裁培왕벚의 推定兩親과 交雜種에 對한 遺傳學的 硏究(Ⅰ) : Particularly on the Genetics of Prunus yedoensis Wildly grown in Mt. Halla 漢拏山 自生왕벚의 遺傳特性

任慶彬,李萬相 圓光大學校 1989 論文集 Vol.23 No.2

栽培왕벚의 起源과 原産地를 좀 더 科學的으로 調査하고저 濟州에 自生하는 왕벚 推定兩親인 올벚과 산벚 그리고 올벚과 산벚의 交雜種, 自生왕벚의 實生樹에 對하여 種의 基準特性, 種間變異, 種內變異 等의 分析, 育種의 基礎的硏究로서 花器의 形質, 開花習性, 開花期, 雌雄配偶體形成, 卵裝置와 열매의 形質 等을 調査하였던 바 그 結果는 다음과 같다. 1. 올벚은 濟州 漢拏山 海拔 450∼800m 附近에, 산벚은 600∼1,400附近에 分布되어 있고, 自生왕벚은 海拔 500∼800m 附近에 分布되어 있다. 2. 形態的 및 生態的特性, 交雜性, 交雜種 또는 實生植物들의 分雜 및 開花期 等으로 보아 自生왕벚은 올벚과 산벚의 種間交雜種으로서 올벚과 산벚의 中間型이다. 3. 自然狀態에서 結實率이 올벚은 89%, 산벚은 16%, 自生왕벚은 4.2%, 栽培왕벚은 4.4%이고, 올벚은 산벚의 人工交雜率은 20%이였다. 4. 生育地나 그해 봄의 氣溫에 따라 올벚의 開花期가 遲延되는데 올벚과 산벚이 同時에 開花되는 때도 있다. 5. 열매와 核은 自生왕벚이 올벚, 산벚보다 크고, 올벚과 산벚의 交雜種 F₁群은 兩親에 比해서 變異幅이 擴大되었다. 6. 大胞子母細胞가 減數分裂이 되어 完全胚囊의 卵裝置形成率은 올벚은 80∼90%, 산벚은 40∼50%, 自生왕벚은 4∼6%, 裁培왕벚은 5∼7%이고, 올벚과 산벚의 交雜種 F?植物은 5∼10%였다. 7. 小胞子母細胞가 減數分裂이 되어 完全한 花粉粒을 형성하는 率은 올벚은 90%, 산벚은 85%, 自生왕벚은 10%, 裁培왕벚은 15%이고, 올벚과 산벚의 交雜種 F?植物群은 13∼18%였다. 8. 濟州에 自生하는 왕벚의 實生植物에서는 산벚에 가까운 個體가 많고, 裁培왕벚의 實生樹들은 올벚에 가까운 個體가 많이 出現하였다. 9. 잎의 各種形質을 比較할때 葉型比等 種間 差異를 뚜렷이 반영하는 것이 있었고, 特히 注目할 것은 統計分析으로 왕벚나무는 올벚나무와 산벚나무 사이에 태어난 雜種이란 證據를 强力하게 제시하였다는 點이다. 그리고, 鋸齒, 側脈, 腺 等으로 種의 基本據點을 分析하고 그 結果를 제시하였다. 10. 以上의 形態的 特性이나 올벚과 산벚의 交雜性 및 F₁植物의 分離比, 自生왕벚의 實生植物과 裁培왕벚의 實生植物들로 보거나, 花器構造, 開花期, 發生學的 特性으로 보아 裁培왕벚은 濟州道 漢拏山에서 올벚과 산벚의 交雜種이라 證明할 수 있다. To elucidate the genetic and habitat origins of Prunus yedoensis var. nudiflora Koehne currently cultivated for the beautifying purpose, the following research materials were used in this study; (1) a naturally grown tree of P. yedoensis var. nudiflora Koehne designated as the natural monument No. 1 in Cheju Island(symbol, p. y. nm) (2) P. subhirtella var. pendula form ascendens(symblo, p. s.) (3)P. donarium(symbol, p. d.), both of which are assumed to be the putative parents for P. yedoensis var. nudiflora Koehne (4) The hybrid F₁ artificially induced through p. s.×p. d.(symbol, p. s.×p. d.) (5) the seedings produced from the open-pollinated seeds of p. y. nm(symbol, p. y. F₁) Various characteristics such as dimensions of various floral parts and leaves, opening and fading-out dates of flowering, formation and development of male and female gametophytes and egg apparatus, meiosis abnormality of megaspore and microspore, fertility and development of fruit, branch habit and tree shape were observed and measured and discussed in connection with the research purposes. The results obtained are summerized as follows; 1. In Cheju Island, the habitat segregation of three Prunus species, p. y., p. d., and p. s. can be clearly recognized. Each distributional areas is confined in relation with altitudes, i. e., 450∼800 meters above sea level for p. s., 600∼1400 meters for p. d., and 500∼800 meters for p. y. P. yedoensis individuals in Cheju Island occur to the area situated between two opposite areas for p. d. and p. s. The distributional patterns bring the possibility of the natural crossing between p. d. and p. s. to produce p. y. as being an interspecies hybrid. 2. Taking considerations of morphological and ecological characteristics, crossability and fertity, the magnitudes of the means and variances of some measurements for the three species mentioned before, the naturally grown P. yedoensis var. nudiflora Koehne comes between P. s. and P. d. 3. In open pollinated condition, the fruting ratios producing viable seeds were 89 percent for P. s., 16 percent for P. d., 4.2 percent for P. y. nm., 4.4 percent for cultivated P. y. and 20 percent for P. s.×P. d. 4. The initiating dates of flowering for the species are variable according to the annual temperature fluctuation. As being a rate situation, however both P. s. and P. d. begin the flowering synchronously. 5. In regard to the sizes of fruit and stone(seed), P. y. nm had bigger ones than those of the other two species. And the variances for P. s.×P. d. F₁ were further extended in compared with other species. 6. The percentages of complete egg apparatus formation followed after the meiosis process of megaspore mother cells were 80∼90 for P. s.,40∼50 for P. d., 4∼6 for P. y. nm, 5∼7 for cultivated P. y. and 5∼10 for P. s.×P. d. F₁ individuals. 7. The percentages of complete pollen grain formation followed after the meiosis process of microspore mother cells were 90 for P. s., 85 for P. d., 10 for P. y. nm., 15 for cultivated P. y. and 13∼18 for P. d., 10 for P. y. nm., 15 for cultivated P. y. and 13∼18 for P. s×P. d. F₁ individuals. 8. The individuals of seed origin propagated from P. y. nm. inclined to produce more P. d.-like individuals, but obtained from cultivated P. y. were P. s.-like individuals. 9. The statistial analysis of leaf dimensims brought firm belief that P. s. and P. d. were the putative parents of P. yeodensis var. nudiflora Koehne grown in Cheju Island.

COLD PERFORMANCE OF VARIOUS BIODIESEL FUEL BLENDS AT LOW TEMPERATURE

J.-K. KIM,E. S. YIM,C. H. JEON,C.-S. JUNG,B. H. HAN 한국자동차공학회 2012 International journal of automotive technology Vol.13 No.2

This study examines the cold performance of biodiesel blends in a passenger car and a light duty truck at -16 oC and -20 oC. Six different types of biodiesels derived from soybean oil, waste cooking oil, rapeseed oil, cottonseed oil, palm oil and jatropha oil were blended with different volume ratios (B5 (5 vol. % biodiesel - 95 vol. % diesel), B10 and B20). The cold filter plugging point (CFPP) and the cloud point had an effect on the startability and driveability of both the passenger car and the light duty truck. The startability and driveability of the passenger car with all biodiesel blends (B5) were generally good at -20 oC. In the light duty truck, biodiesel blends (B10 and B20) of soybean, waste cooking, rapeseed and jatropha tended to be good at -20 oC in the startability and driveability tests than the biodiesel blends (B10 and B20) of cottonseed and palm. In particular, the palm biodiesel blend (B10) failed at -20 oC, and the palm biodiesel blend (B20) also failed at -16 oC in the startability test. The cold flow properties of biodiesel dictate that the length of the hydrocarbon chains and the presence of unsaturated structures significantly affect the low temperature properties of biodiesel.

Plk1-mediated stabilization of 53BP1 through USP7 regulates centrosome positioning to maintain bipolarity

Yim, H,Shin, S-B,Woo, S U,Lee, P C-W,Erikson, R L The Author(s) 2017 Oncogene Vol.36 No.7

<P>Although 53BP1 has been established well as a mediator in DNA damage response, its function in mitosis is not clearly understood. We found that 53BP1 is a mitotic-binding partner of the kinases Plk1 and AuroraA, and that the binding with Plk1 increases the stability of 53BP1 by accelerating its interaction with the deubiquitinase USP7. Depletion of 53BP1 induces mitotic defects such as chromosomal missegregation, misorientation of spindle poles and the generation of extra centrosomes, which is similar phenotype to USP7-knockdown cells. In addition, 53BP1 depletion reduces the levels of p53 and centromere protein F ( CENPF), interacting proteins of 53BP1. These phenotypes induced by 53BP1 depletion were rescued by expression of wild-type or phosphomimic mutant 53BP1 but not by expression of a dephosphomimic mutant. We propose that phosphorylation of 53BP1 at S380 accelerates complex formation with USP7 and CENPF to regulate their stability, thus having a crucial role in proper centrosome positioning, chromosomal alignment, and centrosome number.</P>

Comparison of desorption enhancement methods in the low temperature plasma ionization mass spectrometry for detecting fatty acids in Drosophila

Kim, S.H.,Jang, H.J.,Park, J.H.,Lee, H.J.,Kim, J.,Yim, Y.H.,Kim, D.B.,Yoon, S. Elsevier 2017 Current Applied Physics Vol.17 No.8

<P>Mass spectrometry (MS) with low temperature plasma (LTP) as an ionization source is one of the widely used ambient methods in analyzing various bio-chemical samples for their detection, identification, differentiation, etc. While the LTP-MS allows selective analysis of a sample with low-molecular weight without thermal damages, it has difficulties desorbing a target molecule from the sample surface with a low volatility. Hence, for the purpose of enhancing the desorption and ionization efficiencies of the LTP-MS when analyzing the fatty acids, two methods were compared: directly heating the sample surface and increasing the vapor pressure of the fatty acid itself by derivatization. In addition, the fatty acids in Drosophila were directly analyzed with the LTP-MS. As a result, it was found that the LTP-MS detection efficiency was enhanced with the derivatization as much as with the heating condition while there was little synergy in employing both of the heating and derivatization together. (C) 2017 Elsevier B.V. All rights reserved.</P>

The Variation of natural population of pinus densifiora S. et Z. in Korea

K.B. YIM(任慶彬),Y.S. KIM(金用植),K.J. LEE(李景宰) 한국육종학회 1981 한국육종학회지 Vol.13 No.2

In the geographic variations of the resin canal numbers, tracheid length, and seed length, the natural populations of Pinus densiflora examined could be devided into two groups, one along the Taebek Mountains, and the other one along the Sobek Mountains, two main mountain chains in this country. This grouping could be confirmed by the statistical analysis of T-test. The length of the tracheid and the seed are generally longer in the Sobek group than that in the Taebek group. The epicotyl length showed a strong north to south clinal variation particularly along the Sobek Mountains. The mean seed-wing width and length, seed length, thousand seed weight, cone diameter and length, cone weight, mean width of 10 annual ring segment, specific gravity of wood, early to late wood ratio, resin canal number, and the seedling height showed highly significant differences among populations and some characters within population, and also between families.

A Method for testing seed viability of some coniferous trees in Korea

H. S. Park(朴孝燮),D. K. Lee(李敦求),K. B. Yim(任慶彬) 한국육종학회 1983 한국육종학회지 Vol.15 No.3

This study was conducted to examine the possibility of determining the internal characters of four coniferous tree seeds (Pinus densiflora, Pinus koraiensis, Pinus rigida and Larix leptolepis) by X-ray photography, to examine the influence of seed (embryo-endosperm) quality on germination, and to compare the seed germinability between by tetrazolium method and by X-ray contrast method. X-ray photography was a useful tool to measure the embryo and endosperm development of the conifer seeds. The different stages of embryo and endosperm development observed on the photographic plates were classified into 5 embryo-classes (0, I, II, III and IV) and 2 endosperm-classes(A and B). The seeds of Larix leptolepis and Pines rigida were classified into 4 embryo-classes and 2 endosperm-classes. Pines densiflora seeds mostly consisted of III-A and IV-A classes. Many of Larix leptolepis seeds were empty. The seeds of Pinus densiflora and Pinus rigida exhibited mostly IV-A class and those of Pinus koraiensis mostly III-A class. The degree of embryo-endosperm development affected the germinability. The higher was the embryo-endosperm class the better was the germinability of the seed. The seeds belonged to embryo class II were germinated slower than those to classes III and IV. The germination capacity of endosperm class A was better than that of endosperm class B. The results compared between X-ray contrast and tetrazolium methods for examining germinability indicated that the germination values obtained by X-ray contrast method were more closely related to those in an incubator than to those obtained from the tetrazolium method The tetrazolium method was reliable for the rapid determination of seed germinability if treated properly. However, the X-ray contrast method was more precise, simpler and quicker than tetrazolium method for examining seed germinability. Thus, the X-ray contrast method is recommendable to examine seed viability for these four coniferous species.

인슐린 비의존성 당뇨병 동물모델에서 미토콘드리아 DNA 복제수의 감소

송지현,임선희,김영미,한복기,이홍규,박경수,Suzuki, S. 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Portable, non-destructive tester integrating VIS/NIR reflectance spectroscopy for the detection of sugar content in Asian pears

Choi, J.H.,Chen, P.A.,Lee, B.,Yim, S.H.,Kim, M.S.,Bae, Y.S.,Lim, D.C.,Seo, H.J. International Society for Horticultural Science ; 2017 Scientia horticulturae Vol.220 No.-

<P>In this study, a portable, non-destructive tester, integrating near-infrared (NIR) spectroscopy, was developed to determine the maturity of Asian pears. In addition, regression analysis was conducted for mature Manpungbae pears using the partial least squares regression model with the Brix value of Asian pears in a wavelength range of 710-930 nm, and the standard error of calibration or standard error of prediction (SEC/SEP) of 0.41-0.43 degrees Brix and relative standard error of calibration or relative standard error of prediction (rSEC/rSEP) = 0.91-0.92 were observed, indicating excellent prediction performance with no dependence on pretreatment conditions. Furthermore, the reliability of the developed Brix determination model was confirmed using Manpungbae, Wonhwang, Chuhwangbae, and Niitaka pears, and SEP, rSEP, and bias values of 0.29-0.33 degrees Brix, 0.90-0.96, and 1.67E-04, respectively, were observed, indicating high reliability. Hence, the construction of a Brix determination model by the accumulation of absorbance for several varieties of pears in each harvest period is expected to determine optimum harvest times via the measurement of the Brix values at full maturity regardless, of the variety. (C) 2017 Elsevier B.V. All rights reserved.</P>

뇌병변장애인의 일상생활을 위한 맞춤형 보조기구의 설계․제작․적용 사례 연구

이진현(J. H. Lee),최미나(M. N. Choi),임성빈(S. B. Yim),이근민(G. M. Rhee) 한국재활복지공학회 2010 재활복지공학회논문지 Vol.4 No.1

본 논문은 뇌병변장애인의 일상생활을 위한 맞춤형 보조기구 설계?제작?적용 사례연구로 대구광역시보조기구센터 장애인 보조기구 사례관리 시범사업의 2010년 일상생활영역의 맞춤 서비스 사례 중 2명을 대상으로 2010년 10월 18일부터 11월 17일까지 1개월간 진행되었다. 대상자 A는 47세 뇌병변장애인으로 양 하지의 근력과 기능이 많이 저하되어 바닥에서만 생활하며 스스로 변기에 올라 앉아 배변활동을 할 수 없었다. 이에 대상자 A에게 이동식 좌변기와 맞춤 경사로를 설계??제작하여 상지의 잔존근력으로 경사로를 올라가서 이동식 좌변기에 스스로 앉아 배변활동을 할 수 있도록 적용하였다. 대상자 B는 8세 뇌병변 장애인으로 손 기능이 저하되어 스스로 식사를 할 수 없었다. 기존에 나와 있는 여러 가지 식사보조도구들을 1차 적용하였으나 대상자에게 적합하지 않아 대상자의 관절가동범위(ROM)와 손 크기를 고려하여 맞춤으로 식사보조도구를 설계?제작하였고, 작업치료와 연계하여 지속적인 훈련을 통해 향후 스스로 식사를 할 수 있도록 적용하였다. 대상자 A와 대상자 B는 기존에 나와 있는 제품의 적용이나 개조가 접근할 수 없는 사례였음으로 대상자에게 맞춤형으로 설계?제작되어 적용되었다. 본 사례연구를 통해 맞춤형 보조도구의 설계?제작 서비스의 대한 중요성과 필요성을 인식할 수 있다. This paper is case study on design, manufacture and application of customized assistive device for the ADL of person with brain lesions. The study had carried out from October 2010 to November 2010 and 2 clients from Assistive device case management demonstration project which is performed by Daegu assistive and rehabilitation center had participated. Case A, a 47-year-old man with brain lesions, stays only on the floor and could not get close to the usual toilet by himself because of the poor low extremities muscle strength and function. The moveable-toilet and customized ramp had applied to Case A. He clambers up the ramp and reach to the toilet easily. Case B, a 8-year-old boy with brain lesions, had difficulties with self-feeding because of his clumsy hand. Several existing feeding aids were applied but not appropriated. A customized feeding aid was designed and manufactured out of regard of Case A/s ROM and right hand size. And his occupational therapist works together continuously to make him feed himself in the near future. Already-made assistive devices were not appropriate to Case A and B and to solve this problem, customized assistive devices were applied. The importance and needs of design and manufacture of assistive device were recognized through these case study.

韓紙를 利用한 graphite paper에 關한 硏究

任洪彬,李輔成 충남대학교 공업기술개발연구소 1975 工業技術開發硏究所論文集 Vol.2 No.1

This study was concerned about the carbonizing and graphitizing of papery fibre. As pure paper fibre was Korean Paper, Mulberry Paper, applied to the research. Conditions of the carbonizing and graphitizing were determined through the thermal gravimetry and the differential thermal analysis and with this condition was graphite paper successfuly produced. This study was concerned too about the composite reinforced with graphite paper. Physical properties of the graphite paper and of the composite were defined and compared with others. It found that the advantage of the composite with graphite paper stood over the others in manufacturing of plate composite and the strength of this composite was able to compete with others.