitosan, agarose를 사용하였다. 다공성 수화젤은 일반 수화젤보다 함수율, 산소투과율, 습윤성이 매우 향상되었다. 천연다당류가 결합된 콘택트렌즈는 이온성의 종류에 관계없이 모든 다공성 콘택트렌즈에서 습윤성과 항균성을 향상시켰다. 콘택트렌즈의 단백질 흡착은 다당류의 이온성에 영향을 받았다. 이온성 천연다당류가 수화젤 콘택트렌즈에 결합됨으로써 콘택트렌즈의 기능향상에 기여함을 확인하였다. The purpose of this study was to examine the effect of ionic natural polysaccharides on the physico-chemistry properties of contact lenses by cross-linking ionic natural polysaccharides after fabricating porous hydrogel contact lenses. Sodium carbonate was used as a foaming agent in the preparation of porous hydrogels, and ionic natural polysaccharides such as alginate, chitosan, and agarose were used. Porous hydrogels have significantly improved water content, oxygen permeability, and wettability than ordinary hydrogels (polymacon). Natural polysaccharide cross-linked contact lenses have improved wettability and antibacterial properties in all porous contact lenses, regardless of their ionic type. Protein adsorption of contact lenses was affected by the ionicity of polysaccharides. It was confirmed that the ionic natural polysaccharide contributes to the improvement of the function of the contact lens by being bound to the hydrogel contact lens.

림프관 확장증형 모기질종 3예

고나영 ( Na Young Ko ),조현민 ( Hyun Min Cho ),서수홍 ( Soo Hong Seo ),안효현 ( Hyo Hyen Ahn ),계영철 ( Young Chul Kye ),김수남 ( Soo Nam Kim ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.3

Roles of Src-family kinase isoforms, Lyn, Fyn, Fgr, and c-Src on degranulation in RBL-2H3 mast cells

이준호,문세환,고나영,김지완,김도균,김주동,허억,최완수,Lee, Jun-Ho,Mun, Se-Hwan,Ko, Na-Young,Kim, Jie-Wan,Kim, Do-Kyun,Kim, Joo-Dong,Her, Erk,Choi, Wahn-Soo Korean Society of Life Science 2007 생명과학회지 Vol.17 No.3

흰쥐유래의 비만세포인 RBL-2H3 세포는 다양한 Src-family kinase를 발현한다. 현재까지의 연구결과에 의하면 비만세포의 초기 활성화에 Lyn kinase가 중요한 역할을 한다고 알려져 왔다. 그러나 그 세포에서 발현되는 다양한 다른 Src-family kinase의 역할은 불분명하다. 본 연구에서는 비만세포에서 다양한 Src-family kinase가 세포내 다른 곳에서 다양하게 발현되고 있다는 사실을 RT-PCR, immunoblotting 그리고 confocal microscopy 기법을 이용하여 증명하였다. 그 결과 Lyn 및 Fgr kinase는 세포막에 위치하고 c-Src 및 Yes kinase는 세포 내 과립에 존재하는 것을 알 수 있었다. 모든 Src-family kinase를 클로닝하고 과발현하여 탈과립 대한 영향을 평가하였다. 그 결과 fyn과 Fgr kinase는 비만세포에서 항원 유도의 탈과립을 증가시켰으며 반면 Lyn kinsae는 탈과립을 억제시키는 것을 확인할 수 있었다. 이러한 결과는 비만세포 초기 신호전달계에서 Fgr가 중요한 역할을 할 가능성을 제시한다. The rat RBL-2H3 mast cells contain various Src-family kinases. Previous reports with this cell line indicated that Lyn activation is an important initial signaling for the activation of the cells. However, the role and location of other Src-family kinase isoforms which are expressed in the cells are not clear. In this study, we now show that isoforms of Src-family kinases, Lyn, fyn, Fgr, c-Src, and Yes are differentially expressed and located differently in the cells as indicated by RT-PCR, immunoblotting analysis, and confocal microscopy. Lyn and Fgr were located on plasma membrane but on the other hand c-Src and Yes were located on intracellular organelle. All of Src-family kinases were cloned and overexpressed for investigating the roles of the isoforms. Overexpression of Fyn and Fgr, not Lyn and c-Src, stimulated Ag-induced degranulation in the cells. Our findings strongly suggest for the first time that each of Src-family kinase isoform can regulate differentially $Fc{\varepsilon}RI$-mediated signaling in RBL-2H3 mast cells.

에크린 한공암종

신재빈 ( Jae Bin Shin ),고나영 ( Na Young Ko ),서수홍 ( Soo Hong Seo ),김애리 ( Ae Ree Kim ),계영철 ( Young Chul Kye ),김수남 ( Soo Nam Kim ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.9

Eccrine porocarcinoma is a very rare, locally-aggressive, potentially fatal tumor arising from the intraepidermal ductal portion of the eccrine sweat gland. It develops either spontaneously or from a long standing benign eccrine poroma. It usually affects older people and is located most commonly on the lower extremities. We report a case of eccrine porocarcinoma which developed on the scalp of an 82-year-old woman. There was no evidence of metastasis, and the tumor was successfully removed by Mohs micrographic surgery. (Korean J Dermatol 2006; 44(9):1084~1087)

상온중합환경에 따른 콘택트렌즈의 물성변화

배주현(Ju-Hyun Bae),고나영(Na Young Ko),이현미(Hyun Mee Lee) 한국안광학회 2019 한국안광학회지 Vol.24 No.3

Purpose: The purpose of this study is to investigate the possibilities of producing contact lenses with better properties than those made produced by using conventional methods, by using a new room-temperature (20~30℃) polymerization method and without conventional thermal polymerization. Methods: The contact lens was prepared by controlling the amounts of catalyst N,N,N,N-tetramethyl-ethylenediamine (TMEDA) volume (3 ~ 9 μl) and temperature (20~30℃), respectively. The same reactants were used for both the thermal polymerization and room-temperature polymerization; the contact lens properties such as transmittance, water content, refractive index, wettability, and oxygen permeability were measured and compared. Results: The transmittance of the contact lens was the highest at 25℃. The contact lens showed good optical properties when 3 μl and 5 μl of catalyst were used. The physical properties of the polymerized lens at room temperature were improved regardless of the amount of catalyst under the same conditions, compared with the thermally polymerized lens. When the amount of TMEDA was 5 μl, the polymerization time was reduced, and physical properties such as water content, oxygen permeability, and wettability were significantly improved. Conclusions: Polymerization conditions with optimum physical properties were obtained by room-temperature polymerization, and the possibility of manufacturing high functionality contact lenses was confirmed.

소년원생의 안정적 사회정착을 위한 실태조사 및 정책지원 방안 연구 (Ⅰ)

박성훈(Park, Seong-hoon),김지영(Kim, Ji-young),조영오(Jo, Young-oh),김현정(Kim, Hyun-jung),황여정(Hwang, Yeo-jung),김정숙(Kim Jung-sook),배상균(Bae, Sang-kyun),고나영(Ko, Na-yeong) 한국형사법무정책연구원 2017 형사정책연구원 연구총서 Vol.- No.-

비전형 지방종성 종양 1예

최명훈 ( Myeung Hun Choi ),최재은 ( Jae Eun Choi ),고나영 ( Na Young Ko ),안효현 ( Hyo Hyen Ahn ),계영철 ( Young Chul Kye ),김수남 ( Soo Nam Kim ) 대한피부과학회 2007 大韓皮膚科學會誌 Vol.45 No.3