분자역학적 방법을 이용한 부산시내 대형건물 냉각탑수의 레지오넬라균의 형별 분류

김정만,정석훈,서대영,박은희,송은주,최재철,이은엽,장철훈 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

숫돌 형상 변화에 따른 연삭가공면 특성

왕덕현,김원일,강신엽,우정윤 경남대학교 신소재연구소 1997 論文集 Vol.9 No.-

숫돌 형상 변화에 따른 연삭가공시 나타나는 온도의 변화를 연삭가공면의 특성으로 파악하는 실험적 연구를 하였다. 숫돌의 형상은 슬롯형태로 폭과 각도의 변화에 의하여 변경시켰으며, 또한 일반숫돌인 경우 특성을 비교 분석하였다. 슬롯형 숫돌인 경우 슬롯의 폭이 10mm이고, 각도가 30°때 낮은 연삭온도를 나타내고 있었다. 표면거칠기 상태는 일반숫돌인 경우보다 슬롯형 숫돌일 경우 대체로 표면상태가 나쁘다는 것을 알았으며, 슬롯의 폭이 6mm이고 각도가 45°인 숫돌과 일반숫돌의 특성을 비교해 볼 때 유사한 경향을 나타내고 있었다. The characteristics of ground surface by different shapes of wheel were found by grinding temperature and surface roughness. The shapes of wheel are changed by slots, which has different width and helical angle, and compared with the case of general one. Lower grinding temperature was obtained for 30°helical angle and width of 10mm. The characteristics of surface roughness of slotted wheels were found to be rough, but the value of roughness for the slotted wheel with 45°helical angel and width of 6mm was similar tendency for the general wheel.

혈압측정의 정확성 평가

조성현,황정해,김은경,오병희,김창엽 한국의료QA학회 1996 한국의료질향상학회지 Vol.3 No.1

Background : Blood pressure is an important indicator in diagnosis and assessing treatment of a patient. Clinical staffs use blood pressure on the assumption that measured value is accurate and reliable. However, whether measured blood pressure is accurate has been rarely investigated in Korea. Objectives : The aims of this study are to evaluate clinical staffs' knowledge and technique as well as accuracy of sphygmomanometer. Also the program to improve the measurement is developed. Methods : Seventy-three registered nurses were asked nine multiple choice questions including Korotkoff sound, cuff size, and deflation rate. Simultaneously characteristics of nurses were examined age, working place, duration of employment and academic degree. A testing videotape(Standardizing Measurement Video-Tutored Course) was used for evaluating the accuracy of measurement. Testees were to read and record the 12 cases of blood pressure measurement, watching a falling mercury column and hearing Korotkoff sounds. After 10 minutes' education, they were again tested with the same cases. Additionally, 83 mercury sphygmomanometers were checked to find defects such as inaccurate calibration and zero setting, leaky bladder, etc. Results : For the knowledge testing correct response rate was 41.1%. They were the lowest in selecting the proper cuff size and Korotkoff sound. In examining accuracy of blood pressure with videotape, nurses had 67.7% correct response rate. The correct response rate was significantly improved by a session of education. About 23% of sphygmomanometers was without discemable defects. Conclusion : The knowledge and skill of clinical staffs along with the accuracy of equipment have to be improved. A properly designed education program would contribute to the accuracy improvement of blood pressure measurement. Also, more concerns should be given to the precision and maintenance of equipment.

심인성 어지럼증 환자의 정신과적 증상에 관한 연구

이경규,이지영,김현우,이정엽,백기청,김재일,이근호 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

연구목적 : 어지럼증을 호소하는 환자들 중에 심인성 어지럼증 환자가 상당수 있다는 것은 알려져 있고, 이러한 심인성 어지럼증과 동반되는 정신질환으로는 불안장애, 우울장애, 인격장애 등이 있다. 이에 본 연구에서는 심인성 어지럼증 환자들의 빈도와 정신과적 증상을 알아보고자 하였다. 방 법 : 어지럼증 환자들에게 평형검사, 자세운동측정검사, 자세변화검사, caloric testing을 시행하여 심인성, 말초성 및 중추성의 3군으로 분류한 후 각각 14명, 16명, 32명을 대상으로 하였다. 대상환자들에게 한국판 Beck 우울척도검사(K-BDI), Spielberg의 상태-특성 불안검사(STAI). 간이정신진단검사(SCL-90-R)를 이용하여 정신과적 증상을 측정하여 이들 세 군을 비교분석하였다. 결 과 : 1) 심인성 어지럼증을 가진 환자는 어지럼증을 나타내는 환자 총 62명중에서 14명으로 22.6%를 차지하였다. 2) K-BDI에 따른 세 군간의 비교에서는 세 군간에 유의한 차이를 보이지 않았다. 3) STAI에 따른 세 군간의 비교에서 상태불안(STAI-S) 및 특성불안(STAI-T) 모두에서 세 군간에 유의한 차이를 보이지 않았다. 4) SCL-90-R에 따른 세 군간의 비교에서는 9개의 척도 중에서 공포불안척도에서만 중추성 어지럼증군이 47.56±6.90, 말초성 어지럼증 군이 53.50±13.74, 심인성 어지럼증 군이 58.50±16.05로 심인성 어지럼증 환자군이 중추성 어지럼증 환자군에 비하여 유의하게 높은 것으로 나타났다(p<0.01). 5) 통증 증상에 있어서는 세 군간에 유의한 차이를 보이지 않았고, 위장관 증상과 성기능 장애 증상은 중추성과 말초성 어지럼증 군이 심인성 어지럼증 군에 비하여 유의하게 높은 것으로 나타났다(p<0.01). 결 론 : 상기 결과를 토대로 했을 때 어지럼증 환자들에 대한 정신과적 접근을 위하여 각 군을 나누는 것은 별 의미가 없으며 어지럼증 환자들의 어지럼증 자체에 대한 직접적이고 집중적인 치료가 더욱 필요할 것으로 생각된다. Objectives : This study was aimed to investigate psychiatric symptoms in patients with psychogenic dizziness and compare these findings with those of patients with central and peripheral dizziness. Methods : A total of 62 patients with dizziness was the subject of investigation, and patients were classified into 32 with central type, 16 with peripheral type, and 14 with psychogenic type. Korean standardized Beck Depression Inventory(K-BDI), State and Trait Anxiety Inventory(STAI), and Korean standardized edition of Symptom Checklist 90 Revised(SCL-90-R) were used for the assessment. Statistically, Pearson's chi-square test and one-way ANOVA with Scheffe's test were used with SPSS/PC for windows 6.0. Results : The results were as follows : 1) The proportion of psychogenic dizziness was 22.6% of the total subjects. 2) Total scores of K-BDI were not significantly different among the 3 groups. 3) Total scores of state anxiety and trait anxiety were not significantly different among the 3 groups. 4) In SCL-90-R, psychogenic dizziness group showed significantly higher score of phobic anxiety only(p<0.01) than central dizziness group. And others were not significantly different among the 3 groups. 5) In additional somatic symptoms, pain score was not different among the 3 groups, but gastrointestinal and sexual symptoms scores of central and peripheral dizziness group were significantly higher than those of psychogenic dizziness group(p<0.01). Conclusion : These results suggest that psychiatric symptoms in patients with psychogenic dizziness are not different from those of patients with central or peripheral dizziness. Therefore, the more direct and intensive treatment may be necessary regardless of the type of dizziness.

Fluorine Functionalized Graphene Nano Platelets for Highly Stable Inverted Perovskite Solar Cells

Kim, Gi-Hwan,Jang, Hyungsu,Yoon, Yung Jin,Jeong, Jaeki,Park, Song Yi,Walker, Bright,Jeon, In-Yup,Jo, Yimhyun,Yoon, Hyun,Kim, Minjin,Baek, Jong-Beom,Kim, Dong Suk,Kim, Jin Young American Chemical Society 2017 NANO LETTERS Vol.17 No.10

<P>Edged-selectively fluorine (F) functionalized graphene nanoplatelets (EFGnPs-F) with a p-i-n structure of perovskite solar cells achieved 82% stability relative to initial performance over 30 days of air exposure without encapsulation. The enhanced stability stems from F substitution on EFGnPs; fluorocarbons such as polytetrafluoroethylene are well-known for their superhydrophobic properties and being impervious to chemical degradation. These hydrophobic moieties tightly protect perovskite layers from air degradation. To directly compare the effect of similar hydrophilic graphene layers, edge-selectively hydrogen functionalized graphene nanoplatelet (EFGnPs-H) treated devices were tested under the same conditions. Like the pristine MAPbI(3) perovskite devices, EFGnPs-H treated devices were completely degraded after 10 days. The hydrophobic properties of EFGnPs-F were characterized by contact angle measurement. The test results showed great water repellency compared to pristine perovskite films or EFGnPs-H coated films. This resulted in highly air-stable p-i-n perovskite solar cells.</P>

The Liquid <i>Panax ginseng</i> Inhibits Epidermal Growth factor-induced metalloproteinase 9 and cyclooxygenase 2 expressions <i>via</i> inhibition of inhibitor factor kappa-B-alpha and extracellular signal-regulated kinase in NCI-H292 human airway epith

Kim, Jung-Hee,Kang, Jeong Woo,Kim, ManSub,Lee, Dong Hun,Kim, Heejong,Choi, Hee-Sook,Kim, Eun Jin,Chung, Ill-Min,Chung, Il-Yup,Yoon, Do-Young SAGE Publications 2011 American journal of rhinology & allergy Vol.25 No.2

Vitamin D deficiency in women with polycystic ovary syndrome

Kim, Jin Ju,Choi, Young Min,Chae, Soo Jin,Hwang, Kyu Ri,Yoon, Sang Ho,Kim, Min Jeong,Kim, Sun Mie,Ku, Seung Yup,Kim, Seok Hyun,Kim, Jung Gu The Korean Society for Reproductive Medicine 2014 Clinical and Experimental Reproductive Medicine Vol.41 No.2

Objective: To investigate: the prevalence of vitamin D deficiency in Korean women with polycystic ovary syndrome (PCOS), and the relationship between vitamin D status and clinical or metabolic features in this group. Methods: We recruited 38 women with PCOS using the Rotterdam criteria. A total of 109 premenopausal control women were matched with patients based on age and body mass index. Serum 25-hydroxy vitamin D concentrations less than 20 ng/mL were classified as frank vitamin D deficiency. Since vitamin D may play a significant role in metabolic disturbances in women with PCOS, correlations between clinical or metabolic parameters and vitamin D status were analyzed separately in patients and controls. Results: Women with PCOS showed no differences in the level of 25-hydroxy vitamin D ($19.6{\pm}6.6ng/mL$ in patients vs. $20.1{\pm}7.4ng/mL$ in controls, respectively, p=0.696) or prevalence of vitamin D deficiency (57.9% in patients vs. 56.5% in controls, respectively, p=0.880). In addition, we did not find any correlations between serum vitamin D level and clinical or metabolic profiles in either PCOS patients or controls. Conclusion: Our study found no differences in the absolute level of serum vitamin D between PCOS patients and matched controls. Prevalence of vitamin D deficiency was equally common among both patients and controls. Additionally, we did not find any correlations between serum vitamin D level and clinical or metabolic profiles, suggesting that the role of vitamin D in the pathogenesis of PCOS is not yet clear.

Establishing Quantitative Standards for Residual Alkaline Phosphatase in Pasteurized Milk

Kim, Dong-Hyeon,Chon, Jung-Whan,Lim, Jong-Soo,Kim, Hong-Seok,Kang, Il-Byeong,Jeong, Dana,Song, Kwang-Young,Kim, Hyunsook,Kim, Kwang-Yup,Seo, Kun-Ho Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.2

The alkaline phosphatase (ALP) assay is a rapid and convenient method for verifying milk pasteurization. Since colorimetric ALP assays rely on subjective visual assessments, their results are especially unreliable near the detection limits. In this study, we attempted to establish quantitative criteria for residual ALP in milk by using a more objective method based on spectrophotometric measurements. Raw milk was heat-treated for 0, 10, 20, 30, and 40 min and then subjected to ALP assays. The quantitative criteria for residual ALP in the milk was determined as 2 μg phenol/mL of milk, which is just above the ALP value of milk samples heat-treated for 30 min. These newly proposed methodology and criteria could facilitate the microbiological quality control of milk.

Genomic and transcriptomic landscape of <i>Escherichia coli</i> BL21(DE3)

Kim, Sinyeon,Jeong, Haeyoung,Kim, Eun-Youn,Kim, Jihyun F.,Lee, Sang Yup,Yoon, Sung Ho Oxford University Press 2017 Nucleic acids research Vol.45 No.9

<P><B>Abstract</B></P><P><I>Escherichia coli</I> BL21(DE3) has long served as a model organism for scientific research, as well as a workhorse for biotechnology. Here we present the most current genome annotation of <I>E. coli</I> BL21(DE3) based on the transcriptome structure of the strain that was determined for the first time. The genome was annotated using multiple automated pipelines and compared to the current genome annotation of the closely related strain, <I>E. coli</I> K-12. High-resolution tiling array data of <I>E. coli</I> BL21(DE3) from several different stages of cell growth in rich and minimal media were analyzed to characterize the transcriptome structure and to provide supporting evidence for open reading frames. This new integrated analysis of the genomic and transcriptomic structure of <I>E. coli</I> BL21(DE3) has led to the correction of translation initiation sites for 88 coding DNA sequences and provided updated information for most genes. Additionally, 37 putative genes and 66 putative non-coding RNAs were also identified. The panoramic landscape of the genome and transcriptome of <I>E. coli</I> BL21(DE3) revealed here will allow us to better understand the fundamental biology of the strain and also advance biotechnological applications in industry.</P>

Metabolic engineering of <i>Corynebacterium glutamicum</i> for the production of glutaric acid, a C5 dicarboxylic acid platform chemical

Kim, Hee Taek,Khang, Tae Uk,Baritugo, Kei-Anne,Hyun, Sung Min,Kang, Kyoung Hee,Jung, Sol Hee,Song, Bong Keun,Park, Kyungmoon,Oh, Min-Kyu,Kim, Gi Bae,Kim, Hyun Uk,Lee, Sang Yup,Park, Si Jae,Joo, Jeong Elsevier 2019 Metabolic engineering Vol.51 No.-

<P><B>Abstract</B></P> <P> <I>Corynebacterium glutamicum</I> was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. <I>C. glutamicum gabT</I> and <I>gabD</I> genes and <I>Pseudomonas putida davT</I> and <I>davD</I> genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in <I>C. glutamicum</I> for the construction of a glutaric acid biosynthesis pathway along with <I>P. putida davB</I> and <I>davA</I> genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant <I>C. glutamicum</I> was engineered by examining strong synthetic promoters P<SUB>H30</SUB> and P<SUB>H36</SUB>, <I>C. glutamicum</I> codon-optimized <I>davTDBA</I> genes, and modification of <I>davB</I> gene with an N-terminal His<SUB>6</SUB>-tag to improve the production of glutaric acid. It was found that use of N-terminal His<SUB>6</SUB>-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered <I>C. glutamicum</I> H30_GA<SUB>His</SUB> strain, expressing <I>davTDA</I> genes along with <I>davB</I> fused with His<SUB>6</SUB>-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of <SMALL>L</SMALL>-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>C. glutamicum</I> strains were metabolically engineered for the production of glutaric acid. </LI> <LI> Optimization of glutaric acid biosynthesis pathway could enhance glutaric acid production. </LI> <LI> Recombinant <I>C. glutamicum</I> strains efficiently produced glutaric acid from glucose. </LI> <LI> Fed-batch culture of recombinant <I>C. glutamicum</I> H30_GA<SUB>His</SUB> supported high-level production of glutaric acid from glucose. </LI> </UL> </P>