Effect of Dimethylformamide on Post-Thaw Motility, Acrosome Integrity, and DNA Structure of Frozen Boar Sperm

황유진,양재훈,김상옥,김보경,최선규,박춘근,김대영 사단법인 한국동물생명공학회 2009 한국동물생명공학회지 Vol.24 No.4

The beneficial effect of glycerol as a cryoprotectant, especially for sperm cryopreservation, has been shown in many studies. However, glycerol is toxic to living cells, and boar sperm in particular show greater sensitivity to glycerol than sperm from other domestic animals. Amides have been studied as alternative cryoprotectants for freezing stallion sperm. Sperm frozen in methylformamide or dimethylformamide as cryoprotectants show similar motility when thawed compared with sperm frozen in glycerol. We evaluated the cryoprotective effects of dimethylformamide on boar sperm freezing. To test the effect of amides, the concentration of boar semen was adjusted to 109sperm/mL, and seminal plasma was removed using Hulsen solution. After centrifugation, the pellet was diluted in modified-Modena B extender. Lactose-egg yolk (LEY) extender was used as the cooling extender. The freezing extender was madeed aaddition of the optimal amount of glycerol and amides to LEY-Glycerol-Orvus ES Paste extender, and this extender was used for the second dilution. Diluted sperm were frozen in liquid nitrogen using the 0.5 mL straw method. Sperm frozen in extender with glycerol as a cder ol were compared with those frozen in extender including the different amides. Sperm were tested for motility, viability, the sperm chromatin structure assay, and normal apical ridge after thawing. The percent of motile sperm diluted in glycerol was as high as that in the stallion study (61%). Dimethylformamide showed positive effects on sperm quality and was better than glycerol. Methylformamide provided similar sperm quality as glycerol. Therefore, dimethylformamide is useful for reducing cryoinjury in boar sperm and is expected to be useful as an alternative cryoprotectant.

Sex Ratio Determination by Quantitative Real Time PCR using Amelogenin Gene in Porcine Sperm

황유진,배문숙,양재훈,김보경,김상옥,이은수,최선규,권예리,서민혜,박춘근,김대영 사단법인 한국동물생명공학회 2009 한국동물생명공학회지 Vol.24 No.3

Sex-sorting of sperm is an assisted reproductive technology (ART) used by the livestock industry for the mass production of animals of a desired sex. The standard method for sorting sperm is the detection of DNA content differences between X and Y chromosome-bearing sperm by flow cytometry. However, this method has variable efficiency and therefore requires verification by a second method. We have developed a sex determination method based on quantitative real-time polymerase chain reaction (qPCR) of the porcine amelogenin (AMEL) gene. The AMEL gene is present on both the X and the Y chromosome, but the length and sequence of its noncoding regions differ between the X and Y chromosomes. By measuring the threshold cycle (Ct) of qPCR, we were able to calculate the relative frequency of X chromosome. Two sets of AMEL primers were used in these studies. One set (AME) targeted AMEL gene sequences present in both X and Y chromosome, but produced PCR products of different lengths for each chromosome. The other set (AXR) bound to AMEL gene sequences present on the X chromosome but absent esholthe Y-chromosome. Relative product levels were calculated by normalizing the AXR fluorescence to the AME fluorescence. The AMEL method accurately predicted the sex ratios of boar sperm, demonstrating that it has potential value as a sex determination method.

오존을 산화제로 사용한 SO_x, NO_x 입자 전환 메커니즘

황유진,김기훈,김지희,박영권 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.1

XGBoost, LightGBM을 이용한 종목추천 뉴스기사 기반 주식 수익률 방향성 예측

황유진,손승연,이준기 한국컴퓨터정보학회 2024 韓國컴퓨터情報學會論文誌 Vol.29 No.2

투자자는 수익의 극대화를 위해 언론사의 기사를 포함한 다양한 정보를 활용하여 투자 전략을수립한다. 이에 국내 언론사에서도 신뢰도 있는 투자정보를 제공하기 위해, 애널리스트의 종목분석 보고서에 기초한 종목 추천기사를 게재하고 있다. 본 연구에서는 종목 추천기사 게재를 하나의 사건(event)으로 간주하고, XGBoost와 LightGBM 모델을 활용하여 기사 게재 10일 이후 가격의상승 또는 하락을 예측하는 분류 모델을 제시한다. 또한, 전체 추천종목을 유가증권시장과 코스닥시장 및 기업규모(대형/소형)에 따라 4가지로 분류하고, 하위 그룹에 따라 모델의 예측 정확도에차이가 있는지 파악하고자 한다. 학습 결과 전체 모델의 분류 정확도는 XGBoost 75%, LightGBM 71%로 나타났고, 예측 정확도는 유가증권 시장 예측력이 코스닥시장 주식 대비 높게 나타났으며, 대형주의 예측력이 소형주 보다 높게 나타났다. 마지막으로, SHAP(Shapley Additive exPlanations) 분석을 통해 개별 모델의 예측에 중요한 변수를 살펴보고 모델의 해석력을 제고하였다.

벤치 규모 고정층 반응기를 이용한 다양한 리그닌의 열분해 공정 최적화

황유진,정재훈,박영권,( Pouya Sirous Rezaei ) 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0

현대 사회의 지구 온난화 문제를 해결하기 위해 제시된 다양한 방법 중 하나는 대체 에너지원의 개발이다. 화석연료를 대체할 수 있는 에너지원으로 주목 받고 있는 lignocellulosic biomass는 열화학적 전환 공정 중 열분해를 통해 대체 연료인 바이오 오일을 생산할 수 있다. Lignocellulosic biomass를 구성하는 요소 중 하나인 리그닌은 구조적 특징에 의한 낮은 반응성과 열분해 시 agglomeration 현상 때문에 연구가 많이 진행되지 않고 있다. 본 연구에서는 리그닌 열분해를 통해 효과적으로 고부가가치 물질을 추출하기 위해 다양한 방식으로 만들어진 리그닌들을 사용하여 다양한 조건들로 실험하여 벤치 규모 고정층 반응기에서 최적의 열분해 조건을 찾는 연구를 진행하였다. ^** 이 성과는 2017년도 정부(과학기술통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No. 2017M1A2A2087674).

농가 근로환경 개선기획 ② - 우리목장 외국인 근로자 고용, 이것만은 알아두자.

황유진,Hwang, Yu-Jin 한국낙농육우협회 2016 낙농·육우 Vol.36 No.12

Emotion Regulation Strategies as Moderators between Counterfactual Thinking Concerning Intimate Partner Violence and Trauma-related Emotions: An Ecological Momentary Assessment Study

황유진,박수현 한국임상심리학회 2023 Korean Journal of Clinical Psychology Vol.42 No.3

According to the cognitive model of posttraumatic stress disorder (PTSD), negative appraisals and negative emotions are key factors in PTSD symptoms. Moreover, emotion regulation strategies (ERS) may affect the severity of PTSD symptom differently. This study investigated the reported types and frequency of intimate partner violence (IPV) survivors, and the effects of counterfactual thinking (CFT) and ERS on trauma-related emotions in daily life via ecological momentary assessment (EMA). Data from 59 women who experienced IPV within the past year were analyzed. The results demonstrated that cognitive reappraisal may modulate the relationship between upward CFT and trauma-related emotions (B= -0.012, p= .005), but the effect of emotion suppression was not statistically significant (B= -0.006, p= .365). Especially, upward CFT may demonstrate a greater impact on trauma-related emotions in individuals who use a lower degree of cognitive reappraisal in daily life than in participants employing a higher degree. Conversely, although downward CFT also increased trauma-related emotions, neither type of ERS moderated this relationship (cognitive reappraisal: B= -0.069, p= .129; emotion suppression: B= -0.004, p= .947). These findings extend prior research on the effectiveness of cognitive reappraisal by reinforcing its ecological validity and emphasize the need for further investigations.

Expression of Heat Shock Protein 60 kDa Is Upregulated in Cervical Cancer

황유진,이순표,김석영,Young Hwan Choi,Min Ji Kim,Choong Ho Lee,Joo Young Lee,김대영 연세대학교의과대학 2009 Yonsei medical journal Vol.50 No.3

Purpose: Cervical cancer caused by the human papilloma virus (HPV) continues to be the cause of yearly death among women. However, it is a curable disease when diagnosed at an early stage. Recently, several researches have reported that heat shock protein (HSP) 60, a chaperone protein of molecular weight of 60 kDa, is involved in carcinogenesis and apoptosis. In order to evaluate the prognostic significance of HSP60 in cervical cancer, we examined differences in the HSP60 expression between cervical cancer and normal tissues in women. Materials and Methods: Tissue samples were collected from 20 cervical cancer patients and 20 normal controls. HSP60 expression of cervical cancer and normal tissues were verified by the 2D gel proteomics, semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analyses. Results: In 2D proteomic analysis, an increase of HSP60 expression was detected in cervical cancer tissues and confirmed by Western blot analysis (p < 0.05). However, messenger RNA (mRNA) levels of HSP60 did not display any significant differences between cervical cancer and normal tissues. Conclusion: These results suggest that HSP60 may be involved in the development of cervical cancer and have profound biological and prognostic significance.

어린이집과 유치원의 정보공시제에 대한 교사와 학부모의 인식 조사 연구

황유진,서영숙 한국아동학회 2014 한국아동학회 학술발표논문집 Vol.2014 No.11

고선량의 감마선과 전자선이 조사된 cotton linter 종이의 물리적 특성 변화 비교

황유진,정명준,박해준 한국펄프·종이공학회 2022 펄프.종이기술 Vol.54 No.6

The effects of gamma ray and electron beam irradiation on paper disinfection were eval- uated. The paper was irradiated with 10-100 kGy gamma rays and electron beams which were higher than the recommended dose for paper disinfection. After irradiation, the mechanical properties and color of the paper and the cellulose chain scission rate were evaluated. In the case of paper strength, it decreased after 25 kGy irradiation, whereas the discoloration of paper appeared after 10 kGy irradiation. It cannot confirm the effect on paper only with changes in mechanical strength and color in the recommended range of 0.5-10 kGy for actual paper disinfection treatment. On the other hand, even with 10 kGy irradiation, the degree of polymerization of cellulose paper was reduced by ~50%. Cotton linter paper with a degree of polymerization of about 2,000 was predicted to decrease by about 7% after being irradiated with a 1 kGy gamma ray and electron beam. To evaluate the irradiation effect on paper, it is therefore more reasonable to evaluate the degree of polymerization rather than the strength and color of the paper. .