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윤덕영 ( Deok Young Yoon ),권효식 ( Hyo Shik Kwon ) 충북대학교 과학교육연구소 2015 과학교육연구논총 Vol.30 No.1
An Amperometric sensor for dopamine has heen made by immobiling Ramaria botrytis (Pers. ex Fr) Ricken tissue in carbon paste. The sensor presented a higher response for dopamine in 0.030M acetate buffer at pH 4.85, with an applied potential of -0.15V. A detection limite of the electrode was 2.17×10-6M dopamine. The result of this biosensor study using root tissue of chard can be utilized as the basic information for the determination of dopamine.
오준상,Deok-Hyo Yoon,Bhushan Shrestha,Hyung-Kyoon CHOI,Gi-Ho Sung 한국미생물학회 2019 The journal of microbiology Vol.57 No.1
Cordyceps militaris is a species of Cordyceps that is classified in the Cordycipitaceae family and is well known in East Asia as a traditional medicinal mushroom. Its artificial fruit body has been widely cultivated for commercial use in cosmetics, functional food, and medicine. To explore the metabolites associated with fruit body development, we conducted gas chromatography mass spectrometry (GC-MS) analyses based on developmental stage, which was divided into the growth period (stage 1, stage 2, and stage 3) and aging period (stage 4). We detected 39 biochemical metabolites associated with nucleotide, carbohydrate, and amino acid metabolism. Cordycepin, one of the representative bioactive compounds in C. militaris, was significantly enriched in stage 4 of aging period and is associated with glucose accumulation. The accumulation of cordycepin in stage 4 of aging period also seems to be related to the glutamine and glutamic acid pathway. Our results also showed enrichment of other bioactive compounds such as mannitol and xylitol in stage 4 of aging period. Our metabolomic profiling based on the developmental stages of C. militaris is useful for exploring bioactive compounds (e.g., cordycepin, mannitol, GABA, and xylitol) that are enriched in stage 4 of aging period and understanding the biosynthetic mechanisms associated with cordycepin production. Through optimization of fruit body cultivation by selecting stage 4 of aging period as a harvesting time, our findings can be utilized in food and medical applications of C. militaris in future.
종양과 연관되어 발생한 근병증 2예 요약제목: 종양관련 근병증 2예
이효덕 ( Hyo Deok Lee ),김미진 ( Mee Jin Kim ),서의근 ( Eui Geun Seo ),채희복 ( Hee Bok Chae ),한정호 ( Joung Ho Han ),윤순만 ( Soon Man Yoon ),박선미 ( Seon Mee Park ),윤세진 ( Sei Jin Youn ),김대곤 ( Dae Ghon Kim ) 전북대학교 의과학연구소 2012 全北醫大論文集 Vol.36 No.1
저자들은 근병증 2예와 병발한 악성 종양을 경험하였으며, 향후 원인 미상의 간 효소 수치 상승을 가진 환자가 피부 병변과 근력 약화를 호소하는 경우 꼭 근육염 가능성을 염두에 두어야 한다는 교훈을 얻을 수 있었다. 아울러 이러한 환자에서 근육염 확진을 위한 근육 생검, 근염 특이 자가 항체 검사 등을 통해서 IIM으로 진단될 경우 종양의 동반유무를 찾기 위한 종양표지자 및 영상 검사와 함께 PET 등의 첨단 검사도 이용될 필요가 있다는 것을 저자들은 본 2예의 증 례보고를 통해서 주장하는 바이다. 감사의 글 본 증례 환자들의 치료 과정을 함께 해 주시고, 원고를 검토해주신 류마티스 내과 강귀영 교수님께 진심으로 감사 드립니다. It is assumed that malignancy is a part of a clinical course in patients with idiopathic inflammatory myopathy (IIM), as the cancer occurrence in the group have been frequently reported. This paper discusses two cases of IIM associated with cancer either simultaneously or sequentially. The first patient who had diagnosed with colon cancer two weeks prior to the admission presented with polymyositis (PM). The second patient with breast cancer presented dermatomyositis (DM). Both patients were admitted to the hepatology unit because their initial presentation was abnormal liver enzyme levels with muscle weakness. IIM patients can be misdiagnosed as acute hepatitis because of the initial feature. This paper reports two cases of IIM with malignancy.
김호경,김태웅,Deok-Hyo Yoon,Chun-Hoi Kim,Bhushan Shrestha,장우철,So-yeon Lim,Won-Ho Lee,Je-O Lee,Mi-Hee Lim,Geun-Young Kim,최성아,송원옥,Jae-Mo Sung,황기철,Sang-Guk Han 한국식품영양과학회 2007 Journal of medicinal food Vol.10 No.1
Inonotus obliquus(Pers.:Fr.) Pil. is a white rot fungus that belongs to the family Hymenochaetaceae of Ba-sidiomycetes. Extracts and fractions of this fungus have been known to have biological activities, including antimutagenic,anticancer, antioxidative, and immunostimulating effects. Recently, there have been reports that the anti-inflammatory and an-tinociceptive properties of the methanol extract of I. obliquusmay be due to the inhibition of inducible nitric oxide (NO) syn-thase (iNOS) and cyclooxygenase-2 (COX-2) expression via the down-regulation of nuclear factor .B (NF-.B) binding ac-tivity. However, the effects of I. obliquusmediator production have not yet been elucidated. In the present study, a 70% ethanol extract of I. obliquus(IOE70) showedantioxidative effects. We also tested the ability of the I. obliquusextract to inhibit the inflammatory cascades in lipopolysac-charide (LPS)-induced RAW 264.7 macrophage cells. The NO inhibition of IOE70 was better than that of other ethanol ex-tracts from I. obliquus. To investigate the mechanism by which IOE 70 inhibits NO production and iNOS and COX-2 ex-pression, we examined the activations of I.B., Akt, and c-Jun NH2-terminal kinase (JNK) in LPS-activated macrophages.IOE70 markedly inhibited the phosphorylation of I.B.macrophages. Taken together, these experiments demonstrated that IOE70 inhibition of LPS-induced expression of iNOS andCOX-2 protein is mediated by Akt and JNK. Based on our findings, the most likely mechanism that can account for this bi-ological effect of IOE70 involves the inhibition of NF-.B through the phosphatidylinositol 3-kinase/Akt/I.B pathway and theinhibition of JNK activation. Thus, IOE70 might have useful clinical applications in the management of inflammatory dis-eases and may also be useful as a medicinal food.
Kim, Tae Yoon,Seo, Hyo-Deok,Lee, Joong-jae,Kang, Jung Ae,Kim, Woo Sik,Kim, Hye-Min,Song, Ha-Yeon,Park, Ji Min,Lee, Dong-Eun,Kim, Hak-Sung Elsevier 2018 Journal of controlled release Vol.279 No.-
<P><B>Abstract</B></P> <P>Small-sized non-antibody scaffolds have attracted considerable interest as alternatives to immunoglobulin antibodies. However, their short half-life is considered a drawback in the development of therapeutic agents. Here we demonstrate that a homo-dimeric form of a repebody enhances the anti-tumor activity than a monomeric form through prolonged blood circulation. Spytag and spycatcher were genetically fused to the C-terminus of a respective human IL-6-specific repebody, and the resulting two repebody constructs were mixed at an equimolar ratio to produce a homo-dimeric form through interaction between spytag and spycatcher. The homo-dimeric repebody was detected as a single band in the SDS-PAGE analysis with an expected molecular size (78 kDa), showing high stability and homogeneity. The dimeric repebody was shown to simultaneously accommodate two hIL-6 molecules, and its binding affinity for hIL-6 was estimated to be comparable to a monomeric repebody. The serum concentration of the dimeric repebody was observed to be about 5.5 times higher than a monomeric repebody, consequently leading to considerably higher tumor suppression effect in human tumor xenograft mice. The present approach can be effectively used for prolonging the blood half-life of small-sized protein binders, resulting in enhanced therapeutic efficacy.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Synthesis and Cell Death Mechanism of a Militarin Derivative
Fei Chen,Deok Hyo Yoon,Dinneswara Reddy,Changwoo Han,최성아,조재열,성기호,황기철,김태웅,박해일 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.3
Militarin, a novel anticancer substance from a caterpillar-grown traditional insect-flower (Cordyceps militaris), was previously isolated and characterized as an ether compound of 4-tert-octylphenol and a nonaethylene glycol. Among militarin analogs with different numbers of ethylene glycol substructure, the analog with four ethylene glycol substructure (M4EG) exhibited potent anticancer activities. A facile and scalable synthetic pathway of the M4EG was developed and the mechanism of cell death by the M4EG against lung adenocarcinoma epithelial cell line (A549) was explored. The results of this study showed that apoptosis of M4EG toward A549 cells is mediated by a caspase-dependent death-signaling pathway.