Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

도시공원 범죄예방을 위한 CPTED 인증평가기준 정립에 관한 연구

김강일(Kang Il Kim),조준택(Joon Tag Cho),박현호(Hyeon Ho Park) 한국셉테드학회 2018 한국셉테드학회지 Vol.9 No.1

도시지역에서의 공원이란 시민들의 건강과 휴양 그리고 정서생활에 도움을 주고 녹지를 확보하여 환경에 도움을 주는 공간이다. 하지만 누구에게나 개방되어 있는 도시 공원의 특성상 공간의 안전을 위협하는 소수의 위협들은 다수의 이용자인 시민들에게 불편함을 주거나 위협을 초래하는 경우가 발생한다. 이와 관련하여 각종 선행 연구들은 공원에서 범죄가 빈번하게 발생하고, 시민들의 휴식공간이라는 공원의 제 기능을 다 하지 못하고 있다는 문제를 지적하고 있다. 특히 최근 많은 관심을 받고 있는 사회적 약자를 대상으로 한 범죄에 대응하기 위한 도시공원의 설계 관리가 중요함을 일관되게 강조하고 있다. 이에 이 연구에서는 CPTED의 기본이론을 토대로 도시공원의 범죄예방과 관련된 선행 연구를 분석하여 도시공원에 적합한 CPTED 요소를 추출하고 도시공원의 방범환경 인증평가 체크리스트를 개발하였다. 이러한 체크리스트를 바탕으로 크기별, 종류별 공원 5곳을 선정하여 실제 현장 조사를 실시하고 안전요소를 분석하여 최종 체크리스트를 도출하였다. 이 체크리스트의 최종평가분야는 관리운영, 감시, 접근통제, 영역성, 활동성 지원 CPTED요소를 기반으로 하고 이에 더하여 안내표지, 방범 및 안전시설, 가점항목으로 설정하였으며 각각의 세부평가항목으로 구성하였다. 이 연구에서 개발한 체크리스트의 평가기준은 모든 공원에 대한 범죄예방 인증평가를 수행할 수 있도록 하는 범용 체크리스트로 이러한 평가기준은 다양한 규모와 형태를 가진 공원들의 특성을 고려하여 평가와 인정의 기본원칙에 의거하여 다양한 형태로 변형되어 활용할 수 있도록 하였다. 이와 관련하여 범죄위험을 예방하는데 있어 중요한 분야의 핵심 항목들에 대해서는 높게 배점하고 정성적인 평가에 의해 배점을 차등적으로 주도록 하였다. 또한 신규 공원뿐만 아니라 기존에 있던 공원들도 적극적 개선을 통해 인정을 받게 하고자 하는 취지로 만들어 전체적인 공원 안전개선을 위한 기반이 되도록 하였다. 본 체크리스트는 경찰청이 범죄예방진단팀(CPO)을 통해 시행 중인 도시공원의 범죄예방우수시설 인증사업에 활용하고 있다. Urban public parks are supposed to be the places for citizens’ rest, healing and public health. However, as public parks are usually open for 24 hours almost every day they are often exposed to criminal attacks within the realm of the space. The crime problem therefore threatens the public safety and security. Previous studies on public parks and crime safety continuously indicate that urban parks are vulnerable to crime and disorder and became the cause of fear of crime due to poor environmental design and planning in terms of CPTED. In this context the researchers have developed a checklist for accreditation/certification of urban public parks for crime prevention by analyzing the examples applying the CPTED principles in public parks and reflecting the field survey results in South Korea. In regard to the item and point, the crucial points for public parks rated highly and the grading was differentiated by qualitative evaluation. In addition, basic principles for certification of Secure/Safe Public Parks Awards were suggested, including operating a rating system to certify public parks so as to promote efforts for improvement, not awarding the certification to parks if any relevant regulations are violated, applying the evaluation items or points in accordance with the local context. The assessment checklist can be revised and used in various ways according to the regional circumstances and can contribute to the security and safety of public parks. The Police Crime Prevention Officers (CPO) are to utilize this checklist for the Secure Public Parks Certification Awards program in order to enhance the level of security for public parks in major cities in South Korea.

태안해안국립공원 지역특화자원을 활용한 탐방프로그램 개발 연구

배준호(Joon-ho Bae),남승민(Seung-min Nam),허학영(Hak-young Heo),송창룡(Chang-ryong Song),박현숙(Hyun-suk Park) 국립공원연구원 2021 국립공원연구지 Vol.12 No.1

본 연구를 위해 먼저 국내·외 해상·해안국립공원의 운영프로그램 현황에 대한 조사를 시행하였으며, 조사된 일부 사례의 경우 기후 및 지형적 요인, 그에 따른 생태환경의 차이, 그리고 관련 법령 차이 등의 이유로 즉시 국립공원에 도입하기 보다는 중·장기적 운영계획과 인프라의 확충, 전문성 확보 등 체계적인 준비가 필요한 것으로 나타났다. 보다 적합한 탐방프로그램의 개발을 위해 사업대상지 현장방문을 실시하였으며, 기존 운영프로그램 현황, 각종 시설, 인력현황 파악, 주변 환경 분석, 담당자 인터뷰 등을 통해 맞춤형 탐방프로그램을 구성하였다. 태안해안국립공원의 경우, 연구원 협업 철새 가락지 부착체험, 태안 해변길 - 솔모랫길 탐방, 태안해변 드론 탐방, 마음의 의자, 연날리기, 새와 예술 그리고 … 등의 체험형 프로그램과 한서대학교와 함께하는 해양 안전 교육 프로그램, 유류 오염 교육 프로그램 등의 교육형 프로그램 등 모두 8가지 해양국립공원 운영프로그램이 도출되었다. 제안된 탐방프로그램을 운영하기 위해서는 별도의 장소 및 운영시설의 확충, 예산편성, 운영을 위한 타당성 검토 등이 우선되어야 할 것이다. 특히 탐방프로그램을 효율적으로 운영하기 위해서는 프로그램 지도자의 역량개발이 필요하며, 현재 운영되고 있는 프로그램을 보완하고 새로운 프로그램을 시행하기 위한 추가적 논의가 필요할 것으로 사료된다. For this study, we first examined the currently operating programs of domestic and overseas maritime and costal national parks. It was found that some of the programs require systematic preparations such as mid- and long-term operation plans, infrastructure expansion, and maintenance of professionalism rather than immediately introducing them into national parks due to the differences in climatic and geographic factors, ecological environment, and relevant regulations. To develop more suitable programs, on-site visits were made and the customized programs were suggested by closely examining the status quo and interviewing people in charge of running the currently offered programs. For Taean Coastal National Park, eight programs were recommended as feasible ones, including the experiential programs such as Bird Ringing, Taean Beach Trail - Sol Sand Trail Walking, Travel Taean Beach with Drone, Thinking Chair, Kite Flying, and Culture & Arts Program (Birds, Arts, and …), and the educational programs such as Marine Safety Training and Oil Pollution Awareness Program. For the suggested programs to be realized, the expansion of separate places and operating facilities for each program, budgeting, and feasibility study should be preceded. To operate the programs effectively and efficiently, in particular, it is necessary to develop the competency of program leaders, and additional discussion for supplement the currently operated programs and implement new programs.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes

Kim, Se-Jin,Ho Hur, Joon,Park, Channy,Kim, Hyung-Jin,Oh, Gi-Su,Lee, Joon No,Yoo, Su-Jin,Choe, Seong-Kyu,So, Hong-Seob,Lim, David J,Moon, Sung K,Park, Raekil Nature Publishing Group 2015 Experimental and molecular medicine Vol.47 No.2

<P>Bucillamine is used for the treatment of rheumatoid arthritis. This study investigated the protective effects of bucillamine against cisplatin-induced damage in auditory cells, the organ of Corti from postnatal rats (P2) and adult Balb/C mice. Cisplatin increases the catalytic activity of caspase-3 and caspase-8 proteases and the production of free radicals, which were significantly suppressed by pretreatment with bucillamine. Bucillamine induces the intranuclear translocation of Nrf2 and thereby increases the expression of γ-glutamylcysteine synthetase (γ-GCS) and glutathione synthetase (GSS), which further induces intracellular antioxidant glutathione (GSH), heme oxygenase 1 (HO-1) and superoxide dismutase 2 (SOD2). However, knockdown studies of HO-1 and SOD2 suggest that the protective effect of bucillamine against cisplatin is independent of the enzymatic activity of HO-1 and SOD. Furthermore, pretreatment with bucillamine protects sensory hair cells on organ of Corti explants from cisplatin-induced cytotoxicity concomitantly with inhibition of caspase-3 activation. The auditory-brainstem-evoked response of cisplatin-injected mice shows marked increases in hearing threshold shifts, which was markedly suppressed by pretreatment with bucillamine <I>in vivo</I>. Taken together, bucillamine protects sensory hair cells from cisplatin through a scavenging effect on itself, as well as the induction of intracellular GSH.</P>

대학의 교수학습 개발에 대한 요구분석 연구 : 동국대학교 교수집단을 중심으로 focusing on Faculties of the Dongguk University

박명희,김성훈,박명숙,이중권,박종호,이성림 동국대학교 교육연구원 2005 교육문제연구 Vol.16 No.-

본 연구의 목적은 동국대학교의 교수들이 수행하고 있는 교수학습 방식을 파악하고 각 방법들의 효과성을 교수들이 어떻게 인식하고 있는지를 알아 보고자 하였으며 창의적 교수학습방법개발에 대한 교수들의 요구를 파악하여 교수학습개발센터의 프로그램개발을 위한 기초자료로 삼고자 하였다. 수업방식에 있어서 전공, 교양을 막론하고 대부분이 강의식 수업방식이 높았으며(80.9%, 86%), 발표식, 토론식이 (14%, 18.6%) 실험실습 등이 그 다음으로 나타났다. 교수들은 전공과목보다는 교양수업에서 학생들의 요구에 맞는 수업 수업방식을 고려하고 있는 반면 수업방식의 효과에 대해 53%만이 효과적이라고 보고하고 있다. 교수들도 효과적인 강의방법 등 교수법에 대해 체계적으로 학습할 필요성을 느끼고 있었다. 강의 기술(교수방법)과 토론 중심 수업 방법에 대한 워크샵에 대한 요구가 가장 높게 나타났고 발표수업과 프로젝트수업 방법 등 강의식 수업 이외의 다른 수업방식에 대한 워크샵 요구도가 높았다. 그 외에 학교의 지원에 대해서는 첨단 수업 기자재 활용을 위한 보조자(조교)에 대한 요구도가 가장 높고 강의 운영을 위한 보조금, 개인별 상담 지원 순으로 요구도가 높게 나타났다. The purpose of this study was to comprehend the methods of teaching and learning carried out by the teachers of Dongguk University, and to find out how the professors found those methods effective. This research was also used to serve as a basis for the development of a more creative teaching-learning system to enhance better programs for the Professor Teaching-Learning Development Center. In teaching methods, regardless of classes for majors or for cultural enrichment, most of the courses were in the form of lectures (80.9%, 86%) followed by research and discussion (14%, 18.6%), with experiment and practical training coming in third. Although professors tend to choose a more student-oriented class method in distribution courses more than in major courses, it was reported that only 53% of the faculty claimed this class method to be effective. The faculty felt the need for professors to have more constructive learning in incorporating more effective teaching methods. The demand for professor workshops on teaching skills (teaching methods) and discussion oriented classes proved to be high, as well as the need for workshops on teaching methods other than lectures, such as research courses and project oriented class methods. Regarding other demands for university support, the demand for an assistant (assistant teacher) to help with the new technology equipment was the highest, followed by subsidies for course management and support in individual counseling.

장애 평가 후 5년 경과된 외상성 뇌손상 환자의 정신사회적 기능에 대한 연구

박준순,노승호 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.5

Objectives : This study was designed to investigate the long-term psychosocial outcome of the patients with traumatic brain injury (TBI) about five years after initial assessment of disability. The psychosocial outcome was evaluated in the areas of cognitive function, neurobehavioral symptoms, socio-occupational function, purpose of life, and quality of life. Methods : Twenty seven patients with TBI (TBI-S) were selected, who had admitted to the department of Neuropsychiatry of the Wonkwang University Hospital with a primary diagnosis of TBI for the initial assessment of disability during the period 1994-1998. At first, the author assessed their functions with the Neurobehavioral Rating Scale, Social Occupational Functioning Assessment Scale, and Quality of Life Index. And the patients completed the Korean Wechsler Adult Intelligence Scale, Rey-Kim Memory Test and Kims Executive Function Test, the Head Injury Symptom Checklist, Korean version of SmithKline Beecham Quality of Life Scale and Purpose-in-Life Test. The data from the TBI-S were compared between those from the TBI patients (TBI-C) who had received brain injury at the similar time and the TBI-S and admitted for the assessment of disability. Results : Although there was no significant difference between the two TBI groups in the areas of cognitive function, such as generaI intelligence, verbal comprehension, visuo-spatial function, attention, memory, and executive function, the TBI-S showed significantly lower neurobehavioral symptoms, higher socio-occupational functioning, and higher purpose in life and quality of life levels than the TBI-C. Though 5 years had passed since injury the TBI-S still had various emotional and somatic symptoms and remained at the relatively lower levels in psychosocial functioning. Conclusion : The overall long-term psychosocial outcome of the TBI patients depends significantly on the status at the initial assessment of disability about 5 years ago. The improvement was seen in the areas of neurobehavioral symptoms and socio-occupational functioning but not in cognitive function. In spite of the improvement in some areas they still remained at relatively low levels in purpose-in-life and quality of life. This findings suggest that the specialized medical and social support is needed for their rehabilitation.

DBS STB의 스마트 카드 인터페이스 설계

박준호,윤영우 嶺南大學校 工業技術硏究所 2001 工業技術硏究所論文集 Vol.29 No.2

In this paper, we design a Smartcard interface of DBS(Direct Broadcasting by Satellite) receiver that manages resource and subscriber management system by Mugunghwa satellite broadcasting system. The smartcard is used for subscribers selecting TV programs or various services. It must follow ISO/IEC 7816 standard for smartcard and observe Mugunghwa satellite DBS system regulations. Simulation is performed with virtual smartcard through personal computer serial communication. From the simulation results, the designed method is adequate to function of DBS regulations and shows good performance.

SnO_2-ZnO 계 후막센서 구조에 따른 CO 감지 특성

박보석,홍광준,김호기,박진성 한국센서학회 2002 센서학회지 Vol.11 No.3

CO 기체 감지 특성을 향상시키기 위해서 3 mol% ZnO를 첨가한 SnO_2와 3mol% SnO_2를 첨가한 ZnO의 적층 형태를 변화시켜 연구하였다. 적층 구조는 단일층, 복층, 그리고 이종층 구조로 후막 인쇄법을 사용하여 제작하였다. SnO_2-ZnO계에서 제 2상은 발견되지 않았다. 전도성은 SnO_2에 ZnO를 첨가하면 감소하고, ZnO에 SnO_2를 첨가하면 증가하였다. 측정 온도증가와 CO 기체 유입으로 전도성은 증가하였다. 단층 및 복층의 후막센서 구조의 감도 향상은 없었으나, SnO_2 -3ZnO-ZnO · 3SnO_2·substrate 구조의 이종층 센서의 감도는 향상되었다. 센서 구조에 관계없이 I-V 변화는 모두 직선성을 나타내서 Ohmic 접합 특성을 이루고 있었다. The sensing properties of carbon monooxide were investigated as a function of mixing ratio and the lamination structure of 3mol% ZnO-doped SnO_2 and 3mol% SnO_2-doped ZnO. The lamination structures were fabricared monolayer, double layer, and hetero layer of SnO_2, ZnO, and theirs mixture composition using thick film process. There was no second phase by the reaction of SnO_2 and ZnO. The conductance was decreased by the addition of ZnO in SnO_2, but it was increased with the addition of SnO_2 in ZnO. The conductance was increased with temperature and the inlet of CO. There was no improvement of sensitivity in the structure of mono- and double-layer. The hetero-layer structure, however, of SnO_2 · 3ZnO-ZnO · 3SnO_2 showed the higher resistivity and the highest sensitivity. Ohmic characteristics was confirmed by the linear properties for I-V measurements.

퍼지 PI 제어기를 이용한 유도 전동기의 속도 제어에 대한 연구

박준열,전광호 弘益大學校 科學技術硏究所 1997 科學技術硏究論文集 Vol.8 No.-

The conventional PI control, that are used in the control loop for outer speed of the induction motor, also has difficulty dealing with the dynamic speed tracking, parameter variation, and the load disturbance. As a result, the motion control system operates with a poor performance in the motor's low speed range. In this dissertation, a novel speed control system that implements the fuzzy logic controller(FLC) is proposed. The proposed FLC used the speed error, its rate of change, and the drive current as three fuzzy variables. The real command output current is chosen by the defuzzification of the fuzzy output extracted from the fuzzy control rules. The flux and the torque of the induction motor are estimated using the dynamic model with an excellent dynamic response over a wide speed range and with the rotor flux field-oriented scheme of the decoupling characteristics. The performance of this control system is substantiated by computer simulations.