A Novel Transthyretin Gene Mutation in Hereditary Transthyretin Amyloidosis: A Case Series of Met13dup Patients

Kina Jeon,Darae Kim,Meesoon Park,Jin-Oh Choi,Kihyun Kim,Seok Jin Kim,Jung-Sun Kim,Eun Seok Jeon 대한심장학회 2023 Korean Circulation Journal Vol.53 No.5

Leaf Proteome and Metabolome Analysis of Wheat-Rye Translocation Lines

A-Reum Kim,Juhyun Nam,Sang-Oh Kwon,Da-Eun Kim,Myeong-Won Oh,Kina Yun,Minhwa Park,Geum Sook Hwang,Yong-Weon Seo,Jong-Soon Choi,Sun-Hee Woo 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Wheat-rye translocation lines were developed to produce a main crop resistant to biological and physical stress. 'Chaupon' rye contains 2RL chromatin to harbor resistance genes for powdery mildew and leaf rust. In order to identify chromosome 2RL-derived rye proteins and 2RL-perturbed proteins in wheat-rye translocation lines, the gel-based proteomics was employed with 'Coker797' (non-2RL), 'Hamlet' (2RL) and 'near-isogenic line' (stabilized 2RL). The leaf proteome was resolved on 2D-gel, resulting in 216 spots in a final selection. A total of 90 proteins were identified with the identification success rate of 42%. The identified proteins were classified by functional annotation: metabolism (64%), cellular process (5%), translation (2%), regulatory function (1%) and hypothetical (28%). The proteins belonged to metabolism were subdivided into carbohydrate metabolism (36%), energy metabolism (35%), metabolism of lipid, amino acid, other amino acid and biosynthesis of secondary metabolites (each 6%) and others (5%). A total of 53 proteins were differentially expressed, in which β-glucosidase, in particular, originated from the chromosome 2RL of rye, was exclusively appeared in NIL. In addition, small Ras-related GTP binding-protein assigned to wheat was predominantly found in 2RL rye chromatin-possessing NIL. These results suggest that the acquired genetic traits obtained from rye 2RL enhance the resistance to biotic and abiotic stress in wheat-rye translocation lines by altered the proteome expression. In leaf metabolome analysis, 11 predominant metabolites containing trans-aconitate, glutamate, and betaine were identified by 1H-NMR-based metabolite fingerprinting. The overall metabolites pattern of NIH appears to be closer to Coker797 rather than Hamlet. Thus, the metabolic phenotype of NIL was not so much lineated from Hamlet contrast to proteomic phenotyping.

국내 흑염소에서의 소결핵, 브루셀라, Q fever 유병률 연구

김효비,김성준,김기나,김별,장병준,최농훈,Kim, Hyobi,Kim, Seongjoon,Kim, Kina,Kim, Byeol,Chang, Byungjoon,Choe, Nong-Hoon 대한수의학회 2016 大韓獸醫學會誌 Vol.56 No.4

As the meat of black goats has become popular as a healthy food, domestic goat meat-related industries are steadily growing. However, previous studies are scarce of informations about the zoonotic disease originated from the black goat in Korea. In this study, we investigated Korean black goat's infectious diseases representing bovine tuberculosis, brucellosis, and Q fever. One hundred and eighty samples were collected from a local slaughter house located in Jeollanam-do. Three typical zoonotic diseases were separately examined by carrying out enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Histopathological test was additionally performed in tuberculosis. In case of tuberculosis, results of the PCR and histopathological test were negative but the ELISA results were positive in eight samples. In case of brucellosis, one out of the total samples was shown to be positive in the ELISA and none in the PCR. In case of Q fever, there were forty one positive in the ELISA and twenty positive in the real-time PCR. Those results indicate that the Korean black goat could be a natural reservoir in the possible chain-infections among human, cows and goats. Thus, further study needs in order to improve productivity as well as to prevent the zoonosis spreading and circulation of other livestock with the black goat in this country.

Rho Guanine Nucleotide Exchange Factor 4 (Arhgef4) Deficiency Enhances Spatial and Object Recognition Memory

유기서,Kina Lee,이용석,Won-Jong Oh,Hyong Kyu Kim 한국뇌신경과학회 2020 Experimental Neurobiology Vol.29 No.5

Guanine nucleotide exchange factors (GEFs) play multiple functional roles in neurons. In a previous study, we reported that Arhgef4 (Rho guanine nucleotide exchange factor 4) functioned as a negative regulator of the excitatory synaptic function by sequestering postsynaptic density protein 95 (PSD-95). However, the role of Arhgef4 in behavior has not been examined. We performed comprehensive behavioral tests in knockout (KO) mice to investigate of the effects of Arhgef4 deficiency. We found that the expressed PSD-95 particle size was significantly increased in hippocampal neuronal cultures from Arhgef4 KO mice, which is consistent with the previous in vitro findings. Arhgef4 KO mice exhibited general motor activity and anxiety-like behavior comparable to those of the wild type littermates. However, spatial memory and object recognition memory were significantly enhanced in the Arhgef4 KO mice. Taken together, these data confirm the role of Arhgef4 as a negative synaptic regulator at the behavioral level.

폐경여성에서의 내시경하 수술의 적응증

우이키나 ( Kina Uy ),김태희 ( Tae Hee Kim ),이해혁 ( Hae Hyeog Lee ),정수호 ( Soo Ho Chung ),이병익 ( Byoung Ick Lee ) 대한폐경학회 2010 대한폐경학회지 Vol.16 No.1

연구목적: 폐경여성에서 자궁 내시경 수술의 적응증을 알아보고자 한다. 연구재료 및 방법: 2001년 3월부터 2009년 3월까지 순천향대학교 부천병원에서 내시경수술을 시행한 시행한 43명을 중심으로 후향적으로 환자의 임상적 특징, 검사결과, 수술 후에 합병증과 결과에 대해서 조사하였다. 결과: 내시경 수술을 통해서 내막조직검사 26명, 내막용종제거술 8명, 자궁근종제거술 7명, 비정상 자궁출혈과 자궁내막증식증 치료 1명, 자궁내종괴 1명, 자궁근종의 평균 크기는 3.2 cm, 재원기간은 평균 2일, 수술 후에 빈혈 수치는 1.1 g/dl이고, 평균 수술 시간은 36분이었다. 결론: 자궁내시경 수술의 적응증은 광범위하게 변화하고 있으며 순수한 자궁내근종, 자궁내막 하 근종, 자궁내막용 중, 자궁선근종, 자궁내막증식증, 물묵자궁증, 비정상자궁출혈이 내시경하 수술로 치료될 수 있다. 자궁내시경수술 시복부초음파가 수술시의 합병증을 줄인다. Objectives: To evaluate the spectrum of hysteroscopic surgery to be extended. Methods: Forty-three women who underwent hysteroscopic procedures with Urione(R) solution or normal saline as distension media between March 2001 and March 2009 were randomized. The clinical characteristics, laboratory data, and post-operative results were retrospectively analyzed. Results: The following hysteroscopic procedures were performed: endometrial biopsy, 26; endometrial polypectomy, 8; myomectomy, 7; ablation for dysfunctional uterine bleeding and endometrial hyperplasia, 1; and excision of an endometrial mass, 1. The mean size of the myomas was 3.2 cm. The average duration of hospitalization was 2 days. The average change in hemoglobin was 1.1 g/dl. The mean operative time was 36 minutes. Conclusion: The indications for hysteroscopic procedures can be extended. Pure intramural myomas, submucosal myomas, endometrial polyps, adenomyosis, endometrial hyperplasia, hydrometra, and abnormal uterine bleeding can be treated via a hysteroscope. Intra-operative transabdominal ultrasonographic guidance can be helpful during hysteroscopic procedures, and reduces the complication and morbidity rates. (J Korean Soc Menopause 2010;16:46-51)

The Effects of Group Cognitive Behavioral Therapy on the Improvement of Depression and Anxiety in Adolescents with Problematic Internet Use

Sang-Hyun Kim,Hyeon-Woo Yim,Sun-Jin Jo,Kyu-In Jung,Kina Lee,Min-Hyeon Park 대한소아청소년정신의학회 2018 소아청소년정신의학 Vol.29 No.2

Objectives: The overuse of the Internet among adolescents has increased dramatically in recent years, leading to pathological or problematic Internet use. Cognitive behavioral therapy (CBT) is known to be effective for the treatment of problematic Internet use, particularly for adolescents. The aim of the present study was to evaluate the therapeutic efficacy of group CBT for problematic Internet use in adolescents. Methods: A total of 17 patients aged 12–17 years who met Young’s diagnostic questionnaire criteria of problematic Internet use participated in a school-based eight-session group CBT program. The level of problematic Internet use among participating students was measured using Young’s Internet Addiction Scale (IAS). Depression and anxiety levels were evaluated using the Children’s Depression Inventory (CDI) and the State-Trait Anxiety Inventory (STAI), respectively. Each construct was assessed at baseline, immediately after the intervention, and at a one-month follow-up visit. Statistical significance was based on a p-value of <0.05. Results: Immediately after the program, the IAS, CDI, and State Anxiety Inventory (SAI) scores were significantly lower than before the program. At the one-month follow-up assessment, the IAS scores remained low, and the CDI and SAI scores were even lower than immediately after the program. Conclusion: Group CBT was effective for adolescents with problematic Internet use, and was also demonstrated to improve depression and anxiety.

Immunogenicity of SARS-CoV-2 Vaccine in Kidney Transplant Recipients: A Cross-Sectional Study in Korea

송승환,정구용,Jee Yongho,Chung Hae-Sun,Kim Kina,Minn Dohsik,Kim Soo-Kyung 대한의학회 2023 Journal of Korean medical science Vol.38 No.5

Eighty-five Korean kidney transplant recipients who received three doses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine were tested with anti-receptor binding domain (RBD) antibody and neutralizing antibody. High anti-RBD antibody (≥ 100 U/mL) and neutralizing antibody responses (≥ 30%) were detected in 51/85 (60.0%) patients. When we divided the patients with the time from transplantation to vaccination (< 1, 1–2.4, 2.5–4.9, and ≥ 5-year), anti-RBD antibody titers were 3.2 U/mL, 27.8 U/mL, 370.2 U/mL, and 5,094.2 U/mL (P < 0.001) and anti-neutralizing antibody levels were 2.2%, 11.6%, 45.6%, and 93.0% (P < 0.001), respectively. Multivariate analysis revealed increased antibody responses when the time from transplantation to vaccination was five years or longer (odds ratio, 12.0; confidence interval, 2.7–52.8). Korean kidney transplant recipients had suboptimal antibody responses after the third dose of SARS-CoV-2 vaccine. A shorter time from transplantation to vaccination was a risk factor for a low antibody response.